Question
Can cancer cell lines be cancerous or infectious inside injured body parts of the user ?
Handling of cancer cell lines.
All Answers (22)
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No...if you have a healthy immune system ;) -
Yes :s by definition....
A cancer cell is "a cell able to form a tumor if implanted in a sane organism" so... of course your immune system may help you but you should be carefull... the question is, how could such a thing happened?
don't you use gloves? labcoat? shouldn't you handel them in steril condition? -
no i am using all the precautions Mr. Benjamin Leduc ·, but if in case accidentally happened, for that i asked this question. thanks . -
Can be though it depends of lots of factors such kind of cancer cell, type of exposed tissue, local immune system and microenvironment. After all, I must confess that homing and propagation, perhaps spreading, of such cells in healthy people is very rare ( very low efficacy). -
I disagree...
a "foreign" tumor cell, if your immune system works, is eliminated by the organism...
This is the reason why usually you have to use immuno-compromised animals in order to form tumor.... -
Should maybe experiment it.... take some of your cells, inject it in an healthy unrelated organism, a mouse for exemple, and... normally a tumor should devlop...
Anyway, I cannot imagin that any scientist could work with an open injury, trow on it cancer cells, without washing it and let cells come in.... -
Again...I disagree... Indeed, we have many problems with tumor cell injections...it's very difficult to produce xenografts in mice immuno-compromised...It would be "quite" impossible to produce tumor in Humans... (i.e. GVHD...)
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Clearly, in principle it is possible. However, I think it is quite unlikely as it would depend on the type of cancer cells, type of tissue injected, immune system and many other things.... -
Immune cell are infiltrated at the site of injury and its seems to me impossible for the tumor cell will grow at that site...Unless it’s immunodeficient chances are there but still tissue specific immune cell will secrete cancer inhibitory components.
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I disagree. even in immuno compromised mice, you need a particular number of cells to induce tumor. In such case, highly immune cell infiltrated injured region will give absolute no to tumor induction, Unless you purposely inject the cells. -
No, never! There are NO reported cases of any "infection" by cultured tumour cells.
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>Can cancer cell lines be cancerous or infectious inside injured body parts of the user ?
Cancer due to culture cells?
No ... except if you immortalized your own cells ... which is forbidden in most countries.
Infection due to culture cells?
Yes in theory. It depends if the cell line contains a virus.
Keep this rule in mind :
ALL HUMAN SAMPLE HAVE TO CONSIDERED AS POTENTIALLY INFECTIOUS. -
There is almost no chance that any direct infection or transmission of cancer can occur to a lab worker. However, there is a chance that any adventitial or cancer-associated microorganisms present in the cell culture can be transmitted to the user. This includes viruses like Epstein-Barr and erythroleukemia as well as other cancer-associated retroviruses. Prions have also been found closely associated with some types of cancer (e.g., pancreatic tumors) but no causal connection is known. Transmission of cancer to a human host from these sources would be highly unusual and may not even occur but prudence in handling such materials is wise.
Aside from these unlikely possibilities, the most likely sources of risk to users of tumor lines are pathogens like HIV, TB, and hepatitis carried by the cell line. This is the main risk when using primary cells obtained from humans, as when attempting to derive new cancer cell lines from tumor samples or when using any cells or tissues derived from human subjects. For this reason, it is important to be aware of the origin of human cell lines being used in the lab and to handle them with care and at least biosafety level 2 procedures. Many, but not all, cell lines obtained from reputable sources like ATCC have been screened for pathogens and this information is available to users. If specific information is not available, then cells, used labware, and spent media should be treated as infectious and should be decontaminated before disposal. -
An example of infection here:
http://www.ncbi.nlm.nih.gov/pubmed?term=7603227 -
And never give the shield on a used needle !!!
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Tasmanian devil actually disappear due to the propagation of cancer cells after brawls and devil bites. It is because they are genetically too much similar (so the immune system cannot eliminate the cancer cells).
In theory - even if it is very rare - we cannot rule out that two humans can share strong genetic similarities. In conclusion: just be careful to everything especially with human samples. -
Read this very good protocol review entitled "Changing medium and passaging cell lines":
http://www.ncbi.nlm.nih.gov/pubmed?term=17853884 -
"Accidental inoculation of a cancer cell line in a needle led to the growth of a tumor (ref. 4) and it is well known that cancers can be transferred during transplantation (e.g., ref. 3). The risk is liable to be greater for individuals who are immunodeficient, and therefore such persons should consider carefully and in consultation with their occupational health department whether they should be working with cell lines and, if so, what extra precautions might be taken."
http://www.ncbi.nlm.nih.gov/pubmed?term=17853884
That's it! -
Needle-stick TRANSMISSION of human colonic ADENOCARCINOMA.
Gugel EA, Sanders ME.
N Engl J Med. 1986 Dec 4
volume 315 - n°23 - page 1487.
http://www.ncbi.nlm.nih.gov/pubmed?term=3785302
Never say never! -
Infection of laboratory workers with hantavirus acquired from immunocytomas propagated in laboratory rats.
Lloyd G, Jones N.
J Infect. 1986 Mar
volume 12 - n°2 - pages 117-125.
http://www.ncbi.nlm.nih.gov/pubmed?term=2871112 -
This happened for me with mouse melanoma B16F10 during intravenous injection of these cells, I had selected these cells to express RFP, I think I got about 5000 cells (one drop). I hope my immune system works well otherwise I will be a red fluorescent expressing man :)))))) -
>otherwise I will be a red fluorescent expressing man :))))))
After Spiderman (bitten by a spider) ... Mouseman.
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