Conventional DC preparation from Human Monocytes and co-culture with autologous T-cells.
I am preparing DC from Human Monocytes which I isolated from PBMC (using ficoll gradient) using Miltiyeni CD14 micro-beads isolation. Then I culture them in 6 well plate in presence of GmCSF n IL-4 50ng/ml. After 7 days I have to co-culture these DC with autologous T cells. Now the problem I am facing is how to detach these adhered DCs? What amount and when should I add IL-2 after co-culturing them with autologous T-cells?