Behaviour - watermaze and object recognition

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• 
  Christophe Bernard · Aix-Marseille Université

  For an easy test for spatial (hippocampus-dependent) and non spatial memory), you can check Chauviere et al, J Neuroscience, 2009

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  For an easy test for spatial (hippocampus-dependent) and non spatial memory), you can check Chauviere et al, J Neuroscience, 2009

  Aug 7, 2012
• 
  Marcos Malumbres · Centro Nacional de Investigaciones Oncológicas

  For the object recognition test this is the best reference: Bevins, R. A. & Besheer, J. Object recognition in rats and mice: a one-trial nonmatching-to-sample learning task to study ‘recognition memory’. Nature Protoc. 1, 1306–1311 (2006)

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  For the object recognition test this is the best reference: Bevins, R. A. & Besheer, J. Object recognition in rats and mice: a one-trial nonmatching-to-sample learning task to study ‘recognition memory’. Nature Protoc. 1, 1306–1311 (2006)

  Aug 7, 2012
• 
  Deleted

  You have also a good resource in Lausanne: Get into contact with Magara Fulvio.

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  You have also a good resource in Lausanne: Get into contact with Magara Fulvio.

  Aug 7, 2012
• 
  Marwan Azzubaidi · International Islamic University Malaysia

  Recently I have published this article (link below) for long-term, short-term and working memory water maze protocols if you would like to have a look. http://www.ane.pl/showarticle.php?art=7213 you can download and have a detailed look at the different protocols I have employed.

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  Recently I have published this article (link below) for long-term, short-term and working memory water maze protocols if you would like to have a look.
  http://www.ane.pl/showarticle.php?art=7213
  you can download and have a detailed look at the different protocols I have employed.

  Aug 7, 2012
• 
  Samia Habbas · Université de Lausanne

  Thanks a lot to everyone for your help!

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  Thanks a lot to everyone for your help!

  Aug 7, 2012
• 
  Jasbeer Dhawan · Stony Brook University

  For NORT look my paper •Dhawan J., Benveniste, H., Luo, Z., Nawrocky, M., Smith, SD, and Biegon A. A new look at glutamate and ischemia: NMDA agonist improves long-term functional outcome in rat model of stroke. Future Neurology, 6:823-834 (2011). If you have any questions you can ask me

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  For NORT look my paper •Dhawan J., Benveniste, H., Luo, Z., Nawrocky, M., Smith, SD, and Biegon A. A new look at glutamate and ischemia: NMDA agonist improves long-term functional outcome in rat model of stroke. Future Neurology, 6:823-834 (2011).
  If you have any questions you can ask me

  Aug 7, 2012
• 
  Deveroux Ferguson · Mount Sinai School of Medicine

  Hi Samia, for an excellent methodological description of object recognition task. Please feel free to download my paper Overexpression of mineralocorticoid and transdominant glucocorticoid receptor blocks the impairing effects of glucocorticoids on memory. Ferguson D, Sapolsky R. Hippocampus. 2008;18(11):1103-11. and Mineralocorticoid receptor overexpression differentially modulates specific phases of spatial and nonspatial memory. Ferguson D, Sapolsky R. J Neurosci. 2007 Jul 25;27(30):8046-52

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  Hi Samia, for an excellent methodological description of object recognition task. Please feel free to download my paper
  Overexpression of mineralocorticoid and transdominant glucocorticoid receptor blocks the impairing effects of glucocorticoids on memory. Ferguson D, Sapolsky R. Hippocampus. 2008;18(11):1103-11.
  
  
  and
  
  Mineralocorticoid receptor overexpression differentially modulates specific phases of spatial and nonspatial memory.
  Ferguson D, Sapolsky R.
  J Neurosci. 2007 Jul 25;27(30):8046-52

  Aug 13, 2012
• 
  Jennie Young · Massachusetts Institute of Technology

  We routinely use the Morris water maze (MWM) in our lab as an assay of spatial memory that is acquired over many repeated trials. I agree with CHohmann that MWM can be difficult to carry out in mice. However, we have successfully tested multiple lines of transgenics without problems (Nakazawa et al., Science, 2002; Nakashiba et al., Science, 2008; Suh et al., Science, 2011). Variations such as the delayed-match-to-sample MWM may also be run to test one-trial memory (Nakazawa et al., Cell, 2003). We have also successfully tried novel object recognition (NOR) in mice as a one-trial memory task. However, the literature on the hippocampus-dependent nature of NOR based on lesion/inactivation studies is mixed. [This is not to say that information tied to object identity is not processed by the hippocampus. Rather, recognition of the object may be done independently of the hippocampus, depending on the task conditions.] Instead, the hippocampus has been more strongly implicated in paired associates of sensory information, such as object + place. Therefore, our lab uses a related behavioural assay, novel location recognition (NLR), to examine object-place associations as a more reliable test of hippocampal function. For an excellent overview of NOR, NLR, and other variations, please see Langston et al., Behav Brain Res., 2010. Please let me know if you would like more specifics on how we run our behavioural protocols.

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  We routinely use the Morris water maze (MWM) in our lab as an assay of spatial memory that is acquired over many repeated trials. I agree with CHohmann that MWM can be difficult to carry out in mice. However, we have successfully tested multiple lines of transgenics without problems (Nakazawa et al., Science, 2002; Nakashiba et al., Science, 2008; Suh et al., Science, 2011). Variations such as the delayed-match-to-sample MWM may also be run to test one-trial memory (Nakazawa et al., Cell, 2003).
  
  We have also successfully tried novel object recognition (NOR) in mice as a one-trial memory task. However, the literature on the hippocampus-dependent nature of NOR based on lesion/inactivation studies is mixed. [This is not to say that information tied to object identity is not processed by the hippocampus. Rather, recognition of the object may be done independently of the hippocampus, depending on the task conditions.] Instead, the hippocampus has been more strongly implicated in paired associates of sensory information, such as object + place. Therefore, our lab uses a related behavioural assay, novel location recognition (NLR), to examine object-place associations as a more reliable test of hippocampal function.
  
  For an excellent overview of NOR, NLR, and other variations, please see Langston et al., Behav Brain Res., 2010. Please let me know if you would like more specifics on how we run our behavioural protocols.

  Aug 17, 2012