Question

Sample preparation in PCR machine for SDS-PAGE?

I generally aliquote cell lysate sample in PCR tubes after protein estimation. Can I add loading dye to a sample in PCR tubes and heat the samples at 95C for 5min in PCR machine?
Is it ok doing it like this, Has anyone tried?

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All Answers (2)

  • Ayan Sadhukhan · Indian Institute of Technology Guwahati
    I usually do like this only, 99C for 10min in heating block
  • Thanks Ayan

    so no problem heating sample in PCR machine.but why for 10 mins.generally every one say 5mins .

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