Performing biological reactions in an anerobic condition

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• 
  Tomáš Hluska · Palacký University of Olomouc

  you will need hood with nitrogen in it. If you have large volume of liquids, you can bubble nitrogen through it. For smaller amounts you can add some strong reductants like merkaptoethanol, DTT, dithionite etc.

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  you will need hood with nitrogen in it. If you have large volume of liquids, you can bubble nitrogen through it. For smaller amounts you can add some strong reductants like merkaptoethanol, DTT, dithionite etc.

  Aug 13, 2012
• 
  Itzhak Fishov · Ben-Gurion University of the Negev

  Bubbling nitrogen or argon is a simplest way, strong reductants may be incompatible with some proteins and reagents in the reaction mixture. One of the most effective and convenient methods, particularly for small volumes, is using an oxygen scavenging system composed of 1% β-D-glucose, 25 units/ml glucose oxidase, and 250 units/ml catalase. For a review of different methods see attached paper.

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  Bubbling nitrogen or argon is a simplest way, strong reductants may be incompatible with some proteins and reagents in the reaction mixture. One of the most effective and convenient methods, particularly for small volumes, is using an oxygen scavenging system composed of 1% β-D-glucose, 25 units/ml glucose oxidase, and 250 units/ml catalase. For a review of different methods see attached paper.

  Biochemistry without Oxygen.pdf ×

  Aug 15, 2012
• 
  Praveen Kumar S K · Gulbarga University

  Thanks for ur suggestions but can I create such conditions in a small box etc as such

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  Thanks for ur suggestions but can I create such conditions in a small box etc as such

  Aug 20, 2012
• 
  Tomáš Hluska · Palacký University of Olomouc

  You need to manipulate with the samples somehow in the anaerobic conditions, right? For that you need the hood. Or you could prepare your samples outside and then just put them into small box and with few vacuum-nitrogen cycles you could remove the oxygen from your samples. But I'm not sure, how efficient would that be.

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  You need to manipulate with the samples somehow in the anaerobic conditions, right? For that you need the hood.
  Or you could prepare your samples outside and then just put them into small box and with few vacuum-nitrogen cycles you could remove the oxygen from your samples. But I'm not sure, how efficient would that be.

  Aug 21, 2012
• 
  Munusamy Vivekanandan · Bharathidasan University

  Strict anaerobic condition for protein reconstitution assay becomes difficult but nevertheless impossible.nitrogen flushing was the one used in our times. Glucose oxidase coupled with catalase can be no doubt used in the system but these additions might cause spectrophotometric absorption problems in a mitochondrial protein reconstitution study

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  Strict anaerobic condition for protein reconstitution assay becomes difficult but nevertheless impossible.nitrogen flushing was the one used in our times. Glucose oxidase coupled with catalase can be no doubt used in the system but these additions might cause spectrophotometric absorption problems in a mitochondrial protein reconstitution study

  Sep 14, 2012