Biological Procedures Online Journal Impact Factor & Information

Publisher: BioMed Central

Journal description

Biological Procedures Online publicizes new research techniques or novel adaptations of existing techniques and permits authors to supplement their previously published research with additional procedural information...BPO is a free service. Articles may be accessed without charge or registration and can be freely printed to paper for non-profit or educational use. Authors retain copyright ownership of their work. These liberal copyright policies are among the most progressive in scientific publishing.

Current impact factor: 1.79

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 1.792
2013 Impact Factor 1.3
2012 Impact Factor 0.95
2011 Impact Factor 1.29
2010 Impact Factor 0.742
2009 Impact Factor 0.75
2008 Impact Factor 2.273
2007 Impact Factor 1.179

Impact factor over time

Impact factor

Additional details

5-year impact 2.70
Cited half-life 8.20
Immediacy index 0.40
Eigenfactor 0.00
Article influence 0.89
Website Biological Procedures Online (BPO) website
Other titles Biological procedures online, BPO
ISSN 1480-9222
OCLC 43835506
Material type Document, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

BioMed Central

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Publisher's version/PDF may be used
    • Eligible UK authors may deposit in OpenDepot
    • Creative Commons Attribution License
    • Copy of License must accompany any deposit.
    • All titles are open access journals
    • 'BioMed Central' is an imprint of 'Springer Verlag (Germany)'
  • Classification

Publications in this journal

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    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Target selection for oncology is a crucial step in the successful development of therapeutics. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 editing of specific loci offers an alternative method to RNA interference and small molecule inhibitors for determining whether a cell line is dependent on a specific gene product for proliferation or survival. In our initial studies using CRISPR-Cas9 to verify the dependence on EZH2 activity for proliferation of a SMARCB1/SNF5/INI1 mutant malignant rhabdoid tumor (MRT) cell line, we noted that the initial reduction in proliferation was lost over time. We hypothesized that in the few cells that retain proliferative capacity, at least one allele of EZH2 remains functional. To verify this, we developed an assay to analyze 10s-100s of clonal cell populations for target gene disruption using restriction digest and fluorescent fragment length analyses. Results: Our results clearly show that in cell lines in which EZH2 is essential for proliferation, at least one potentially functional allele of EZH2 is retained in the clones that survive. Conclusion: This assay clearly indicates whether or not a specific gene is essential for survival and/or proliferation in a given cell line. Such data can aid the development of more robust therapeutics by increasing confidence in target selection.
    Biological Procedures Online 12/2015; 17(1). DOI:10.1186/s12575-015-0028-4
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    ABSTRACT: Many studies have correlated characteristics of amino acids with crystallization propensity, as part of the effort to determine the factors that affect the propensity of protein crystallization. However, these characteristics are constant; that is, the encoded amino acid sequences have the same value for each type of amino acid. To overcome this inflexibility, three dynamic characteristics of amino acids and protein were introduced to analyze the crystallization propensity of proteins. Both logistic regression and neural network models were used to correlate each of two dynamic characteristics with the crystallization propensity of 301 proteins from Arabidopsis thaliana, and their results were compared with those obtained from each of 531 constant amino acid characteristics, which served as the benchmark. The neural network model was more powerful for predicting the crystallization propensity of proteins than the logistic regression model. Compared with the benchmark, the dynamic characteristics of amino acids provided good prediction results for the crystallization propensity, and the distribution probability gave the highest sensitivity. Using 90 % accuracy as a cutoff point, the predictable portion of A. thaliana portions was ranked, and the statistical analysis showed that the larger the predictable portion, the better the prediction. These results demonstrate that dynamic characteristics have a certain relationship with the crystallization propensity, and they could be helpful for the prediction of protein crystallization, which may provide a theoretical concept for certain proteins before conducting experimental crystallization.
    Biological Procedures Online 12/2015; 17(1). DOI:10.1186/s12575-015-0029-3
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    ABSTRACT: Super resolution (SR) microscopy enabled cell biologists to visualize subcellular details up to 20 nm in resolution. This breakthrough in spatial resolution made image analysis a challenging procedure. Direct and automated segmentation of SR images remains largely unsolved, especially when it comes to providing meaningful biological interpretations. Here, we introduce a novel automated imaging analysis routine, based on Gaussian, followed by a segmentation procedure using CellProfiler software ( We tested this method and succeeded to segment individual nuclear pore complexes stained with gp210 and pan-FG proteins and captured by two-color STED microscopy. Test results confirmed accuracy and robustness of the method even in noisy STED images of gp210. Our pipeline and novel segmentation procedure may benefit end-users of SR microscopy to analyze their images and extract biologically significant quantitative data about them in user-friendly and fully-automated settings.
    Biological Procedures Online 12/2015; 17(1):11. DOI:10.1186/s12575-015-0023-9
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    ABSTRACT: Contributing reviewers A peer-reviewed journal would not survive without the generous time and insightful comments of the reviewers, whose efforts often go unrecognized. Although final decisions are always editorial, they are greatly facilitated by the deeper technical knowledge, scientific insights, understanding of social consequences, and passion that reviewers bring to our deliberations. For these reasons, the Editor-in-Chief and staff of the journal warmly thank the reviewers whose comments helped to shape Biological Procedures Online, for their invaluable assistance with review of manuscripts for the journal in Volume 16 (2014).
    Biological Procedures Online 12/2015; 17(1):5. DOI:10.1186/s12575-015-0017-7
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    ABSTRACT: Background: Thermal denaturation of probe-target hybrid is highly reproducible, and which makes probe melting point analysis reliable in the detection of mutations, polymorphisms and epigenetic differences in DNA. To improve resolution of these detections, we used dual-labeled (quencher and fluorescence), full base of peptide nucleic acid (PNA) probe for fluorescence probe based melting point analysis. Because of their uncharged nature and peptide bond-linked backbone, PNA probes have more favorable hybridization properties, which make a large difference in the melting temperature between specific hybridization and partial hybridization. Results: Here, we have shown that full base dual-labeled PNA is apt material for fluorescence probe-based melting point analysis with large difference in the melting temperature between full specific hybridization and that of partial hybridization, including insertion and deletion. In case of narrowly distributed mutations, PNA probe effectively detects three mutations in a single reaction tube with three probes. Moreover, we successfully diagnose virus analogues with amplification and melting temperature signal. Lastly, Melting temperature of PNA oligomer can be easily adjusted just by adding gamma-modified PNA probe. Conclusions: The PNA probes offer advantage of improved flexibility in probe design, which could be used in various applications in mutation detection among a wide range of spectrums.
    Biological Procedures Online 11/2015; 17(1):14. DOI:10.1186/s12575-015-0027-5
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    ABSTRACT: Alternative methods are being sought to measure the potency of influenza vaccines. Label-free technologies that do not require the use of hemagglutinin (HA)-specific antisera are particularly attractive as the preparation of antiserum delays availability of potency reagents. The objective of these experiments was to evaluate the use of a Corning EpicĀ® label-free method to quantify functional influenza hemagglutinin in rHA preparations. The method was optimized to quantify recombinant HA (rHA) of B/Brisbane/60/2008 (B/BR/08). Fetuin was immobilized onto plates and the change in wavelength of refracted light measured using an Enspire (Perkin Elmer) instrument. The change in wavelength measured in response to addition of rHA of B/BR/08 was proportional to its concentration and was optimal in the presence of native rHA conformations. However, the assay was strain-dependent and did not correlate with HAU measured using turkey red blood cells. The Corning EpicĀ® label-free method is suitable for quantifying the native forms of rHA for B/BR/08 and A/Brisbane/59/2007 (H1N1) and A/Hangxhou/3/2013 (H7N9). This method is a useful tool for research purposes but further investigation is needed to identify suitable glycoproteins to use as ligands that allow quantification of HAs from a broader range of virus strains.
    Biological Procedures Online 03/2015; 17(1):7. DOI:10.1186/s12575-015-0019-5
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    ABSTRACT: We have previously established technologies enabling us to engineer the Mycoplasma mycoides genome while cloned in the yeast Saccharomyces cerevisiae, followed by genome transplantation into Mycoplasma capricolum recipient cells to produce M. mycoides with an altered genome. To expand the toolbox for genomic modifications, we designed a strategy based on the Cre/loxP-based Recombinase-Mediated Cassette Exchange (RMCE) system for functional genomics analyses. In this paper, we demonstrated replacement of an approximately 100 kb DNA segment of the M. mycoides genome with a synthetic DNA counterpart in two orientations. The function of the altered genomes was then validated by genome transplantation and phenotypic characterization of the transplanted cells. This method offers an easy and efficient way to manipulate the M. mycoides genome and will be a valuable tool for functional genomic studies, such as genome organization and minimization.
    Biological Procedures Online 03/2015; 17(1):6. DOI:10.1186/s12575-015-0016-8
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    ABSTRACT: Long non-coding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides. The post-transcriptional regulation is influenced by these lncRNAs by interfering with the microRNA pathways, involving in diverse cellular processes. The regulation of gene expression by lncRNAs at the epigenetic level, transcriptional and post-transcriptional level have been well known and widely studied. Recent recognition that lncRNAs make effects in many biological and pathological processes such as stem cell pluripotency, neurogenesis, oncogenesis and etc. This review will focus on the functional roles of lncRNAs in epigenetics and related research progress will be summarized.
    Biological Procedures Online 09/2014; 16(1):11. DOI:10.1186/1480-9222-16-11