Biotechnology and Bioprocess Engineering

Publisher: Springer Verlag

Current impact factor: 1.11

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 1.113
2013 Impact Factor 1.22
2012 Impact Factor 1.277
2011 Impact Factor 1.278
2010 Impact Factor 1.004
2009 Impact Factor 1.412
2008 Impact Factor 1.653
2006 Impact Factor 1.366
2005 Impact Factor 1.349

Impact factor over time

Impact factor

Additional details

5-year impact 1.25
Cited half-life 5.10
Immediacy index 0.16
Eigenfactor 0.00
Article influence 0.26
ISSN 1976-3816
OCLC 220882822
Material type Series, Periodical
Document type Journal / Magazine / Newspaper

Publisher details

Springer Verlag

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Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The potential of isolated actinomycetes and fungi were evaluated for the cellulase and xylanase production under solid state fermentation conditions. Maximal secretion of enzymes was observed with Phanerochaete chrysosporium using soybean straw. The potential of the produced crude enzyme complex was demonstrated by two-step enzymatic hydrolysis of untreated and mild acidpretreated sorghum husk (SH). A cellulase dose of 10 filter paper units (FPU) released 563.21 mg of reducing sugar (RS) per gram of SH with 84.45% hydrolysis and 53.64% glucose yields, respectively. Finally, enzymatic hydrolysates of SH were utilized for hydrogen production by Clostridium beijerinckii. Effects of temperature, pH of media, and substrate concentration on the biohydrogen production from SH hydrolysates were investigated. The optimal conditions for maximal hydrogen production using SH hydrolysate were determined to be a loading of 5.0 g RS/L, at 35°C, and controlled pH at 5.5. Under these optimal conditions, the cumulative H2 production, H2 production rate, and H2 yield were 1,117 mL/L, 46.54 mL/L/h, and 1.051 mol/mol RS, respectively. These results demonstrated a cost-effective hydrogen production is possible with sorghum husk as a lignocellulosic feedstock.
    Biotechnology and Bioprocess Engineering 08/2015;
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    ABSTRACT: Understanding aerobic denitrification has become an important focus of environmental microbiology. Aerobic denitrification can be performed by various genera of microorganisms and describes the use of nitrate (NO3-) as oxidizing agents under an aerobic atmosphere. Isolation of aerobic denitrifiers, enzymes involved in aerobic denitrifiers, phylogenetic distribution of aerobic denitrifiers, factors affecting the performance of aerobic denitrifiers, attempts of applications and possible future trends are depicted. The periplasmic nitrate reductase is vital for aerobic denitrifiers and NapA gene may be the proof of aerobic denitrification. Phylogenetic analysis revealed that aerobic denitrifiers mainly belong to α-, β- and γ-Proteobacteria. Aerobic denitrifiers tend to work efficiently at 25 ~ 37°C and pH 7 ~ 8, when dissolved oxygen concentration is 3 ~ 5 mg/L and C/N load ratio is 5 ~ 10. In addition, recent progresses and applications on aerobic denitrifiers are described, including single aerobic reactors, sequencing batch reactor and biofilm reactors. The review attempts to shed light on the fundamental understanding in aerobic denitrification. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):643-651. DOI:10.1007/s12257-015-0009-0
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    ABSTRACT: Secretion of homologous proteins in large amounts has been accomplished for many proteins, but no efficient secretion system has been described so far which can be generally applied for heterologous proteins. The objective of this review article is to compare the three major secretion pathways in E. coli and in B. subtilis and review the stages of conversion of the secreted proteins from the unfolded polypeptide chains into the correctly folded and fully active protein. Furthermore, bottlenecks in the production of heterologous proteins and the ways to resolve them are briefly discussed. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):623-633. DOI:10.1007/s12257-014-0843-5
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    ABSTRACT: In the present study, we investigated the effects of multi-herbal water extract mixture, Taeumjowi-tang (TH) on liver proteome alteration in mice using twodimensional electrophoresis combined with MALDI-TOFMS. Animals were fed high-fat diet with or without TH (0.3% wt/wt) supplement for 12 weeks. At the end of 5th week of experimental diet, mice fed high-fat diet only were subdivided into 2 groups, obesity-prone (OP) and obesityresistant (OR) mice based on weight gain. OR mice gained less body weight compared to OP mice despite of same food intake. TH significantly suppressed weight gain, and proteomic analysis enabled the identification of 49 liver proteins showing differential regulation between OP and OR/TH mice. Combined results of proteomic and western blot analyses revealed decreased lipogenesis via three fatty acid metabolic targets (AMPK, ACC, and FAS) in livers of OR and TH mice. Using bioinformatic classification and network analysis, most of the identified proteins were classified as hydrolases, oxidoreductases, transferases, defense/immunity proteins, and enzyme modulators based on functional analysis of the PANTHER classification system. Combined results of proteomic and bioinformatic analyses using GeneMANIA identified two proteins (LACTB2 and NIT2) in the liver that potentially interact with fatty acid metabolic proteins. Furthermore, these proteins were included in acetylation, phosphoprotein, and metabolic processes in DAVID classification. These proteins were highly expressed in OP mice; however both their transcription and protein expression were lowered by TH treatment. In conclusion, combined data from proteomic and network analyses suggest that TH exerts anti-obesity effects by modulating fatty acid metabolic proteins/genes, particularly via the AMPK pathway. Most targeted proteins/ genes were modulated toward enhancing lipid metabolism in response to TH treatment. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):775-793. DOI:10.1007/s12257-015-0258-y
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    ABSTRACT: Glucagon-like peptide-1 (GLP-1) was a potential therapeutic drug for type II diabetes, mainly because of the stimulatory effect on insulin secretion under condition of high blood glucose. We used PCR to obtain a recombination gene, GGH, in which two GLP-1 (GLP-1A2G) mutants were connected in series and then fused to the N terminal of human serum albumin. The fusion gene was inserted into pGAPZaA plasmid with Saccharomyces cerevisiae α-factor secretion signal sequence, and was expressed by the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The engineered strain was constructed by integrating the recombinant plasmid pGAPZαA/GGH into the genome of Pichia pastoris GS115. Genome PCR and western blot showed that the recombinant P. pastoris successfully expressed the fusion protein GGH. The yield of GGH reached 78 mg/L after 72 h fermentation in a flask, using glucose as the optimal carbon source. Fed-batch fermentation was investigated in a 5 L bioreactor, and the expression level of GGH reached 246 mg/L in 52 h. The fusion protein GGH was purified in four steps, and the final purity was 96.1%. The in vitro bioactivity of GGH was the same as that expressed in P. pastoris by the AOX1 promoter. This study described an efficient way to express GGH fusion protein in P. pastoris using GAP promoter, fermentation was easier to control without carbon source change and fermentation time was 20 h less than AOX1 promotercontrolled GGH fermentation. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):694-700. DOI:10.1007/s12257-014-0818-6
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    ABSTRACT: Schisandra chinensis has been used as traditional medicine. The structures of isolate active compounds (schisandrin B, deoxyschisandrin, schisandrin C) from S. chinensis were characterized by physical and spectroscopic analyses. Active compounds were tested for their potential to act as anti-melanogenesis or skin-whitening agents by their abilities to inhibit tyrosinase activity in the cell-free mushroom tyrosinase assay and cellular tyrosinase derived from B16 melanoma cells. The tyrosinase inhibitory activity was correlated to the inhibition of melanin productions in a-MSH-stimulated and unstimulated B16 cells. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver- Burk plot. Schisandrin B was minimally cytotoxic (cell viability: 88.99% at 0.75 µM) and the IC50 value for suppression of mushroom tyrosinase activity was estimated as 0.6 µM. Zymography analysis demonstrated schisandrin B’s concentration-dependent effects and the kinetic analysis indicated schisandrin B’s noncompetitive-inhibitory action. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):814-823. DOI:10.1007/s12257-014-0867-x
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    ABSTRACT: In this study, several chemical treatment techniques commonly used for protein extraction were investigated for recovering glutaryl-7-aminocephalosporanic acid acylase (GLA) from recombinant E. coli cells. The best results were obtained by the combined use of cetyltrimethylammonium bromide (CTAB) and KCl. Subsequently, various extraction conditions, such as cation salt, concentrations of CTAB and KCl, extraction temperature, extraction time, and biomass, were optimized to further enhance the release yield and specific activity of GLA. Our results showed that 110% of GLA was released after treatment with 0.5% CTAB (w/v, %) and 0.3 M KCl at 10°C for 12 h, and its specific activity in this extracting solution was approximately 1.5 times higher as compared to that obtained by sonication. This extraction method could avoid the inactivation of GLA caused by drastic mechanical methods, and also enhance its specific activity for industrial extraction. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):718-724. DOI:10.1007/s12257-013-0607-7
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    ABSTRACT: We prepared and characterized calcium carbonate nanoparticles (CC NPs) that were surface-modified with oleic acid (OA) and phosphatidylcholine (PC) in order to improve their suspension stability in an aqueous solution. The improvement in the suspension stability of CC NPs in an aqueous solution may be helpful to extend their applicability to a wider range of biological applications. The CC NPs were coated with OA by making use of their electrostatic potential and were then decorated with PC. The CC NPs surface-modified with OA and PC were successfully constructed, and the existence of the decorated OA and PC in the surface of the PC-OA-CC NPs was observed via transmission electron microscopy (TEM) and was confirmed by thermo gravimetric analysis (TGA), Xray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR) analyses. The PC-OA-CC NPs floating in an aqueous solution exhibited better stability when compared to non-surface-modified CC NPs. The DLS and TEM results revealed that the degree of size agglomeration for the CC NPs was significantly reduced by the surface modification with OA and PC. The PC-OA-CC NPs showed a very low cytotoxicity at a high concentration in terms of the cell viability of the RAW264.7 cells. Consequentially, the stability in suspension of the CC NPs in an aqueous solution could be effectively improved through surface-modification with OA and PC. PC-OACC NPs could be useful in increasing the range biological applications for CC NPs. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 08/2015; 20(4):794-799. DOI:10.1007/s12257-014-0898-3
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    ABSTRACT: Olfactory receptors (ORs), belonging to the Gprotein coupled receptor (GPCR) family, are very difficult to be overexpressed, purified and reconstituted because of their hydrophobicity and complicated structure. These receptors bind to their specific ligands, thus their specificity is very useful for application as a bioelectronic nose. Furthermore, highly purified and well-reconstituted human olfactory receptor (hOR) can be used in various fields, such as in protein-interaction research, drug screening, and analysis of the hOR structure. In this study, human olfactory receptor, hOR2AG1, was produced with high purity and functionally reconstituted in detergent micelles. The hOR2AG1 was overexpressed in Escherichia coli (E. coli) with glutathione S-transferase (GST) and 6xHis-tag as an inclusion body. The hOR2AG1 fusion protein was solubilized in buffer containing sodium dodecyl sulfate (SDS) and purified using Ni-NTA chromatography. The GST domain was removed using proteolytic cleavage before elution from the column. After purification, the hOR2AG1 was successfully reconstituted using nonionic detergents and methyl-ß-cyclodextrin. Finally highly purified and well-reconstituted hOR was obtained, and its biological characteristics were confirmed by using circular dichroism (CD) spectrum and tryptophan fluorescence assay. These results can be applied to develop protein-based sensing systems including a bioelectronic nose and to analyze the native hOR structure using solid-state NMR, X-ray crystallography, or neutron scattering. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):423-430. DOI:10.1007/s12257-014-0897-4
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    ABSTRACT: Lysine decarboxylase (LDC) exhibits a significant role in cadaverine (1,5-pentanediamine, diaminopentane) production from lysine. In this study, an error-prone PCR and DNA shuffling were performed to improve the activity of LDC from Hafnia alvei AS1.1009 for cadaverine production. A sensitive high-throughput screening strategy based on a pH indicator was established for directed evolution of LDC. Several improved mutants were obtained from directed evolution and LDCV147F/E583G mutant showed highest activity to catalyze lysine to cadaverine. This mutant showed 1.62-fold high LDC activity when compared to wild-type. Further analysis by site-directed mutagenesis reveled that only the mutant E583G was sufficient for higher catalytic activity. Wild type LDC and mutant LDCE583G were purified by an improved method including hydrophobic chromatography. These purified enzymes were characterized and the kinetic parameters were compared between LDCE583G and WT LDC. Vmax of LDCE583G was 1.32-fold higher than that of WT LDC. Use of LDCE583G mutant showed 1.48-fold improved productivity of cadaverine when compared to wild type. The concentration of cadaverine in E. coli JM109/pTrc99a-ldc2-41 was 63.9 g/L with conversion yield of 93.4% during 5 h. These results indicate that the mutation has positive effects on improving LDC activity and a potential candidate for cadaverine production. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):439-446. DOI:10.1007/s12257-014-0690-4
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    ABSTRACT: A multifunctional polymer-drug conjugate candesartan-graft-polyethyleneimine (CD-PEI, CP) containing low molecular weight polyethyleneimine (PEI) and candesartan (CD) conjugated via an amide bond was fabricated as a co-delivery micelle of drug and siRNA for potential lung cancer therapy. Here, CD as an angiotensin II type 1 receptor blocker rich in imidazole and tetrazole rings was utilized to strengthen endosomal buffering capacity of CP and suppress tumor angiogenesis. The selfassembled CP/siRNA complexes exhibited desirable and homogenous particle size, moderate positive charges, and efficient release of drug and siRNA in vitro. In addition, CD and siRNA could readily detached from nanovectors in tumor cells via an amidase-responsive mechanism and they achieved synergistic anti-angiogenesis efficacy by effectively downregulating the expression of vascular endothelial growth factor (VEGF) mRNA and protein via different pathways in vitro. In vivo investigation on nude mice bearing A549 tumor xenografts revealed that CP/siRNA complexes possessed strong antitumor activity. These findings suggested that CP could be an ideal nanovector for simultaneous transfer of drug and siRNA, and a multifunctional CP/siRNA co-delivery system with enhanced endosomal buffering capacity, amidase-responsive drug release and synergistic anti-angiogenesis efficacy might be a new promising strategy for effective lung cancer therapy. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):550-560. DOI:10.1007/s12257-014-0858-y
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    ABSTRACT: Orange juice is a well-accepted fruit juice, and is a natural source of various vitamins, especially vitamin C, as well as sugar, potassium, thiamine, folate, flavonoids and antioxidants. The respective fructose, glucose, and sucrose concentrations were 9.3, 22.9, and 48.1 g/L in the original orange juice used in this study, and 183.4, 170.1, and 142.8 g/L after concentration. Over 97% of the sucrose in the juice was enzymatically converted to glucooligosaccharides upon addition of 3 U/mL dextransucrase, prepared from Leuconostoc mesenteroides 512FMCM, at 16°C. The synthesized oligosaccharides comprised 35.0% of the total saccharides in the concentrated juice and 31.7% in the original juice. The optimum conditions for oligosaccharide synthesis using the concentrated juice were 35.2 × 10−1 U/mL dextransucrase and 1% Ca(OH)2. The calories in the original and modified concentrated orange juices were 325.4 and 246.7 kcal/L, respectively. Compared to the original concentrated juice, the enzyme-modified concentrated juice prevented the formation of 62.7% of the insoluble glucan resulting from addition of mutansucrase, produced by Streptococcus mutans. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):447-452. DOI:10.1007/s12257-014-0741-x
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    ABSTRACT: Three-dimensional (3D) cell culture arrays of melanoma cell spheroids were assembled to evaluate the combined effect of a melanogenesis-targeting drug, N-propionyl-4-cysteaminylphenol (NPrCAP), and heat treatment. An array-like multicellular pattern of mouse melanoma B16F1 cells in a collagen gel was established by magnetic cell labeling using a pin-holder device to exert a magnetic force. The cellular spheroids were exposed to NPrCAP and heat (42°C for 1 h) as a model of anti-cancer treatment. As a result, melanogenesis of B16F1 cells was 29-fold higher in this 3D array than in conventional two-dimensional (2D) monolayer cultures. Because the spheroid size was linearly correlated with the cell number within a spheroid, the antiproliferative effect could be evaluated in a non-destructive manner. Moreover, the half-maximal inhibitory concentration of NPrCAP coupled with heat treatment calculated from the spheroid size was 2-fold higher in the 3D array (0.30mM) than in 2D culture (0.15 mM). These results indicate that spheroid formation decreases the chemosensitivity of cancer cells, and this model would be suitable as a susceptibility assay for melanogenesis-targeting drugs. Therefore, this 3D culture model provides a better screening format to evaluate drug and physical treatments for cancer therapy than 2D formats. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):488-497. DOI:10.1007/s12257-014-0724-y
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    ABSTRACT: Human bone marrow-derived mesenchymal stem cells (hMSCs) are capable of self-renewal and differentiation into various tissue lineages, attracting attention as tools for use in cell therapy. However, hMSCs have very poor proliferative capacity and a short life span in culture. To overcome this problem, we expressed the T antigen of SV40 in hMSCs because it is known to have the ability to elevate the growth rate of various primary animal cells. We obtained several hMSCs lines (hMSCs-T) known for stable expression of T antigen. Cells expressing T antigen proliferated on the monolayer of hMSCs, forming high density foci. hMSCs-T showed changed morphology and improved growth rate and life span, and demonstrated preservation of the potential for differentiation into osteoblasts. In addition, hMSCs-T did not proliferate in soft agar culture, indicating that the cells did not transform into tumor cells. In order to evaluate metabolic change of amino acids in hMSCs-T compared to primary hMSCs, we investigated altered amino acids (AA) with gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode (GC-SIM-MS). A total of 14 AAs were positively measured. Results from the Student’s t-test on the hMSCs group mean of the hMSCs-T group showed significantly elevated levels of glycine, proline, pipecolic acid, aspartic acid, lysine and tryptophan, whereas valine, leucine and isoleucine as branched-chain amino acids (BCAAs), and phenylalanine showed a significant decrease. Altered AAs metabolic pattern in the hMSCs-T may explain the disturbance of AA metabolism related to the expression of SV40 T antigen in hMSCs. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):498-505. DOI:10.1007/s12257-014-0730-0
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    ABSTRACT: To decrease the cost of biodiesel production from microalgae, biodiesel was produced from wet marine microalgae by a one-step direct process. In this process, simultaneous lipid extraction from the wet microalgae and transesterification of the lipid with methanol was conducted. Among the combinations of catalysts and organic extraction solvents that were evaluated, sulfuric acid and chloroform represented the optimum combination. The degree of water content for wet microalgae significantly influenced biodiesel production: in the presence of 348.4% water content in intact wet microalgae, lipid extraction efficiency (LEE) was 73.2% and biodiesel conversion (BC) was 50.5%, but at a lower water content of 185.7%, LEE increased to 84.7% and BC to 69.9%. Increasing the amount of chloroform by 50% relative to the standard amount increased LEE and BC to 81.2 and 56.1%, respectively. Of the adsorbents evaluated, zeolite noticeably increased LEE to 98.7%. Increasing the amount of chloroform by 50% in the presence of zeolite caused a further significant increase in LEE and BC to 98.3 and 75.3%, respectively. These results indicated that biodiesel production from wet microalgae could be enhanced markedly by the addition of adsorbents with increased amounts of organic extraction solvents. © 2015, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.
    Biotechnology and Bioprocess Engineering 06/2015; 20(3):593-598. DOI:10.1007/s12257-014-0600-9