Planta Medica

Publisher: Gesellschaft für Arzneipflanzenforschung, Thieme Publishing

Description

  • Impact factor
    2.35
  • 5-year impact
    2.46
  • Cited half-life
    0.00
  • Immediacy index
    0.30
  • Eigenfactor
    0.01
  • Article influence
    0.53
  • Other titles
    Planta medica (En ligne), Planta medica
  • ISSN
    1439-0221
  • OCLC
    182630769
  • Material type
    Document, Periodical, Internet resource
  • Document type
    Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Thieme Publishing

  • Pre-print
    • Author cannot archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • On author's personal website immediately
    • On Institutional Repository and PubMed Central after 12 months embargo
    • Publisher's version/PDF can be used on author's personal website only
    • Publisher copyright and source must be acknowledged
    • Link to Publisher version (www.thieme-connect.com) must be included if article has been published online
  • Classification
    ​ blue

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Two lanostane triterpenes, 3β-hydroxylanosta-9,24-dien-21-oic acid (1) and methyl-3β-hydroxylanosta-9,24-dien-21-oate (2), were isolated from the stem bark of Protorhus longifolia. Their structures were deduced on the basis of spectroscopic analysis (NMR, HRMS, IR). This study investigated the in vitro anti-adipogenic activity of the two triterpenes. Their inhibitory activity was evaluated on selected lipid digestive enzymes (pancreatic lipase and cholesterol esterase). The inhibitory activity of the compounds on hormone-sensitive lipase and their ability to bind bile acids were also evaluated. The effect of the compounds on glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes, and on triglyceride accumulation in 3T3-L1 adipocytes was investigated. The triterpenes effectively inhibited the activities of the enzymes with IC50 values ranging from 0.04 to 0.31 mg/mL. The compounds showed a high affinity for secondary bile acids. Both compounds stimulated glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes. Compound 1 significantly reduced triglyceride accumulation in mature differentiated 3T3-L1 adipocytes. It is apparent that these lanostane triterpenes enhance glucose uptake and suppress adipogenesis, which together with their inhibitory effects on lipid digestive enzymes suggests that they have antihyperlipidemic potential.
    Planta Medica 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hypericum perforatum (HP), an important medicinal plant that possesses several pharmacological properties, remains recalcitrant to Agrobacterium tumefaciens (AT) mediated transformation. Upon co-cultivation, AT viability was reduced completely HP cells defense response [1]. Further, we reported that the antibacterial and antioxidant activities of HP cells were increased several fold after elicitation with AT that provide antimicrobial and antioxidant protection to HP cells [2]. Recently, our group has demonstrated that the AT- elicited HP extract render protection against oxidative stress induced in human HepG2 cells [3]. Here, we report that the antibacterial activity of methanolic extract of HP cells elicited with AT against important human bacterial pathogens (Staphylococcus aureus and Bacillus subtilis). Briefly, equivalent concentration of methanolic extract of elicited and control HP cells were added to overnight cultures of S. aureus and B. subtilis. After 24h of incubation, the bacterial cultures were serially diluted and spread on LB plates. Colony forming units were counted after 2 – 3 days of incubation of plates at 37 ° C. Among various dilutions checked, 10 – 6 dilution exhibited countable number of colonies in cultures without any treatment (control) and un-elicited HP extract treatment. Whereas, countable number of colonies in elicited extract treatments was only found in 10 – 1 dilution revealing that the antibacterial activity of elicited HP cell extract against these pathogens is 105 times higher than un-elicited extract. Our results reveal that the increase in antimicrobial activity in AT elicited HP cells is not restricted to AT, a phytopathogen, but also extends to human pathogens. Testing of several other human bacterial pathogens, resistant to antibiotics is ongoing in our lab.
    Planta Medica 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hypericum perforatum (HP), a high value medicinal plant used to produce several phytopharmaceuticals, remains recalcitrant to Agrobacterium tumefaciens (AT) mediated transformation. HP recognizes AT as a potential pathogen and activates its defense response [1]. Although elicitation of HP cells with AT is known to increase its xanthone content, flavonoids remained unaffected in the soluble fraction of HP [2]. However, specific analysis of HP cell wall biomass/fractions after AT elicitation revealed an increase in lignin and cell wall bound flavonoids content, possibly as mechanism of defense against AT. Briefly, cell wall biomass was isolated from control and AT elicited HP cells via consecutive extraction with hexane, acetone, methanol and distilled water. Determination of lignin content by acetyl bromide method [3] showed a significant increase in HP cells treated with AT, implying that upon elicitation by AT, HP reinforced its cell wall as a defense response. In order to analyze their phenolic profile, the cell wall biomass was subjected to extraction by 90% methanol after alkaline hydrolysis, HCl titration and ethyl acetate extraction. HPLC analysis of the cell wall bound phenolics fraction revealed an accumulation of flavonoids (e.g. epicatechin, quercetin derivatives) in the cell walls after elicitation. Upregulation of chalcone synthase (CHS), leucoanthocyanidin dioxygenase (LDOX) and Flavone 3-hydroxilase (F3 H) were found to be responsible for this accumulation. Further, DPPH reduction and TBARS assays clearly correlated the increased production of flavonoids with improved antioxidant protection of the cell wall. Collectively, our results allow us to conclude that in addition to the increase in xanthone production [2], co-cultivation of HP cells with AT also upregulates flavonoids biosynthesis as a defense mechanism. While the xanthones enrich the soluble phenolic fraction; flavonoids are incorporated into the cell wall.
    Planta Medica 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Withania sominifera (L) is a well-known Indian medicinal plant used to treat stress, arthritis insomnia and age related disorders including neurodegenerative disorders. It has several vernacular names including aswaganda and ginseng winter cherry [1 – 2]. Withanolides are the major and characteristic active compounds of this species [3]. The isolated fraction of withanolides have been studied for their anti-tumor, antiinflammatory, anti-stress, anti-oxidant, immuno-modulatory, cardio-protective and neuroprotective activity [4]. To enhance the solubility, bioavailability, protection from toxicity and increase the pharmacological activity of herbal extracts, several approaches has been proposed. Among them, plant extract based novel drug delivery system attracted much attention. In this investigation, we have developed and characterized a purified W. somnifera extract (WSE) encapsulated in PCL and MPEG-PCL nanoparticles and identified the entrapped compounds by reverse phase high performance liquid chromatography (RP-HPLC). WSE was obtained from leaf biomass extraction with aqueous MeOH and further purification with dichloromethane. MPEG-PCL di-block polymer was synthesised by ring opening polymerization of ε-Caprolactone on MPEG using Sn(Oct)2 as catalyst. Prepared WSE-nanoparticles were characterised by laser doppler anemometry and Transmission electron microscopy (TEM). The results from TEM and laser doppler anemometry confirmed that the size of PCL-WSE and MPEG-PCL-WSE nanoparticles ranged between 220 – 250nm and spherical in shape (Figure 1). The MPEG-PCL-WSE nanoformulation showed higher entrapment efficacy (54.4%) than PCL-WSE nanoparticles (31.2%). The RP-HPLC analysis revealed that Withanolide A, Withaferin-A, Withanolide-B were major compounds encapsulated in the nanoparticles.
    Planta Medica 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Curcumin is a natural polyphenolic compound isolated from turmeric (Curcuma longa), with well-demonstrated neuroprotective and anticancer activities [1]. Although curcumin is safe even at high doses in humans, it exhibits poor bioavailability mainly due to poor absorption, fast metabolism, and rapid systemic elimination. To overcome these hurdles, several approaches such as nanoparticle mediated targeted delivery have been undertaken with different degrees of success [2 – 4]. The present study was conducted to identify the neuroprotective effect of curcumin encapsulated into polymeric PCL and MPEG-PCL nanoparticles in U251 glioblastoma cells. MPEG-PCL di-block polymer was synthesised by ring opening polymerization of ε-Caprolactone on MPEG using Sn(Oct)2 as catalyst. Both PCL and MPEG-PCL nanoparticles were prepared by solvent displacement technique and were physically characterised by laser doppler anemometry, transmission electron microscopy (TEM) and X-Ray diffraction (XRD). The results from laser doppler anemometry confirmed that size of PCL and MPEG-PCL nanoparticles ranged between 200 – 240nm and TEM images revealed the spherical shape of nanoparticles (Figure 1A). Results of the cytotoxicity assay showed that the empty and curcumin encapsulated nanoparticles (PCL, MPEG-PCL) were nontoxic to U251 cells (up to 500 µg/ml). Moreover, MPEG-PCL loaded curcumin nanoparticles were able to protect cells against tBHP induced oxidative damage in a superior way (up to 61%) compared to free curcumin 1 µg/ml. This was in part due a higher absorption of the cells by curcumin-MPEG-PCL nanoparticles (Figure 1C). Together, our results show that curcumin-MPEG-PCL nanoparticles possess significantly higher neuroprotective effect in cells compared to free curcumin and curcumin-PCL nanoparticles.
    Planta Medica 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Withania somnifera (L) Dunal, (Solanaceae), commonly known as ashwagandha, winter cherry or Indian ginseng, is one of the distinguished medicinal plants in Indian Ayurvedic medicine [1]. Although W. somnifera was known for its medicinal uses since ancient times, it received much more attention in the recent years from pharmaceutical companies and local Baidyas due to confirmation of its multipurpose medicinal uses [2]. Several pharmacological studies have demonstrated that W. somnifera is efficacious in the treatment of arthritis, geriatric, behavioural and stress related problems [3]. In the present study, we have biosynthesised silver nanoparticles (AgNPs) using W. somnifera aqueous leaf extract, incorporated them into a cream formulation and evaluated the anti-bacterial potential of the cream against several pathogenic bacteria (Stapylococous aureus, Pseudomonas aruginosa, Candida albicans, Proteus vulgarius, Escherichia coli, Agrobacterium tumefaciens). The formation, size and shape of biosynthesized AgNPs were confirmed by physical-chemical techniques such as UV-Visible spectroscopy, Laser Doppler anemometry, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), X-ray diffraction (XRD) and X-ray energy dispersive spectroscopy (EDX). AgNPs exhibited significantly higher antibacterial activity (up to 200x) against human as well as plant pathogens compared to AgNO3 solution or W. somnifera leaf extract. The cellular interaction study coupled with SEM analysis revealed the effective disruption of bacterial cell by AgNPs. The incorporation of AgNO3 and AgNPs into a cream was done. The AgNPs-cream did not provoque significant changes in physical properties and stability of the cream, when compared with control, but have a significant higher antibacterial activity (Figure 1).
    Planta Medica 10/2014; 80.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hypericum perforatum (HP), a high value medicinal plant used to produce several phytopharmaceuticals, remains recalcitrant to Agrobacterium tumefaciens (AT) mediated transformation. HP recognizes AT as a potential pathogen and activates its defense response [1]. Although elicitation of HP cells with AT is known to increase its xanthone content, flavonoids remained unaffected in the soluble fraction of HP [2]. However, specific analysis of HP cell wall biomass/fractions after AT elicitation revealed an increase in lignin and cell wall bound flavonoids content, possibly as mechanism of defense against AT. Briefly, cell wall biomass was isolated from control and AT elicited HP cells via consecutive extraction with hexane, acetone, methanol and distilled water. Determination of lignin content by acetyl bromide method [3] showed a significant increase in HP cells treated with AT, implying that upon elicitation by AT, HP reinforced its cell wall as a defense response. In order to analyze their phenolic profile, the cell wall biomass was subjected to extraction by 90% methanol after alkaline hydrolysis, HCl titration and ethyl acetate extraction. HPLC analysis of the cell wall bound phenolics fraction revealed an accumulation of flavonoids (e.g. epicatechin, quercetin derivatives) in the cell walls after elicitation. Upregulation of chalcone synthase (CHS), leucoanthocyanidin dioxygenase (LDOX) and Flavone 3-hydroxilase (F3 H) were found to be responsible for this accumulation. Further, DPPH reduction and TBARS assays clearly correlated the increased production of flavonoids with improved antioxidant protection of the cell wall. Collectively, our results allow us to conclude that in addition to the increase in xanthone production [2], co-cultivation of HP cells with AT also upregulates flavonoids biosynthesis as a defense mechanism. While the xanthones enrich the soluble phenolic fraction; flavonoids are incorporated into the cell wall.
    Planta Medica 10/2014;
  • Planta Medica 10/2014; 80(16).
  • Planta Medica 08/2014; 80:PD138.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Bioassay guided fractionation of the ethanolic extract of Asphodelus microcarpus Salzm.et Viv. (Xanthorrhoeaceae or Asphodelaceae) resulted in the isolation of four new compounds identified as asphodosides A-D. The isolated metabolites showed high binding affinity to cannabinoid receptors (subtype CB1 and CB2).
    Planta Medica 07/2014; 80(10):32.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Plant-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites. Ethanol and ethyl acetate extracts of an endophytic fungus isolated from the root of Mammillaria hahniana (old lady cactus); an endangered American cactus; were found incredibly to be cytotoxic. While searching for compounds with cytotoxic activity, a number of naphtoquinons include shikonin and shikonin derivatives were separated from ethyl acetate and ethanol extracts using chromatographic methods.
    Planta Medica 07/2014; 80(10):PD136.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Karlotoxins, a group of dinoflagellate derived polyketides, have attracted much attention as potential therapeutic compounds due to their unique MoA and selective cytotoxic activities against leukemia and lung cancer in the NCI60 panel. The richly oxygenated C (1 – 17) polyol chain of karlotoxin 5 has been synthesized. Incorporated in this long chain are 6 of the 28 stereogenic centers housed in the target compound. The asymmetric pathway that has been developed is based on repeating a three-step chain elongation strategy consisting of (i) simultaneous reductive O-debenzylation and hydrogenation of alkene with Pd/C catalyst, (ii) oxidation of alcohol, and (iii) Julia-Kocienski olefination.
    Planta Medica 07/2014; 80(10):PK1.
  • [Show abstract] [Hide abstract]
    ABSTRACT: In obese adipose tissue, tumor necrosis factor-α secreted from macrophages plays an important role in the adipocyte dysfunctions, including insulin resistance, lipolytic acceleration, and changes of adipokines, which promote the development of obesity-related complications. Phillyrin, an active ingredient found in many medicinal plants and certain functional foods, elicits anti-obesity and anti-inflammatory properties in vivo. The aim of the current study was to investigate the role of phillyrin in preventing tumor necrosis factor α-induced insulin resistance or lipolytic acceleration in 3T3-L1 adipocytes. Our results showed that phillyrin partially restored insulin-stimulated 2-DOG uptake, which was reduced by tumor necrosis factor-α, with concomitant restoration in serine phosphorylation of insulin receptor substrate-1 and insulin-stimulated Glut4 translocation to plasma membrane. Phillyrin also dose-dependently prevented tumor necrosis factor α-stimulated adipocyte lipolysis with preserved downregulation of perilipin. The mitogen-activated protein kinases and I kappaB kinase activation was promoted in tumor necrosis factor α-stimulated adipocytes, but pretreatment with 40 µM phillyrin inhibited the phosphorylation of extracellular signal-regulated kinases1/2, stress-activated protein kinase/Jun N-terminal kinase and I kappaB kinase (p < 0.05). Moreover, phillyrin could inhibit the expressions of interleukin-6 and monocyte chemoattractant protein-1 induced by tumor necrosis factor-α. Using transwell coculture method with 3T3-L1 adipocytes and RAW 264.7 macrophages, the enhanced productions of tumor necrosis factor-α and free fatty acids in the medium were significantly reduced by phillyrin (p < 0.05). These results indicate that phillyrin exerts a beneficial effect on adipocyte dysfunctions induced by tumor necrosis factor-α through suppression of the activation of I kappaB kinase and N-terminal kinase. Phillyrin may have the potential to ameliorate the inflammatory changes and insulin resistance in obese adipose tissue.
    Planta Medica 07/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Chemical investigation of the ethanol extract of the whole plant of Megacodon stylophorus led to the isolation and identification of two new seco-hopane triterpenoids, 2,3-seco-22(29)-hopene-2-carboxyl-3-aldehyde (1) and 2,3-seco-4(23),22(29)-hopene-2-carboxyl-3-aldehyde (2), along with 10 known compounds, 3-12. All the isolates were reported from this plant for the first time. The structures of compounds 1 and 2 were determined by detailed analysis of their spectral data including 1D and 2D NMR. In addition, compound 1 was further analyzed by X-ray crystallography. Compounds 1-3 were evaluated for their in vitro anti-proliferative activities on HeLa, MCF-7, and Hep-G2 tumor cell lines. Compound 2 was active against the three cell lines with IC50 values of 3.6, 7.5, and 13.6 µM, respectively, while compound 1 exhibited cytotoxicity on MCF-7 (IC50 14.0 µM) and HeLa (IC50 18.2 µM) cell lines. Antimicrobial activities of compounds 1-2 (minimum inhibitory concentration values in the range of 3.12-12.50 mg/mL) were also observed.
    Planta Medica 07/2014;
  • Planta Medica 06/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Aminoalcohol-diterpenoid alkaloids have been reported as the cardioactive components in the lateral roots of Aconitum carmichaeli (Fuzi) according to recent studies. Determination of these effective components is of great significance for quality control purposes for Fuzi. Here we report, for the first, the development and validation of a new method to determine the 13 aminoalcohol-diterpenoid alkaloids in Fuzi by using a simple and accurate solid-phase extraction-liquid chromatography-tandem mass spectrometry. The chromatographic analysis was performed on an ODS column with methanol-0.1 % formic acid (80 : 20, v/v) as the mobile phase. The quantification was performed using MS/MS detection in the positive ion mode with multiple reaction monitoring. Linearity was observed within a range of concentrations of 20-2000 ng/mL. For all the analytes, the r value was greater than 0.9990. The limit of detection and the limit of quantitation were less than 0.5 ng/mL and 2.0 ng/mL, respectively. The intraday and interday precisions were less than 5 % and 10 %, respectively. The accuracy was within the range of 90 to 105 %. This method was successfully applied to determine the 13 aminoalcohol-diterpenoid alkaloids in Fuzi from different origins and with different processing methods.
    Planta Medica 06/2014;
  • Planta Medica 06/2014; 80(8/09):609.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Species of Garcinia have been used to combat malaria in traditional African and Asian medicines, including Ayurveda. In the current study, we have identified antiplasmodial benzophenone and xanthone compounds from edible Garcinia species by testing for in vitro inhibitory activity against Plasmodium falciparum. Whole fruits of Garcinia xanthochymus, G. mangostana, G. spicata, and G. livingstonei were extracted and tested for antiplasmodial activity. Garcinia xanthochymus was subjected to bioactivity-guided fractionation to identify active partitions. Purified benzophenones (1-9) and xanthones (10-18) were then screened in the plasmodial lactate dehydrogenase assay and tested for cytotoxicity against mammalian (Vero) cells. The benzophenones guttiferone E (4), isoxanthochymol (5), and guttiferone H (6), isolated from G. xanthochymus, and the xanthones α-mangostin (15), β-mangostin (16), and 3-isomangostin (17), known from G. mangostana, showed antiplasmodial activity with IC50 values in the range of 4.71-11.40 µM. Artemisinin and chloroquine were used as positive controls and exhibited IC50 values in the range of 0.01-0.24 µM. The identification of antiplasmodial benzophenone and xanthone compounds from G. xanthochymus and G. mangostana provides evidence for the antiplasmodial activity of Garcinia species and warrants further investigation of these fruits as dietary sources of chemopreventive compounds.
    Planta Medica 06/2014; 80(8-9):676-81.