International Journal of Agriculture and Biology


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    ABSTRACT: Begomovirus disease complexes are the major limiting factor on chilies throughout the Indian subcontinent. The severe symptoms in chilies are associated with multiple begomovirus components. Infectivity assays of Tomato leaf curl New Delhi virus (ToLCNDV) and Chili leaf curl betasatellite (ChLCB) were conducted. DNA A and DNA B of ToLCNDV isolated from chilies and tomato were infectious and produced leaf curl symptoms when inoculated on Nicotiana benthamiana by biolistic gun method. Co-inoculation of ToLCNDV with ChLCB resulted in the severity of disease symptoms. These results show that interaction of betasatellite with bipartite begomoviruses may enhance symptoms induced by bipartite viruses. Thus interactions of betasatellites and symptom enhancement are not limited to monopartite begomoviruses and bipartite begomovirus-betasatellite interaction presents yet another example of rapid changes in begomovirus complexes that infect important crops in the region.
    International Journal of Agriculture and Biology 09/2014; 16(6):1225-1228.
  • International Journal of Agriculture and Biology 08/2014;
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    ABSTRACT: Genetic diversity of 113 desi chickpea genotypes was studied through descriptive, principal component and cluster analysis. High variances were observed for days to flowering, maturity, plant height, pods plant-1, biological yield and harvest index. These traits also showed positive significant correlation with yield which was confirmed through principal component analysis. Principal component analysis (PCA) revealed that the first 4 principal components accounted for 71.99% of the total variation. Seed yield, biological yield, pods plant-1, secondary branches and plant height in chickpea showed positive relation with the first component (PC1). Days to flowering, days to maturity, 100-seed weight showed positive correlation with the second component (PC2). The genotypes were grouped into four clusters using cluster analysis. Genotypes with early flowering and maturity were gathered in cluster I while cluster II showed dominant contribution for grain yield plant-1, harvest index and number of pods plant-1. The grouping of genotypes would be of practical value to chickpea breeders in identifying the genotype with desired trait for utilization in breeding program for genetic improvement.
    International Journal of Agriculture and Biology 06/2014; 16(5):956-960.
  • International Journal of Agriculture and Biology 01/2014; 16:365-370.
  • International Journal of Agriculture and Biology 01/2014; 16:73-80.
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    ABSTRACT: Knowledge about different phosphorus pools in the soil and their relationship with different soil properties is of immense significance for increasing phosphorus use-efficiency in agricultural systems. Fifteen soil series from the relevant agricultural fields were analysed for their phosphorus pools. The average size of different P pools (% of total P) in the soils was in order of, dHCl-P (63.7%) > cHCl-Pi (14.8%) > residual P (9.4%) > cHCl-Po (3.8%) > NaHCO3-Pi (2.4%) > NaOH-Pi (2.0%) > NaHCO3-Po (1.4%) > resin-P (1.3%) > NaOH-Po (1.2%). Overall, the inorganic P pools comprised of 93.6% of total P in the soils and had highly significant positive correlation with soil pH and CaCO3 content. The organic P constituted a small proportion (6.4%) of the total P and was positively correlated with the clay and organic C contents of the soils. The data indicated that organic P pool represents an important source of P in subtropical alkaline soil ecosystems. © 2014 Friends Science Publishers Keywords: Calcareous soils; Inorganic P; Organic P; P pools
    International Journal of Agriculture and Biology 01/2014;
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    ABSTRACT: The risk of brucellosis in the individuals occupationally exposed to animals was determined in present study by slide agglutination test (SAT) and PCR to find best possible option for diagnosis. Peripheral blood samples (n=95) were collected from the humans in and around Faisalabad district, Punjab, Pakistan showing clinical signs related to brucellosis of occupationally exposed groups including veterinary professionals (n=33), livestock farmers (n=48) and butchers (n=14). Brucella genus specific primers were designed (targeting BCSP 31 gene) and used in PCR assay. This could amplify 224-bp region. Out of these 95 peripheral blood samples, 37(38.94%) and 14(14.7%) were positive by SAT and PCR, respectively. PCR is more specific test for detection of Brucella spp. The higher number of positives by SAT as compared to PCR may be due to lack of specificity of SAT. Brucellosis is an occupational hazard in animal handlers and medical practitioners may also focus on this problem while tackling recurrent fever in such population. There is a dire need of more reliable and specific diagnostic facilities like PCR to combat this potentially occupational zoonosis in Pakistan, especially, where brucellosis is prevalent in animals.
    International Journal of Agriculture and Biology 01/2014; 16:986-990.

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