Electrophoresis (Electrophoresis)

Publisher: Electrophoresis Society; International Electrophoresis Society, Wiley-VCH Verlag

Journal description

Attracting high-quality papers from the world's leading laboratories ELECTROPHORESIS has proved itself indispensable in the life sciences where electrophoresis is the most widely used method. ELECTROPHORESIS is the foremost journal for new analytical and preparative methods and for innovative applications on all aspects of electrophoresis. It has an international scope and publishes original papers short communications and review articles in the following areas: biochemistry molecular and cell biochemistry genetics immunology microbiology clinical chemistry forensics food science and many more. Special Issues Paper Symposium issues devoted to new developments prepared by an expert Guest Editor are published regularly with special emphasis on capillary electrophoresis (2-3 issues per year)and proteomics. The journal also publishes proceedings of international and national electrophoresis meetings.

Current impact factor: 3.03

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 3.028
2013 Impact Factor 3.161
2012 Impact Factor 3.261
2011 Impact Factor 3.303
2010 Impact Factor 3.569
2009 Impact Factor 3.077
2008 Impact Factor 3.509
2007 Impact Factor 3.609
2006 Impact Factor 4.101
2005 Impact Factor 3.85
2004 Impact Factor 3.743
2003 Impact Factor 4.04
2002 Impact Factor 4.325
2001 Impact Factor 4.282
2000 Impact Factor 3.385
1999 Impact Factor 3.447
1998 Impact Factor 3.054
1997 Impact Factor 2.848
1996 Impact Factor 2.467
1995 Impact Factor 2.73
1994 Impact Factor 2.274
1993 Impact Factor 1.842
1992 Impact Factor 2.159

Impact factor over time

Impact factor

Additional details

5-year impact 2.72
Cited half-life 7.90
Immediacy index 0.53
Eigenfactor 0.02
Article influence 0.58
Website Electrophoresis website
Other titles Electrophoresis (Online), Electrophoresis, Proteomics reviews
ISSN 1522-2683
OCLC 43388561
Material type Periodical, Program, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Wiley-VCH Verlag

  • Pre-print
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  • Post-print
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  • Restrictions
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  • Conditions
    • Pre-print may be deposited on personal intranet or institutional intranet repository, but not on a public repository
    • Pre-print must not updates with future versions
    • Published source must be acknowledged with set phrases (See policy)
    • Must link to publisher's site: http://www.interscience.wiley.com/
    • Publisher's version/PDF cannot be used
    • Some journal exceptions-check individual homepages
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, N-linked glycans from intact, formalin treated and formalin fixed paraffin embedded (FFPE) standard glycoproteins, human serum and mouse tumor tissue samples were investigated in respect to their susceptibility for formaldehyde treatment mediated changes. FFPE samples were first deparaffinized, followed by solubilization in RIPA buffer and treated with PNGase F for N-glycan release. The released glycans were labeled with a charged fluorophore (APTS) and analyzed by capillary electrophoresis with laser induced fluorescent detection. No significant alterations were found in the N-glycome profile at any of the investigated complexation levels (i.e., glycoprotein, serum and tissue samples) of the study. These results suggest that FFPE samples can be readily used for global N-glycome analysis holding the promise to find novel carbohydrate biomarkers in prospective and retrospective studies. Exoglycosidase based carbohydrate sequencing was also applied to reveal some basic structural information about the N-linked carbohydrates of the mouse tumor tissue samples. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500446
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    ABSTRACT: Paper-based microfluidic fuel cells (MFCs) are a potential replacement for traditional FCs and batteries due to their low cost, portability, and simplicity to operate. In MFCs, separate solutions of fuel and oxidant migrate through paper due to capillary action and laminar flow and, upon contact with each other and catalyst, produce electricity. In the present work, we describe an improved microfluidic paper-based direct formate FC (DFFC) employing formate and hydrogen peroxide as the anode fuel and cathode oxidant, respectively. The dimensions of the lateral column, current collectors and cathode were optimized. A maximum power density of 2.53 mW/cm(2) was achieved with a DFFC of surface area 1.5 3.0 cm(2) , steel mesh as current collector, 5% carbon to paint mass ratio for cathode electrode and, 30% hydrogen peroxide. The longevity of the MFC's detailed herein is greater than eight hours with continuous flow of streams. In a series configuration, the MFCs generate sufficient energy to power light-emitting diodes (LEDs) and a handheld calculator. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500360
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    ABSTRACT: The demand for organic food is increasing annually due to the growing consumer trend for more natural products that have simpler ingredient lists, involve less processing and are grown free of pesticides. However, there is still not enough nutritional evidence in favor of organic food consumption. Classical chemical analysis of macro- and micronutrients has demonstrated that organic crops are poorer in nitrogen, but clear evidence for other nutrients is lacking. Omics technologies forming part of the new discipline of foodomics have allowed the detection of possible nutritional differences between organic and conventional production, although many results remain controversial and contradictory. The main focus of this review is to provide an overview of the studies that use foodomics techniques as a tool to differentiate between organic and conventional production. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500348
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    ABSTRACT: In this work, a [Cu(mal)(bpy)]·H2 O (mal = L-(-)-malic acid; bpy = 4,4'-bipyridyl) homochiral metal-organic framework (MOF) was synthesized and used to modify the inner walls of capillary columns by utilizing amido bonds to form covalent links between the MOF particles and the capillary inner wall. The synthesized [Cu(mal)(bpy)]·H2 O and MOF-modified capillary column were characterized by X-ray diffraction (XRD), thermogravimetric analysis (TGA), particle size distribution analysis, nitrogen absorption characterization, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The MOF-modified capillary column was used for the stereoisomer separation of some drugs. The limit of detection (LOD) and limit of quantitation (LOQ) of the 6 analytes were 0.1 μg/mL and 0.25 μg/mL, respectively. The linear range was 0.25-250 μg/mL for ephedrine, 0.25-250 μg/mL for pseudoephedrine, 0.25-180 μg/mL for d-penicillamine, 0.25-120 μg/mL for l-penicillamine, 0.25-180 μg/mL for d-phenylalanine and 0.25-160 μg/mL for l-phenylalanine, all with R(2) > 0.999. Finally, the MOF-modified capillary column was applied for the analysis of active ingredients in a real sample of the traditional Chinese medicine ephedra. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500342
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    ABSTRACT: We described a strategy to perform multi-step operations on a simple laminated paper-based separation device by using electrokinetic flow to manipulate the fluids. A laminated crossed-channel paper-based separation device was fabricated by cutting a filter paper sheet followed by lamination. Multiple function units including sample loading, sample injection and electrophoretic separation were integrated on a single paper-based analytical device for the first time, by applying potential at different reservoirs for sample, sample waste, buffer and buffer waste. As a proof-of-concept demonstration, mixed sample solution containing carmine and sunset yellow were loaded in the sampling channel, and then injected into separation channel followed by electrophoretic separation, by adjusting the potentials applied at the four terminals of sampling and separation channel. The effects of buffer pH, buffer concentration, channel width and separation time on resolution of electrophoretic separation were studied. This strategy may be used to perform multi-step operations such as reagent dilution, sample injection, mixing, reaction and separation on a single microfluidic paper-based analytical device, which is very attractive for building micro total analysis systems on microfluidic paper-based analytical devices. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500321
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    ABSTRACT: In this paper, an open tubular affinity capillary electrochromatography (OT-ACEC) was developed by physical adsorption of rabbit platelets on the inner surface of capillary. The interactions between small molecules include adenosine diphosphate (ADP) (positive control), protocatechuic acid (negative control) and seven natural products (salvianolic acid B, salvianic acid A sodium, hydroxysafflor yellow A, ferulic acid, chlorogenic acid, sinapic acid, caffeic acid) and platelets were evaluated by their retention factors and binding constants obtained based on peak-shift assay. Then, the activities of anti-platelet aggregation induced by thrombin (THR), ADP and arachidonic acid (AA) for those small molecules (except ADP) were evaluated by turbidimetric method. The results indicate that: 1) ADP, a platelet aggregation inducer, had strong interaction with platelet, while protocatechuic acid that had no inhibition on platelet aggregation behaved no specific interaction; 2) there was a positive correlation between the anti-platelet aggregation activities of small molecules and their interactions with platelet, generally those compounds with higher binding constants with platelet exhibited higher activities. Therefore, the OT-ACEC method developed in the present study can be a potential method to evaluate affinity interactions between small molecules and platelets, so as to predict the biological activities such as anti-platelet aggregation for the small molecules. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500414
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    ABSTRACT: Although the resolution of capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) has been significantly improved by using a poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO; Pluronic(®) ) triblock copolymer as a separation medium, CE-SSCP on a microchip format is not widely applicable because their resolution is limited by short channel length. Therefore, a strategy to improve the resolution in channels of limited lengths is highly required for enabling microchip-based CE-SSCP. In this study, we developed a high-resolution CE-SSCP microchip system by controlling the width of the Pluronic-filled channel. We tested four different channel widths of 180, 240, 300, and 400 μm, and found that 300 μm showed the highest resolution in the separation of two pathogen specific markers. Potential applications of our method in various genetic analyses were also shown by using single nucleotide polymorphism markers for spinal muscular atrophy. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500427
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    ABSTRACT: This work aims at studying the optimization of an on-line Capillary Electrophoresis (CE)-based tryptic digestion methodology for the analysis of therapeutic polypeptides (PP). With this methodology, a mixture of surrogate peptide fragments and amino acid were produced on-line by trypsin cleavage (enzymatic digestion) and subsequently analyzed using the same capillary. The resulting automation of all steps such as injection, mixing, incubation, separation and detection minimizes the possible errors and saves experimental time. In this paper, we first study the differents parameters influencing PP cleavage inside the capillary (plug length, reactant concentration, incubation time, diffusion and electrophoretic plugs mixing). In a second part, the optimization of the electrophoretic separation conditions of generated hydrolysis products (nature, pH and Ionic Strength (I) of the Background Electrolyte (BGE)) is described. Using the optimized conditions, excellent repeatability was obtained in terms of separation (migration times) and proteolysis (number of products from enzymatic hydrolysis and corresponding amounts) demonstrating the robustness of the proposed methodology. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500349
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    ABSTRACT: Polydimethylsiloxane (PDMS) and polymethyl methacrylate (PMMA) are two of the most used polymers in the fabrication of lab-on-chip or microfluidic devices. In order to use these polymers in biological applications, it is sometimes essential to be able to bind biomolecules such as proteins and DNA to the surface of these materials. In this work we have evaluated a number of processes that have been developed to bind protein to PDMS surfaces which include passive adsorption, passive adsorption with Glutaraldehyde cross-linking, APTES functionalization followed by Glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) (EDC) cross linkers. It has been shown that the latter technique -using EDC- results in more than twice the bonding of protein to the surface of PDMS microchannels than proteins binding passively. We have also evaluated a few techniques that have been tested for the functionalization of PMMA microchannels where we have found that the use of polyethylene imine (PEI) has led to the strongest protein-PMMA microchannel bond. We finally demonstrated the effect of PDMS curing methodology on protein adsorption to its surface, and showed that increased curing time is the factor that reduces passive adsorption the most. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500333
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    ABSTRACT: Surface properties of nanoparticle are of high importance in the field of biotechnology, drug delivery and micro/nanofabrication. In this article, we developed a comprehensive theoretical model and subsequently solved that numerically to study the effect of thermodiffusion of ions on surface charge properties of nanoparticle. The theoretical study has been done considering silica nanoparticle for two aqueous solutions NaCl and KCl. The effect of solution pH in conjunction with nanoparticle temperature on surface charge density has been obtained for different salt concentration (1, 10 and 100mM) and nanoparticle size (diameter of 2 and 100 nm). It is observed from the results that with increasing temperature of the nanoparticle, the negative surface charge density gets higher due to increasing thermodiffusion effect. It is also found out that the magnitude of surface charge density is higher for KCl solution than NaCl solution under same condition which is attributed mostly due to less thermodiffusion of counterions for KCl than NaCl. Present study also shows that magnitude of surface charge density decreases with increasing nanoparticle size until it reaches a limiting value (called critical size) above which the effect of nanoparticle size on surface charge density is insignificant. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500374
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    ABSTRACT: We have evaluated capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) for detection of trace amounts of host cell protein impurities in recombinant therapeutics. Compared to previously published procedures, we have optimized the buffer pH used in the formation of a pH junction to increase injection volume. We also prepared a five-point calibration curve by spiking twelve standard proteins into a solution of a human monoclonal antibody. A custom CZE-MS/MS system was used to analyze the tryptic digest of this mixture without depletion of the antibody. CZE generated a ∼70 min separation window (∼90 min total analysis duration) and ∼300 peak capacity. We also analyzed the sample using ultra-performance liquid chromatography (UPLC)-MS/MS. CZE-MS/MS generated ∼five times higher base peak intensity and more peptide identifications for low-level spiked proteins. Both methods detected all proteins spiked at the ∼100 ppm level with respect to the antibody. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500301
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    ABSTRACT: Precise measurement of nitric oxide (NO) is of great importance to understand the function of NO in liver and the mechanism of liver injury. 8-(3',4'-Diamino phenyl)-3,5-(2-hydroxyphenyl)-dimethylene pyrrole (BOPB), a fluorescent probe in the red region (> 600 nm) newly developed in our group, has good photo-stability and excitation/emission wavelength of 622/643 nm matching well with commercial 635 nm semiconductor laser of capillary electrophoresis with laser induced fluorescence detection (CE-LIF). Therefore, BOPB was used in CE-LIF for the determination of NO in mice liver. Both derivatization and separation conditions were optimized. Derivatization reaction of BOPB and NO was carried out in pH 7.4 PBS buffer at 35 ℃ for 12 min and the separation of NO derivative (BOPB-T) of BOPB was achieved within 7.0 min in pH 9.0 running buffer containing 15 mM H3 BO3 -NaOH and 15 mM SDS. Good linearity was found in the range of 1.0×10(-9) - 5.0×10(-7) M with the limit of detection of 0.02 nM. The proposed method was applied to the analysis of NO in real samples, and NO concentration was increased obviously in acute liver injury of mice. Compared to existing derivatization-based CE-LIF methods for NO, this method has lower LOD and less background interference owing to detection wavelength of BOPB in the red region. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500341
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    ABSTRACT: Herein, we report a technique for detecting the fast binding of antibody-peptide inside a capillary. Anti-HA was mixed and interacted with FAM labeled HA tag (FAM-E4 ) inside the capillary. Fluorescence coupled capillary electrophoresis (CE-FL) was employed to measure and record the binding process. The efficiency of the antibody-peptide binding on in-capillary assays was found to be affected by the molar ratio. Furthermore, the stability of anti-HA-FAM-E4 complex was investigated as well. The results indicated that E4 YPYDVPDYA (E4 ) or TAMRA-E4 YPYDVPDYA (TAMRA-E4 ) had the same binding priorities with anti-HA. The addition of excess E4 or TAMRA-E4 could lead to partial dissociation of the complex and take a two-step mechanism including dissociation and association. This method can be applied to detect a wide range of biomolecular interactions. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500429
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    ABSTRACT: This study describes a method to determine non-steroidal anti-inflammatory drugs in urine samples based on the use of single drop microextraction (SDME) in a three-phase design as a preconcentration technique coupled in-line to capillary electrophoresis. Different parameters affecting the extraction efficiency of the SDME process were evaluated (e.g. type of extractant, volume of the microdroplet and extraction time). The developed method was successfully applied to the analysis of human urine samples with LODs ranging between 1.0 and 2.5 μg/mL for all of the NSAIDs under study. This method shows RSD values ranging from 8.5 to 15.3 % in inter-day analysis. The enrichment factors were calculated, resulting 27-fold for ketoprofen, 14-fold for diclofenac, 12-fold for ibuprofen and 44-fold naproxen. Samples were analysed applying the SDME-CE method and the obtained results presented satisfactory recovery values (82-115%). The overall method can be considered a promising approach for the analysis of NSAIDs in urine samples after minimal sample pretreatment. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500373
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    ABSTRACT: In this work lab-made poly(dimethylsiloxane) (PDMS) microfluidic chips were matched to a capacitively coupled contactless conductivity detector (C(4) D) having external in-plane electrodes (eDAQ, Australia). The advantages of this type of C(4) D are the choice to reversibly place or remove the microchip onto/from the detector and to freely variate the position of the detection (separation length) on the microchip. The thickness of the bottom layer of the PDMS chip was optimized to achieve sensitive detection during the electrophoretic separation. PDMS chips with 100 μm bottom layer used with the C(4) D platform were tested by CZE of a mixture of seven anions and different types of real samples. Using split-flow pressure sample injection and effective length of 6.5 cm, the numbers of theoretical plates were in the range of 4 000-6 000 (63 000/m - 93 000/m) and the LODs amounted to 3.66 μmol/L-14.7 μmol/L (0.13-2.26 μg/mL) for the studied anions. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500335
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    ABSTRACT: The relative polarization behavior of micron and sub-micron polystyrene particles was investigated under direct current (DC) and very low frequency (<1 kHz) alternating current (AC) electric fields. Relative polarization of particles with respect to the suspending medium is expressed in terms of the Clausius-Mossotti factor, a parameter of crucial importance in dielectrophoretic-based operations. Particle relative polarization was studied by employing insulator-based dielectrophoretic (iDEP) devices. The effects of particle size, medium conductivity, and frequency (10-1000 Hz) of the applied electric potential on particle response were assessed through experiments and mathematical modeling with COMSOL Multiphysics. Particles of different sizes (100 - 1000 nm diameters) were introduced into iDEP devices fabricated from polydimethylsiloxane and particle dielectrophoretic responses under DC and AC electric fields were recorded and analyzed in the form of images and videos. The results illustrated that particle polarizability and dielectrophoretic response depend greatly on particle size and the frequency of the electric field. Smaller particles tend to exhibit positive DEP at higher frequencies (200-1000 Hz), while larger particles exhibit negative DEP at lower frequencies (20-200 Hz). These differences in relative polarization responses can be used for the design of iDEP-based separations and analysis of particle mixtures. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500338
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    ABSTRACT: Ribonuclease A (RNase A) has proven potential as a therapeutic agent, especially in its PEGylated form. Grafting of PEG molecules to this protein yields mono-PEGylated and di-PEGylated RNase A conjugates, and the unreacted protein. Mono-PEG RNase A is of great interest. The use of electrokinetic forces in microdevices represents a novel alternative to chromatographic methods to separate this specie. This work describes the dielectrophoretic behavior of the main protein products of the RNase A PEGylation inside a microchannel with insulators under DC electric fields. This approach represents the first step in route to design micro-bioprocesses to separate PEGylated RNase A from unreacted native protein. The three proteins exhibited different dielectrophoretic behaviors. All of them experienced a marked streaming pattern at 3000 V consistent with positive DEP. Native protein was not captured at any of the conditions tested, while mono and di-PEGylated RNase A were captured presumably due to positive DEP at 4000 and 2500 V, respectively. Concentration of mono-PEGylated RNase A with a maximal enrichment efficiency of ≈9.6 times the feed concentration was achieved in few seconds. These findings open the possibility of designing novel devices for rapid separation, concentration, and recovery of PEGylated RNase A in a one-step operation. This article is protected by copyright. All rights reserved.
    Electrophoresis 11/2015; DOI:10.1002/elps.201500311