Electrophoresis (Electrophoresis )

Publisher: Electrophoresis Society; International Electrophoresis Society, John Wiley & Sons

Description

Attracting high-quality papers from the world's leading laboratories ELECTROPHORESIS has proved itself indispensable in the life sciences where electrophoresis is the most widely used method. ELECTROPHORESIS is the foremost journal for new analytical and preparative methods and for innovative applications on all aspects of electrophoresis. It has an international scope and publishes original papers short communications and review articles in the following areas: biochemistry molecular and cell biochemistry genetics immunology microbiology clinical chemistry forensics food science and many more. Special Issues Paper Symposium issues devoted to new developments prepared by an expert Guest Editor are published regularly with special emphasis on capillary electrophoresis (2-3 issues per year)and proteomics. The journal also publishes proceedings of international and national electrophoresis meetings.

  • Impact factor
    3.26
  • 5-year impact
    2.87
  • Cited half-life
    7.10
  • Immediacy index
    0.48
  • Eigenfactor
    0.03
  • Article influence
    0.63
  • Website
    Electrophoresis website
  • Other titles
    Electrophoresis (Online), Electrophoresis, Proteomics reviews
  • ISSN
    1522-2683
  • OCLC
    43388561
  • Material type
    Periodical, Program, Internet resource
  • Document type
    Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

John Wiley & Sons

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • See Wiley-Blackwell entry for articles after February 2007
    • On personal web site or secure external website at authors institution
    • Not allowed on institutional repository
    • JASIST authors may deposit in an institutional repository
    • Non-commercial
    • Pre-print must be accompanied with set phrase (see individual journal copyright transfer agreements)
    • Published source must be acknowledged with set phrase (see individual journal copyright transfer agreements)
    • Publisher's version/PDF cannot be used
    • Articles in some journals can be made Open Access on payment of additional charge
    • 'John Wiley and Sons' is an imprint of 'Wiley-Blackwell'
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context capillary electrophoresis (CE) is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses - using special detection techniques, using sample preconcentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation. Although it can be performed in different ways depending on where the reaction takes place, this article reviews one of the simplest and at the same time most useful approaches, on-capillary derivatisation. Even if in many cases the use of on-capillary derivatisation alone is enough to improve the detection sensitivity, on other occasions it needs to be employed in combination with the other above-mentioned strategies. After a simple discussion of derivatisation in general, special attention is focused on the on-capillary approach and the methodologies available for on-capillary reactant mixing. Its applications in various fields are also described. This article is protected by copyright. All rights reserved.
    Electrophoresis 10/2014;
  • Electrophoresis 10/2014; 35(19).
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this paper, we present a MOSFET-based (metal oxide semiconductor field-effect transistor) microfluidic gate to characterize the translocation of red blood cells (RBCs) through a gate. In the microfluidic system, the bias voltage modulated by the particles or biological cells is connected to the gate of MOSFET. The particles or cells can be detected by monitoring the MOSFET drain current instead of DC/AC-gating method across the electronic gate. Polystyrene particles with various standard sizes are utilized to calibrate the proposed device. Furthermore, RBCs from both adults and newborn blood sample are used to characterize the performance of the device in distinguishing the two types of RBCs. As compared to conventional DC/AC current modulation method, the proposed device demonstrates a higher sensitivity and is capable of being a promising platform for bio-assay analysis.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: One of the main factors that can affect the quality of microarray results is the microarray hybridization specificity. The key factor that affects hybridization specificity is the design of the probes. In this paper, we described a novel oligonucleotide probe containing deoxyinosines aimed at improving DNA hybridization specificity. We compared different probes to determine the distance between deoxyinosine base and SNPs site and the number of deoxyinosine bases. The new probe sequences contained two set of deoxyinosines (each set had two deoxyinosines), in which the interval between SNP site and each set of deoxyinosines was two bases. The new probes could obtain the highest hybridization specificity. The experimental results showed that probes containing deoxyinosines hybridized effectively to the perfectly matched target and improved the hybridization specificity of DNA microarray. By including a simple washing step after hybridization, these probes could distinguish matched targets from single-base-mismatched sequences perfectly. For the probes containing deoxyinosines, the fluorescence intensity of a match sequence was more than 8 times stronger than that of a mismatch. However, the intensity ratio was only 1.3 times or less for the probes without deoxyinosines. Finally, using hybridization of the polymerase chain reaction (PCR) product microarrays, we successfully genotyped single-nucleotide polymorphism (SNP) of 140 samples using these new labeled probes. Our results show that this is a useful new strategy for modifying oligonucleotide probes for use in DNA microarray analysis.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The advance of glycoproteomic technologies has offered unique insights into the importance of glycosylation in determining the functional roles of a protein within a cell. Biologically active glycoproteins include the categories of enzymes, hormones, proteins involved in cell proliferation, cell membrane proteins involved in cell-cell recognition and communication events or secreted proteins, just to name a few. The recent progress in analytical instrumentation, methodologies and computational approaches has enabled a detailed exploration of glycan structure, connectivity and heterogeneity, underscoring the staggering complexity of the glycome repertoire in a cell. A variety of approaches involving the use of spectroscopy, mass spectrometry, separation, microfluidic and microarray technologies have been used alone or in combination to tackle the glycoproteome challenge, the research results of these efforts being captured in an overwhelming number of annual publications. This work is aimed at reviewing the major developments and accomplishments in the field of glycoproteomics, with focus on the most recent advancements (2012–2014) that involve the use of capillary separations and mass spectrometry detection.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel catanionic surfactants vesicle system composed of octyltriethylammonium bromide/ sodium dodecyl benzene sulfonate (C8NE3Br/SDBS) has been developed as pseudostationary phase (PSP) in electrokinetic chromatography (EKC). The C8NE3Br/SDBS system possesses a large vesicle phase region and none of agglomeration phenomena appeared while mixing cationic and anionic surfactants at any molar ratio. Electrophoretic and chromatographic parameters including elution window, hydrophobic selectivity, polar group selectivity and shape selectivity were characterized using the vesicle at molar ratio of C8NE3Br to SDBS of 3:7 as PSP. Compared with sodium dodecyl sulfate (SDS) micelles, the vesicle PSP possessed a wider elution window and a better selectivity. The retention behavior and selectivity differences between the novel vesicle and SDS micelles were evaluated through linear solvation energy relationship (LSER) analysis. Though the cohesiveness and the hydrogen bond acidity have greatest influences on the solutes retention and selectivity in both the vesicle and SDS micelle, the vesicle PSP demonstrated a higher hydrophobicity and a lower hydrogen bonding donating capability owing to compact bilayer structure of vesicle. Additionally, the vesicle system had a stronger hydrogen bond accepting capability than SDS micelle. Consequently, according to LSER analysis, the bigger coefficients for v, b, and a revealed the vesicle PSP had a better separation selectivity than conventional SDS micelle.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Bare gold nanoparticles selectively enhance the Raman signal of beta-agnonists in swine hair extract at 780 nm, which enables analysis of beta-agonists in swine hair extract without chemical labeling, purification or separation. The analysis is multiplexable and the LOD of beta-agonists is around ng/ml in the assistance of microfluidic paper.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The interpretation of phosphoproteomics data sets is crucial for generating hypotheses that guide therapeutic solutions, yet not many techniques have been applied to this type of analysis. This paper intends to give an overview about the two main standard techniques that can be applied to the analysis of these large scale data sets. These are data-driven or exploratory techniques based on a statistical model and topology-driven methods that analyze the signaling network from a dynamical standpoint. While employing different paradigms, these algorithms will detect unique “fingerprints” by revealing the intricate interactions at the proteome level and will support the experimental environment for novel therapeutics for many diseases.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The capacitance-to-digital single chip detector was upgraded. The paper discusses hardware issues and benefits of the designed/ upgraded detector. The device can be operated from rechargeable lithium-ion battery as stand-alone, portable system and is capable of transmitting real-time data wirelessly. The detector and additional modules (battery, battery holder, microcontroller board, wireless module) weight is less than 85 g. Electrophoretic separation in low conductivity 20 mm MES/ L-His buffer, pH 6.1, was performed in order to evaluate detection parameters. The system is capable of quantification of potassium ions down to 0.31 μM. Investigation of differential signal acquisition configuration showed improved performance regarding external noise and temperature fluctuations. The system can be a solution for stand-alone, field-portable capillary format separation detector.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The detection of oligoclonal bands (OCBs) in cerebrospinal fluid is an indicator of intrathecal synthesis of immunoglobulins which is a neurochemical sign of chronic inflammatory brain diseases. Intrathecally synthesized IgGs are typically observed in patients with multiple sclerosis. The current standard protocol for the detection of OCBs is isoelectric focusing on agarose or polyacrylamide gels followed by immunoblotting or silver staining. These methods are time consuming, show substantial inter-laboratory variation and cannot be used in a high throughput-approach. We have developed a new nanoscale method for the detection of OCBs based on automated capillary isoelectric focusing followed by immunological detection. Evidence for intrathecal IgG synthesis was found in all tested patients (n = 27) with multiple sclerosis, even in two subjects who did not have oligoclonal bands according to standard methods. The test specificity was at 97.5% (n = 19). Our findings indicate that the novel OCB-CIEF-immunoassay is suitable for the rapid and highly sensitive detection of OCBs in clinical samples. Furthermore, the method allows for a higher sample throughput than the current standard methods.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol) diacrylate) cryogel placed in the centrifugal filter device. The composition of the polymerization mixture as well as the polymerization conditions were optimized in order to prepare a material with bimodal pore size distribution with 20–50 μm flow throw macropores and submicrometer pores in the polymer walls. The optimized, mechanically stable, highly porous, material was used for spin column lectin chromatography. The surface of the monolithic scaffold was activated by epichlorohydrin and used for immobilization of concanavalin A to provide the affinity supports for selective isolation of glycoproteins containing high mannose glycan structures. The performance of the developed lectin modified cryogels was evaluated by analyses of glycoprotein mixtures. The efficiency and selectivity of the affinity supports were confirmed by MALDI-MS analysis.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this work, an electrophoretically mediated microanalysis (EMMA) method with a partial filling technique was setup to evaluate the inhibitory potency of novel compounds toward aminopeptidase N (APN). It was necessary to optimize the electrophoretic conditions with respect to the kinetic constraints and for attaining high sensitivity. In our set-up a part of the capillary was filled with the incubation buffer for the enzyme reaction, whereas the rest was filled with a suitable background electrolyte for the separation of substrates and products. To monitor the performance of the newly developed method, the kinetic constants (Km and Vmax) for the catalyzed dissociation of L-Leucine-p-nitroanilide (Leu-pNA) in the presence of APN as well as the inhibition constant (IC50) of a known competitive inhibitor, i.e. bestatin, were determined and these results were compared with those obtained by a classical spectrophotometric assay. The developed EMMA method was subsequently applied to the screening of 30 APN inhibitors. Whereas the inhibition potency of these inhibitors (expressed in IC50 values) were significantly underestimated by the EMMA method, the order of the inhibitory potential of these various compounds was found in agreement with the literature.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Counting of E. coli DH5α cell suspensions in phosphate buffered saline is performed using a micro-flow cytometer based on a photonic-microfluidic integrated device. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a haemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2–4μm) as their scattering intensities are different.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: This review article expands on the previous one (S. Selvaraju and Z. El Rassi, Electrophoresis 2012, 33, 74–88) by reviewing pertinent literature in the period extending from early 2011 to present. As the previous review article, the present one is concerned with proteomic sample preparation (e.g., depletion of high abundance proteins, reduction of the protein dynamic concentration range, enrichment of a particular sub-proteome), and the subsequent chromatographic and/or electrophoretic pre-fractionation prior to peptide separation and identification by LC-MS/MS. This review article is distinguished from its second version published in Electrophoresis 2012, 33, 74–88 by expanding on capturing/enriching sub-phosphoproteomes by immobilized metal affinity chromatography and metal oxide affinity chromatography. Seventy-seven papers published in the period extending from mid 2011 to the present have been reviewed. By no means this review article is exhaustive, given the fact that its aim is to give a concise treatment of the latest developments in the field.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The combination of capillary electrophoresis (CE) and mass spectrometry (MS) is now a widely used tool that can provide a combination of high resolution separations with detailed structural information. Recently we highlighted the benefits of an approach to add further functionality to this well-established hyphenated technique, namely the possibility to perform chemical reactions within the sheath- liquid of the CE-MS interface [1]. Apart from using hydrogen/deuterium exchange for on-line determination of numbers of exchangeable protons, the addition of DPPH• (2,2-diphenyl-1-picrylhydrazyl) to the sheath- liquid can be used as a fast screening tool for studying antioxidant characteristics of individual components. Such a CE-MS methodology allows rapid and information-rich analysis with minimal reagent and sample consumption to be performed. In the present work, we demonstrate the applicability of this approach for the characterization of phenolic plant extracts from the Labiatae family, namely Rosmarinus officinalis and Melissa officinalis. Using the described approach, a wide range of compounds (15 and 13 phenolic compounds respectively) could be confidently identified using a combination of high resolution CE-MS separations with implementation of online deuterium exchange and DPPH• reactions. These compounds included polyphenols, phenolic acids and triterpene acids. In conjunction with online MS/MS experiments, extensive structural information for aglyconic and glycosylated antioxidants present in the extracts could be obtained using simple experimental changes, which can be carried out prior to the purchasing of expensive chemical standards or the time-consuming preparative isolation of individual compounds.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Counter-flow gradient electrofocusing allows the simultaneous concentration and separation of analytes by generating a gradient in the total velocity of each analyte which is the sum of its electrophoretic velocity and the bulk counter-flow velocity. In the scanning format, the bulk counter-flow velocity is varying with time so that a number of analytes with large differences in electrophoretic mobility can be sequentially focused and passed by a single detection point. Studies have shown that nonlinear (such as a bilinear) velocity gradients along the separation channel can improve both peak capacity and separation resolution simultaneously, which cannot be realized by using a single linear gradient. Developing an effective separation system based on the scanning counter-flow nonlinear gradient electrofocusing technique usually requires extensive experimental and numerical efforts, which can be reduced significantly with the help of analytical models for design optimization and guiding experimental studies. Therefore, this study focuses on developing an analytical model to evaluate the separation performance of scanning counter-flow bilinear gradient electrofocusing methods. In particular, this model allows a bilinear gradient and a scanning rate to be optimized for the desired separation performance. The results based on this model indicate that any bilinear gradient provides a higher separation resolution (up to 100%) compared to the linear case. This model is validated by numerical studies.This article is protected by copyright. All rights reserved
    Electrophoresis 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Herein, we present a straightforward surface modification technique for PDMS-based microfluidic devices. The method takes advantage of the high reactivity of concentrated sulfuric acid to enhance the surface properties of PDMS bulk material. This results in alteration of the surface morphology and chemical composition which is in-depth characterized by ATR-FTIR, EDX, SEM and XPS. In comparison to untreated PDMS, modified substrates exhibit a significantly reduced diffusive uptake of small organic molecules while retaining its low electroosmotic properties. This was demonstrated by exposing the channels of a microfluidic device to concentrated rhodamine B solution followed by fluorescence microscopy. The surface modification procedure was used to improve chip-based electrophoretic separations. Separation efficiencies of fluorescein isothiocyanate labelled amines / amino acids obtained in treated and untreated PDMS-devices as well as in glass chips were compared. We obtained higher efficiencies in H2SO4 treated PDMS chips compared to untreated ones but lower efficiencies than those obtained in commercial microfluidic glass devices This article is protected by copyright. All rights reserved.
    Electrophoresis 09/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The high frequency dielectrophoresis (>20 MHz) response of microalgae cells with different lipid content was monitored over time. Chlamydomonas reinhardtii was cultured in regular medium and under nitrogen-depleted conditions in order to produce populations of cells with low and high lipid content, respectively. The electrical conductivity (EC) of the culture media was also monitored over the same time. The upper crossover frequency (UCOF) decreased for high-lipid cells over time. The single-shell model predicts that the upper crossover frequency is dictated primarily by the dielectric properties of the cytoplasm. The high frequency DEP response of the high-lipid cells' cytoplasm was changed by lipid accumulation. DEP response of the low-lipid cells also varied with the conductivity of the culture media due to nutrient consumption. Relative lipid content was estimated with BODIPY 505/515 dye by calculating the area-weighted intensity average of fluorescent images. Finally, microalgae cells were successfully separated based on lipid content at 41 MHz and DEP media conductivity 106 ± 1 μS/cm. This article is protected by copyright. All rights reserved.
    Electrophoresis 09/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A set of 33 drugs with different solubility, ranging to soluble to very insoluble ones, has been chosen in order to evaluate the performance of the internal standard capillary electrophoresis method (IS-CE) to determine acidity constants of compounds with limited solubility. The set tested in this work has been chosen in function of their intrinsic solubility. For the most insoluble compounds several analytical conditions to overcome the insolubility in aqueous buffers have been tested. This paper assesses the compound solubility limits for the IS-CE method in aqueous pKa determinations, as well as compares the determined pKa s with the results from literature data obtained by other methods. It is proved that IS-CE method is able to determine acidity constants of sparingly soluble drugs in aqueous media (compounds with logs down to around -6) whereas other reference methods require the use of water-organic solvent buffers and extrapolation procedures to obtain the aqueous pKa for the same compounds. This article is protected by copyright. All rights reserved.
    Electrophoresis 09/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new fluorescent prestaining method for gel-separated glycoproteins in 1-D and 2-D SDS-PAGE was developed by using 4H-[1]-Benzopyrano[4,3-b]thiophene-2-carboxylic acid hydrazide (BH). The prestained gels were readily imaged after electrophoresis without any time-consuming steps needed for poststain. As low as 4-8 ng glycoproteins (transferrin, α1-acid glycoprotein) could be selectively detected, which is comparable to the most commonly used Pro-Q Emerald 488 glycoprotein stain. In addition, subsequent study of deglycosylation, glycoprotein affinity chromatography, and LC-MS/MS analysis were performed to confirm the specificity of the newly developed method. As a result, BH prestain provides a new choice for quick, sensitive, specific, economical and MS compatible visualization of gel-separated glycoproteins. This article is protected by copyright. All rights reserved.
    Electrophoresis 09/2014;