Currently I am trying to make multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) liver tissue. Unfortunately, tons of red blood cells with strong autofluorescence at 488 and 546 excitation were found in tissue sections, though I have made blocking procedure using 5% BSA before antibody staining.
Does anyone have any suggestions to reduce or eliminate autofluorescence by red blood cells? May cryosections help? Thank you very much in advance!