Do you apply Liquid Nitrogen at begining step of DNA isolation? We are now facing problems. The liquid nitrogen makes dificulty grounding mycelium to powder, it is stack. Even mycelium was dried by soft tissue after picked up from liquid culturing.
Any suggetions are highly appreciated.
we have found that bead beating with lysis buffer (any available or home made) was effective for any downstream applications such as qPCR and NGS.
we developed a quick method (using microwave) that works great for sequencing in Fusaria and should easily apply also to Aspergillus. if you wish to give a try you found it here
Hello Tran Ho Quang: If using a mortar and pestle, try to form a thin layer of mycelium and if it has sticking to the bottom of the mortar, unstick it before adding nitrogen (you can do with a spatula).
University of Münster
Ben-Gurion University of the Negev
University of Texas at El Paso
Seyyed Mehdi Mousavi
University of Applied Science and Technology, Guilan , Iran
Children's Mercy Hospitals and Clinics
Saint Joseph University, Lebanon
University of Carthage
MD IMRAN KHAN