for plasmid/shRNA transfection of HEK 293 cells I advise a reagent called Viromer RED/YELLOW which was developed by a company called Lipocalyx:
For transfection of siRNA/miRNA please refer to Viromer BLUE/GREEN:
If you have further questions please feel free to contact me: Olivia.Zabel@lipocalyx.de
If you are still working on this, we have a protocol that is working really well, involving a new transfection reagent I can share with you if helpful.
I have to go for a double transfection with 293 cells.Any thoughts in regards to calcium phosphate method ?
And which controls i must add ?
Aníbal I. Acuña
Austral University of Chile
Hospital de la Santa Creu i Sant Pau
Mechnikov Research Institute of Vaccines and Sera
University of Florida
Middle East Technical University
Royal Veterinary College