Genetics and molecular research: GMR (GENET MOL RES )

Publisher: Fundação de Pesquisas Científicas de Ribeirão Preto


Genetics and Molecular Research (GMR) publishes research articles, research reports, technical notes, scientific commentaries, news, views and review articles on Genetics, Evolution and Molecular Biology. It is an exclusively online journal.

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    Document, Periodical, Internet resource
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Publications in this journal

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    ABSTRACT: Croton membranaceus aqueous root extract (CMARE) is among the widely used phytotherapeutics in Ghana for the management of benign prostatic hyperplasia (BPH) and prostate cancer. However, the mechanism of action of CMARE remains to be elucidated. This study aimed to establish whether apoptosis is involved in the antiproliferative effect of CMARE on human BPH-1 cells. We determined the effect of treatment with 0, 1, 3, and 5 mg/mL CMARE for 24, 48, and 72 h on the viability and morphology of BPH-1 cells using the MMT assay and phase-contrast microscopy, respectively. We examined the apoptosis-inducing effects of CMARE after 48 h at the cellular level using Hoescht 33258 and JC-1 dye staining and flow cytometry analysis. We performed reverse transcription polymerase chain reaction and Western blotting to confirm the apoptotic effects of CMARE at the molecular level. CMARE induced a significant dose-dependent inhibition in the proliferation of BPH-1 cells (P < 0.05) and an alteration in their morphology and a reduction their density. Furthermore, CMARE induced dose-dependent staining of the nuclear chromatin, significant DNA fragmentation with G0/G1 sub-diploid cells (P < 0.01), and loss of the mitochondrial membrane potential in the treated cells compared to the controls after 48 h (P < 0.01). Additionally, while CMARE induced a significant upregulation of the mRNA and protein levels of Bax, those of Bcl2 did not change significantly. Therefore, induction of mitochondria-dependent apoptosis of BPH-1 cells may be a possible mechanism of action of CMARE.
    Genetics and molecular research: GMR 01/2015; 14(1):149-162.
  • Genetics and molecular research: GMR 12/2014; 4(2014).
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    ABSTRACT: Weight gain is a key performance trait for beef cattle; however, attention should be given to the production costs for better profitability. Therefore, a feed efficiency trait based on performance can be an interesting approach to improve performance without increasing food costs. To identify candidate genes and genomic regions associated with residual body weight gain (RWG), we conducted a genome-wide association study (GWAS) with 720 Nellore cattle using the GRAMMAR-Gamma association test. We identified 30 significant single nucleotide polymorphisms (SNPs), especially on chromosomes 2, 8, 12, and 17. Several genes and quantitative train loci (QTLs) present in the identified regions were appointed; we highlight DMRT2 (doublesex and mab-3 related transcription factor 2), IFFO2 (intermediate filament family orphan 2), LNX2 (ligand of numb-protein X 2), MTIF3 (mitochondrial translational initiation factor 3), and TRNAG-CCC (transfer RNA glycine anticodon CCC). The metabolic pathways that can explain part of the phenotypic variation in RWG are related to oxidative stress and muscle control.
    Genetics and molecular research: GMR 12/2014;
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    ABSTRACT: SPOP protein has been identified to have ubiquitin ligase activity. Mutation in SPOP gene has been reported recently in some cancers like prostate, gastric, colorectal cancer. We investigated the SPOP DNA mutation in tumor tissues, collected from 70 Chinese female breast cancer patients in Southwestern of China by DNA sequencing. The results did not show mutation in our collected tissue samples, and here indicated that mutation in SPOP gene might not be associated with breast cancer, particularity in Chinese women. This DNA mutation analysis or DNA genotyping might provided useful and important information for the genetic counseling and personalized medicine treatment from different types of cancers.
    Genetics and molecular research: GMR 11/2014; In Press.