# How to denote letters to mark significant differences in a bar chart plot

need your help to find a solution for my problem to indicate significant differences in a bar chart plot. The data of the statistical test is available in the following format:

Bar1-bar2:significant

Bar1-bar3:not significant

I want to mark significant differences between two bars with different letters (like bar1:a and bar2:b). If there is no significant differences between two bars they get the same letter (like bar1:a and bar3:a). Sort the right letters to the bars gets much more complex when the number of bars increases. I have several hundreds of statistical comparisons here and have to find a computer program that can generate the letters from the data for me.

Anybody an idea which programme can help me?

I have added an example plot with letter-coded significant differences to illustrate what I want to do.

*** If any one can help me to obtain a good reference material that guide to Interpretation and analysis of biological research data would be much grateful.

Attached a sample e.g.

Bar1-bar2:significant

Bar1-bar3:not significant

I want to mark significant differences between two bars with different letters (like bar1:a and bar2:b). If there is no significant differences between two bars they get the same letter (like bar1:a and bar3:a). Sort the right letters to the bars gets much more complex when the number of bars increases. I have several hundreds of statistical comparisons here and have to find a computer program that can generate the letters from the data for me.

Anybody an idea which programme can help me?

I have added an example plot with letter-coded significant differences to illustrate what I want to do.

*** If any one can help me to obtain a good reference material that guide to Interpretation and analysis of biological research data would be much grateful.

Attached a sample e.g.

## All Answers (6)