Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Publisher: Elsevier

Journal description

Current impact factor: 2.37

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 2.371
2012 Impact Factor 2.167
2011 Impact Factor 2.235
2010 Impact Factor 2.134
2009 Impact Factor 2.196
2008 Impact Factor 1.709
2007 Impact Factor 1.863
2006 Impact Factor 1.553
2005 Impact Factor 1.351
2004 Impact Factor 1.635
2003 Impact Factor 1.556
2002 Impact Factor 1.274
2001 Impact Factor 1.026
2000 Impact Factor 0.883
1999 Impact Factor 0.916
1998 Impact Factor 0.645
1997 Impact Factor 0.748
1996 Impact Factor 0.618
1995 Impact Factor 0.531
1994 Impact Factor 0.64
1993 Impact Factor 0.619
1992 Impact Factor 0.623

Impact factor over time

Impact factor

Additional details

5-year impact 0.00
Cited half-life 8.60
Immediacy index 0.46
Eigenfactor 0.01
Article influence 0.60
Other titles Comparative biochemistry and physiology., Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Comparative biochemistry and physiology., Molecular and integrative physiology, Molecular & integrative physiology, Comp. biochem. physiol., CBP., Comparative biochemistry and physiology
ISSN 1531-4332
OCLC 41929819
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details


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  • Conditions
    • Authors pre-print on any website, including arXiv and RePEC
    • Author's post-print on author's personal website immediately
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    • Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months
    • Author's post-print may be used to update arXiv and RepEC
    • Publisher's version/PDF cannot be used
    • Must link to publisher version with DOI
    • Author's post-print must be released with a Creative Commons Attribution Non-Commercial No Derivatives License
    • Publisher last reviewed on 03/06/2015
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Prolonged and remote measurement of body temperature (Tb) in undisturbed small hibernators was not possible in the past because of technological limitations. Although passive integrated trandponders (PITs) have been used previously to measure subcutaneous temperature (Tsub) during daily torpor in a small marsupial, no study has attempted to use these devices at Tbs below 10°C. Therefore, we investigated whether subcutaneous interscapular PITs can be used as a viable tool for measuring Tb in a small hibernating bat (Nyctophilus gouldi; Ng) and compared it with measurements of Tb during daily torpor in a heterothermic bat (Syconycteris australis; Sa). The precision of transponders was investigated as a function of ambient temperature (Ta) and remote Tsub readings enabled us to quantify Tsub-Tb differentials during steady-state torpor and arousal. Transponders functioned well outside the manufacturers recommended range, down to ~5°C. At rest, Tsub and rectal Tb (Trec) were strongly correlated for both bat species (Ng r(2)=0.88; Sa r(2)=0.95) and this was also true for N. gouldi in steady-state torpor (r(2)=0.93). During induced rewarming from torpor Tsub increased faster than Trec In both species of bat. Our results demonstrate that transponders can be used to provide accurate remote measurement of Tb in two species of bats during different physiological states, both during steady-state conditions and throughout dynamic phases such as rewarming from torpor. We show that, at least during rewarming, regional heterothermy common to larger hibernators and other hibernating bats is also present in bats capable of daily torpor. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.08.007
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    ABSTRACT: Selection of broiler chickens for growth has led to increased adipose tissue accretion. To investigate the post-hatch development of adipose tissue, the abdominal, clavicular, and subcutaneous adipose tissue depots were collected from broiler chicks at 4 and 14days post-hatch. As a percent of body weight, abdominal fat increased (P<0.001) with age. At day 4, clavicular and subcutaneous fat depots were heavier (P<0.003) than abdominal fat whereas at day 14, abdominal and clavicular weighed more (P<0.003) than subcutaneous fat. Adipocyte area and diameter were greater in clavicular and subcutaneous than abdominal fat at 4 and 14days post-hatch (P<0.001). Glycerol-3-phosphate dehydrogenase (G3PDH) activity increased (P<0.001) in all depots from day 4 to 14, and at both ages was greatest in subcutaneous, intermediate in clavicular, and lowest in abdominal fat (P<0.05). In clavicular fat, peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein (CEBP)α, CEBPβ, fatty acid synthase (FASN), fatty acid binding protein 4 (FABP4), lipoprotein lipase (LPL), neuropeptide Y (NPY), and NPY receptor 5 (NPYR5) mRNA increased and NPYR2 mRNA decreased from day 4 to 14 (P<0.001). Thus, there are site-specific differences in broiler chick adipose development, with larger adipocytes and greater G3PDH activity in subcutaneous fat at day 4, more rapid growth of abdominal fat, and clavicular fat intermediate for most traits. Adipose tissue expansion was accompanied by changes in gene expression of adipose-associated factors. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.08.002
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    ABSTRACT: We studied the physiological and behavioral responses of the Ponto-Caspian amphipod Dikerogammarus villosus during exposure to four total ammonia concentrations (NH3+NH4(+); TotAmm): 0.003 (control), 0.06, 1.6 and 7.0 mmol L(-1) (0.042, 0.84, 22.4 and 98.0 mg L(-1)) for a period of up to 12 h at 21°C. During the transition period from the control to treatment concentration as well as during the first hour of exposure to 0.06 and 1.6 mmol L(-1), gammarids increased their locomotor activity which was manifested in significantly higher routine metabolic rates compared to control conditions. At control conditions the resting metabolic rate amounted to 0.98±0.26 mW g(-1), and significantly increased by 19 and 37% at 0.06 and 1.6 mmol L(-1), respectively. The highest examined [TotAmm] caused a rapid and significant increase in resting metabolic rate by 37% within the first four hours of exposure before gammarids died. The exposure to elevated [TotAmm] also resulted in a significant decreased RNA:DNA ratio and significantly higher glycogen concentrations compared to the control. We conclude that even a short exposure to [TotAmm] of 0.06 mmol L(-1), which may occur in natural habitats, disturbs the physiology and behavior of D. villosus and leads to increased metabolic costs of the maintenance and reduced protein synthesis. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.08.003
  • [Show abstract] [Hide abstract]
    ABSTRACT: Chickens selected for low (LWS) and high (HWS) juvenile body weight (BW) for 55 generations differ in BW by 10-fold at selection age. High (HWR) and low (LWR) body weight-relaxed lines have been random-bred since the 46th generation. Our objective was to evaluate the developmental and nutritional regulation of pancreatic mRNA abundance of pancreatic and duodenal homeobox 1 (PDX1), preproinsulin (PPI), preproglucagon (PPG), and glucose transporter 2 (GLUT2). At day of hatch (DOH) and days 1, 3, 7, and 15 (D1, 3, 7 and 15, respectively), pancreas was collected and real time PCR was performed in Experiment 1. In Experiment 2, HWS and LWS were fed or delayed access to food for 72 hours post-hatch, and pancreas collected at D15. There was an interaction of line and age for GLUT2 (P = 0.001), PPI (P < 0.0001), PPG (P = 0.034), and PDX1 (P < 0.0001). Expression was greater in chicks from LWR and LWS than HWR and HWS. There was an interaction of line and nutrition on PPG (P < 0.0001) and GLUT2 (P = 0.001) mRNA, where expression was similar among chicks that were fed but greater in LWS than HWS when chicks were delayed access to food. Thus, the first two weeks is important for maturation of pancreatic endocrine function. Long-term selection for BW is associated with differences in pancreas development, and delaying access to food at hatch may have persisting effects on glucose regulatory function. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.08.004
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    ABSTRACT: In the present study, treatment of Xenopus laevis A6 kidney epithelial cells with the proteasomal inhibitor, MG132 or the environmental toxicants, sodium arsenite or cadmium chloride, induced the accumulation of the small heat shock protein, HSP30, in total and in both soluble and insoluble protein fractions. Immunocytochemical analysis revealed the presence of relatively large HSP30 structures primarily in the perinuclear region of the cytoplasm. All three of the stressors promoted the formation of aggresome-like inclusion bodies as determined by immunocytochemistry and laser scanning confocal microscopy using a ProteoStat aggresome dye and additional aggresomal markers, namely, anti-γ-tubulin and anti-vimentin antibodies. Further analysis revealed that HSP30 co-localized with these aggresome-like inclusion bodies. In most cells, HSP30 was found to envelope or occur within these structures. Finally, we show that treatment of cells with withaferin A, a steroidal lactone with anti-inflammatory, anti-tumour and proteasomal inhibitor properties, also induced HSP30 accumulation that co-localized with aggresome-like inclusion bodies. It is possible that proteasomal inhibitor or metal/metalloid-induced formation of aggresome-like inclusion bodies may sequester toxic protein aggregates until they can be degraded. While the role of HSP30 in these aggresome-like structures is not known, it is possible that they may be involved in various aspects of aggresome-like inclusion body formation or transport. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.07.022
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    ABSTRACT: In the face of a changing climate, questions regarding sub-lethal effects of elevated habitat temperature on the physiology of ectotherms remain unanswered. In particular, long-term responses of ectotherms to the warming trend in tropical regions are unknown and significantly understudied due primarily to the difficulties in specimen and community traceability. In freshwater lakes employed as cooling reservoirs for power plants, increased physiological stress from high water temperature can lead to an increase in mortality, reduce growth, and potentially alter the community structure of fishes. Throughout this study, we employ this highly tractable system to assess how elevated thermal regimes can alter the physiology and consequently the ecology of aquatic species. We documented a significantly reduced lifespan, growth performance, and a shift in the age structure toward younger individuals in the thermally impacted population of bluegill (Lepomis macrochirus) in Coffeen Lake in Illinois, compared to a non-impacted control group (Lake Mattoon). Average age calculated for the Lake Mattoon population was 2.42years, whereas the average age of bluegill from Coffeen Lake was only 0.96years. The average specimen mass in Lake Mattoon was more than six times that of Coffeen Lake average (Mattoon=60.26g; Coffeen=9.42g). During laboratory cross-acclimation studies of bluegill from Lake Mattoon at 17.5 and 35.0°C, citrate synthase activity obtained from white muscle was regulated through acclimation, whereas cold-acclimated specimens exhibited twice the activity at 25°C, if compared to CS activity values from warm-acclimated specimens. This study raises the questions about the causal relationships between physiological performance and habitat temperature, in particular how thresholds in an organism's physiology may modulate their community structure, and consequently their ecological success. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.07.014
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    ABSTRACT: Seawater acclimation is a critical period for anadromous species and a process yet to be understood in lampreys. Considering that changes in lipid composition of the gill cells´ basolateral membranes may disrupt the major transporter Na(+)K(+)-ATPase, this study goal was to detect changes at this level during juvenile sea lamprey seawater acclimation. The results showed that saltwater acclimation has a direct effect on the fatty acid composition of gill cells basolateral membrane´s phospholipids. When held in full-strength seawater, the fatty acids profile of basolateral membrane´s phospholipids suffered a restructure by increasing either saturation or the ratio between oleic acid and eicosapentaenoic acid. Simultaneously, the activity of Na(+)K(+)-ATPase revealed a significant and positive correlation with basolateral membrane´s cholesterol content in presence of highest salinity. Our results pointed out for lipid adjustments involving the functional transporter present on the gill cell basolateral membranes to ensure the role played by branchial Na(+)K(+)-ATPase in ion transport during saltwater acclimation process. The responses observed contributed to the strategy adopted by gill cell´s basolateral membranes to compensate for osmotic and ionic stressors, to ensure the success of the process of seawater acclimation associated with the downstream trophic migration of juvenile sea lamprey. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.07.018
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    ABSTRACT: According to the rate of living-free radical hypothesis, higher metabolic rates should increase reactive oxygen species (ROS) production. However, the "uncoupling to survive" hypothesis postulates that uncoupling proteins (UCPs) can decrease ROS production by lowering the potential of the inner mitochondrial membrane, in which case the correlation between metabolic rate and ROS levels would be a negative rather than positive. In this study, we examined energy intake, oxidative stress levels, antioxidant activity and the expression of UCPs in brown adipose tissue (BAT), and in the liver, heart, skeletal muscle and brain, of striped hamsters (Cricetulus barabensis) acclimated to either 5°C or 32.5°C. The energy intake of hamsters acclimated to 5°C increased by 70.7%, whereas the energy intake of hamsters acclimated to 32.5°C decreased by 31.3%, relative to hamsters kept at room temperature (21°C) (P<0.05). Malonadialdehyde (MDA) levels, total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-PX) activity in BAT significantly decreased in the hamsters acclimated to 5°C, but increased in the hamsters acclimated to 32.5°C, relative to those at 21°C. Neither ROS levels (i.e. H2O2 levels), nor antioxidants in skeletal muscle, liver, heart or brain tissue, were affected by temperature. UCP1 expression in BAT was significantly up-regulated in 5°C group, but down-regulated in 32.5°C group, relative to the 21°C group. UCP3 expression of skeletal muscle was also up-regulated significantly in hamsters acclimated to 5°C. These results suggest that the relationship between ROS levels and metabolic rate was negative, rather than positive. UCP1 expression in BAT may have played a role in lowering ROS levels. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.07.017
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    ABSTRACT: Basal metabolic rate (BMR) is an intensively topic studied in ecophysiology for the purpose of understanding energy budgets of the species, variations of energy expenditure during their diary activities and physiological acclimatization to the environment. Establishing how the metabolism is assembled to the environment can provide valuable data to improve conservation strategies of endangered species. In this sense, metabolic differences associated to habitats have been widely reported in the interspecific level, however little is known about the intraspecific view of BMR under an environmental gradient. In this study, we researched the effect of the habitat on metabolic rate of an Iberian endemic species: Iberomys cabrerae. Animals were captured in different subpopulations of its altitudinal range and their MR was studied over a thermal gradient. MR was analyzed through a Linear Mixed Models (LMM) in which, in addition to thermal effects, the bioclimatic zone and sex also influenced in the metabolism of the species. The beginning of thermoneutrality zone was set on 26.5° C and RMR was 2.3ml O2·g(-1)·h(-1), intermediate between both bioclimatic zones. Supramediterranean subpopulations started the Tlc earlier (24.9° C) and had higher RMR than the mesomediterranean ones (26.9° C). The thermal environment together with primary productivity conditions could explain this difference in the metabolic behaviour of the Cabrera voles. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 08/2015; DOI:10.1016/j.cbpa.2015.08.011
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    ABSTRACT: Few investigations have studied digestive enzyme activities in the alimentary tracts of sharks to gain insight into how these organisms digest their meals. In this study, we examined the activity levels of proteases, carbohydrases, and lipase in the pancreas, and along the anterior intestine, spiral intestine, and colon of the bonnethead shark, Sphyrna tiburo. We then interpreted our data in the context of a rate-yield continuum to discern this shark's digestive strategy. Our data show anticipated decreasing patterns in the activities of pancreatic enzymes moving posteriorly along the gut, but also show mid-spiral intestine peaks in aminopeptidase and lipase activities, which support the spiral intestine as the main site of absorption in bonnetheads. Interestingly, we observed spikes in the activity levels of N-acetyl-β-d-glucosaminidase and β-glucosidase in the bonnethead colon, and these chitin and cellulose, respectively, degrading enzymes are likely of microbial origin in this distal gut region. Taken in the context of intake and relatively long transit times of food through the gut, the colonic spikes in N-acetyl-β-d-glucosaminidase and β-glucosidase activities support the contention that bonnetheads take a yield-maximizing strategy to the digestive process, with some reliance on microbial digestion in their hindguts. This is one of the first studies to examine digestive enzyme activities along the gut of any shark, and importantly, the data match with previous observations that sharks take an extended time to digest their meals (consistent with a yield-maximizing digestive strategy), and that the spiral intestine is the primary site of absorption in sharks. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 07/2015; DOI:10.1016/j.cbpa.2015.07.013
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    ABSTRACT: Gill epithelium permeability and qualitative/quantitative aspects of gill claudin (cldn) tight junction (TJ) protein transcriptomics were examined using a primary cultured model gill epithelium developed using the euryhaline puffer fish Tetraodon nigroviridis. The gill model was prepared using seawater-acclimated fish and is cultured on permeable cell culture filter supports. It is composed of 1-2 overlapping confluent layers of gill pavement cells (PVCs), with the outer layer exhibiting prominent apical surface microridges and TJs between adjacent cells. During the development of electrophysiological characteristics, the model exhibits a sigmoidal increase in transpithelial resistance (TER) and plateaus around 30 kΩcm(2). At this point paracellular movement of [(3)H]polyethylene glycol (PEG) 4000 is low at ~ 1.75 cm s(-1) x 10(-7). When exposed to apical seawater (SW) epithelia exhibit a marked decrease in TER while PEG flux remains unchanged for at least 6h. In association with this, transcript encoding cldn TJ proteins cldn3c, -23b, -27a, -27c, -32a and -33b increased during the first 6h while cldn11a decreased. This suggests that these proteins are involved in maintaining barrier properties between gill PVCs of SW fishes. Gill cldn mRNA abundance was also altered 6 and 12 h following abrupt SW exposure of puffer fish, but in a qualitatively and quantitatively different manner from the cultured model epithelium. This most likely reflects the cellular heterogeneity of whole tissue and/or the contribution of the endocrine system in intact fish. The current study provides insight into the physiological and transcriptomic response of euryhaline fish gill cells to a hyperosmotic environment. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 07/2015; DOI:10.1016/j.cbpa.2015.07.015
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    ABSTRACT: Coral reefs are an indispensible worldwide resource, accounting for billions of dollars in cultural, economic, and ecological services. An understanding of coral reproduction is essential to determining the effects of environmental stressors on coral reef ecosystems and their persistence into the future. Here we describe the presence of and changes in steroidal hormones along with associated steroidogenic and steroid removal enzymes during the reproductive cycle of the brooding, pan-Pacific, hermaphroditic coral, Pocillopora damicornis. Detectable levels of 17β-estradiol, estrone, progesterone and testosterone were consistently detected over two consecutive lunar reproductive cycles in coral tissue. Intra-colony variation in steroid hormone levels ranged between 1.5 and 2.2 fold and were not statistically different. Activities of the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase and cytochrome P450 (CYP) 17 dehydrogenase were detectable and did not fluctuate over the reproductive cycle. Aromatase-like activity was detected during the lunar reproductive cycle with no significant fluctuations. Activities of regeneration enzymes did not fluctuate over the lunar cycle; however, activity of the clearance enzyme UDP-glucuronosyl transferases increased significantly (ANOVA, post hoc p<0.01) during the two weeks before and after peak larval release (planulation), suggesting activity of this enzyme family may be linked to the reproductive state of the coral. Sulfotransferase enzymes could not be detected. Our findings provide the first data defining normal physiological and lunar/reproductive variability in steroidal enzymes in a coral species with respect to their potential role in coral reproduction. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 07/2015; DOI:10.1016/j.cbpa.2015.07.012
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    ABSTRACT: Phenotypic plasticity may buffer the selection pressures on organisms that inhabit novel or rapidly-changing environments. We investigated plasticity of thermal tolerance, energetic and water loss traits and their interaction with behaviour in a small-bodied, arboreal anuran (Hyperolius marmoratus Rapp, Hyperoliidae) undergoing rapid range expansion in the winter rainfall region of South Africa. After short-term exposure to three temperatures (acclimation treatments) commonly encountered in their historical and novel ranges, frogs exhibited a broad thermal tolerance range (mean±s.d.: 42.1±2.9°C) and higher plasticity in CTmax than in CTmin. Resting metabolic rate was lowest in cold-acclimated animals, while active metabolic rates were lowest in warm-acclimated frogs, likely reflecting compensation towards energy conservation. Evaporative water loss was not significantly altered by the acclimation treatments in either resting or active animals, indicating limited plasticity in this trait compared to metabolism. Our results suggest that plasticity of temperature limits and metabolism may benefit this species in variable environments such as those encountered in its expanded range. Lack of plasticity in water loss during resting and activity suggests that these frogs rely on their high cutaneous resistance and behavioural means to buffer climate variation. This study highlights the importance of synergistic interactions between physiology and behaviour in determining amphibian responses to temperature variation. Copyright © 2015. Published by Elsevier Inc.
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 07/2015; DOI:10.1016/j.cbpa.2015.06.033