Sample preparation in PCR machine for SDS-PAGE?

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• 
  Ayan Sadhukhan · Indian Institute of Technology Guwahati

  I usually do like this only, 99C for 10min in heating block

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  I usually do like this only, 99C for 10min in heating block

  Jun 20, 2012
• 
  Sri Trisha

  Thanks Ayan so no problem heating sample in PCR machine.but why for 10 mins.generally every one say 5mins .

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  Thanks Ayan
  
  so no problem heating sample in PCR machine.but why for 10 mins.generally every one say 5mins .

  Jun 21, 2012