Gene silencing in HUVECs

All Answers (6) Show full discussion

• 
  Nihar R. Pandey · University of Ottawa

  Here is a protocol for siRNA Transfection using Thermo Scientifi c DharmaFECT® 1 in HUVEC ..... https://www.thermo.com/eThermo/CMA/PDFs/Various/File_7311.pdf Try to optimize your transfection condition accordingly. Alternatively, you may try FuGENE® 6 or FuGENE® HD transfection reagents (protocol optimization is recommended).

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  Here is a protocol for siRNA Transfection using Thermo Scientifi c DharmaFECT® 1 in HUVEC .....
  https://www.thermo.com/eThermo/CMA/PDFs/Various/File_7311.pdf
  
  Try to optimize your transfection condition accordingly. Alternatively, you may try FuGENE® 6 or FuGENE® HD transfection reagents (protocol optimization is recommended).

  Jul 10, 2012
• 
  Shuyong Zhu · Institute Of Neuroscience

  I would like to try the electroporation technology, such as Amaxa Nucleofector , which could give rise to high transfection efficiency

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  I would like to try the electroporation technology, such as Amaxa Nucleofector , which could give rise to high transfection efficiency

  Jul 11, 2012
• 
  Paula Saavedra García · Universitat Rovira i Virgili

  Thank you very much for your answers =D

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  Thank you very much for your answers =D

  Jul 11, 2012
• 
  Emmanuel Di Valentin · University of Liège

  We use HIV-1 derived viral vectors (VSV-G pseudotyped) to introduce shRNA sequence into Huvec.

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  We use HIV-1 derived viral vectors (VSV-G pseudotyped) to introduce shRNA sequence into Huvec.

  Jul 12, 2012
• 
  Gary Davidson · Karlsruhe Institute of Technology

  Try ScreenFect A (www.incella. com) It works really well for siRNA transfection

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  Try ScreenFect A (www.incella. com)
  It works really well for siRNA transfection

  Jul 12, 2012
• 
  Leonardo Chicaybam · Brazilian National Cancer Institute

  If you have access to Nucleofector system, this is the simplest and cheapest way: http://www.sciencedirect.com/science/article/pii/S0003269708008464

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  If you have access to Nucleofector system, this is the simplest and cheapest way: http://www.sciencedirect.com/science/article/pii/S0003269708008464

  Jul 13, 2012