I thank you all for helping me design my cell viability assay with much more criteria. I will be searching for good references to strengthen your valuable tips for this experiment
It all depends on the cell line you are using. I have tested DMSO toxicity on three prostate cancer cell lines, one cell line shows a statistically significant effect at >=0.5% while the other two are statistically significant at >=1%. You could design an assay using varying concentrations of DMSO to determine a threshold level for your extracts. Good luck :-)
This page might help you :
Typically DMSO <0.01% final concentration should not be toxic to cells. I have worked with 0.01% DMSO and did not find it cytotoxic to T-cells.
Thank you all for the answers !
I have been working with HaCat cells using a final concentration between 0,01 - 0,015% and the cells weren´t affected. Very precious tip about paying attention to the cell line you are working with !
Best regards and good luck to all :)
Just to let you know that I have used 0.1% - 0.15% of DMSO and the HaCat cells behaved well. For sure that I have prepared my DMSO control simultaneously.
Best regards to all
University of the West of England, Bristol
University of Alberta
National University of Malaysia
Kris Cahyo Mulyatno
Institute of Technology, Tralee
Medical College of Wisconsin
University of Lucknow