Journal of Tissue Engineering and Regenerative Medicine Impact Factor & Information

Publisher: Wiley InterScience (Service en ligne); Wiley InterScience (Online service), Wiley

Journal description

Current impact factor: 4.43

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 4.428
2012 Impact Factor 2.826
2011 Impact Factor 3.278
2010 Impact Factor 3.534
2009 Impact Factor 3.857
2008 Impact Factor 1.615

Impact factor over time

Impact factor

Additional details

5-year impact 3.70
Cited half-life 3.50
Immediacy index 0.90
Eigenfactor 0.01
Article influence 0.94
Other titles Journal of tissue engineering and regenerative medicine (En ligne), Journal of tissue engineering and regenerative medicine
ISSN 1932-6254
OCLC 300182675
Material type Periodical, Internet resource
Document type Internet Resource, Journal / Magazine / Newspaper

Publisher details


  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Restrictions
    • 12 months embargo
  • Conditions
    • Some journals have separate policies, please check with each journal directly
    • On author's personal website, institutional repositories, arXiv, AgEcon, PhilPapers, PubMed Central, RePEc or Social Science Research Network
    • Author's pre-print may not be updated with Publisher's Version/PDF
    • Author's pre-print must acknowledge acceptance for publication
    • On a non-profit server
    • Publisher's version/PDF cannot be used
    • Publisher source must be acknowledged with citation
    • Must link to publisher version with set statement (see policy)
    • If OnlineOpen is available, BBSRC, EPSRC, MRC, NERC and STFC authors, may self-archive after 12 months
    • If OnlineOpen is available, AHRC and ESRC authors, may self-archive after 24 months
    • Publisher last contacted on 07/08/2014
    • This policy is an exception to the default policies of 'Wiley'
  • Classification
    ​ yellow

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Biofabrication of tissue engineering constructs with tailored architecture and organized cell placement using rapid prototyping technologies is a major research focus in the field of regenerative therapies. This study describes a novel alginate-based material suitable for both cell embedding and fabrication of three-dimensional (3D) structures with predefined geometry by 3D plotting. The favourable printing properties of the material were achieved by using a simple strategy: addition of methylcellulose (MC) to a 3% alginate solution resulted in a strongly enhanced viscosity, which enabled accurate and easy deposition without high technical efforts. After scaffold plotting, the alginate chains were crosslinked with Ca(2+) ; MC did not contribute to the gelation and was released from the scaffolds during the following cultivation. The resulting constructs are characterized by high elasticity and stability, as well as an enhanced microporosity caused by the transient presence of MC. The suitability of the alginate/MC blend for cell embedding was evaluated by direct incorporation of mesenchymal stem cells during scaffold fabrication. The embedded cells showed high viability after 3 weeks of cultivation, which was similar to those of cells within pure alginate scaffolds which served as control. Maintenance of the differentiation potential of embedded cells, as an important requirement for the generation of functional tissue engineering constructs, was proven for adipogenic differentiation as a model for soft tissue formation. In conclusion, the temporary integration of MC in to a low-concentrated alginate solution allowed the generation of scaffolds with dimensions in the range of centimetres without loss of the positive properties of low-concentrated alginate hydrogels with regard to cell embedding. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.
    Journal of Tissue Engineering and Regenerative Medicine 07/2015; DOI:10.1002/term.2058
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study examines the hypothesis that injectable collagen gel can be an effective carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2)'s localization to the healing tendon-bone interface. In 36 mature New Zealand White rabbits, the upper long digital extensor tendon was cut and inserted into the proximal tibial bone tunnel. Then a rhBMP-2-containing collagen gel was injected into the tendon-bone tunnel interface, using a syringe. Histological and biomechanical assessments of the tendon-bone interface were conducted at 3 and 6 weeks after implantation. In vitro testing showed that the semi-viscous collagen gel at room temperature was transformed into a firm gel state at 37°C. The rhBMP-2 release profile showed that rhBMP-2 was released from the collagen gel for more than 28 days. In vivo testing showed that fibrocartilage and new bone are formed at the interface at 6 weeks after injection of rhBMP-2. On radiography, spotty calcification appeared and enthesis-like tissue was produced successfully in the tendon at 6 weeks after injection of rhBMP-2. Use of the viscous collagen gel and rhBMP-2 mixture increased the fusion rate between the bone tunnel and tissue graft. This study demonstrates that viscous collagen gel can be an effective carrier for rhBMP-2 delivery into surgical sites, and that the injectable rhBMP-2-containing collagen gel may be applied for the enhancement of tendon-bone interface healing in the future.
    Journal of Tissue Engineering and Regenerative Medicine 07/2015; DOI:10.1002/term.2041
  • Journal of Tissue Engineering and Regenerative Medicine 06/2014; 8(S1):58.
  • [Show abstract] [Hide abstract]
    ABSTRACT: In drug delivery there has been great interest in the development of nano- and microcarriers of active agents to control their release kinetics and their efficacy by delivering them to where they are most needed. A possible method of delivery is that of carrier internalization by cells. By delivering an active agent directly to the cells, high drug concentrations can be made available to the target cells while circumventing undesirable side effects to surrounding tissues due to premature drug leakage. In this work, microcapsules prepared using layer-by-layer were conceived using chitosan and biomimetic elastin-like recombinamers (ELRs) as constituents assembled onto templates of calcium carbonate microparticles. Two types of ELRs were used: one containing the bioactive aminoacid sequence RGD and the other a scrambled nonfunctional RDG. Scanning electron microscopy (SEM) showed no morphological differences among both types of microcapsules, being spherical with around 4 μm in diameter. Cell viability studies were performed using human mesenchymal stem cells (hMSCs) and microcapsule/cell ratios from 5:1 to 100:1. After 3 and 72 hours of incubation, no significant cytotoxicity was observed in respect to a positive control of hMSCs co-incubated with microcapsules. The cells were kept in culture for another 7 days in absence of microcapsules. Live/dead assays confirmed that cells retained their cellular integrity, thus the contact of hMSCs with either functionalized microcapsule type does not result in cellular death. The internalization efficacy of microcapsules was assessed by flow cytometry and microscopy analysis. To our knowledge, this is the first time that the internalization effectiveness of RGD-functionalized LbL microcapsules is compared with a nonfunctional analogue microcapsule type. Loading them with DQ-ovalbumin permitted to follow the intracellular traffic and degradation by monitoring fluorescence changes. The data indicated that 63% of the hMSCs have internalized RGD-functionalized microcapsules, while their nonfunctional analogue triggered internalization in around 53% of the cells. No statistical differences were found between both cases, suggesting that macropinocytosis should be the major endocytosis mechanism involved in the cellular uptake of this class of carrier devices and that the exhibition of the RGD/RDG motifs does not influence significantly the incorporation of the microcapsules by the hMSCs. Intracellular processing was assessed by qualitative fluorescence variations showing that this phenomenon was faster for the RGD-functionalized microcapsules. The developed multilayer microcapsules using biomimetic ingredients for intracellular delivery let foresee new strategies to increase the availability of molecules of interest in cells and for targeted biomedical applications.
    Journal of Tissue Engineering and Regenerative Medicine 09/2013; 7(S1):16.
  • Journal of Tissue Engineering and Regenerative Medicine 08/2013; Vol. 6(1):(2012) 54-56.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cord blood‐derived haematopoietic stem cells (CB‐HSCs) are an attractive source for transplantation in haematopoietic disorders. However, the yield of CB‐HSCs per graft is limited and often insufficient, particularly for the treatment of adult patients. Here we compare the capacity of three cytokine cocktails to expand CB‐CD34+ cells. Cells were cultured for 5 or 14 days in media supplemented with: (a) SCF, FL, IL‐3 and IL‐6 (SFLIL3/6); (b) SCF, TPO, FGF‐1 and IL‐6 (STFIL6); and (c) SCF, TPO, FGF‐1, IGFBP2 and Angptl‐5 (STFAI). We observed that STFAI‐culture expansion sustained the most vigorous cell proliferation, maintenance of CD34+ phenotype and colony‐forming unit counts. In addition, STFAI‐cultured cells had a potent ex vivo migration activity. STFAI‐expanded cells were able to engraft NSG mice. However, no significant difference in overall engraftment was observed among the expansion cocktails. Assessment of short‐term reconstitution using multilineage markers demonstrated that the STFAI cocktail for HSCs expansion greatly improved total cell expansion but may impair short‐term lymphoid repopulation. Copyright © 2012 John Wiley & Sons, Ltd.
    Journal of Tissue Engineering and Regenerative Medicine 01/2013; 7(12).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Encased in lacunae, osteocytes receive nutrition and biomechanical signals through the lacunocanalicular system. We have developed a novel flow‐perfusion bioreactor designed to support lacunocanalicular fluid flow. We hypothesize that ex vivo fluid flow can maintain endochondral bone viability and, ultimately, serve as a novel model to study bone biology in vitro. Sprague–Dawley rat femurs were harvested, stripped of soft tissue, loaded into a custom‐designed bioreactor and perfused with osteogenic culture medium. After 14 days of flow‐perfusion or static culture, the bones were harvested, fixed, decalcified, embedded, sectioned and stained with haematoxylin and eosin. Fresh long bone samples were similarly processed for comparison. Osteocyte viability and function were also evaluated, using thiazolyl blue tetrazolium bromide (MTT), fluorospectrophotometric DNA quantification, alkaline phosphatase (ALP) colorimetric assay and fluorochrome labelling of mineralizing surfaces. All samples remained free of infection throughout the study period. After 14 days of flow perfusion, histological analysis showed normal‐appearing bony architecture, with 72% of lacunae being osteocyte‐filled compared with 93% in freshly harvested samples and only 36% in static samples. MTT staining and assay confirmed osteocyte viability in the flow‐perfusion samples as well as in fresh samples. DNA quantification demonstrated DNA to be preserved in flow‐perfused samples when compared with freshly harvested samples. ALP activity in flow‐perfusion explants was upregulated compared with fresh and static samples. Fluorochrome‐labelled mineralizing surfaces were seen throughout the explanted flow‐perfused samples. This is the first demonstration that flow perfusion provides adequate chemotransportation to explanted murine endochondal bones. Copyright © 2011 John Wiley & Sons, Ltd.
    Journal of Tissue Engineering and Regenerative Medicine 11/2012; 6(10).
  • Journal of Tissue Engineering and Regenerative Medicine 09/2012; 6(Supplement s1):151.
  • Journal of Tissue Engineering and Regenerative Medicine 09/2012; 6(s1):200.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Genetic studies such as microarray chips generate a large volume of data which must be properly analysed to obtain the biological significance of the resulting list of genes. Data mining techniques are useful to identify and characterize the role of these genes by construction of maps reflecting expression or function. However, the choice of a suitable tool for a given experimental dataset is not straightforward. To establish the most adequate analysis tool based on the characteristics of data, gene lists were studied using different tools. Microarray studies were performed with human adipose stem cells (hASC¢s). We analysed different databases of Agilent gene expression microarrays from hASC¢s employing different bioinformatics software. Background correction was applied by the subtraction method described in LIMMA. Array data were normalized through a non-parametric quantile normalization assuming similar distribution of the signal for all arrays. For differential expression analysis, we treated our data using the LIMMA of bioconductor parametric method, to obtain stable values of differential expression, even with few replicas. To classify the biological functions associated with our differential expression gene lists, we compared some tools for functional analysis of biological enrichment including DAVID and FatiGO. Our observations suggest that software can yield distinct results depending on sample size and functional genes of interest.
    Journal of Tissue Engineering and Regenerative Medicine 09/2012; 6.
  • Journal of Tissue Engineering and Regenerative Medicine 09/2012; 6(S1):174.
  • Journal of Tissue Engineering and Regenerative Medicine 09/2012; 6(1, SI):387.