Electromagnetic Biology and Medicine (ELECTROMAGN BIOL MED)

Publications in this journal

• Article: Effects of the ELF-MFs on the development of spleens of preincubated chicken embryos

  Daryush Minaei Tehrani Maryam Shams Lahijani, Fahimeh Varzideh
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  ABSTRACT: Effects of extremely low-frequency magnetic fields (ELF-MFs) on the histopathology, histomorphometry, ultrastructural changes (transmission electron microscope, TEM), apoptosis and alkaline phosphatase (ALP) activities in the spleens of preincubated white leghorn chicken embryo were investigated. Ninety fertilized eggs were divided into six groups as control (n ¼ 15), sham (n ¼ 15) and four experimental groups (1–4, n ¼ 15). Eggs of experimental groups were exposed to 1.33, 2.66, 5.52 and 7.32 mTs flux intensities established in our previous published experiments and the last intensity was used for studying apoptosis and ultrastructures (TEM) of the spleens of 19-day-old chicken embryos. Eggs of control groups remained intact. Sham groups were placed inside the coil for 24 h before incubation with no exposures, then they were incubated in the same incubator (378C, 60% humidity) for 19 days. Spleens of chicken embryos were removed, fixed in formalin (10%), and stained with H&E for histopathological and histomorphometrical surveys; TUNEL assay indicated possible change in apoptosis and TEM and biochemical studies were also carried out. Significant decreases in the sizes of the spleens of embryos of experimental groups, hyperemia, damages in spleen parenchyma, decreases in the numbers of splenic nodules, increases in the number of polymorphonuclear cells and sinusoidal spaces of spleens, significant increase in the level of ALP activities, significant increases in the numbers of apoptotic cells, deformed nuclei and swollen mitochondria were observed in experimental groups comparing with those in control and sham groups. ELF-MF created changes which could impair the immune functions.
  Electromagnetic Biology and Medicine 01/2013;
• Article: The impact of lower induction values of 50 Hz external electromagnetic fields on in vitro T lymphocyte adherence capabilities.

  Coček A, Hahn A, Mártonová J, Ambruš M, Dohnalová A, Nedbalová M, Jandová A
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  ABSTRACT: The authors were monitoring adherence ability of T lymphocytes in vitro in patients with laryngeal and pharyngeal carcinoma at the presence of tumor-specific and viral LDH antigen. The results were assessed and expressed in percent of non adherent T lymphocytes (NAL). First, NAL in patients before initiating the treatment was compared with NAL control group (voluntary blood donors). The ability of the adherence in T lymphocytes in the control group is statistically significantly higher. Further on, NAL in the course of a successful oncological treatment was monitored at the interval of 6 months following the treatment, and further on at yearly intervals. NAL level drops statistically significantly within 6 months and then hold on at levels with no statistical difference unlike the control group, however, the ability of T lymphocyte in patients to adhere remains statistically significantly lower. Statistically significantly higher levels of NAL are at the presence of LDH viral antigen. Further on, the authors were following the influence of magnetic sinusoidal field of power frequency (50 Hz) of a low induction (0.5, 0.1, and 0.05 mT) on NAL. NAL values under the influence of an experimental magnetic field before initiating the treatment as well as in the course of a successful oncological treatment are statistically significantly lower. It means that magnetic filed increases the adherence ability of T lymphocytes in patients with laryngeal and pharyngeal cancer in vitro.
  Electromagnetic Biology and Medicine 06/2012; 31(2):166 - 77.
• Article: The effect of different strengths of electric fields on antioxidant status, lipid peroxidation, and visual evoked potentials

  A1. Akpinar Deniz; Ozturk Nihal; Ozen Sukru; Agar Aysel; Yargicoglu Piraye
  Electromagnetic Biology and Medicine 01/2012; 31(4):2012.
• Article: Analgesic effect of the electromagnetic resonant frequencies derived from the NMR spectrum of morphine

  Ioannis I. Verginadis, Yannis V. Simos, Anastasia P. Velalopoulou, Athina N. Vadalouca, Konstatinos S. Havelas, Vicky P. Kalfakakou, Spyridon Ch. Karkabounas, Angelos M. Evangelou
  Electromagnetic Biology and Medicine 01/2011;
• Article: The Production of Tumor Necrosis Factor in Cells of Tumor-Bearing Mice After Total-Body Microwave Irradiation and Antioxidant Diet

  Elena G. Novoselova, Vyacheslav B. Ogay, Oksana V. Sorokina, Olga V. Glushkova, Oksana A. Sinotova, Evgeny E. Fesenko
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  ABSTRACT: The effects of repeated treatment with weak microwaves (MW) (8.15–18 GHz, 1 µW/cm2, 1.5 h daily) and diet with antioxidants (AO) (β-carotene, α-tocopherol, and ubiquinone Q9) on production of tumor necrosis factor (TNF) in macrophages and T lymphocytes of healthy and tumor-bearing mice (TBM) were studied. Tumor size and mortality of TBM were also followed. Microwave radiation and antioxidant diet stimulated production of TNF in cells from healthy mice. At early stages, tumor growth induced TNF production in mouse cells; however, this effect decreased as tumors grew. In TBM exposed to MW, TNF production was higher than in unirradiated TBM. Oppositely, AO diet induced TNF production in healthy mice but did not affect TNF secretion in TBM. Accordingly, prolonged treatment of TBM to MW, but not to AO diet, decreased tumor growth rate and increased overall animal longevity. These results suggest that diminished tumor growth rate due to extremely low-level MW exposure of mice carrying tumors, at least in part, was caused by enhancement in TNF production and accumulation of plasma TNF.
  Electromagnetic Biology and Medicine 07/2009; 23(2):167-180.
• Article: Changes of leukocyte adherence ability under the influence of magnetic field in the course of a treatment of patients with laryngeal and pharyngeal carcinoma

  Cocek A, Hahn A, Ambrus M, Dohnalová A, Jandová A, Pokorný J
  [show abstract] [hide abstract]
  ABSTRACT: The authors were monitoring adherence ability of T lymphocytes in vitro in patients with laryngeal and pharyngeal carcinoma at the presence of tumor-specific and viral LDH antigen. The results were assessed and expressed in percent of non adherent T lymphocytes (NAL). First, NAL in patients before initiating the treatment was compared with NAL control group (voluntary blood donors). The ability of the adherence in T lymphocytes in the control group is statistically significantly higher. Further on, NAL in the course of a successful oncological treatment was monitored at the interval of 6 months following the treatment, and further on at yearly intervals. NAL level drops statistically significantly within 6 months and then hold on at levels with no statistical difference unlike the control group, however, the ability of T lymphocyte in patients to adhere remains statistically significantly lower. Statistically significantly higher levels of NAL are at the presence of LDH viral antigen. Further on, the authors were following the influence of magnetic sinusoidal field of power frequency (50 Hz) of a low induction (0.5, 0.1, and 0.05 mT) on NAL. NAL values under the influence of an experimental magnetic field before initiating the treatment as well as in the course of a successful oncological treatment are statistically significantly lower. It means that magnetic filed increases the adherence ability of T lymphocytes in patients with laryngeal and pharyngeal cancer in vitro.
  Electromagnetic Biology and Medicine 01/2008; 27(3):277 - 88.
• Article: Ion cyclotron resonance as a tool in regenerative medicine.

  Lisi A, Ledda M, de Carlo F, Pozzi D, Messina E, Gaetani R, Chimenti I, Barile L, Giacomello A, D'Emilia E, Giuliani L, Foletti A, Patti A, Vulcano A, Grimaldi S
  Electromagnetic Biology and Medicine 01/2008; 27(2).
• Article: Case study: amyotrophic lateral sclerosis.

  Cesare Santi, Alberto Turco
  Electromagnetic Biology and Medicine 02/2007; 26(4):329-30.
• Article: Effects of weak, low-frequency pulsed electromagnetic fields (BEMER type) on gene expression of human mesenchymal stem cells and chondrocytes: an in vitro study.

  Markus Walther, Florian Mayer, Wolf Kafka, Norbert Schütze
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  ABSTRACT: In vitro effects of electromagnetic fields appear to be related to the type of electromagnetic field applied. Previously, we showed that human osteoblasts display effects of BEMER type electromagnetic field (BTEMF) on gene regulation. Here, we analyze effects of BTEMF on gene expression in human mesenchymal stem cells and chondrocytes. Primary mesenchymal stem cells from bone marrow and the chondrocyte cell line C28I2 were stimulated 5 times at 12-h intervals for 8 min each with BTEMF. RNA from treated and control cells was analyzed for gene expression using the affymetrix chip HG-U133A. A limited number of regulated gene products from both cell types mainly affect cell metabolism and cell matrix structure. There was no increased expression of cancer-related genes. RT-PCR analysis of selected transcripts partly confirmed array data. Results indicate that BTEMF in human mesenchymal stem cells and chondrocytes provide the first indications to understanding therapeutic effects achieved with BTEMF stimulation.
  Electromagnetic Biology and Medicine 02/2007; 26(3):179-90.
• Article: Ion parametric resonance: resolving the signal-to-noise-ratio paradox.

  Stefan Machlup
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  ABSTRACT: Lednev's (1991) "possible mechanisms for the influence of weak magnetic fields on biological system" involved two parallel magnetic fields, one constant and one oscillatory in the ELF (extremely low-frequency) range. The suggested ion resonances (IPR) were termed "impossible" by Adair (1992, 1997, 1998) even after they were demonstrated in a rat-nerve (PC-12) cell culture by Blackman et al. (1994). The "signal-to-noise-ratio" paradox (introduced by the author) is resolved by taking account of the coherent absorption of the ELF energy and showing how the energy of several trillion ELF photons can free a single ion from its trap on the surface of a cell of the culture.
  Electromagnetic Biology and Medicine 02/2007; 26(3):251-6.
• Article: Effects of prooxidants on human serum exposed to 50 Hz magnetic fields.

  Violeta Calota, Simona Dragoiu, Aurelia Meghea, Maria Giurginca
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  ABSTRACT: The purpose of this article is to evaluate magnetic field effects (50 Hz, different magnetic intensities) on the chemiluminescence intensity of human serum. We find that 1 and 2 h of exposure increased the chemiluminescence emission. The addition to the serum of prooxidants FeCl(2) and H(2)O(2) in different concentrations increased the chemiluminescence intensity even more.
  Electromagnetic Biology and Medicine 02/2007; 26(2):135-40.
• Article: Comparison of bioactivity between GSM 900 MHz and DCS 1800 MHz mobile telephony radiation.

  Dimitris J Panagopoulos, Evangelia D Chavdoula, Andreas Karabarbounis, Lukas H Margaritis
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  ABSTRACT: An increasing number of studies find that pulsed Radio Frequency (RF), electromagnetic radiation of both systems of digital mobile telephony, established and commonly used in Europe during the last years, GSM 900 MHz (Global System for Mobile telecommunications) and DCS 1800 MHz (Digital Cellular System), exert intense biological action on different organisms and cells (Hardell et al., 2006; Hyland, 2000; Kundi, 2004; Panagopoulos et al., 2004, 2007). The two types of cellular telephony radiation use different carrier frequencies and give different frequency spectra, but they usually also differ in intensity, as GSM 900 MHz antennas operate at about double the power output than the corresponding DCS 1800 MHz ones. In our present experiments, we used a model biological system, the reproductive capacity of Drosophila melanogaster, to compare the biological activity between the two systems of cellular mobile telephony radiation. Both types of radiation were found to decrease significantly and non thermally the insect's reproductive capacity, but GSM 900 MHz seems to be even more bioactive than DCS 1800 MHz. The difference seems to be dependent mostly on field intensity and less on carrier frequency.
  Electromagnetic Biology and Medicine 02/2007; 26(1):33-44.
• Article: Local and holistic electromagnetic therapies.

  A R Liboff
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  ABSTRACT: Based on decades of experimental evidence an excellent argument can be made for the existence of a fundamental functional relationship between living systems and electromagnetic fields. We have previously hypothesized that this relationship can be expressed in terms of a field vector whose source is the distribution of electric polarization within the system and which has both a phylogenetic and ontogenetic time dependence. Ion cyclotron resonance (ICR)-like magnetic signals have resulted in physiologic changes in many in vitro and in vivo model systems and have been applied medically with success to bone repair and spinal fusion. This type of local ICR-like therapy has recently been broadened into a holistic application following the remarkable discovery that the whole-body bioimpedance is sharply dependent on ICR signals. We relate this observation to the integrated electric polarization vector, in turn a measure of the double layer charge distribution at the cell membrane. This discovery, already being applied to a number of clinical problems, lends strong support to the concept of an overarching electromagnetic framework for living systems.
  Electromagnetic Biology and Medicine 02/2007; 26(4):315-25.
• Article: Cyclotronic ion resonance therapy and arthralgia.

  Annantonia Ciafaloni
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  ABSTRACT: A total of 143 patients suffering various musculoskeletal disorders including rheumatoid arthritis, arthritis, osteoporosis, and post-surgical discomfort were subjected to ELF magnetic treatments using the Seqex device. A clear trend in pain reduction was observed over the 10 treatment regimen as well as a stabilization of relevant lab tests, including cholesterol level and blood pressure. Improvements were also noted in posturometric footboard tests. An additional 20 patients with various neuromuscular difficulties were treated with Seqex as well as magentic "concentrators" for periods ranging from 3 to 10 treatments. Similar improvements in pain reduction were observed in this smaller group.
  Electromagnetic Biology and Medicine 02/2007; 26(4):299-303.
• Article: Effect of hydrogen peroxide on neuron sensitivity to acetylcholine.

  Arsen Hunanyan, Sinerik Ayrapetyan
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  ABSTRACT: The dose-dependent effect of hydrogen peroxide on snail neuromembrane chemosensitivity was studied by means of standard voltage-clamp method. Short-term exposure (7 min) of neurons to H(2)O(2) (10(-11)-10(-4) M) caused dose-dependent depression of Acetylcholine (Ach)-induced ionic currents in the membrane. The H(2)O(2)-induced depression of Ach-sensitivity of membrane was more pronounced in K(+)-free solution than in normal physiological solution and it disappeared in cold medium (5 degrees C). The H(2)O(2) (10(-11)-10(-4) M) decreased membrane electrical conductivity and cell volume. The dose-dependent decrease in Ach-sensitivity of the snail neuromembrane by H(2)O(2) may be due to a decrease in the number of functionally active membrane receptors caused by a decrease in membrane active surface. H(2)O(2)-induced decrease in Ach-sensitivity has a metabolic but Na(+)-K(+) pump independent character, the nature of which is the subject for current investigation.
  Electromagnetic Biology and Medicine 02/2007; 26(3):225-33.
• Article: Effects of different extremely low-frequency electromagnetic fields on osteoblasts.

  Xiaojun Zhang, Jianbao Zhang, Xuemin Qu, Jun Wen
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  ABSTRACT: It is well known that the extremely low-frequency electromagnetic field (EMF) can promote the healing of bone fractures, but its mechanism remains poorly understood. The purpose of this study was to examine the response of neonatal rat calvarial bone cells to the rectangular electromagnetic field (REMF), triangular electromagnetic field (TEMF), sinusoidal electromagnetic field (SEMF), and pulsed electromagnetic field (PEMF). The stimulatory effects of EMF were evaluated by the proliferation (methyltetrazolium colorimetric assay), differentiation (alkaline phosphatase (ALP) activity), and mineralization (area of mineralized nodules of the cells). REMF treatment of osteoblasts increased cellular proliferation and decreased ALP activity (p < 0.05). TEMF had an accelerative effect on the cellular mineralized nodules (p < 0.05). SEMF treatment of osteoblasts decreased the cellular proliferation, increased ALP activity, and suppressed mineralized nodules formation (p < 0.05). PEMF promoted the proliferation of osteoblasts, inhibited their differentiation, and increased the mineralized nodules formation (p < 0.05). Moreover, the effects of PEMF on osteoblasts were concerned with the extracellular calcium, P2 receptor on the membrane, and PLC pathway, but the response of osteoblasts on SEMF was only related to PLC pathway. The results suggested that the waveforms of EMF were the crucial parameters to induce the response of osteoblasts.
  Electromagnetic Biology and Medicine 02/2007; 26(3):167-77.
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  Article: A possible effect of electromagnetic radiation from mobile phone base stations on the number of breeding house sparrows (Passer domesticus).

  Joris Everaert, Dirk Bauwens
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  ABSTRACT: A possible effect of long-term exposure to low-intensity electromagnetic radiation from mobile phone (GSM) base stations on the number of House Sparrows during the breeding season was studied in six residential districts in Belgium. We sampled 150 point locations within the 6 areas to examine small-scale geographic variation in the number of House Sparrow males and the strength of electromagnetic radiation from base stations. Spatial variation in the number of House Sparrow males was negatively and highly significantly related to the strength of electric fields from both the 900 and 1800 MHz downlink frequency bands and from the sum of these bands (Chi(2)-tests and AIC-criteria, P<0.001). This negative relationship was highly similar within each of the six study areas, despite differences among areas in both the number of birds and radiation levels. Thus, our data show that fewer House Sparrow males were seen at locations with relatively high electric field strength values of GSM base stations and therefore support the notion that long-term exposure to higher levels of radiation negatively affects the abundance or behavior of House Sparrows in the wild.
  Electromagnetic Biology and Medicine 02/2007; 26(1):63-72.
• Article: Effects of endogenous cyclotronic ionic resonance (ICR) on macular diabetic edema: preliminary results.

  P D'Andrea, L Maurizio
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  ABSTRACT: The intent in this research was to verify the effects of the application of low frequency magnetic fields to cases of macular diabetic edema. We treated six patients afflicted by non-proliferating diabetic retinopathy with macular oedema. Quantitative clinical appraisals of the retinal thickness were obtained for the Optical Coherence Tomography (OCT I). None of the cases affected by non-cystoid macular oedema (non-CMO), or with a relevant ischemic component, evidenced by retinal fluorangiography, had further worsening in their clinical course during the treatment. Only one of the patients, who underwent a long treatment period with ICR demonstrated a significant reduction of the macular edema, with no need of other invasive therapeutic procedures (intravitreous injection of triamcinolone and/or laser therapy).
  Electromagnetic Biology and Medicine 02/2007; 26(4):293-8.
• Article: Effects of cyclotronic ion resonance on human metabolic processes: a clinical trial and one case report.

  G Vallesi, F Raggi, S Rufini, S Gizzi, E Ercolani, R Rossi
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  ABSTRACT: We studied the effects of ion cyclotron resonance (Seqex) magnetic therapy on the blood of thirty two healthy volunteers. They received 15 treatments each 27 minutes in length, distributed over 5 weeks. The concentrations of two blood components, malondialdehyde (MDA) and cholesterol were measured in each subject, immediately before and immediately after the 15 treatments as well as one month after the final treatment. Highly significant reductions in MDA concentrations, averaging 53.8% were noted just after the 15 treatments, tending to return to the original concentrations one month later. The effect on HDL and LDL cholesterol levels were not significant. The implication of this work is that this type of therapy may be useful in dealing with oxidative stress.
  Electromagnetic Biology and Medicine 02/2007; 26(4):283-8.