Journal of Applied Oral Science (J APPL ORAL SCI)

Publications in this journal

• Article: Associated dental anomalies: case report.

  Daniela Gamba Garib, Nildiceli Leite Melo Zanella, Sheldon Peck
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  ABSTRACT: Certain human dental anomalies frequently occur together, supporting the accumulated evidence of the shared genetic control of dental developmental disturbances. The present study reports a rare and interesting case of a 12-year-old girl with an association of multiple dental abnormalities, including agenesis, tooth malposition and delayed development. The etiology and treatment planning are discussed with reference to the literature. The clinical implications of genetically controlled patterns of dental anomalies are important in the establishment of early diagnosis and appropriate orthodontic intervention.
  Journal of Applied Oral Science 12/2005; 13(4):431-6.
• Article: Masticatory function in temporomandibular dysfunction patients: electromyographic evaluation.

  Giédre Berretin-Felix, Katia Flores Genaro, Inge Elly Kiemle Trindade, Alceu Sergio Trindade Júnior
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  ABSTRACT: Temporomandibular dysfunction (TMD) is a complex disturbance that involves the masticatory muscles and/or temporomandibular joint, causing damage to the masticatory function. This study evaluated the electromyographic activity of the masseter muscle during habitual mastication of bread, apple, banana, cashew nut and paraffin film (Parafilm M) in 25 adult subjects, of both gender, with TMD. The results were compared to those of a control group, composed of 15 adult subjects, of both sexes, free of signs and/or symptoms of TMD. The MYO-TRONICS Inc., K6-I computer software was used for electromyographic processing and analyzed the following parameters: duration of the act, duration of the masticatory cycle and number of cycles. No significant differences were found between subjects in the control group and individuals with TMD as to duration of the masticatory act and of the masticatory cycle, considering all materials used for mastication. The duration of the masticatory act and cycle was longer during mastication of paraffin film in both groups. The number of masticatory cycles was higher for mastication of apple in comparison to mastication of banana, in both groups. It can be concluded that the consistency of foods influences the duration parameters of the act, duration of the cycle and the number of masticatory cycles, and the behavior of the masticatory muscles in individuals with TMD during habitual mastication is similar to that verified in individuals without TMD.
  Journal of Applied Oral Science 12/2005; 13(4):360-5.
• Article: Efficacy of an oral health promotion program for infants in the public sector.

  Marina de Lourdes Calvo Fracasso, Daniela Rios, Maria Gisette Arias Provenzano, Suzana Goya
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  ABSTRACT: This study compared the caries index (dmft), presence of sucking habits and facial alterations in children attended by the preventive program for infants and children assisted by spontaneous demand, at the same age, at the public sector of Maringá, PR. A total of 100 children were evaluated (1-6 years), who were divided into 2 groups: GI - children attended by the infant program with educational and preventive care and bimonthly follow-up, since the 1st year of age; GII - children assisted for conventional treatment (preventive and restorative) according to the needs, and follow-up as requested by the parents. Data collection was performed by interview with the parents and clinical examination for evaluation of the dmft and facial alterations. Analysis of data (Mann-Whitney test for dmft comparison and chi-square test for all other comparisons) demonstrated a significant difference (p<0.05) in relation to the dmft index (GI: 0.2; GII: 3.0), percentage of caries-free children (GI: 88%; GII: 57%) and non-nutritive sucking habits (GI: 47%; GII: 75%). The breastfeeding and bottle utilization practices were similar for both groups. Facial alterations were more frequently observed (p<0.05) in GII (48%) than GI (8.4%), especially for the variables (p<0.05) high arched palate (GI: 10.2%; GII: 35.4%), anterior open bite (GI: 14.2%; GII: 35.4%) and mouth breathing (GI: 6.1%; GII: 29.1%). It was concluded that the infant program is more effective than the spontaneous demand program, reaching the goal of oral health maintenance in the child population.
  Journal of Applied Oral Science 12/2005; 13(4):372-6.
• Article: Compressive strength of resin-modified glass ionomer restorative material: effect of P/L ratio and storage time.

  Mônica Aratani, Antônio Carlos Pereira, Lourenço Correr-Sobrinho, Mário Alexandre Coelho Sinhoreti, Simonides Consani
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  ABSTRACT: The aim of this study was to evaluate the compressive strength of resin-modified glass ionomer cement Fuji II LC and Vitremer, in powder/liquid ratios of 1:1, 1:2 and 1:3, at three periods (24 hours, 7 and 28 days) of storage in distilled water at 37ºC. For each material, P/L ratio and storage time, 5 cylindrical specimens were prepared, with 4mm diameter and 6mm height, in silicon moulds. Specimens were light-cured for 40 seconds at each extremity, removed from the moulds and laterally light-cured (perpendicular to long axis) for 40 seconds, protected as recommended by the manufacturers and immersed for the time tested. The specimens were submitted to compressive strength testing in an Instron machine at a crosshead speed of 1.0mm/min until failure. Data were submitted to ANOVA and Tukey's test (5%), and showed that the compressive strength of resin-modified glass ionomer cement was reduced when P/L ratio was reduced and that the storage in water had little influence on compressive strength.
  Journal of Applied Oral Science 12/2005; 13(4):356-9.
• Article: Correlation between the clinical phonoaudiological assessment and electromyographic activity of the masseter muscle.

  Daiana Cristina Biasotto, Daniela Ap Biasotto-Gonzales, Ivone Panhoca
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  ABSTRACT: The objective of this paper was to evaluate the specificity of masseter muscle palpation when compared to its electromyographic activity. Twenty-one young female adults, aged between 17 and 25, participated in this research. The speech therapy evaluation data were obtained by two speech therapists through masseter muscle palpation during isometric contraction, and the electromyographic exam was achieved by using bipolar superficial electrodes, positioned on the masseter muscle. The volunteers were instructed to chew the Parafilm M® material bilaterally and simultaneously. The capture of the electromiographic signals occurred during masseter muscle isometric contractions. The electrical activity study of the masticatory muscle was analyzed through the Root Mean Square value during its isotonic contraction. All data were then submitted to Kappa statistical analysis. The results of this study have shown a low correlation between palpation and electromyography (25%), as well as between evaluators (40%). According to the findings of the present study, it could be observed that the correlation between masseter muscle palpation and its electromyographic activity was very low, which allows to conclude that muscular palpation cannot effectively replace the eletromyographical exam, but complement it instead.
  Journal of Applied Oral Science 12/2005; 13(4):424-30.
• Article: Antibacterial effect of root canal preparation and calcium hydroxide paste (Calen) intracanal dressing in primary teeth with apical periodontitis.

  Gisele Faria, Paulo Nelson-Filho, Aldevina Campos de Freitas, Sada Assed, Izabel Yoko Ito
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  ABSTRACT: The aim of this study was to evaluate the antibacterial action of root canal mechanical preparation using 2.5% sodium hypochlorite as the irrigating solution and a calcium hydroxide paste as the antibacterial intracanal dressing in human primary teeth root canals with pulp necrosis and apical periodontitis by means of microbial culture. A total of 26 root canals of human primary teeth with pulp necrosis and apical periodontitis were used. Samples were collected before, 72h after biomechanical treatment and 72h after removal of the intracanal dressing. Comparison by Wilcoxon test showed that root canal mechanical preparation effectively eliminated all microorganisms in 20% of the root canals, and the intracanal dressing in 62.5%; however, the cumulative action of biomechanical treatment and intracanal dressing eliminated the microorganisms of 70% of the root canals (p<0.001). Isolated root canal mechanical preparation presented poorer microbiological results that those obtained with root canal mechanical preparation and the use of an intracanal dressing indicating the necessity of topical application of an intracanal medication between sessions in primary teeth with pulp necrosis and apical periodontitis.
  Journal of Applied Oral Science 12/2005; 13(4):351-5.
• Article: Urgency treatment profile of 0 to 15 year-old children assisted at urgency dental service from Bauru Dental School, University of São Paulo.

  Vivien Thiemy Sakai, Ana Carolina Magalhães, Juliano Pelim Pessan, Salete Moura Bonifácio da Silva, Maria Aparecida de Andrade Moreira Machado
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  ABSTRACT: Dental recordings of 0 to 15-year-old patients assisted at Urgency Dental Service (UDS) from Bauru Dental School, University of São Paulo, in 2001 and 2002, were assessed in order to quantify the number of patients that used the service, to determine attendance patterns, and to record the frequency of different types of dental emergencies and their performed treatment. Data were plotted and submitted to a descriptive statistical analysis. Among the total of patients attended at UDS (6020), 1166 (19.37%) were children, with mean age of 9.24 years. Trauma was the cause for 199 (17.06%) of the recorded urgency visits. It occurred more frequently in children between 0 and 3 years of age (34.42%), and between 7 and 12 years of age (18.12%). The main treatments performed were temporary restoration (33.33%) for coronal fracture, and orientation (24.44%) for luxation. Nontraumatic events were the etiology for 967 (82.92%) of the total urgency diagnosis. The most commonly found nontraumatic diagnosis was dental caries lesions (61.75%), followed by problems of eruption or root resorption (14.27%) and bone or soft tissue lesions (6.51%), among others (17.47%). The most frequent treatments performed for caries lesions were: excavation and temporary restoration (39.39%) when there was no abscess, and coronal opening and dressing (40.95%) for caries lesions with abscess. There was an increasing trend in caries lesions prevalence according to the rising of the age, in contrast to trauma prevalence. Treatment for both situations was done according to the indicated protocol for each case.
  Journal of Applied Oral Science 12/2005; 13(4):340-4.
• Article: Fluoride uptake in dental enamel after using fluoridated dentifrice, preceded or not by a CaCl(2) solution rinse.

  Liliane Torsani Ubeda, Vanessa Eid da Silva Cardoso, Marília Afonso Rabelo Buzalaf
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  ABSTRACT: The use of a calcium solution previously to brushing could favor the remineralization and the fluoride (F) uptake in dental enamel. This study evaluated the F in dental enamel after using a F dentifrice, preceded or not by a rinse with a CaCl(2) solution. Ten subjects (18-30 years) participated in a double-blind, cross-over protocol. Enamel biopsy and 3 min saliva samples were obtained at baseline and after brushing (1.5 g Crest®, 1,000 ppm F as NaF), preceded by a rinse with 10 mL of 20 mM CaCl(2) 50 mM acetate pH 7.2 or deionized water, for 1 min, followed by a 15 mL water rinse for 5 sec. Biopsies were made before the rinses and after 8-15 and 120 min. Saliva samples were collected before the rinses and after 4, 15, 30, 60 and 120 min. F concentration in enamel biopsies was analyzed with the F electrode (Orion 9409) and a miniature calomel reference electrode, while phosphorus concentration was analyzed by spectrophotometry. Saliva samples were analyzed for F with the electrode (Orion 9609) by the direct method. The data were analyzed by ANOVA and Tukey test (p<0.05). Mean F concentration ± SD (µg/mL) at baseline up to 120 min for saliva samples were: 0.02±0.01; 9.06±4.26; 2.01±1.76; 0.47±0.33; 0.24±0.17; 0.08±0.08; 0.07±0.04 for the deionized water rinse, and 0.02±0.02; 10.96±14.21; 2.76±3.04; 0.87±1.29; 0.40±0.66; 0.16±0.23; 0.09±0.09 for the CaCl(2) rinse. The data for enamel biopsies were (µg/g): 1861.7±1011.0; 1790.5±953.2; 1117.6±585.2 for the deionized water rinse, and 1586.4±776.8; 1536.3±546.4; 1520.5±1340.7 for the CaCl2 rinse. According to this protocol, there was not a significant variation in enamel F uptake after using a F dentifrice preceded or not by a rinse with a CaCl(2) solution.
  Journal of Applied Oral Science 12/2005; 13(4):418-23.
• Article: Bacteriemia during endodontic treatment in relation to the technique of biomechanical preparation: randomized clinical trial.

  Adriane Tenório Dourado, Arnaldo de França Caldas Junior, Diógenes Ferreira Alves, Creso Abreu Falcão
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  ABSTRACT: The aim of this randomized clinical trial was to evaluate the frequency of bacteriemia during endodontic treatment, with comparison between two techniques for biomechanical preparation of the root canal system. The sample comprised 50 patients aged 16 to 52 years, of both genders, which were divided into 2 groups with 25 patients each. Group I underwent biomechanical preparation by the step-back technique, and Group II was treated by the rotary technique with nickel-titanium instruments (K3). Patients were submitted to antisepsis of the oral cavity with chlorhexidine digluconate and three samples of blood were collected for blood culture: preoperatively, immediately after the biomechanical preparation and 10 minutes later. The significance level adopted was 5.0%, and analysis was performed by descriptive and inferential statistics by means of the Fisher's exact test, Fisher-Freeman-Halton test and Student's t test. Data were analyzed on the Statexact and SPSS softwares. All blood cultures achieved before and immediately after preparation were negative. On the other hand, with regard to the blood cultures collected 10 minutes after preparation, one (4%) positive case was found for Group I. However, this difference was not statistically significant (p = 0.50). The frequency of bacteriemia was low and observed just for Group I.
  Journal of Applied Oral Science 12/2005; 13(4):334-9.
• Article: Partial time use of anterior repositioning splints in the management of TMJ pain and dysfunction: a one-year controlled study.

  Paulo César Rodrigues Conti, João Evandro Silva Miranda, Ana Cláudia C Ferreira Conti, Luiz Fernando Pegoraro, Carlos Dos Reis Pereira de Araújo
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  ABSTRACT: This study aimed at evaluating the effectiveness of partial use of anterior repositioning appliances in the management of TMJ pain and dysfunction when compared to stabilization splints and a control group in a one-year follow-up. Sample was initially constituted by 60 patients, randomly divided into three groups: I- stabilization splints, II- repositioning splints and III- no treatment. The whole sample was evaluated by means of TMJ and muscle palpation, mandibular AROM, analysis of occlusal contacts, joint sounds inspection and Visual Analogue Scale (VAS) for one year; 52 patients composed the final sample. A significant (after 15 days) improvement in pain report (VAS) and palpation index was found for group II (p < or = 0.01). The occurrence of occlusal alterations as posterior open bite or gross interferences after the splint therapy and increased muscle tenderness were not problems in this study. Similar results in joint noises reduction were observed for the entire sample. It was concluded that controlled partial use of repositioning splints is a beneficial tool in the management of intra-articular pain and dysfunction, with no risks of irreversible occlusal changes.
  Journal of Applied Oral Science 12/2005; 13(4):345-50.
• Article: Study on the origin and nature of the adenomatoid odontogenic tumor by immunohistochemistry.

  Marcelo Macedo Crivelini, Ana Maria Pires Soubhia, Renata Callestini Felipini
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  ABSTRACT: The adenomatoid odontogenic tumor (AOT) is a clinically benign lesion. Discussions about the AOT hamartomatous or neoplastic nature, and the probable odontogenic epithelial cell it originates from still exist. This research aimed to study and discuss the subject by the immunohistochemical detection of cytokeratins, laminin, collagen IV, PCNA and p53 in 8 tumor samples and 8 dental follicle samples containing reduced enamel epithelium. The results have shown that CK14 labelling indicated differentiation grades for secreting ameloblasts or ameloblasts in the post-secreting stage in the adenomatoid structure of AOT. Laminin, found on the luminal surface of adenomatoid structures, was compatible with the reduced enamel epithelium during the "protective stage of amelogenesis". PCNA specifically labelled the spindled areas and peripheral cords of the AOT, indicating that these areas are responsible for tumor growth. After considerations about pathogenesis, the authors suggested that the nature of AOT is hamartomatous with histogenesis from the reduced enamel epithelium.
  Journal of Applied Oral Science 12/2005; 13(4):406-12.
• Article: Microscopic analysis of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue.

  Willian Fernando Zambuzzi, Rodrigo Cardoso de Oliveira, Danilo Alanis, Renato Menezes, Ariadne Letra, Tânia Mary Cestari, Rumio Taga, José Mauro Granjeiro
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  ABSTRACT: The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue.
  Journal of Applied Oral Science 12/2005; 13(4):382-6.
• Article: Marginal fit at cylinder-abutment interface before and after overcasting procedure.

  Luciana Martins Cres Moraes, Paulo Henrique Orlato Rossetti, Leylha Maria Nunes Rossetti, Ana Paula Ribeiro do Vale Pedreira, Accacio Lins do Valle, Wellington Cardoso Bonachela
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  ABSTRACT: The aim of this study was to measure marginal fit at cylinder-abutment interface, before and after overcasting procedure. A hexagonal implant was fixed to a stainless steel base and a Estheticone-like abutment used during all the experiment. Before casting procedure, gold (Group I) and Ni-Cr-Be (Group II) premachined cylinders were tightened to the abutment with gold and titanium screws (in both groups), with 10Ncm and 20Ncm torque values for the same screw type. Vertical measures were taken at the light microscope (Mitutoyo 5050, Tokyo, Japan) three times in six different parts along the abutment-cylinder interface for each torque value. Cylinders were overcast with Ag-Pd (Group I) or Ni-Cr-Be (Group II) alloy. After casting, the same measures and torque values were repeated. Intragroup differences (10 or 20Ncm torque values, before and after casting) and intergroup differences (10 and 20Ncm torque values, before or after casting) were analyzed by the Paired t Test; (p<0.05). Intragroup differences were observed for G-I (gold, 20Ncm, titanium screw, p=0.044) and for G-II (Ni-Cr-Be, 10Ncm, gold screw, p=0.002). Intergroup differences only were not observed in the G-II group (Ni-Cr-Be, 10 and 20Ncm, titanium screw, p=0.534). Within the limits of this study, the following conclusions can be drawn: 1) Regardless of screw type, marginal misfit was higher for Ni-Cr-Be cylinders after casting, but within acceptable levels of fit; 2) The combination of gold cylinders with gold or titanium screws was the most effective to reduce marginal misfit; 3) Both screw types did not improve marginal fit of Ni-Cr-Be cylinders after overcastting, and 4) Although a 20Ncm torque improved marginal fit in all situations, its use is not recommended due to the increased risk of prosthesis failure. Ni-Cr-Be alloys may be successfully used in single-tooth implant restorations, with no damage to the fit between abutment and prosthetic cylinder.
  Journal of Applied Oral Science 12/2005; 13(4):366-71.
• Article: Titanium tetrafluoride and dental caries: a systematic review.

  Rubiane Diógenes Alves, Tatyana Maria Silva de Souza, Kenio Costa de Lima
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  ABSTRACT: The aim of this systematic review was to evaluate the effectiveness of titanium tetrafluoride as a preventive or cariostatic agent against caries. The databases used to find the articles analyzed were MEDLINE LILACS, and BBO. In MEDLINE and LILACS the search strategy utilized was "titanium" [Words] and "tetrafluoride" [Words] and Spanish or English or Portuguese [Language], whereas In BBO "titânio" [Words] and "tetrafluoreto" [Words] and Espanhol or Inglês or Português [Language]. Out of a total of 42 studies found, which assessed possible preventive/cariostatic effects of titanium tetrafluoride against caries in vivo, only 2 were selected. In both studies, titanium tetrafluoride was shown to be effective against caries. However, given that the quality and consequently the validity of these two clinical studies are questionable, their results do not allow to conclude that titanium tetrafluoride is effective against caries clinically.
  Journal of Applied Oral Science 12/2005; 13(4):325-8.