Does TE buffer lyse human white blood cells?
In an experiment, I prepared serial dilutions from fresh human blood samples (EDTA tubes) using TE buffer. Now I need to know if the white blood cells were intact or not in the diluted blood (there are no samples available today). The TE buffer used was made by mixing 10mL Tris-HCl (1M pH 8.0) and 0.2mL EDTA (0.5M) in 990mL Milli-Q water. Any suggestions or references would be appreciated.