Varital purity analysis through SSR marker

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• 
  Giovanni Larama Denis · Universidad de La Frontera

  Primers must be available in any paper, usually do you select in base of their PIC (polymorphic information content), if it's close to 1, it's most polymorphic, so coincidences between samples must be more significative. There's another factor that will be a problem that is the quality of PCR product (it's very important if you're checking in Polyacrilamide Gels) because in some primers do you see spots, not a well defined band... too bad that almost never information about quality is attached... it's a problem to find little differences in Polyacrilamide gels, other way is using primers marked with fluorophores and Automatic Sequencer If SSR is too problematic, you can always try AFLP or RAPD! (i'm sorry for english mistakes but it's late here!)

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  Primers must be available in any paper, usually do you select in base of their PIC (polymorphic information content), if it's close to 1, it's most polymorphic, so coincidences between samples must be more significative. There's another factor that will be a problem that is the quality of PCR product (it's very important if you're checking in Polyacrilamide Gels) because in some primers do you see spots, not a well defined band... too bad that almost never information about quality is attached... it's a problem to find little differences in Polyacrilamide gels, other way is using primers marked with fluorophores and Automatic Sequencer
  
  If SSR is too problematic, you can always try AFLP or RAPD!
  
  (i'm sorry for english mistakes but it's late here!)

  Mar 18, 2012
• 
  Swapna Deshmukh · Nirmal Seeds Pvt. Ltd.

  Thanks for your guidance.

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  Thanks for your guidance.

  Mar 19, 2012
• 
  Anand Khot ·

  @ Swapna.. I will go with Giovanni It's good suggestion go with it but I have some following doubts and suggestion... 1.Are you going for Parental purity or Hybrid purity???? 2. SSR markers also good source but they are less in nor available publicly.... good luck

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  @ Swapna.. I will go with Giovanni It's good suggestion go with it but I have some following doubts and suggestion...
  1.Are you going for Parental purity or Hybrid purity????
  2. SSR markers also good source but they are less in nor available publicly....
  
  good luck

  Mar 21, 2012
• 
  Swapna Deshmukh · Nirmal Seeds Pvt. Ltd.

  thanks I am Going for both parental as well as hybrid purity.

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  thanks
  
  I am Going for both parental as well as hybrid purity.

  Mar 22, 2012