Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Publisher: Elsevier

Journal description

Current impact factor: 2.37

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 2.371
2012 Impact Factor 2.167
2011 Impact Factor 2.235
2010 Impact Factor 2.134
2009 Impact Factor 2.196
2008 Impact Factor 1.709
2007 Impact Factor 1.863
2006 Impact Factor 1.553
2005 Impact Factor 1.351
2004 Impact Factor 1.635
2003 Impact Factor 1.556
2002 Impact Factor 1.274
2001 Impact Factor 1.026
2000 Impact Factor 0.883
1999 Impact Factor 0.916
1998 Impact Factor 0.645
1997 Impact Factor 0.748
1996 Impact Factor 0.618
1995 Impact Factor 0.531
1994 Impact Factor 0.64
1993 Impact Factor 0.619
1992 Impact Factor 0.623

Impact factor over time

Impact factor

Additional details

5-year impact 0.00
Cited half-life 8.60
Immediacy index 0.46
Eigenfactor 0.01
Article influence 0.60
Other titles Comparative biochemistry and physiology., Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Comparative biochemistry and physiology., Molecular and integrative physiology, Molecular & integrative physiology, Comp. biochem. physiol., CBP., Comparative biochemistry and physiology
ISSN 1531-4332
OCLC 41929819
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details


  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Pre-print allowed on any website or open access repository
    • Voluntary deposit by author of authors post-print allowed on authors' personal website, or institutions open scholarly website including Institutional Repository, without embargo, where there is not a policy or mandate
    • Deposit due to Funding Body, Institutional and Governmental policy or mandate only allowed where separate agreement between repository and the publisher exists.
    • Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months .
    • Set statement to accompany deposit
    • Published source must be acknowledged
    • Must link to journal home page or articles' DOI
    • Publisher's version/PDF cannot be used
    • Articles in some journals can be made Open Access on payment of additional charge
    • NIH Authors articles will be submitted to PubMed Central after 12 months
    • Publisher last contacted on 18/10/2013
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Studies of memory formation have recently concentrated on the possible role of N-methyl-d-aspartate receptors (NRs). We examined changes in the expression of three NRs (NR1, NR2B, and NR2C), olfactory receptor (OR), and adrenocorticotropic hormone (ACTH) in chum salmon (Oncorhynchus keta) using quantitative polymerase chain reaction (QPCR) during salinity change (seawater→50% seawater→freshwater). NRs significantly detected in the diencephalon and telencephalon and OR significantly detected in the olfactory epithelium. The expression of NRs, OR, and ACTH increased after the transition to freshwater. We also determined that treatment with MK-801, an antagonist of NRs, decreased NRs in telencephalon cells. In addition, a reduction in salinity was associated with increased levels of dopamine, ACTH, and cortisol (in vivo). Reductions in salinity evidently caused NRs and OR to increase the expression of cortisol and dopamine. We concluded that memory capacity and olfactory imprinting of salmon is related to the salinity of the environment during the migration to spawning sites. Furthermore, salinity affects the memory/imprinting and olfactory abilities, and cortisol and dopamine is also related with olfactory-related memories during migration. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; DOI:10.1016/j.cbpa.2015.04.011
  • [Show abstract] [Hide abstract]
    ABSTRACT: The hyperiid amphipod Phronima sedentaria experiences a temperature change of 15°C during diel migration in the Eastern Tropical North Pacific (ETNP) from 8-10°C at depth to 25-27°C at night in the surface waters. The aim of this study was to determine if the natural temperature gradient experienced by Phronima sedentaria results in a thermal stress response. Individuals were initially exposed to their night time temperatures (23°C) and subsequently subjected to temperatures within and above the range they typically experience. In the Eastern Tropical North Pacific P. sedentaria tolerates its normal night-time temperature (~23°C), but only for the duration of its stay there (~9 hours). Longer exposures (24 hours) result in elevated heat shock protein (hsp) expression. 29°C results in hsp expression, increased lactate production and 50% mortality at all exposure durations. This represents an upper critical temperature. Understanding the adaptations of pelagic amphipods to their current environment will help predict the physiological impacts of global warming for amphipods and their predators. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; DOI:10.1016/j.cbpa.2015.04.008
  • [Show abstract] [Hide abstract]
    ABSTRACT: Differences between species in the oxygen (O2) affinity (P50) of myoglobin (Mb) may serve to fine tune O2 supply to cardiac and skeletal muscle in ectotherms. In support of this view, it has been shown that fish Mb O2 affinities differ between species when measured at the same temperature, but are in fact similar when adjusted for in vivo muscle temperatures, most likely to maintain intracellular O2 delivery in species adapted to different environments. It is unknown whether similar adaptations exist in the O2 affinity of Mb from reptiles, despite this group of ectothermic vertebrates displaying great variation in the tolerance to both temperature and hypoxia. In this study, we have purified Mb from muscle tissues of three reptilian species (turtle, tortoise and alligator) with different lifestyles. We have measured O2 binding characteristics and autoxidation rates of the three Mbs and measured the effects of temperature, lactate and blocking of reactive thiols on the O2 affinity of turtle Mb. Our data show that, at a constant temperature, reptilian Mbs have similar O2 affinities that are lower than those of mammalian Mbs, which may optimize intracellular O2 transport at lower body temperatures. Reptilian Mbs have lower autoxidation rates than both mammalian and fish Mbs, which may be beneficial during oxidative stress. Furthermore, the O2 affinity of turtle Mb is without allosteric control and independent of either lactate or thiol covalent modification. This study reveals some common adaptive patterns in the temperature-dependent regulation of Mb oxygenation in vertebrates. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; DOI:10.1016/j.cbpa.2015.04.009
  • [Show abstract] [Hide abstract]
    ABSTRACT: The field of comparative physiology has a rich history of elegantly examining the effects of individual environmental factors on performance traits linked to fitness (e.g., thermal performance curves for locomotion). However, animals live in complex environments wherein multiple environmental factors vary simultaneously. Thus, we investigated the independent and interactive effects of temperature and energy intake on the growth and metabolic rate of juvenile corn snakes (Pantherophis guttatus) in the context of shifts in complex environments. Unlike previous studies that imposed constant or fluctuating temperature regimes, we manipulated the availability of preferred thermal microclimates (control vs. relatively warm regimes) for eight weeks and allowed snakes to behaviorally thermoregulate among microclimates. By also controlling for energy intake, we demonstrate an interactive effect of temperature and energy on growth-relevant temperature shifts had no effect on snakes' growth when energy intake was low and a positive effect on growth when energy intake was high. Thus, acclimation to relatively warm thermal options can result in increased rates of growth when food is abundant in a taxon in which body size confers fitness advantages. Temperature and energy also interactively influenced metabolic rate-snakes in the warmer temperature regime exhibited reduced metabolic rate (O2 consumption rate at 25°C and 30°C) if they had relatively high energy intake. Although we advocate for continued investigation into the effects of complex environments on other traits, our results indicate that warming may actually benefit important life history traits in some taxa and that metabolic shifts may underlie thermal acclimation. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; 187. DOI:10.1016/j.cbpa.2015.04.007
  • [Show abstract] [Hide abstract]
    ABSTRACT: Beta3-adrenergic receptors (AR) are important in teleost cardiovascular regulation. To date, it is unknown whether temperature acclimation changes ß3-AR functionality and consequently the involvement of this AR subtype in teleost cardiac regulation. Common carp (Cyprinus carpio) were acclimated at 12 °C or 23 °C (minimum 3 weeks) after which cardiovascular variables (cardiac output (Q), stroke volume (Sv) and heart rate (fH)) were measured upon injection of the ß3-AR agonist, BRL37344, and antagonist, SR59230A. In both 12 °C and 23 °C acclimated carp, BRL37344 induced significant increases in fH and Q whereas Sv was significantly decreased. While temperature did not affect the change (increase vs. decrease) in cardiac variables, the magnitude and on-set of responses differed. For instance, fH, Sv and Q responded significantly faster to ß3-AR stimulation in 23 °C carp. In contrast, maximum responses of fH and Q were significantly higher in 23 °C carp whereas the maximum response of Sv was significantly greater in 12 °C carp. These findings suggest that temperature acclimation induced changes in β3-AR receptor functionality (e.g. density and/or affinity). Stimulation of β3-ARs in 23 °C acclimated channel catfish (Ictalurus punctatus) caused significant increases in fH, Sv and Q. The increase in Sv was opposite to the decrease observed in 23 °C acclimated common carp. SR59230A induced significant decreases in Sv and Q but had no effect on these variables in carp (23 °C). Results suggest species diversity in the density and affinity or structure of ß3-ARs which may explain the different cardiac responses to ß3-AR ligands. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; 185. DOI:10.1016/j.cbpa.2015.04.005
  • [Show abstract] [Hide abstract]
    ABSTRACT: While there is a considerable body of work describing osmoregulation of elasmobranchs in brackish and saltwater, far fewer studies have investigated osmoregulation in hypersaline waters. We examined osmo- and ionoregulatory function and plasticity in juvenile brown-banded bamboo sharks, Chiloscyllium punctatum, exposed to three experimental salinities (25, 34 and 40 ‰) for two weeks. C. punctatum inhabits sheltered coastal areas and bays which can naturally become hypersaline as a consequence of evaporation of water but can also become hyposaline conditions during flood events. We hypothesised that C. punctatum would demonstrate a phenotypically plastic osmoregulatory physiology. Plasma osmolality, urea, Na(+) and Cl(-) levels increased significantly with increasing environmental salinity. Rectal gland and branchial sodium-potassium ATPase (NKA) activities were unaffected by salinity. Using immunohistochemistry and western blotting we found evidence for the presence of the key ion-regulatory proteins vacuolar H(+)-ATPase (VHA), pendrin (Cl(-)/HCO3(-) co-transporter) and the Na(+)-H(+) exchanger isoform 3 (NHE3) in discrete cells within the branchial epithelia. These results indicate that C. punctatum is a partially euryhaline elasmobranch able to maintain osmo- and ionoregulatory function between environmental salinities of 25 ‰ and 40 ‰. As suggested for other elasmobranchs, the gills of C. punctatum likely play a limited role in maintaining Na(+) homeostasis over the salinity range studied, but may play an important role in acid-base balance. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; 185. DOI:10.1016/j.cbpa.2015.04.001
  • [Show abstract] [Hide abstract]
    ABSTRACT: The euryhaline pupfish, Cyprinodon variegatus variegatus (Cvv), can osmoregulate in near freshwater conditions (2 mM Na(+)) and a freshwater population (C. v. hubbsi; Cvh) successfully osmoregulates at 0.1 mM Na(+). We previously demonstrated Cvv relies on an apical NKCC and NHE in the gill for Na(+) uptake in high (7 mM) and intermediate (2 mM) Na(+) concentrations. In contrast, Cvh relies on one or more NHEs for Na(+) uptake. This study investigated whether differences in NHE isoform use by Cvv and Cvh explain observed differences in Na(+) uptake kinetics between the two populations. We further studied whether Cvh uses an NHE-Rh metabolon or carbonic anhydrase (CA) to overcome thermodynamic challenges of NHE function in dilute freshwater. Transfer to more dilute freshwater resulted in upregulation of nhe-2 (Cvv only) and nhe-3 (Cvv and Cvh). The relative expression of nhe-3 compared to nhe-2 was only 2-fold higher in Cvv, but 200-fold higher in Cvh suggesting differential expression of nhe-3 is an important adaptation to freshwater in Cvh. Simultaneous measurement of Na(+) and Tamm flux under various conditions provided no support for the presence of a NHE-Rh metabolon in either population. Carbonic anhydrase appears to play an important role in Na(+) uptake. CA activity in Cvv was comparable in fish acclimated to 7 and 2 mM Na(+), but exposure to ethoxzolamide (CA inhibitor) stimulated Na(+) uptake by 91% and reduced Na(+) uptake by 58%, respectively. In Cvh acclimated to 7 mM Na(+), CA plays a relatively minor role in NHE function with exposure to ethoxzolamide inhibiting Na(+) uptake by 19%. However, at 0.1 mM Na(+), CA activity increased by 75% compared to 7 mM Na(+) fish and Na(+) uptake was inhibited by 31% after treatment with ethoxzolamide. Overall, our data suggests CA plays important, but different roles in regulating Na(+) uptake in both high Na(+) (Cvv) and low Na(+) (Cvh) freshwater. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; 185. DOI:10.1016/j.cbpa.2015.04.002
  • [Show abstract] [Hide abstract]
    ABSTRACT: The expression of uncoupling protein (UCP1) is up-regulated in mammalian brown adipocytes during cold exposure. However, a previous study revealed that UCP1 was highly expressed in the liver of common carps, and that the hepatic expression of UCP1 was down-regulated during cold exposure. The present study examined the effects of temperature on the recovery of UCP1 expression levels and the expression of genes involved in UCP1 transcription in the livers and kidneys of common carps. The hepatic and renal expression of UCP1 was decreased by acclimation from 22°C to 8°C, and a subsequent increase in the water temperature from 8°C to 28°C recovered the renal, but not hepatic expression of UCP1. Changes in the expression of peroxisome proliferator-activator receptor (PPAR) γ, retinoid X receptor (RXR) α and PPARγ co-activator (PGC)-1α, genes that are involved in the expression of UCP1 in mammals, with ambient temperature indicated that the expressions of PPARγ and RXRα, but not expression of PGC-1α was decreased in response to cold exposure; the hepatic and renal expressions of PPARγ and RXRα recovered to basal levels with the cessation of cold exposure, although this was not complete for hepatic expression of PPARγ. Cold exposure increased the expressions of PPARβ/δ and NRF1 both in the liver and kidney, as well as renal PGC-1α expression, and subsequent increases in the water temperature from 8°C to 28°C decreased these expression levels. The results of the present study indicate that a unique regulatory mechanism is responsible for the hepatic and renal expression of carp UCP1 during cold exposure and subsequent reacclimation, and is distinct from that in murine brown adipocytes. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 04/2015; 185. DOI:10.1016/j.cbpa.2015.04.003