Saudi Journal of Biological Sciences

Publisher: Elsevier

Current impact factor: 1.26

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 1.257
2013 Impact Factor 0.741

Additional details

5-year impact 0.00
Cited half-life 3.20
Immediacy index 0.24
Eigenfactor 0.00
Article influence 0.00
ISSN 1319-562X

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Publications in this journal

  • Saudi Journal of Biological Sciences 11/2015; 22(6). DOI:10.1016/S1319-562X(15)00231-4
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    ABSTRACT: Cassia italica Mill is an important medicinal plant within the family Fabaceae. Pot experiment was conducted to evaluate cadmium stress induced changes in physiological and biochemical attributes in C. italica with and without arbuscular mycorrhizal fungi (AMF). Cadmium stressed plant showed reduced chlorophyll pigment and protein content while AMF inoculation enhanced the chlorophyll and protein content considerably. AMF also ameliorated the cadmium stress induced reduction in total chlorophyll and protein contents by 19.30% and 38.29%, respectively. Cadmium stress enhanced lipid peroxidation while AMF inoculation reduced lipid peroxidation considerably. Increase in proline and phenol content was observed due to cadmium stress and AMF inoculation caused a further increase in proline and phenol content ensuring better growth under stressed conditions. AMF alone also enhanced proline and phenol content. Activity of antioxidant enzymes enhanced under cadmium treatment and AMF inoculation further enhanced their activity thereby strengthening the antioxidant system. Enhanced activities of antioxidants and increased accumulation of osmolytes help plants to avoid damaging impact of oxidative damage. The research has shown that AMF inoculation mitigated the negative impact of stress by reducing the lipid peroxidation and enhancing the antioxidant activity. The present study strongly supports employing AMF as the biological mean for enhancing the cadmium stress tolerance of C. italica.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.11.007
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    ABSTRACT: This study was carried out to determine the median lethal concentrations (LC50) of Zinc nanoparticles (ZnNPs) on Oreochromis niloticus and Tilapia zillii.The biochemical and molecular potential effect of ZnNPs (500μgL-1 and 2000μgL-1) on antioxidant system in the brain tissue of O. niloticus and T. zillii were invetigated. Four hundred fish were used for acute and sub-acute study. The ZnNPs LC50 was investigated in O. niloticus and T. zillii. The effect of 500 and 2000μgL-1 ZnNPs on brain antioxidant of O. niloticus and T. zillii was investigated. The result indicated that 69h LC50 was 5.5±0.6 and 5.6±0.4 for O. nilotica and T. zillii respectively. Fish exposed to 500 μgL-1 ZnNPs showed a significant increase of reduced glutathione (GSH), total glutathione (tGSH) levels, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activity and gene expression. On the contrary, malondialdehyde (MDA) levels significantly decreased. Meanwhile, Fish exposed to 2000μgL-1 ZnNPs showed a significant decrease of GSH, tGSH levels, SOD, CAT, GR, GPx and GSTactivity and gene expression. On the contrary, MDA levels significantly increased. It concluded that, the 96 h LC50 of ZnNPs was 5.5±0.6 and 5.6±0.4 for O. nilotica and T. zillii respectively. ZnNPs in exposure concentration of 2000μg/L induced a deleterious effect on brain antioxidant system of O. nilotica and T. zillii. In contrast, ZnNPs in exposure concentration of 500μgL-1 produced an inductive effect on brain antioxidant system of O. nilotica and T. zillii.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.021
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    ABSTRACT: Prediction of complex super-secondary structure is a key step for the study of tertiary structures of proteins. The strand-loop-helix-loop-strand (βαβ) motif is an important complex super-secondary structure in proteins. Many functional sites and active sites often occur in the polypeptides of βαβ motifs. Therefore, the accurate prediction of βαβ motifs is very important to recognizing protein tertiary structure and the study of protein function. In this study, the βαβ motif dataset was first constructed by using DSSP package. A statistical analysis was then performed on βαβ motifs and non-βαβ motifs. The target motif was selected, and the length of the loop-α-loop varies from 10 to 26 amino acids. The ideal fixed-length pattern was 32 amino acids. A Support Vector Machine algorithm was developed for predicting βαβ motifs by using the sequence information, the predicted structure and function information to express the sequence feature,. The overall predictive accuracy of 5-fold cross-validation and independent test were 81.7% and 76.7%, respectively. The Matthew’s correlation coefficient of the 5-fold cross-validation and independent test are 0.63 and 0.53, respectively. The results demonstrate that the proposed method is an effective approach for predicting βαβ motifs and can be used for structure and function studies of proteins.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.005
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    ABSTRACT: Illicium verum, whose extractives can activate the demic acquired immune response, is an expensive medicinal plant. However, the rich extractives in I. verum biomass were seriously wasted for the inefficient extraction and separation processes. In order to further utilize the biomedical resources for the good acquired immune response, the four extractives were obtained by SJYB extraction, and then the immunology moleculars of SJYB extractives were identified and analyzed by GC–MS. The result showed that the first-stage extractives contained 108 components including anethole (40.27%), 4-methoxy-benzaldehyde (4.25%), etc.; the second-stage extractives had 5 components including anethole (84.82%), 2-hydroxy-2-(4-methoxy-phenyl)-n-methyl-acetamide (7.11%), etc.; the third-stage extractives contained one component namely anethole (100%); and the fourth-stage extractives contained 5 components including cyclohexyl-benzene (64.64%), 1-(1-methylethenyl)-3-(1-methylethyl)-benzene (17.17%), etc. The SJYB extractives of I. verum biomass had a main retention time between 10 and 20min what’s more, the SJYB extractives contained many biomedical moleculars, such as anethole, eucalyptol, [1S-(1α,4aα,10aβ)]-1,2,3,4,4a,9,10,10a-octahydro-1,4a-dimethyl-7-(1-methylethyl)-1-phenanthrenecarboxylic acid, stigmast-4-en-3-one, γ-sitosterol, and so on. So the functional analytical results suggested that the SJYB extractives of I. verum had a function in activating the acquired immune response and a huge potential in biomedicine.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.027
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    ABSTRACT: Surface reactivity of bioactive ceramics contributes in accelerating bone healing by anchoring osteoblast cells and the connection of the surrounding bone tissues. The presence of silicon (Si) in many biocompatible and bioactive materials has been shown to improve osteoblast cell adhesion, proliferation and bone regeneration due to its role in the mineralisation process around implants. In this study, the effects of Si-biphasic calcium phosphate (Si-BCP) on bioactivity and adhesion of human osteoblast (hFOB) as an in vitro model have been investigated. Si-BCP was synthesised using calcium hydroxide (Ca(OH)2) and phosphoric acid (H3PO4) via wet synthesis technique at Ca/P ratio 1.60 of material precursors. SiO2 at 3wt% based on total precursors was added into apatite slurry before proceeding with the spray drying process. Apatite powder derived from the spray drying process was pressed into discs with Ø 10mm. Finally, the discs were sintered at atmospheric condition to obtain biphasic hydroxyapatite (HA) and tricalcium phosphate (TCP) peaks simultaneously and examined by XRD, AFM and SEM for its bioactivity evaluation. In vitro cell viability of L929 fibroblast and adhesion of hFOB cell were investigated via AlamarBlue® (AB) assay and SEM respectively. All results were compared with BCP without Si substitution. Results showed that the presence of Si affected the material’s surface and morphology, cell proliferation and cell adhesion. AFM and SEM of Si-BCP revealed a rougher surface compared to BCP. Bioactivity in simulated body fluid (SBF) was characterised by pH, weight gain and apatite mineralisation on the sample surface whereby the changes in surface morphology were evaluated using SEM. Immersion in SBF up to 21days indicated significant changes in pH, weight gain and apatite formation. Cell viability has demonstrated no cytotoxic effect and denoted that Si-BCP promoted good initial cell adhesion and proliferation. These results suggest that Si-BCP’s surface roughness (164nm) was significantly higher than BCP (88nm), thus enhancing the adhesion and proliferation of the osteoblast.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.024
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    ABSTRACT: Rhazya stricta Decne is an important medicinal species used in indigenous medicinal herbal drugs to cure various diseases in South Asia (Pakistan, India and Afghanistan) and in the Middle East (e.g. Saudi Arabia, Qatar, United Arab Emirates (UAE), Iran and Iraq). Some of its alkaloids have been reported to have anticancerous properties. The aim of our study is to examine the morphological and taxonomical parameters for Rhazya stricta in the Saudi Arabia; concentrations and distributions of some secondary metabolites; and also to determine the antioxidant and free radical scavenging activity. The results of present study showed that there was no influence of environment on the structure of stomata and trichomes as studying species with Rhazya stricta. In conclusion our study shows no trichomes on leaf of R. stricta it is glabrous, whereas, variations between many secondary metabolites such as flavonoids and phenolic compounds occurred in response to change in climatic conditions.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.017
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    ABSTRACT: This study aimed to characterize eight accessions of Vicia narbonensis L. originated from different Mediterranean countries. The cytology of these species is rarely known despite the fact of its great socio-economical and ecological interest in these arid and semi-arid zones. This work aimed mainly to characterize the karyotype, morphological pod and seed traits of the species. Karyotypes of all accessions were similar to a diploid number of 2n=2x=14. All the accessions have submetacentric chromosomes with a secondary constriction attached to the long arm of pair VII close to the centromere. Variation in chromosomes size was observed; it ranged from 5.86 μm to 7.62 μm. Indices of karyotype asymmetry were calculated as the total form percentage (TF%) and (Syi) which ranged from 33.75% to 35.42% and from 51.01% to 54.85%, respectively. The predominance of submetacentric chromosomes indicated that the karyotype is symmetrical and can be considered as primitive. However, the analysis of quantitative parameters measured on pods and seeds showed a significant variation between accessions. A relationship between centromeric index and the pod beak length was found. Estimation of phenotypic diversity using the Shannon diversity index (H’) showed that the length, the seed color and the number of seeds per pod are the most polymorphic traits with respectively, H’= 0.92, 0.80 and 0.83. Cluster analysis of karyological, pod and seed traits showed four groups of accessions. This clustering is partially due to the geographical origin of the studied accessions. The variation in chromosome size, pod and seed traits could offer potentially valuable genetic resources for the improvement of Vicia narbonensis which is considered as neglected and underutilized crop species (NUCS).
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.11.003
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    ABSTRACT: As one of the dominant plantations in north and central China, poplar was considered as the uppermost wood raw materials, however, the chemical constituents of poplar wood weren’t effectively used by high added value. Therefore, the molecules of wood extractives in Populus lasiocarpa and Populus tomentosa were extracted and studied to further utilize the bio-resources. The results showed that the LD-010, LD-021, LD-150, LD-174 wood extractives were identified as having 3, 24, 3 27 components, respectively. P. lasiocarpa wood was fit to extract 2,4-hexadiyne, 1,3,3-trimethyl-2-hydroxymethyl-3,3-dimethyl-4-(3-methylbut-2-enyl)-cyclohexene, and P. tomentosa wood was fit to extract 1,5-hexadien-3-yne, (all-E)-2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene. So the extractives of poplar wood contained rich and rare drug and biomedical activities.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.026
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    ABSTRACT: This paper studies the global behaviors of a nonlinear autonomous neutral delay differential population model with impulsive perturbation. This model may be suitable for describing the dynamics of population with long larval and short adult phases. It is show that the system may have global stability of the extinction and positive equilibria, or grow without bound under some condition.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.018

  • Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.029
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    ABSTRACT: To prepare Coenzyme Q10 (CoQ10) long-circulating liposomes, and establish the quality standard to determine the content and entrapment efficiency. CoQ10 long-circulating liposomes were prepared by film dispersion method, HPLC assay for determination of CoQ10 was developed. Free drugs and liposomes were separated using protamine aggregation method and entrapment efficiency was determined. The liposomes were homogeneous and the mean diameter was 166.0 nm, Zeta potential was -22.2 mV. The content and entrapment efficiency of CoQ10 were 98.2% and 93.2% for the three batches of liposomes, respectively. The lyophilized form of liposomes prepared by freeze-drying showed stable quality characteristics during storage. The formulation and preparative method can be used to prepare CoQ10 long-circulating liposomes with high entrapment efficiency and high quality, the determination method of drug content and entrapment efficiency were effective and rapid and can be used to quality evaluation for liposomes.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.025
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    ABSTRACT: Health consciousness has increased the desire of people around the world to consume functional foods. Omega-3 essential fatty acids are one amongst these beneficial and important health supplements without which a general predisposition to degenerative and stress related disorders can occur. Saudi Arabia has shown an alarming increase in obesity(Al-Nozha, Al-Mazrou et al., 2005),diabetes(Alqurashi, Aljabri et al., 2011), and cardiovascular disease (Al-Nozha, Arafah et al., 2004) in the last few decades mainly due to nutritional transitions and lifestyle alterations (Amuna and Zotor, 2008). Lack of nutrient dense foods and the prevailing food related disorder of obesity(Popkin, 2001; Prentice, 2014) especially render egg as a choice food to be value-added for attaining nutritional security in Saudi Arabia and in effect reverse the increasing incidences of lifestyle diseases. Nutritional intervention through a commonly consumed food product would be an important step in improving the health of the people, and reducing health care costs. As eggs are a frequently consumed food item in Saudi Arabia, enriching them with omega-3 fatty acids would be an excellent way to alleviate the existing problems. A significant deposition of omega-3 fatty acids in the eggs was observed when the diet of hens was supplemented with omega-3 fatty acids from either flaxseed or fish oil source. Inadequacy of omega-3 fatty acids could thus be rectified by producing omega-3 enriched eggs from chicken supplemented with flaxseed or fish oil source, and thus contribute towards better health choice to the consumer.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.11.001
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    ABSTRACT: The aim of this study was to evaluate the use of dried marine seaweed, Gracilaria arcuata for the first time as dietary ingredient in partial substitution of fishmeal on the growth performance, feed utilization and body composition of African catfish, Clarias gariepinus. Four experimental diets were formulated: D1 as a control group; D2; D3 and D4 which included 10%, 20% and 30% G. arcuata meal respectively. One hundred and eighty African catfish weighing 9.62±0.42g, (mean±SE) was divided into four groups corresponding to the different feeding regimes. The final body weight of the fishes showed significant differences (P<0.05) between the control (D1); D2 and other treated groups D3 and D4, with weights of 66.98, 59.60, 47.34 and 30.73g recorded for D1, D2, D3 and D4, respectively. Significant differences (P<0.05) were also evident in weight gain, specific growth rate, and feed utilization between treatment and control groups. However, no significant differences (P>0.05) were observed between the control group and fishes fed D2 for all previous parameters. Protein productive value, protein efficiency ratio, daily dry feed intake and total feed intake were also significantly lower in fish fed with a diet containing G. arcuata than in the control group and D2 which contains 10% of G. arcuata. Overall, the results of the experiment revealed that African catfish fed a diet with G. arcuata included in 20% and 30% levels showed poorer growth and feed utilization than the control group and D2. However, the study recommended that C. gariepinus can accept this ingredient up to 10% in their diets. More defined experiments therefore seem to be necessary in order to determine the maximum level of this marine seaweed in diets with amino acid supplementation for African catfish.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.11.006
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    ABSTRACT: To discuss the cold resistance performance of different Herba Rhodiolae and successfully transplant Herba Rhodiolae to Gansu plateau area for nursing, domestication and planting, this paper systematically studies six physiological and biochemical features of Rhodiola kirilowii, Rhodiola algida, Rhodiola crenulata and Herba Rhodiolae that are closely associated with cold resistance feature and concludes that the cold resistance capability of Rhodiola kirilowii. In the selected six main indexes of the Herba Rhodiolae, the POD, SOD and CAT activity and MDA and Pro content in the leaf are the main physiological and biochemical indexes to indicate the cold resistance performance of four Herba Rhodiolae seedlings and can be regarded as the preliminary indexes to assess the winter performance of Herba Rhodiolae. The research work will provide the theoretical basis for the wild variants of Herba Rhodiolae and GAPJ base construction.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.10.007
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    ABSTRACT: Polyaspartic acid (PASP) hydrogel is an important and widely applied water-retaining agent, thanks to its special space network structure which contains a carboxyl group attached on the side chain. In this study, the PASP hydrogel with high water absorption rate (300-350 g H2O/g hydrogel) was developed and adopted to transplant Xanthoceras sorbifolia seedlings in the ecological restoration project of Mount Daqing National Nature Reserve. Transplantation experiments showed that the survival rate and leaf water content index for Xanthoceras sorbifolia seedlings were increased by 8-12% and 4-16%, respectively. Additionally, compared with the counterpart without PASP hydrogel, the value of chlorophyll fluorescence that was considered as one of the most important indicators of plant physiology, was significantly improved with the addition of PASP hydrogel. The PASP hydrogel displays a promising future for the applications of increasing the survival rate and simultaneously alleviating the drought stress effects on the pioneer plants in the arid and semi-arid areas.
    Saudi Journal of Biological Sciences 11/2015; DOI:10.1016/j.sjbs.2015.08.016