Saudi Journal of Biological Sciences

Description

  • Impact factor
    0.00
  • 5-year impact
    0.00
  • Cited half-life
    0.00
  • Immediacy index
    0.00
  • Eigenfactor
    0.00
  • Article influence
    0.00
  • ISSN
    1319-562X

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Despite the importance of bryophyte-associated microorganisms in various ecological aspects including their crucial roles in the soil-enrichment of organic mass and N2 fixation, nonetheless, little is known about the microbial diversity of the bryophyte phyllospheres (epi-/endophytes). To get insights into bacterial community structures and their dynamics on the bryophyte habitats in different ecosystems and their potential biological roles, we utilized the 16S rRNA gene PCR-DGGE and subsequent phylogenetic analyses to investigate the bacterial community of eight bryophyte species collected from three distinct ecosystems from western Japan. Forty-two bacterial species belong to γ-proteobacteria and firmicutes with 71.4% and 28.6%, respectively, were identified amongst 90 DGGE gel bands population. These DGGE-bands were assigned to 13 different genera with obvious predomination to the genus Clostridium with 21.4% from the total bacterial community. These analyses provide new insights into bryophyte-associated bacteria and their relations to the ecosystems.
    Saudi Journal of Biological Sciences 08/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study is designed to demonstrate the ovarian surface epithelial cells' (OSE) estrogen receptor α (ERα) and progesterone receptor (PR) during pregnancy and estrous cycle in rat. Moreover, determination of the levels of plasma progesterone, estradiol, FSH and LH was also made. The levels of plasma progesterone, estradiol, FSH and LH concentrations were determined on days 7 (n = 5), 14 (n = 5), and 21 (n = 5) of pregnancy in three groups of rats and during the estrous cycle (n = 5) using an ELISA kit. Immunohistochemical method for PR and ERα expressions was also made on the ovary. During pregnancy, FSH and LH remained low except at term when LH levels began to increase from 16 ng/ml to 47 ng/ml. Progesterone levels significantly exceeded estradiol values in all pregnant rats with a peak value of 202 ng/ml on day 14. Elevated progesterone levels were associated negatively with LH and estradiol levels during pregnancy. The levels of estradiol surged significantly on day 21. Immunohistochemistry of the ovary showed low levels of OSE cells staining positive for ERα expression. ERα positive cells were absent on day 7 and 14 of pregnancy, only day 21 recorded a very low percentage of immunostaining (0.5%) within the nuclei of OSE cells. On the contrary, immunostaining of PR was not observed within the nuclei of OSE cells in all groups of study. In conclusion, these results may suggest that the progesterone effect during pregnancy seems to be overriding the positive effect of estrogens on OSE cells. High progesterone levels may have a direct negative effect on gonadotropin production and thereby it might inhibit events leading to both follicular development and OSE proliferation. Understanding the factors affecting OSE proliferation may help elucidating the mechanism(s) of assisted diseases such as ovarian cancer.
    Saudi Journal of Biological Sciences 07/2014; 21(3):232-7.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Nanodiagnostics is the term used for the application of nanobiotechnology in molecular diagnosis, which is important for developing personalized cancer therapy. It is usually based on pharmacogenetics, pharmacogenomics, and pharmacoproteomic information but also takes into consideration environmental factors that influence response to therapy. Nanotechnology in medicine involves applications of nanoparticles currently under development, as well as longer range research that involves the use of manufactured nano-robots to make repairs at the cellular level. Nanodiagnostic technologies are also being used to refine the discovery of biomarkers, as nanoparticles offer advantages of high volume/surface ratio and multifunctionality. Biomarkers are important basic components of personalized medicine and are applicable to the management of cancer as well. The field of nano diagnostics raises certain ethical concerns related with the testing of blood. With advances in diagnostic technologies, doctors will be able to give patients complete health checks quickly and routinely. If any medication is required this will be tailored specifically to the individual based on their genetic makeup, thus preventing unwanted side-effects.
    Saudi Journal of Biological Sciences 04/2014; 21(2):109-117.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Aim & Background Heart-type fatty acid-binding proteins (H-FABP) which are detected within 2 to 3 hours of acute myocardial infarction are involved in uptake of free fatty acids in myocardium. Our aim in the present study is to compare window periods of H-FABP to high sensitivity troponin T (hs-Trop T) in acute ST elevation myocardial infarction (STEMI). Method 160 diagnosed STEMI patient’s serum samples are analysed for hs-Trop T and H-FABP. Different window periods of chest pain onset (< 3 hours, 3-6 hours and > 6hours) are compared with complications, in-hospital mortality and statistically analysed. Result From 160 patients, 53 (33%) cases are presented in <3 hours, 75 (47%) in 3 to 6, 32 (20%) after > 6 hours respectively. Accordingly sensitivity of hs-Trop T was 92%, 94%, 97% while H-FABP was 75%, 88%. and 84%, respectively. Overall sensitivity was 94% and 82% respectively. Statistically significant difference between mean hs-Trop T values with respect to window period < 3, 3-6 and >6 hours was (0.21ng/ml, 0.35 ng/ml, 0.80 ng/ml respectively, p value <0.0001). No significant difference in H-FABP values observed. Hs Trop T positively correlated with age (r =0.153, P = 0.05), window period (r = 0.363, P <0.0001), TIMI score (r = 0.208, P = 0.008), ejection fraction (r = 0.191, P =0.008), serum H-FABP (r = 0.229, P = 0.004), serum hs CRP (r = 0.326, p < 0.001). Statistically significant difference of mean hs Trop T values with or without in hospital mortality was (0.35ng/ml vs 0.85ng/ ml respectively, p = 0.008). No significant correlation to age, TIMI score, ejection fraction and hs CRP values for H-FABP. Conclusion It appears that hs-Trop T is more sensitive marker than H-FABP in early hours of AMI and higher hs-Trop T predicts increase in-hospital mortality.
    Saudi Journal of Biological Sciences 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The algae-induced odorous black water agglomerate (OBWA) is a phenomenon that water becomes black and emits odorous gas. It is an ecological and environmental problem that has occurred several times in Taihu, a large eutrophic shallow lake in China. In this study, the collected eutrophic water with different algae densities was used to simulate OBWA. The results revealed that the massive accumulation and death of algae was the substrate source for OBWA. When the algae density reached 1.0×108cells/L in the static and dark condition, at a constant high temperature (30±2°C), OBWA happened. There was a time difference between the water stinking and blackening with the stinking first. When the oxidation-reduction potential (ORP) value was between -250 and -50 mV, Dimethyl trisulfide (DMTS), the main contributor to the water stinking at the initial stage, and other odorous organics were produced. Water blackening was closely related to the increases of sulfide and dissolved Fe2+ concentration. When the ORP value was between -350 and -300mV, heavy metal containing sulfides such as FeS formed. Therefore, the condition when the water ORP value decreased to about -300mV was considered the precursor for OBWA formation.
    Saudi Journal of Biological Sciences 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Agro-industrial residues and cow dung were used as the substrate for the production of alkaline protease by Bacillus cereus strain AT. The bacterial strain Bacillus cereus strain AT produced a high level of protease using cow dung substrate (4813 ± 62 U g−1). Physiological fermentation factors such as the incubation time (72 h), the pH (9), the moisture content (120%), and the inoculum level (6%) played a vital role in the enzyme bioprocess. The enzyme production improved with the supplementation of maltose and yeast extract as carbon and nitrogen sources, respectively. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and zymogram analysis of the purified protease indicated an estimated molecular mass of 46 kDa. The protease enzyme was stable over a temperature range of 40–50 °C and pH 6–9, with maximum activity at 50 °C and pH 8. Among the divalent ions tested, Ca2+, Na+ and Mg2+ showed activities of 107 ± 0.7%, 103.5 ± 1.3%, and 104.6 ± 0.9, respectively. The enzyme showed stability in the presence of surfactants such as sodium dodecyl sulfate and on various commercially available detergents. The crude enzyme effectively de-haired goat hides within 18 h of incubation at 30 °C. The enzymatic properties of this protease suggest its suitable application as an additive in detergent formulation and also in leather processing. Based on the laboratory results, the use of cow dung for producing and extracting enzyme is not cumbersome and is easy to scale up. Considering its cheap cost and availability, cow dung is an ideal substrate for enzyme bioprocess in an industrial point of view.
    Saudi Journal of Biological Sciences 01/2014; 21(1):27–34.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Metabolomic profiling of different parts (leaves, flowers and pods) of Acacia species (Acacia nilotica, Acacia seyal and Acacia laeta) was evaluated. The multivariate data analysis such as principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) was used to differentiate the distribution of plant metabolites among different species or different organs of the same species. Acacia nilotica was characterized with high content of saponins and Acacia seyal was characterized with high content of proteins, phenolics, flavonoids and anthocyanins. Acacia laeta had higher content of carbohydrates than A. nilotica and A. seyal. On the basis of these results, total antioxidant capacity, DPPH free radical scavenging activity and reducing power of the methanolic extracts of studied parts were evaluated. Acacia nilotica and Acacia seyal extracts showed less inhibitory concentration 50 (IC50) compared to Acacia laeta extracts which means that these two species have the strongest radical scavenging activity where as A. laeta extracts have the lowest radical scavenging activity. Positive correlation between saponins and flavonoids with total antioxidant capacity and DPPH radical scavenging activity was observed. Based on these results, the potentiality of these plants as antioxidants was discussed.
    Saudi Journal of Biological Sciences 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study was designed to determine the effects of Ganoderma lucidum polysaccharides (GL-PS) on exhaustive exercise-induced oxidative stress in skeletal muscle tissues of mice. The mice were divided into four groups (three GL-PS administered groups and the control group). The control group was administered with distilled water and GL-PS administered groups were administered with GL-PS (50, 100 and 200 mg/kg body weight per day). After 28 days, the mice performed an exhaustive swimming exercise, along with the determination of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) activities and malondialdehyde (MDA) levels in the skeletal muscle of mice. The results showed that GL-PS could increase antioxidant enzymes activities and decrease the MDA levels in the skeletal muscle of mice. This study provides strong evidence that GL-PS supplementation possessed protective effects against exhaustive exercise-induced oxidative stress.
    Saudi Journal of Biological Sciences 01/2014; 21(2):119–123.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Secnidazole (α,2-Dimethyl-5-nitro-1H-imidazole-1-ethanol) is a highly effective drug against a variety of G+/G- bacteria but with significant side effects because it is being used in very high concentration. In this study, gold nanoparticles (GNPS) were selected as a vehicle to deliver secnidazole drug at the specific site with more accuracy which made the drug highly effective at substantially low concentrations. The as-synthesized GNPs were capped with Human Serum Albumin (HSA) and subsequently bioconjugated with secnidazole because HSA provides the stability and improves the solubility of the bioconjugated drug, secnidazole. The quantification of covalently bioconjugated secnidazole with HSA encapsulated on enzymatically synthesized GNPs was done with RP-HPLC having SPD-20 A UV/ VIS detector by using the C-18 column. The bioconjugation of GNPs with secnidazole was confirmed by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The bioconjugated GNPs were characterized by UV-VIS spectroscopy, TEM, Scanning Electron Microscopy (SEM) and DLS. Zeta potential confirmed the stability and uniform distribution of particles in the emulsion of GNPs. The separation of bioconjugated GNPs, unused GNPs and unused drug was done by gel filtration chromatography. The minimal inhibitory concentration of secnidazole–conjugated gold nanoparticles (Au–HSA-Snd) against Klebsiella pneumonia (NCIM No. 2957) and Bacillus cereus (NCIM No. 2156) got improved by 12.2 times and 14.11 times, respectively, in comparison to pure secnidazole. Precisely, the MIC of Au–HSA-Snd against Klebsiella pneumonia (NCIM NO. 2957) and Bacillus cereus (NCIM NO. 2156) were found to be 0.35 μg/ml and 0.43 μg/ml, respectively whereas MIC of the pure secnidazole drug against the same bacteria were found to be 4.3 μg/ml and 6.07 μg/ml respectively.
    Saudi Journal of Biological Sciences 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Salt-tolerant ecotypes (or cultivars, varieties etc.) of different plant species have been long known to evolve in nature. In the past few years, plant breeders have made significant achievements regarding salt tolerance in a number of potential crops using artificial selection. The aim of this work was to evaluate and screening of the natural sea water (Red sea) tolerance of 7 Saudi local (Baish, Jazan; 17.388086, 42.524070) cultivars of sorghum (Sorghumbicolor L., Moench; Poaceae) with respect to the performance of some physiological parameters such as germination, shoot and root development which could be recommended to local farmers and plant breeders. The shoot growth of the studied sorghum cultivars were significantly affected by the exposure to the sea water. Root growth was different among cultivars even when treated with normal water. The cultivar C3 (mix white and red seeds) was observed more salt tolerant and cultivar C4 (whitish seeds) was more salt sensitive on the basis of germination-ability and shoot development. Cultivar C3 was also observed to produce better seeds compared with the other cultivars. Results of this experiment can be useful to the local sorghum growing farmers or as genetic resource for the development of sorghum cultivars with improved germination under salt stress.
    Saudi Journal of Biological Sciences 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Poultry meat and its products are widely consumed by humans globally, however, Aeromonas infections in poultry has been reported in different parts of the world with devastating effects. This study was carried out to assess the antibiogram and biofilm forming potential of Aeromonas isolated from chicken faecal samples. Aeromonas isolates were screened for antibiotic susceptibility using antibiotics disc and biofilm producing potentials on abiotic surface. Nineteen isolates recovered from chicken feces were 100% sensitive to ciprofloxacin, gentamicin and the tetracyclines. About 53% of Aeromonas isolates were resistant to erythromycin and 47% resistant to streptomycin. Eight isolates (42.1%) were found to be moderate producers of biofilm, 31.6% (6/19) were weak producers of biofilm, 10.5% (2/19) were non biofilm producer while 15.8% (3/19) were strong producers. The present investigation shows a prevalence of potentially pathogenic Aeromonas strains in chicken faeces, suggesting potential group at risk for Aeromonas infection which could be dissemination to other animal or human with close contact and the wider community.
    Saudi Journal of Biological Sciences 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Cell cultures of Psoralea corylifolia L. were established from the leaf disc derived callus. The effect of different biotic elicitors prepared from the fungal extract (Aspergillus niger and Penicillium notatum), yeast extract and chitosan with different concentration was studied. The increased synthesis of psoralen in 16-day old cell cultures under 16 hours of light and 8 hours of dark period was studied. Elicitation of psoralen in Aspergillus niger elicitor treated cells was found 9 fold higher over control cells. Treating the cells with Penicillium notatum, yeast extract and chitosan elicitors lead four to sevenfold higher psoralen accumulation over control cells. The extract of Aspergillus niger at 1.0% v/v increased the significant accumulation of psoralen (9850 μg/g DCW) in the cultured cells. Our study clearly shows that all the elicitors had the potential to increase the accumulation of psoralen but the Aspergillus niger elicitor at 1.0% v/v induced maximum accumulation.
    Saudi Journal of Biological Sciences 01/2014;

Related Journals