How to do pI3 kinase assay by using PI3 Kinase Activity/Inhibitor Assay Kit (millipore)?

I want to do PI3 Kinase Activity assay by using millipore PI3 Kinase Activity/Inhibitor ELISA kit in that protocol they didn't mention the details about the sample volume in the the Kinase/Inhibitor Reaction setup. How much concentration (volume) of sample should I use?