Question
How does increased NaCl concentration in culture broth effect the growth Vibrio Cholera?
Gram negative bacterial culture.
All Answers (22)
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Increasing concentration of NaCl will have a negative impact on the growth of V. cholerae, which grow well in the range 0.5% to 0.85% salt. -
thank you so much :) :D -
Concentration of NaCl range must be 0.5% to 0.85% for growing V.chlorae. -
Higher salt concentrations will slow down growth, but certainly not inactivate the organisms. If conditions become more favourable (lowering of salt concentration), they will come back -
Viibrio cholerae and Vibrio mimicus can grow in peptone water meduims without added NaCl. Can grow on CLED - Cysteine Lactose Electrolyte deficien medium that used for urine culture. . They are called non halophillic vibrios. They will also grow in 0.5, 1, 3, 6% . Higher concentration will inhibit growth. -
Does any osmotic pressure affects the growth either by modifying energy metabolic activity or membrane biosynthetic steps? -
Thank you all of you :)
I have culture them at 0% , 6% and 8% .. Thank you Karven :) -
Although the growth is slower, we have assayed NaCl concentrations between 0.5M and 1M, and V. cholerae grows with lower specific growht rate at those high salt concentrations, although you can obtain high viabilities values at the end of culture. Finally it will depends on the strain, if you are working with mutants salt tolerants, you must expect the cells are more adapted to salt and cosenquently the growth will be better in comparison to wild type strains. -
I have cultured it in 1% with slow growth rate. -
Vibrios their growth stimulated by addition of NaCl.
Unidentified vibrios have been called “marine species,” or simply, “marine vibrios.” These marine species are defined as Vibrio or Photobacterium strains that are do not grow in nutrient broth without added NaCl.
V. cholerae able to grow extensively in different kinds of freshwater. The bacterium multiplied in river water, lake water and effluent of a wastewater treatment plant.
Growth characterized in lake water samples amended with different concentrations of NaCl. The highest micromax of V. cholerae was recorded at moderate salinity levels (5 g NaCl l(-1), micromax=0.84 h(-1)), whereas at 30 g NaCl l(-1) (micromax=0.30 h(-1)) or 0 g NaCl l(-1) (micromax)=0.40 h(-1)) specific growth rates were significantly reduced. These results demonstrate that V. cholerae is not only able to survive, but also able to grow in freshwater samples. -
Vibrio cholerae is both an intestinal pathogen and a microbe in the estuarine community. To persist in the estuarine environment, V. cholerae must adjust to changes in ionic composition and osmolarity. These changes in the aquatic environment have been correlated with cholera epidemics. the response of V. cholerae to increases in environmental osmolarity. Optimal growth of V. cholerae in minimal medium requires supplementation with 200 mM NaCl and KCl. However, when the NaCl concentration is increased beyond 200 mM, a proportionate delay in growth is observed. During this delay in growth, osmotic equilibrium is reached by cytoplasmic accumulation of small, uncharged solutes that are compatible with growth. We show that synthesis of the compatible solute ectoine and transport of the compatible solute glycine betaine impact the length of the osmoadaptive growth delay. We also demonstrate that high-osmolarity-adapted V. cholerae displays a growth advantage when competed against unadapted cells in high-osmolarity medium. In contrast, low-osmolarity-adapted V. cholerae displays no growth advantage when competed against high-osmolarity-adapted cells in low-osmolarity medium. These results may have implications for V. cholerae population dynamics when seawater and freshwater and their attendant microbes mix. -
It will help you.... -
Thank you :) :D
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The Vibrio Cholera should be cultured from low NaCl concentration to high NaCl concentration.
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There is an optimal NaCl requirements for Vibrio cholera, or dependent on the strains that you are to cultivate. I would suggest that you do range finding tests to be able to get the best result at a certain concentration. -
V cholerae grows well in different salt conditions is a welladapted halotolerant and halophilic bacteria. So, it is necessary to determine the optimal range of salinity for growing. -
As Vibrio Cholera is facultatively anaerobic,it requires NaCl for its growth.Some species of Vibrio do not grow without adding NaCl,but do grow in Nutrient Broth by adding NaCl.The increased salinity i.e the increased NaCl conc. influences the growth of Vibrio.Studies on the effect of selected ions on growth and activity of v.cholerae demonstrated that Na+ was required for growth.so I think increased NaCl conc. will enhance the growth of V.cholerae. -
due to salinity the bacterial growth get affected with this but the salinity helpful in the case of fungus. and in a research its proved also that eukaryotic organism shows better growth in saline and prokaryotic shows less growth. so in ur case the vibrio is a prokaryotic organism and it require a less salt content or no salt content. -
You should grow vibrio in less saline water and then higher amount of salt.it will effect to growth of vibrio.and get better result to do like this -
As u r asking about special Vc.definitely it will grow even in the Ph 10.2.You can use alkaline Pep-tone water for this Growth.If you want more information Go to USFDA-BAM web site.I think it will help you. -
Dear Pandey,
The V.Cholera is a salt loving microbe. If salinity is decreased, the optimum levels of growth will decreased.
Maxmimum the Vibrio family are salt lovers. So obviously, the growth will decreased. -
Vibrios are salt tolerant, but vibrio cholerae and V. fluvialis can grow in 0% of salt
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