Journal of Innovative Optical Health Sciences

Publications in this journal

• Article: OPTICAL MEASUREMENTS OF RAT MUSCLE SAMPLES UNDER TREATMENT WITH ETHYLENE GLYCOL AND GLUCOSE

  Luís Oliveira, Maria Inês Carvalho, Elisabete Nogueira, Valery Tuchin
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  ABSTRACT: With the objective to study the variation of optical properties of rat muscle during optical clearing, we have performed a set of optical measurements from that kind of tissue. The measurements performed were total transmittance, collimated transmittance, specular re°ectance and total re°ectance. This set of measurements is su±cient to determine di®use re°ectance and absorbance of the sample, also necessary to estimate the optical properties. All the performed measurements and calculated quantities will be used later in inverse Monte Carlo (IMC) simulations to determine the evolution of the optical properties of muscle during treatments with ethylene glycol and glucose. The results obtained with the measurements already provide some information about the optical clearing treatments applied to the muscle and translate the mechanisms of turning the tissue more transparent and sequence of regimes of optical clearing.
  Journal of Innovative Optical Health Sciences 03/2013; 6(2).
• Article: Academic Accreditation Process: Experience of a Medical College in Saudi Arabia

  Mohaimeed
  Journal of Innovative Optical Health Sciences 01/2012;
• Article: Chemical modulation of photodynamic injury of glial cells

  Komandirov M.A, Knyazeva E.A, Fedorenko Y.P, Rudkovskii M.V, Stetsurin D.A, Romanenko N.P, Berezhnaya E.V, Kovaleva V.D, Uzdensky A.B
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  ABSTRACT: Photodynamic therapy based on photogeneration of cytotoxic singlet oxygen and following oxidative stress is currently used in neuro-oncology for destruction of brain tumors. However, along with a tumor, it damages healthy neurons and glial cells. We studied the involvement of the glutamate-related signaling pathway in photodynamic damage to normal glial cells in the crayfish stretch receptor. This model object consists of a single neuron surrounded by glial cells. It was photosensitized with alumophthalocyanine Photosens and irradiated by the diode laser (670 nm). Application of enzyme inhibitors and ion channels modulators showed that exogenous L-glutamate decreased photoinduced apoptosis of crayfish glial cells. The natural neuroglial mediator N-acetylaspartylglutamate, which releases glutamate after splitting by glutamate carboxypeptidase II, also inhibited photoinduced apoptosis. Inhibition of glutamate carboxypeptidase II, oppositely, enhanced glial apoptosis. This confirmed the antiapoptotic activity of glutamate. Glutamate agonist NMDA or inhibitor of NMDA receptors MK801 did not influence photodynamic death of glial cells, i.e., these receptors did not participate in glial apoptosis. Inhibition of metabotropic glutamate receptors mGluRI with AP-3 reduced PDT-induced apoptosis of glial cells. Thus, chemical modifiers of various signaling processes can modulate photoinduced necrosis or apoptosis of glial cells and thus modify efficiency of photodynamic therapy.
  Journal of Innovative Optical Health Sciences 05/2011; 4(4):429-435.
• Article: Non-Invasive Probing of the Neuro-Vascular System in Human Bone/Bone-Marrow using Near-Infrared Light

  T Binzoni, D Van De Ville
  Journal of Innovative Optical Health Sciences 01/2011; 4(2):183-189.
• Article: The evolution of field deployable fNIR spectroscopy from bench to clinical settings

  K. Izzetoglu, H. Ayaz, A. Merzagora, M. Izzetoglu, P. A. Shewokis, S. C. Bunce, K. Pourrezaei, A. Rosen, B. Onaral
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  ABSTRACT: In the late 1980s and early 1990s, Dr. Britton Chance and his colleagues, using picosecond-long laser pulses, spearheaded the development of time-resolved spectroscopy techniques in an e®ort to obtain quantitative information about the optical characteristics of the tissue. These e®orts by Chance and colleagues expedited the translation of near-infrared spectroscopy (NIRS)-based techniques into a neuroimaging modality for various cognitive studies. Beginning in the early 2000s, Dr. Britton Chance guided and steered the collaboration with the Optical Brain Imaging team at Drexel University toward the development and application of a ̄eld deployable con- tinuous wave functional near-infrared spectroscopy (fNIR) system as a means to monitor cog- nitive functions, particularly during attention and working memory tasks as well as for complex tasks such as war games and air tra±c control scenarios performed by healthy volunteers under operational conditions. Further, these collaborative e®orts led to various clinical applications, including traumatic brain injury, depth of anesthesia monitoring, pediatric pain assessment, and brain␣computer interface in neurology. In this paper, we introduce how these collaborative studies have made fNIR an excellent candidate for speci ̄ed clinical and research applications, including repeated cortical neuroimaging, bedside or home monitoring, the elicitation of a posi- tive e®ect, and protocols requiring ecological validity. This paper represents a token of our gratitude to Dr. Britton Chance for his in°uence and leadership. Through this manuscript we show our appreciation by contributing to his commemoration and through our work we will strive to advance the ̄eld of optical brain imaging and promote his legacy.
  Journal of Innovative Optical Health Sciences 01/2011; 4(3):239-250.
• Article: Functional near-infrared spectroscopy-based assessment of attention impairments after traumatic brain injury

  A. C. Merzagora, M. T. Schultheis, B. Onaral, M. Izzetoglu
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  ABSTRACT: A frequent consequence of traumatic brain injury (TBI) is cognitive impairment, which results in signi ̄cant disruption of an individual's everyday living. To date, most clinical rehabilitation interventions still rely on behavioral observation, with little or no quantitative information about physiological changes produced at the brain level. Functional brain imaging has been extensively used in the study of cognitive impairments following TBI. However, its applications to rehabilitation have been limited. This is due in part to the expensive or invasive nature of these modalities. The objective of this study is to apply functional near-infrared spectroscopy (fNIR) to the assessment of attention impairments following TBI. fNIR provides a localized measure of prefrontal hemodynamic activation, which is susceptible to TBI, and it does so in a noninvasive, a®ordable and wearable way, thus partially overcoming the limitations of other modalities. Par- ticipants included 5 TBI subjects and 11 healthy controls. Brain activation measurements were collected during a target categorization task. Signi ̄cant di®erences were found in the hemody- namic response between healthy and TBI subjects. In particular, the elicited responses exhibited reduced amplitude in the TBI group. Overall, the results provide ̄rst evidence of the ability of fNIR to reveal di®erences between TBI and healthy subjects in an attention task. fNIR is therefore a promising neuroimaging technique in the ̄eld of neurorehabilitation. The use of fNIR in neu- rorehabilitation applications would bene ̄t from its noninvasiveness and cost-e®ectiveness and the neurophysiological information obtained through the evaluation of the hemodynamic activation could provide invaluable information to guide the choice of intervention.
  Journal of Innovative Optical Health Sciences 01/2011; 4(3):251-260.
• Article: PHOTOSWITCHABLE NANOFLUOROPHORES FOR INNOVATIVE BIOIMAGING

  Matthew. P. Aldred
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  ABSTRACT: Photosensitive fluorescent probes have become powerful tools in chemical biology and molecular biophysics, which are used to investigate cellular processes with high temporal and spatial resolution. Accordingly, photosensitive fluorescent probes, including photoactivatable, photoconvertible, and photoswitchable fluorophores, have been extensively developed during the past decade. The photoswitchable fluorophores have received much attention because they highlight cellular events clearly. This minireview summarizes recent advances of using reversibly photoswitchable fluorophores and their applications in innovative bioimaging. Photoswitchable fluorophores include photoswitchable fluorescent proteins, photoswitchable fluorescent organic molecules (dyes), and photoswitchable fluorescent nanoparticles. Several strategies have been developed to synthesize photoswitchable fluorophores, including engineering combination proteins, chemical synthesis, polymerization, and self-assembly. Here we concentrate on polymer nanoparticles with optically switchable emission properties: either fluorescence on/off or dual-alternating-color fluorescence photoswitching. The essential mechanisms of fluorescence photoswitching enable different types of photoswitchable fluorophores to change emission intensity or wavelength (color) and thus validating the basis of the fluorescence on/off or dual-color photoswitching design. Generally the possible applications of any fluorophores are to label biological targets, followed by specific imaging. The newly developed photoswitchable fluorophores enable super-resolution fluorescence imaging because of their photosensitive emission. Finally, we summarize the important area regarding future research and development on photoswitchable fluorescent nanoparticles. Read More: http://www.worldscientific.com/doi/abs/10.1142/S1793545811001423
  Journal of Innovative Optical Health Sciences 01/2011; 4(4):395.
• Article: Imaging redox state heterogeneity within individual embryonic stem cell colonies

  He N. Xu, Russell C. Addis, DaVida F. Goings, Shoko Nioka, Britton Chance, John D. Gearhart, Lin Z. Li
  Journal of Innovative Optical Health Sciences 01/2011; 4:279-288.
• Source

  Available from: scnu.edu.cn

  Article: PUMA PROMOTES BAX ACTIVATION IN A FOXO3a-DEPENDENT MANNER IN STS-INDUCED APOPTOSIS

  Yingjie Zhang, Da Xing
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  ABSTRACT: PUMA (p53 up-regulated modulator of apoptosis, also called Bbc3) was first identified as a BH3-only Bcl-2 family protein that is transcriptionally up-regulated by p53 and activated upon p53-dependent apoptotic stimuli, such as treatment with DNA-damaging drugs or UV irra-diation. Recently, studies have shown that PUMA is also up-regulated in response to certain p53-independent apoptotic stimuli, such as growth factor deprivation or treatment with glucocor-ticoids or STS (staurosporine). However, the molecular mechanisms of PUMA up-regulation and how PUMA functions in response to p53-independent apoptotic stimuli remain poorly under-stood. In this study, based on real-time single cell analysis, flow cytometry, and western blotting technique, we investigated the function of PUMA in living human lung adenocarcinoma cells (ASTC-a-1) after STS treatment. Our results show that FOXO3a was activated by STS stimu-lation and then translocated from cytosol to nucleus. The expression of PUMA was up-regulated via a FOXO3a-dependent manner after STS treatment, while p53 had little function in this pro-cess. Moreover, cell apoptosis and Bax activation induced by STS were not blocked by Pifithrin-α (p53 inhibitor), which indicated that p53 was not involved in this signaling pathway. Taken together, these results suggest that PUMA promoted Bax activation in a FOXO3a-dependent pathway during STS-induced apoptosis, while p53 was dispensable in this process.
  Journal of Innovative Optical Health Sciences 02/2010; 1335(3):31-38.
• Article: Quantitative redox scanning of tissue samples using a calibration procedure

  He N. Xu, Baohua Wu, Shoko Nioka, Britton Chance, Lin Z. Li
  Journal of Innovative Optical Health Sciences 01/2009; 2:375-385.
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  Available from: scnu.edu.cn

  Article: SINGLE CELL IMAGING OF BAX TRANSLOCATION DURING APOPTOSIS INDUCED BY PHOTOFRIN-PDT

  Feifan Zhou, Da Xing, Wei R Chen
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  ABSTRACT: Apoptosis is an important cellular event that plays a key role in the therapy of many diseases. The mechanism of the initiation and regulation of photodynamic therapy (PDT)–induced apoptosis is complex. Our previous study found that Photofrin was localized primarily in mitochondria, the primary targets of Photofrin-PDT. The key role of Bax in the mitochondria-mediated apop-tosis has been demonstrated in many systems. In order to determine the role of Bax in the mitochondrion-mediated apoptosis induced by Photofrin-PDT, we used the GFP-Bax plasmid to monitor the dynamics of Bax activation after PDT treatment. With laser scanning confo-cal microscopy, we found that Bax did not translocate from the cytosol to mitochondria when the mitochondrial membrane potential (∆Ψm) disappeared, measured by TMRM. Thus, for Photofrin-PDT, the commitment to cell death is independent of Bax activation.
  Journal of Innovative Optical Health Sciences 01/2009; 926(2):209-214.
• Article: Mitochondrial redox imaging for cancer diagnostic and therapeutic studies

  Lin Z. Li, He N. Xu, Mahsa Ranji, Shoko Nioka, Britton Chance
  Journal of Innovative Optical Health Sciences 01/2009; 2:325-341.
• Article: Quantitative evaluation of retinal tumor volume in mouse model of retinoblastoma by using ultra high-resolution optical coherence tomography.

  MARCO RUGGERI, HASSAN WEHBE, GAVRIIL TSECHPENAKIS, SHULIANG JIAO, MARIA ELENA JOCKOVICH, COLLEEN CEBULLA, ELEUT HERNANDEZ, TIMOTHY G. MURRAY, CARMEN A. PULIAFITO
  Journal of Innovative Optical Health Sciences 06/2008; 01(01):17-28.
• Source

  Available from: psu.edu

  Article: Discrimination of mental workload levels in human subjects with functional near-infrared spectroscopy

  Angelo Sassaroli, Feng Zheng, Leanne M Hirshfield, Audrey Girouard, Erin Treacy Solovey, Robert J K Jacob, Sergio Fantini
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  ABSTRACT: We have applied functional near-infrared spectroscopy (fNIRS) to the human forehead to distinguish different levels of mental workload on the basis of hemodynamic changes occurring in the prefrontal cortex. We report data on 3 subjects from a protocol involv-ing 3 mental workload levels based on to working memory tasks. To quantify the poten-tial of fNIRS for mental workload discrimination, we have applied a 3-nearest neighbor classification algorithm based on the amplitude of oxyhemoglobin (HbO 2) and deoxy-hemoglobin (HbR) concentration changes associated with the working memory tasks. We have found classification success rates in the range of 44%–72%, which are signifi-cantly higher than the corresponding chance level (for random data) of 19.1%. This work shows the potential of fNIRS for mental workload classification, especially when more parameters (rather than just the amplitude of concentration changes used here) and more sophisticated classification algorithms (rather than the simple 3-nearest neighbor algorithm used here) are considered and optimized for this application.
  Journal of Innovative Optical Health Sciences 01/2008; 31(1):227-237.
• Article: A Hybrid Electronic Noses' System Based on Mos-Saw Detection Units Intended for Lung Cancer Diagnosis

  D. I. Wang, K. A. I. Yu, Yishan Wang, Yanjie Hu, Cong Zhao, L. I. N. Wang, Kejing Ying, Ping Wang
  Journal of Innovative Optical Health Sciences 05(01):1150006-1.