Cell printing

All Answers (4) Show full discussion

• 
  Brent Puthoff · University of North Carolina at Chapel Hill

  What type of device are you using to do the printing?

  Cancel Save

  What type of device are you using to do the printing?

  Jun 13, 2012
• 
  Yasuyuki Sakai · The University of Tokyo

  Many studies are focused on how to build tissue, but to get stably functional tissues we need to pay special attentons to oxygen/nutrient supply to cells in hydrogels, that is, covnection/perfusion in the gaps or flow channels or difussion in hydrogel. The situation strongly depend on the demand of cells and cell density in hydrogel.

  Cancel Save

  Many studies are focused on how to build tissue, but to get stably functional tissues we need to pay special attentons to oxygen/nutrient supply to cells in hydrogels, that is, covnection/perfusion in the gaps or flow channels or difussion in hydrogel. The situation strongly depend on the demand of cells and cell density in hydrogel.

  Jun 13, 2012
• 
  Deleted

  As Brent was correctly addressing, the type of technology used for the printing process influence drastically the criteria you have to take care. In case of bioprinting, an approach to construct a biological structure by computer-aided, automatic, layer-by-layer deposition of small amounts of biological material that should permit the prouction of complex living and non-living products by placing specifically protein, DNA, cells, hormones, and ECM within the desired structure. A “bioink” (cell aggregates or spheroids) is deposited together with a biopaper (scaffold material) into predesigned 3D organizations. For bioprinting essentially three criteria have to be met to successfully function as bioprinting material: - The material should be mechanically suitable for printing: - The material should maintain its structural integrity after deposition process - The material should provide a cytocompatible environment before, during and after the deposition process. The success of bioprinting so far has been limited by a paucity of biomaterial that are compatible with printing devices, having the optimal balance of robustness, extrudability, cytocompatibility, and biodegradability. To develop a polymer along with processing conditions should be appropriate for both accurate printing and cell culture and often, these criteria impose opposing requirements. here you can find a nice overview of the challenges of the Cell printing technology. http://www.sciencedirect.com/science/article/pii/S0079670011001328 or here www.mdpi.com/2079-4983/2/3/119/pdf

  Cancel Save

  As Brent was correctly addressing, the type of technology used for the printing process influence drastically the criteria you have to take care.
  
  In case of bioprinting, an approach to construct a biological structure by computer-aided, automatic, layer-by-layer deposition of small amounts of biological material that should permit the prouction of complex living and non-living products by placing specifically protein, DNA, cells, hormones, and ECM within the desired structure. A “bioink” (cell aggregates or spheroids) is deposited together with a biopaper (scaffold material) into predesigned 3D organizations.
  
  For bioprinting essentially three criteria have to be met to successfully function as bioprinting material:
  - The material should be mechanically suitable for printing:
  - The material should maintain its structural integrity after deposition process
  - The material should provide a cytocompatible environment before, during and after the deposition process.
  The success of bioprinting so far has been limited by a paucity of biomaterial that are compatible with printing devices, having the optimal balance of robustness, extrudability, cytocompatibility, and biodegradability. To develop a polymer along with processing conditions should be appropriate for both accurate printing and cell culture and often, these criteria impose opposing requirements.
  
  here you can find a nice overview of the challenges of the Cell printing technology.
  
  http://www.sciencedirect.com/science/article/pii/S0079670011001328
  or here
  
  www.mdpi.com/2079-4983/2/3/119/pdf

  Jun 18, 2012
• 
  Krishna Kommareddy · Max-Planck-Institut für Kolloid- und Grenzflächenforschung

  Thank you all for your input. Its really informative and invaluable. At the moment I am working on photo-polymerizing acrylate based material using stereolithography technique. In future I would like to venture the field of 3D organ printing. Thank you Barry for the citation. It is helpful in understanding the current challenges and available technology.

  Cancel Save

  Thank you all for your input. Its really informative and invaluable. At the moment I am working on photo-polymerizing acrylate based material using stereolithography technique. In future I would like to venture the field of 3D organ printing. Thank you Barry for the citation. It is helpful in understanding the current challenges and available technology.

  Jun 20, 2012