Vector for cloning and expression of Pseudomonas outer membrane protein in E. coli?
In Pseudomonas the signal sequence allows the translocation of the protein across the inner membrane and then in the periplasm the signal gets cleaved off, then the protein gets embedded into the outer membrane because of its intrinsic affinity for the outer membrane. PB22 vector has been used for this type of expression which expresses OM proteins as a fusion with the signal sequence of the E. coli YtfM OM protein.
But I am not able to get this vector. Can someone suggest some other vector for such cloning?