How does RNA and Plasmid DNA get dissolved in Isopropanol step of DNA isolation? - ResearchGate
How does RNA and Plasmid DNA get dissolved in Isopropanol step of DNA isolation?
What makes Isopropanol eat away all the RNA and Plasmid in DNA isolation?
Genomic DNA Isolation
Plasmid DNA Isolation
Topic pending review
Apr 4, 2013
· Francis Crick Institute
I'm a little unclear as to what you're asking. What kind of DNA isolation are you doing? However, in DNA isolation steps that I'm familiar with, the plasmid DNA does not get dissolved in isopropanol - it precipitates! You add the isopropanol (or sometimes ethanol) for the purpose of precipitating the DNA. As to why the RNA doesn't get precipitated by the isopropanol as well, in the DNA isolation kits I use that is because it is digested with RNases present in the buffer used to resuspend the bacterial pellet.
Apr 8, 2013
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Francis Crick Institute
Spanish National Research Council
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