Culture media for breast cancer cell lines?
The literature is full of different "recipes" for each of the above-mentioned cell lines and I was wondering which is the one that is most commonly accepted/used. For example, I have read that some people growing MCF-7 cells add insulin to the media, others don't. And there are plenty of these examples. Any help from people who have worked with these cell lines before?
And in addition, if you could share information about doubling times, split rations and any useful tips I would be grateful.