Journal of Chromatography A (J Chrom)

Publisher: Elsevier

Journal description

The Journal of Chromatography A publishes papers on all aspects of separation science including chromatography, electrochromatography, electrophoresis, hyphenated and other multi-dimensional techniques, sample preparation as well as detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with chromatographic and electrophoretic theory, instrumental developments and their analytical and preparative applications. Section A covers all areas except biomedical sciences and biomedical applications of separation science, which are published in section B: Biomedical Sciences and Applications. Electronic version.

Current impact factor: 4.17

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 4.169
2013 Impact Factor 4.258
2012 Impact Factor 4.612
2011 Impact Factor 4.531
2010 Impact Factor 4.194
2009 Impact Factor 4.101
2008 Impact Factor 3.756
2007 Impact Factor 3.641
2006 Impact Factor 3.554
2005 Impact Factor 3.096
2004 Impact Factor 3.359
2003 Impact Factor 2.922
2002 Impact Factor 3.098
2001 Impact Factor 2.793
2000 Impact Factor 2.551
1999 Impact Factor 2.52
1998 Impact Factor 2.321
1997 Impact Factor 2.697
1996 Impact Factor 2.457
1995 Impact Factor 2.296
1994 Impact Factor 2.523

Impact factor over time

Impact factor

Additional details

5-year impact 4.30
Cited half-life 7.90
Immediacy index 0.62
Eigenfactor 0.07
Article influence 0.85
Website Journal of Chromatography A website
ISSN 1873-3778

Publisher details


  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Authors pre-print on any website, including arXiv and RePEC
    • Author's post-print on author's personal website immediately
    • Author's post-print on open access repository after an embargo period of between 12 months and 48 months
    • Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months
    • Author's post-print may be used to update arXiv and RepEC
    • Publisher's version/PDF cannot be used
    • Must link to publisher version with DOI
    • Author's post-print must be released with a Creative Commons Attribution Non-Commercial No Derivatives License
    • Publisher last reviewed on 03/06/2015
  • Classification

Publications in this journal

  • Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.084
  • [Show abstract] [Hide abstract]
    ABSTRACT: The surface of nanoporous gold (np-Au) monoliths was modified via a flow method with the lectin Concanavalin A (Con A) to develop a substrate for separation and extraction of glycoproteins. Self-assembled monolayers (SAMs) of α-lipoic acid (LA) on the np-Au monoliths were prepared followed by activation of the terminal carboxyl groups to create amine reactive esters that were utilized in the immobilization of Con A. Thermogravimetric analysis (TGA) was used to determine the surface coverages of LA and Con A on np-Au monoliths which were found to be 1.31×10(18) and 1.85×10(15)moleculesm(-2), respectively. An in situ solution depletion method was developed that enabled surface coverage characterization without damaging the substrate and suggesting the possibility of regeneration. Using this method, the surface coverages of LA and Con A were found to be 0.989×10(18) and 1.32×10(15)moleculesm(-2), respectively. The selectivity of the Con A-modified np-Au monolith for the high mannose-containing glycoprotein ovalbumin (OVA) versus negative control non-glycosylated bovine serum albumin (BSA) was demonstrated by the difference in the ratio of the captured molecules to the immobilized Con A molecules, with OVA:Con A=2.3 and BSA:Con A=0.33. Extraction of OVA from a 1:3 mole ratio mixture with BSA was demonstrated by the greater amount of depletion of OVA concentration during the circulation with the developed substrate. A significant amount of captured OVA was eluted using α-methyl mannopyranoside as a competitive ligand. This work is motivated by the need to develop new materials for chromatographic separation and extraction substrates for use in preparative and analytical procedures in glycomics.
    Journal of Chromatography A 11/2015; 1423. DOI:10.1016/j.chroma.2015.10.060
  • [Show abstract] [Hide abstract]
    ABSTRACT: Polymer items are extensively present in the human environment. Humans may be consequently exposed to some compounds, such as additives, incorporated in these items. The objective of this work is to assess the human exposure to the main additives such as those authorized in the packaging for pharmaceutical products. The urinary matrix was selected to optimally answer this challenge because it has already been proven that the exposure to chemicals can be revealed by the analysis of this biological matrix. A multi-residue analytical method for the trace analysis at ng/mL in human urine was developed, and consisted of an extraction of analytes from urine by solid phase extraction (SPE) and an analysis by ultra-high performance liquid chromatography coupled to a tandem mass spectrometer (UHPLC-MS/MS). Even if the quantification of these compounds was an analytical challenge because of (i) the presence of these substances in the analytical process, (ii) the diversity of their physicochemical properties, and (iii) the complexity of the matrix, the optimized method exhibited quantification limits lower than 25ng/mL and recoveries between 51% and 120% for all compounds. The method was validated and applied to 52 human urines. To the best of our knowledge, this work presents the first study allowing the assessment of the occurrence of more than twenty polymer additives at ng/mL in human urine.
    Journal of Chromatography A 11/2015; 1423. DOI:10.1016/j.chroma.2015.10.091
  • [Show abstract] [Hide abstract]
    ABSTRACT: Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.
    Journal of Chromatography A 11/2015; 1423. DOI:10.1016/j.chroma.2015.10.064
  • [Show abstract] [Hide abstract]
    ABSTRACT: This article describes work leading to a microfabricated preconcentrator-focuser (μPCF) designed for integration into a wearable microfabricated gas chromatograph (μGC) for monitoring workplace exposures to volatile organic compounds (VOCs) ranging in vapor pressure from ∼0.03 to 13kPa at concentrations near their respective Threshold Limit Values. Testing was performed on both single- and dual-cavity, etched-Si μPCF devices with Pyrex caps and integrated resistive heaters, packed with the graphitized carbons Carbopack X (C-X) and/or Carbopack B (C-B). Performance was assessed by measuring the 10% breakthrough volumes and injection bandwidths of a series of VOCs, individually and in mixtures, as a function of the VOC air concentrations, mixture complexity, sampling and desorption flow rates, adsorbent masses, temperature, and the injection split ratio. A dual-cavity device containing 1.4mg of C-X and 2.0mg of C-B was capable of selectively and quantitatively capturing a mixture of 14 VOCs at low-ppm concentrations in a few minutes from sample volumes sufficiently large to permit detection at relevant concentrations for workplace applications with the μGC detector that we ultimately plan to use. Thermal desorption at 225°C for 40s yielded ≥99% desorption of all analytes, and injected bandwidths as narrow as 0.6s facilitated efficient separation on a downstream 6-m GC column in <3min. A preconcentration factor of 620 was achieved for benzene from a sample of just 31mL. Increasing the mass of C-X to 2.3mg would be required for exhaustive capture of the more volatile target VOCs at high-ppm concentrations.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.045
  • [Show abstract] [Hide abstract]
    ABSTRACT: Electro membrane extraction-solid-liquid phase microextraction (EME-SLPME) was developed for the first time to determine phenolic contaminants in water. The extraction system consisted of a solid/liquid interface that permitted a three-phase microextraction approach involving an aqueous sample (donor phase): an organic solvent-sorbent within a membrane bag, and an organic solvent (extractant phase), operated in a direct immersion sampling system. The sorbent, reduced graphene oxide/polyvinyl alcohol, synthesized using graphene oxide and polyvinyl alcohol by dispersing the graphene oxide in polyvinyl alcohol and chemically reducing it in aqueous solution. The prepared sorbent was dispersed in 1-octanol and the solution was immobilized by sonication in the membrane bag wall pores which was in contact with the aqueous donor solution and organic extractant solvent (1-octanol) in the main bag itself. The analytes were transported by application of an electrical potential difference of 100V across the sorbent/solvent phase from the aqueous sample into the organic extractant phase in the membrane bag. After extraction and derivatization, gas chromatography-mass spectrometry was used to determine the derivatized analytes. This proposed EME-LSPME procedure provided high extraction efficiency with relative recoveries up to 99.6%. A linearity range of between 0.05 and 100μgL(-1) with corresponding coefficients of determination (r(2)) of between 0.987 and 0.996 were obtained. The limits of detection were in the range of between 0.003 and 0.053μgL(-1). This proposed method was successfully applied to the extraction of phenolic contaminants from water sample.
    Journal of Chromatography A 11/2015; 1423. DOI:10.1016/j.chroma.2015.10.048
  • [Show abstract] [Hide abstract]
    ABSTRACT: This manuscript reports a validated analytical approach for the quantification of 21 water soluble vitamins and their main circulating forms in human plasma. Isotope dilution-based sample preparation consisted of protein precipitation using acidic methanol enriched with stable isotope labelled internal standards. Separation was achieved by reversed-phase liquid chromatography and detection performed by tandem mass spectrometry in positive electrospray ionization mode. Instrumental lower limits of detection and quantification reached <0.1-10nM and 0.2-25nM, respectively. Commercially available pooled human plasma was used to build matrix-matched calibration curves ranging 2-500, 5-1250, 20-5000 or 150-37500nM depending on the analyte. The overall performance of the method was considered adequate, with 2.8-20.9% and 5.2-20.0% intra and inter-day precision, respectively and averaged accuracy reaching 91-108%. Recovery experiments were also performed and reached in average 82%. This analytical approach was then applied for the quantification of circulating water soluble vitamins in human plasma single donor samples. The present report provides a sensitive and reliable approach for the quantification of water soluble vitamins and main circulating forms in human plasma. In the future, the application of this analytical approach will give more confidence to provide a comprehensive assessment of water soluble vitamins nutritional status and bioavailability studies in humans.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.09.049
  • [Show abstract] [Hide abstract]
    ABSTRACT: Extraction of the essential oil from a medicinal plant called Dracocephalum kotschyi Boiss was performed by green technology of supercritical carbon dioxide (SC-CO2) extraction. A Taguchi orthogonal array design with an OA16 (4(5)) matrix was used to evaluate the effects of five extraction variables: pressure of 150-310bar, temperature of 40-60°C, average particle size of 250-1000μm, CO2 flow rate of 2-10ml/s and dynamic extraction time of 30-100min. The optimal conditions to obtain the maximum extraction yield were at 240bar, 60°C, 500μm, 10ml/s and 100min. The extraction yield under the above conditions was 2.72% (w/w) which is more than two times the maximum extraction yield that has been reported for this plant in the literature using traditional extraction techniques. Results from analysis of variance (ANOVA) indicated that the CO2 flow rate and the extraction time were the most significant factors on the extraction yield by percentage contribution of 44.27 and 28.86, respectively. Finally, the chemical composition of the essential oil was evaluated by using gas chromatography-mass spectroscopy (GC-MS). Citral, p-mentha-1,3,8-triene, D-3-carene and methyl geranate were the major components identified.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.040
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, a stable cadmium(II)-based metal-organic framework (MOF) material was designed and used as a sorbent for the dispersive solid-phase extraction (dSPE) of polybrominated diphenyl ethers (PBDEs) in environmental water samples. Gas chromatography coupled with triple quadrupole mass spectrometer (GC-MS/MS), working in the negative chemical ionization mode, was used to quantify the target analytes. Characterization of the material was performed by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), elementary analyses (EA) and thermogravimetric analyses (TGA). The synthesized rod shape MOF is on the micro level in size and has excellent chemical and solvent stability. The extraction conditions, including the extraction time, temperature and ionic strength, were examined systematically using response surface methodology (RSM). Under optimized conditions, the method that was developed showed an excellent extraction performance. Good linearity (R(2)>0.99) within the concentration range of 0.25-250ngL(-1) was obtained. Low limits of detection (0.08-0.15ngL(-1), signal-to-noise ratio=3:1) and good precision (relative standard deviation <12%, n=6) were achieved. The developed method was applied to analyze natural and spiked environmental water samples.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.039
  • [Show abstract] [Hide abstract]
    ABSTRACT: An analytical method for the simultaneous extraction and determination of four different groups of pharmaceuticals in compost from the biodegradation of biological infectious hazardous wastes (BIHW) was developed and successfully validated. Compost samples were spiked with known concentrations of the pharmaceuticals of interest. Ultrasonic extraction with an ethyl acetate and methanol solution (1:1) resulted to be effective for the extraction of eight target compounds. All the compounds were separated in a single gradient run by UHPLC using a Zorbax SB C18 Agilent (2.1×50mm, 1.8μm) column. Analytes were detected and quantified via multiple reaction monitoring (MRM) using an AB SCIEX API-5000TM triple quadrupole with electrospray ionization (ESI) in positive mode. The optimum mobile phase consisted of ammonium formate (2mM, pH 3): MeOH (50:50). Recovery values of the ultrasonic extraction for all compounds were on the order of 87% to 113% with absolute deviations lower than 11%. The limits of detection and quantification for the eight pharmaceuticals were on the order of 0.66ngg(-1) and 2ngg(-1) respectively for all the pharmaceuticals analyzed. These values are lower than those values reported in the literature. Suitable level of linearity, acceptable limits of detection and quantification, good repeatability and inter-day precision, non-ion interference, and low matrix effect resulted from the validation of the analytical method. Implementation of the analytical procedure proposed in this research will contribute in having shorter analysis time and lower costs when working with complex matrices such as compost.
    Journal of Chromatography A 11/2015; 1423. DOI:10.1016/j.chroma.2015.10.051
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study reports a fast and automated analytical procedure for the analysis of aflatoxin M1 (AFM1) in milk and dairy products. The method is based on the simultaneous protein precipitation and AFM1 extraction, by salt-induced liquid-liquid extraction (SI-LLE), followed by an online solid-phase extraction (online SPE) coupled to ultra-high-pressure-liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis to the automatic pre-concentration, clean up and sensitive and selective determination of AFM1. The main parameters affecting the extraction efficiency and accuracy of the analytical method were studied in detail. In the optimal conditions, acetonitrile and NaCl were used as extraction/denaturant solvent and salting-out agent in SI-LLE, respectively. After centrifugation, the organic phase (acetonitrile) was diluted with water (1:9 v/v) and purified (1mL) by online C18 cartridge coupled with an UHPLC column. Finally, selected reaction monitoring (SRM) acquisition mode was applied to the detection of AFM1. Validation studies were carried out on different dairy products (whole and skimmed cow milk, yogurt, goat milk, and powder infant formula), providing method quantification limits about 25 times lower than AFM1 maximum levels permitted by EU regulation 1881/2006 in milk and dairy products for direct human consumption. Recoveries (86-102%) and repeatability (RSD<3, n=6) meet the performance criteria required by EU regulation N. 401/2006 for the determination of the levels of mycotoxins in foodstuffs. Moreover, no matrix effects were observed in the different milk and dairy products studied. The proposed method improves the performance of AFM1 analysis in milk samples as AFM1 determination is performed with a degree of accuracy higher than the conventional methods. Other advantages are the reduction of sample preparation procedure, time and cost of the analysis, enabling high sample throughput that meet the current concerns of food safety and the public health protection.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.094
  • [Show abstract] [Hide abstract]
    ABSTRACT: A fast and non-lethal in vivo solid-phase microextraction (SPME) sampling method for rat blood coupled to liquid chromatography and tandem mass spectrometry (LC-MS/MS) was developed for monitoring rapid changes in concentrations of eicosanoids - lipid mediators involved in the development of inflammatory conditions - using diffusion-based calibration. Sampling rates of target eicosanoids were pre-determined under laboratory conditions with a precision of ≤10%, and directly used for quantification of analyte concentrations in blood after lipopolysaccharide-induced inflammation in Sprague-Dawley rats. Results showed significant changes in unbound plasma concentrations of arachidonic acid (AA) and 12-hydroxyeicosatetraenoic acid (12-HETE) in response to the treatment. Next, performance of the proposed method was compared with protein precipitation (PP) of plasma, a conventional sample preparation technique. Finally, percentages of plasma protein binding (PPB) of specific eicosanoids were determined. PPB of target eicosanoids was in agreement with literature values, ranging from 99.3 to 99.9% for 12-HETE and DHA, respectively. We envision that the proposed method is a particularly suitable alternative to lethal sampling and current methods based on sample depletion in animal studies for accurate monitoring of rapid changes in blood concentrations of small molecules.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.067
  • [Show abstract] [Hide abstract]
    ABSTRACT: The level of co-extracted matrix in wheat and oat extracts obtained by the QuEChERS method (EN 15662) is high and the occurrence of free fatty acids generates a major matrix peak in TIC chromatograms (rt. 13-22min). Matrix can compromise the analytical performance in pesticide analysis using GC-MS/MS. In order to reduce the amount and the effects of matrix we tested the effect of using six different amounts of primary secondary amine (PSA) (0, 25, 50, 100, 150 and 200mg/ml extract) with and without the addition of six different amounts of C18 (0, 25, 50, 100, 150 and 200mg/ml extract) in the dispersive solid phase extraction (dSPE) procedure. dSPE clean-up using 25mg/ml extract significantly reduced the major matrix peak observed for wheat extracts. Higher amounts of PSA reduced the analytical response for iprodione and malathion. For oat extract 50-150mg PSA/ml extract was needed to obtain equally low intensity of the matrix peak. For oat the analytical responses of the target pesticides generally increased with increasing amount of PSA. C18 had no significant effect on the intensity of the major matrix peaks and even resulted in lower analytical responses for several of the target pesticides. Based on the present study it is concluded that the optimal dSPE clean-up procedure employs 25mg PSA/ml extract for wheat and 150mg PSA/ml extract for oat.
    Journal of Chromatography A 11/2015; 1423. DOI:10.1016/j.chroma.2015.10.086
  • [Show abstract] [Hide abstract]
    ABSTRACT: There has been a great emphasis on developing higher-throughput protein purification techniques to screen potential human therapeutics faster and more efficiently. Not only is it desirable to have high-throughput purification for initial screens but it is also desirable to efficiently purify selected protein therapeutics in the amounts and purity required for definitive assays. Current automated tandem technologies involve size exclusion as a second step that often fails to generate the required purity, is not robust and can only be operated at a limited scale. We have modified an ÄKTA to enable in-line dilution, assuring that the automated loading of a second column from a first column elution can be modified to a pH and ionic strength which is suitable for binding to the second column. For example, Protein A can be employed as a first step followed by direct loading on to a cation exchange column by conditioning the Protein A elution using the in-line diluter. Using this method as described, up to six samples of 1L each can be purified through two columns without human intervention per day per machine, and the system produces good yields of purified protein over a wide range of loading levels (12-300mg). In addition, the system employs guanidine HCl regeneration, followed by a sodium hydroxide wash between purification runs, minimizing the possibility of carryover contamination. The system is described at the 5mL and the 10mL column sizes; however, it could readily be programed for 100mL columns to enable larger-scale purifications. Using this system to automate two-column purifications minimizes human intervention, increases efficiency and minimizes the risk of human error.
    Journal of Chromatography A 11/2015; DOI:10.1016/j.chroma.2015.10.092