Current Microbiology Journal Impact Factor & Information

Publisher: Springer Verlag

Journal description

Current Microbiology offers a means of rapid publication of timely new information dealing with all aspects of microbial cells including prokaryotes and eukaryotes and, where appropriate, viruses. The topics included are general, medical, and applied microbiology and virology and span the disciplines of physiology, biochemistry, genetics, biotechnology, morphology, taxonomy, diagnostic methods, and immunology as applied to microorganisms. Papers describing new methodologies will also be considered. A series of short papers on the same or related topic is not appropriate for Current Microbiology.

Current impact factor: 1.36

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 1.359
2012 Impact Factor 1.52
2011 Impact Factor 1.815
2010 Impact Factor 1.51
2009 Impact Factor 1.33
2008 Impact Factor 1.33
2007 Impact Factor 1.167
2006 Impact Factor 1.007
2005 Impact Factor 1.059
2004 Impact Factor 1.075
2003 Impact Factor 1.125
2002 Impact Factor 1.21
2001 Impact Factor 1.059
2000 Impact Factor 1.029
1999 Impact Factor 1.165
1998 Impact Factor 1.094
1997 Impact Factor 1.011
1996 Impact Factor 1.092
1995 Impact Factor 0.962
1994 Impact Factor 0.983
1993 Impact Factor 1.087
1992 Impact Factor 0.94

Impact factor over time

Impact factor

Additional details

5-year impact 1.65
Cited half-life 7.20
Immediacy index 0.19
Eigenfactor 0.01
Article influence 0.45
Website Current Microbiology website
Other titles Current microbiology (Online)
ISSN 1432-0991
OCLC 41223110
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Springer Verlag

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
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  • Conditions
    • Author's pre-print on pre-print servers such as
    • Author's post-print on author's personal website immediately
    • Author's post-print on any open access repository after 12 months after publication
    • Publisher's version/PDF cannot be used
    • Published source must be acknowledged
    • Must link to publisher version
    • Set phrase to accompany link to published version (see policy)
    • Articles in some journals can be made Open Access on payment of additional charge
  • Classification
    ​ green

Publications in this journal

  • Jun Zhang · Shengguo Zhao · Yangdong Zhang · Peng Sun · Dengpan Bu · Jiaqi Wang
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    ABSTRACT: Analysis of the full-length 18S rRNA gene sequences of rumen ciliates is more reliable for taxonomical classification and diversity assessment than the analysis of partial hypervariable regions only. The objective of this study was to develop new oligonucleotide primers targeting the full-length 18S rRNA genes of rumen ciliates, and to evaluate the effect of different sources of dietary fiber (corn stover or a mixture of alfalfa hay and corn silage) and protein (mixed rapeseed, cottonseed, and/or soybean meals) on rumen ciliate diversity in dairy cows. Primers were designed based on a total of 137 previously reported ciliate 18S rRNA gene sequences. The 3'-terminal sequences of the newly designed primers, P.1747r_2, P.324f, and P.1651r, demonstrated >99 % base coverage. Primer pair D (P.324f and P.1747r_2) was selected for the cloning and sequencing of ciliate 18S rRNA genes because it produced a 1423-bp amplicon, and did not amply the sequences of other eukaryotic species, such as yeast. The optimal species-level cutoff value for distinguishing between the operational taxonomic units of different ciliate species was 0.015. The phylogenetic analysis of full-length ciliate 18S rRNA gene sequences showed that distinct ciliate profiles were induced by the different sources of dietary fiber and protein. Dasytricha and Entodinium were the predominant genera in the ruminal fluid of dairy cattle, and Dasytricha was significantly more abundant in cows fed with corn stover than in cows fed with alfalfa hay and corn silage.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0898-3
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    ABSTRACT: Drug-resistant TB poses a major threat to control of TB worldwide. Despite progress in the detection of Multidrug-resistant TB (MDR-TB) cases, a major diagnostic gap remains: 55 % of reported TB patients estimated to have MDR-TB were not detected in 2013. MDR-TB antigens were conjugated to CNBr-activated Sepharose 4B. Specific polyclonal antibodies against MDR-TB Ags were prepared in rabbits using two boosted injections of the MDR-TB antigen. The antibodies were purified and treated with susceptible TB to remove any non-specific and cross-reactive antibodies. In the present study, comparative analysis of electrophoretic pattern of different antigens of INH/RIF-resistant TB were studied for identifying protein profiles. A RIF-resistant TB antigen was shown here to have different protein profiles from INH-resistant TB isolate. The results of Western blotting analysis showed that in the RIF- and INH-resistant antigenic fractions some bands of 14.4 and 45 kDa as immunogenic were common. Moreover, four bands of RIF-resistant TB antigen fractions (16, 19, 21, and 45 KDa) and one band of INH-resistant TB (about 26 KDa) were detected as diagnostic antigens. This study suggests that the Western blot is an accurate test to survey INH- and RIF-resistant TB antigens of M. tuberculosis infection. These findings indicate that MDR-TB diagnosis (based on Ag detection) could be useful in the identification of disease stages that precede symptomatic and microbiologically positive TB, such as subclinical and incipient TB.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0891-x
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    ABSTRACT: Phospholipase C (PLC) plays important roles in regulating various biological processes in eukaryotes. Currently, little is known about the function of PLC in filamentous fungi, especially the plant pathogenic fungi. Fusarium graminearum is the causal agent of Fusarium head blight in many cereal crops. BLAST search revealed that Fusarium genome contains six FgPLC genes. Using quantitative RT-PCR, different FgPLC gene expressions in mycelia were analyzed. To investigate the role of FgPLC in F. graminearum biology, a pharmacological study using a known inhibitor of PLC (U73122) was conducted. Results showed that inhibition of FgPLC resulted in significant alterations of mycelial growth, conidiation, conidial germination, perithecium formation, and expressions of Tri5 and Tri6 genes. As expected, the treatment of F. graminearum with U73343, an inactive analog of U73122, showed no effect on F. graminearum biology. Our results suggested strongly that FgPLC plays important roles in F. graminearum growth and development.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0901-z
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    ABSTRACT: A filamentous actinomycete, designated strain ZX01(T), was isolated from forest soil around Kanas Lake of China. A polyphasic taxonomic study was carried out to establish the status of strain ZX01(T). Chemical and morphological properties of the isolate were similar to those of species of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain ZX01(T) in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited the highest sequence similarities to Streptomyces lavendofoliae NBRC 12882(T) (99.1 %), S. luridus NBRC 12793(T) (99.0 %), S. lavendulocolor NBRC 12881(T) (99.0 %), S. gobitricini NBRC 15419(T) (99.0 %), and S. roseolilacinus NBRC 12815(T) (98.9 %). Low DNA-DNA relatedness values of 54.0, 50.0, 60.0, 66.7, and 50.4 %, respectively, were found between strain ZX01(T) and corresponding strains above. A number of phenotypic properties also enabled the isolate to be differentiated from related species of the genus Streptomyces. Therefore, it is proposed that strain ZX01(T) should be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces kanasensis sp. nov. The type strain is ZX01(T) (= CGMCC 4893(T) =JCM 30232(T)).
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0900-0
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    ABSTRACT: The aim of the study was to isolate and characterize potential multi-metal-resistant bacteria from ore soils. A total of three bacteria were isolated and assayed for resistance to arsenic (As), copper (Cu), and lead (Pb). Isolate Halomonas sp. MG exhibited maximum resistance to 1000 mg Pb/L, 800 mg As/L, and 500 mg Cu/L and it was identified as Halomonas sp. based on the partial 16S rDNA sequences. The metal(loid)s resistance mechanisms were further confirmed by amplification of arsC (As) copAU (Cu), and pbrT (Pb) genes. Biological transmission electron micrographs and XRD studies showed that the isolate Halomonas sp. MG transformed and/or biomineralized the metals either intracellularly or extracellularly. These results suggest that the isolate could be used as a potential candidate for the bioremediation of As, Cu, and Pb.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0897-4
  • Current Microbiology 08/2015;
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    ABSTRACT: From July to October 2013, nine out of 40 Acanthodactylus nilsoni collected from Western Iran, showed clinical signs of dermatitis in the dorsal and ventral surface of neck and fingers. Therefore, the aim of this survey was to identify the fungal flora colonizing the skin of A. nilsoni using morphological and molecular studies. Nine isolates of Fusarium were obtained from infected lizard samples and identified as Fusarium proliferatum through study of morphological characters. In the present study, selected F. proliferatum isolates (USMGFSC 230-112, USMGFSC 186-113, and USMGFSC 33-114) were examined and phylogenetically analysed on the basis of partial sequences of the tef1 and tub2 genes. Sequence analysis supported the morphological data, and all isolates were placed within F. proliferatum species. This is the first report on morphological and molecular identification of F. proliferatum isolated from lizards' dermatitis in Iran.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0892-9
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    ABSTRACT: The increasing rise in the production of meat around the world causes a significant generation of agro-industrial waste-most of it with a low value added. Fatty wastes have the potential of being converted into biodiesel, given the overcome of technological and economical barriers, as well as its presentation in solid form. Therefore, the aim of this work was to investigate the capacity of Staphylococcus xylosus strains to modify the chemical structure of chicken fatty wastes intending to reduce the melting points of the wastes to mild temperatures, thereby breaking new ground in the production of biodiesel from these sources in an economically attractive and sustainable manner. The effects in time of fermentation and concentration of the fat in the medium were investigated, assessing the melting point and profile of fatty acids. The melting temperature showed a decrease of approximately 22 °C in the best operational conditions, due to reduction in the content of saturated fatty acids (high melting point) and increase of unsaturated fatty acids (low melting point).
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0890-y
  • Current Microbiology 08/2015; DOI:10.1007/s00284-015-0894-7
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    ABSTRACT: A novel Pseudoalteromonas marina bacteriophage, PH101, specifically infecting Pseudoalteromonas BH101 was isolated from the water sample of the Yellow Sea of China using the agar overlay method. 16S rDNA sequence identification was used to identify the host bacteria. Efficiency of infection, multiplicity of infection value, morphological characterization, one-step growth curve, and host range of the bacteriophage were determined. Purified PH101 genomic DNA was extracted and its genome was completely sequenced and analyzed. The phage morphology showed that PH101 belongs to the Myoviridae family with a head of 60 nm in diameter and a tail of 40 nm with a tail fiber of 10-20 nm. Microbiological characterization demonstrated that phage PH101 is stable at a wide range of temperatures (0-70 °C) and showed acid and alkaline resistance (pH 3-12). The one-step growth curve showed a latent period of about 20 min, a rise period of 20 min, and a burst size of about 31.6 virions. The genome sequencing and bioinformatic analysis shows that phage PH101 was a novel bacteriophage which was found to consist of a linear, double-stranded 131,903-bp DNA molecule with a GC content of 37.36 % and 228 putative open reading frames without RNA, which were classified into seven functional groups, including phage structure, adsorption, packaging, gene transfer protease, terminase, DNA binding, and regulation.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0896-5
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    ABSTRACT: Gram-negative plant pathogenic bacteria regulate specific gene expression in a population density-dependent manner by sensing level of Acyl-Homoserine Lactone (HSL) molecules which they produce and liberate to the environment, called Quorum Sensing (QS). The production of virulence factors (extracellular enzyme viz. cellulase, pectinase, etc.) in Pectobacterium carotovorum subsp. carotovorum (Pcc) is under strong regulation of QS. The QS signal molecule, N-(3-oxohexanoyl)-L-Homoserine Lactone (OHHL) was found as the central regulatory system for the virulence factor production in Pcc and is also under strict regulation of external environmental temperature. Under seven different incubation temperatures (24, 26, 28, 30, 33, 35, and 37 °C) in laboratory condition, highest amount of OHHL (804 violacein unit) and highest (79 %) Disease Severity Index (DSI) were measured at 33 °C. The OHHL production kinetics showed accumulation of highest concentration of OHHL at late log phase of the growth but diminution in the concentration occurred during stationary phase onwards to death phase. At higher temperature (35 and 37 °C) exposure, OHHL was not at detectable range. The effect of temperature on virulence factor production is the concomitant effect of HSL production and degradation which justifies less disease severity index in cross-inoculated tomato fruits incubated at 35 and 37 °C. The nondetection of the OHHL in the elevated temperature may because of degradation as these signal molecules are quite sensitive and prone to get degraded under different physical factors. This result provides the rationale behind the highest disease severity up to certain elevated temperature and leaves opportunities for investigation on mutation, co-evolution of superior plant pathogen with more stable HSL signals-mediated pathogenesis under global warming context.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0888-5
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    ABSTRACT: The formation of fruiting body in Volvariella volvacea is affected by endogenous genes and environmental factors. However, its regulation at a molecular level is still poorly understood. To study the genes involved in the formation of fruiting body, we cloned a new regulator of the G protein signaling (RGS) encoding gene (rgs) from V. volvacea. Phylogenetic analysis showed that RGS in V. volvacea and other basidiomycete RGS proteins from Schizophyllum commune and Coprinus cinereus belong to the same clade. In addition, we assayed intracellular cAMP content in the three developmental stages (mycelium, fruiting body primordia, and button). We also found that the expression of rgs was highly positively correlated to the content of intracellular cAMP during fruiting body formation. The conserved protein sequences and expression of rgs, together with high concent of cAMP at primordia tissue, suggested that rgs gene and cAMP may play a crucial role in fruiting body formation in V. volvacea.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0885-8
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    ABSTRACT: A diverse group of soil bacteria known as plant growth promoting rhizobacteria (PGPR) is able to inhabit the area close to plant roots and exert beneficial effects on plant growth. Beneficial interactions between rhizospheric bacteria and plants provide prospects for isolating culturable PGPR that can be used as bio-fertilizers for sustainable crop production in communities that cannot easily afford chemical fertilizers. This study was conducted with the aim of isolating rhizospheric bacteria from grasses along the Kavango River and screening the bacterial isolates for plant growth promoting characteristics. The bacteria were isolated from rhizospheres of Phragmites australis, Sporobolus sp., Vetiveria nigritana, Pennisetum glaucum and Sorghum bicolor. The isolates were screened for inorganic phosphate solubilization, siderophore production and indole-3-acetic acid (IAA) production. The nitrogen-fixing capability of the bacteria was determined by screening for the presence of the nifH gene. Up to 21 isolates were obtained from P. australis, Sporobolus sp., S. bicolor, P. glaucum and V. nigritana. The genera Bacillus, Enterobacter, Kocuria, Pseudomonas and Stenotrophomonas, identified via 16S rDNA were represented in the 13 PGPR strains isolated. The isolates exhibited more than one plant growth promoting trait and they were profiled as follows: three phosphate solubilizers, four siderophore producers, eight IAA producing isolates and five nitrogen-fixers. These bacteria can be used to develop bio-fertilizer inoculants for improved soil fertility management and sustainable production of local cereals.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0886-7
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    ABSTRACT: The genes opcA and zwf are located close to each other in most of cyanobacterial strains and the mutations in the opcA gene were reported to lose most of G6PDH activity. One of the reasons suggested for this loss was the polar effect of the mutation on expression of the zwf gene in the same operon and the other was absence of the OpcA polypeptide necessary for the catalytic activity of G6PDH. Synechocystis sp. PCC 6803 exhibits a gene organisation in which opcA and zwf are far away from each other and is ideal for analysis of an opcA mutation alone. In this study, an opcA single mutant and an opcA-zwf double mutant were constructed and effects of the opcA mutation on G6PDH activity and dark viability were then investigated. Contrary to the previous observations, no negative effect of the mutation on G6PDH activity was found under the optimal substrate concentrations. However, when one of the substrates, G6P or NADP, was reduced gradually, G6PDH activity in the mutant cells decreased faster than the wild types. Our results indicated that an opcA mutation did not affect G6PDH activity severely when zwf and opcA were in different operons. Similarly, dark viability of the opcA single and the zwf-opcA double mutants did not exhibit meaningful difference from the wild type.
    Current Microbiology 08/2015; DOI:10.1007/s00284-015-0889-4
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    ABSTRACT: The occurrence, seasonal variation and genetic diversity of Campylobacter spp. in pigeons and crows over a 1-year period were evaluated. Campylobacter spp. were isolated from 166 (34.6 %) out of 480 wild bird faecal samples. The occurrence of Campylobacter spp. in faecal samples was higher among crows (39.2 %) than pigeons (30.0 %), (P < 0.05). Campylobacter jejuni was the most common species detected among wild bird faecal samples (98.2 %). Meanwhile, Campylobacter coli prevalence in wild bird faecal samples was low-6 %. The Simpson's diversity index of C. jejuni flaA RFLP types was lower in pigeons (D = 0.88) compared with C. jejuni isolates detected in crows (D = 0.97). Obtained results revealed that C. jejuni are widely prevalent among crows and pigeons, indicating these wild birds as potential infection sources to humans. Further studies are required to determine crows and pigeons role in zoonotic transmission of Campylobacter.
    Current Microbiology 07/2015; DOI:10.1007/s00284-015-0881-z
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    ABSTRACT: To well understand the community structure and composition of mesophilic microorganisms in anaerobic system fed with PTA wastewater, an up-flow anaerobic fixed bed reactor was continuously run at 33 and 37 °C for 75 and 60 days, respectively. Both fluorescence in situ hybridization analysis and 454-pyrosequencing were applied to investigate the microbial distinction within mesophilic ranges. A preferable performance was achieved at 37 than 33 °C. The taxonomic complexities of two samples were further compared at phylum, class, and genus levels. Notably, microbial diversity differed a lot and the change of populations was observed mainly in the shared OTUs. Genus level analysis showed that when temperature was increased to 37 °C, the abundance of Thauera and Hydrogenophaga (β-Proteobacteria) decreased by 93.75 and 61.47 %, respectively, whereas that of Syntrophorhabdus (δ-Proteobacteria) increased from 4.93 to 16.01 %. Furthermore, the dominant archaeal Methanobacterium at both temperatures indicated the prevailing contribution of hydrogenotrophic methanogens in mesophilic anaerobic system.
    Current Microbiology 07/2015; DOI:10.1007/s00284-015-0884-9
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    ABSTRACT: This study was conducted to determine the prevalence of Arcobacter spp. in various water sources of stream, creek, pond, and drinking water in Kars and surrounding areas. A total of 113 water samples including 19 samples from creeks, 49 from streams, 10 from ponds, and 35 from drinking water samples collected from different regions were examined for presence of Arcobacter spp. by cultural methods. Arcobacter spp. were isolated from 14 (12.38 %) samples including 5 (26.31 %) creek and 9 (18.36 %) stream water samples and all were identified as Arcobacter butzleri by multiplex PCR. No agent was isolated from pond and drinking water samples. The results of this study demonstrated that creek and stream waters are contaminated by this agent showing high potential risk of Arcobacter species to be transmitted to humans and animals and in the contamination of food. It is concluded that water sources should also be considered as a factor not only carrying agents but also as a primary source of the infection.
    Current Microbiology 07/2015; DOI:10.1007/s00284-015-0883-x