Journal of Animal and Feed Sciences (J ANIM FEED SCI )

Description

Impact factor 0.59

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: ABSTRACT. Autoclaving is the current method of choice for sterilization of diets for specified-pathogen-free (SPF) laboratory animals, but this process may negatively affect the nutrient content (e.g., protein, vitamin) of the diets. Two natural-ingredient diets supplemented with either soyabean meal containing phytoestrogens (S) or with casein (C), and a commercial autoclavable (SN) diet, were autoclaved at 121°C for 20 min (T1) or at 134°C for 10 min (T2). Chemical composition, selected water- and fat-soluble vitamins, and acrylamide were analysed in all non-autoclaved (T0) and autoclaved diets, whereas in S diets, oestrogenic isoflavones were also determined. Autoclaving affected most protein bound to NDF macronutrient contents only to a small degree, except those of NDF and N-NDF. The contents of NDF and N-NDF were increased by T2 and T1; the change was the largest in the SN and the smallest in the C diet. Losses of particular vitamins differed among the diets and treatments. Among the water-soluble vitamins, thiamine, riboflavin and pyridoxine were the least affected, whereas calcium pantothenate was lowered by T1 and T2. Among fat-soluble vitamins, vitamin E was the most stable, while vitamins A and D, the least stable, but even the highest vitamin losses did not exceed 50%. The acrylamide concentration increased more in diets autoclaved at T2 than at T1 and in the S and C diets than in SN. Autoclaving the S diet increased the daidzin and genistin contents and slightly reduced the total isoflavone content. It is concluded that the effects of autoclaving different diets are not uniform, but longer autoclaving at a lower temperature (T1) is less detrimental than shorter treatment at a higher temperature (T2), mainly because of the smaller increase in the NDF and N-NDF contents and acrylamide concentration.
    Journal of Animal and Feed Sciences 12/2014; 23(4):351-360.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this study was to evaluate two near-infrared reflectance spectroscopy (NIRS) applications and different laboratory methods for determining indigestible neutral detergent fibre (iNDF) and organic matter digestibility (OMD) of forage maize hybrids harvested at high latitudes. Different in situ and in vitro methods have been used in calibrations of NIRS for routine determinations of forage feed value in the Nordic countries. Swedish and Finnish NIRS calibrations of iNDF generated biased estimates of low precision of iNDF determined in situ. All methods used to predict OMD in forage maize systematically underestimated the observed OMD calculated from the in situ determined value of iNDF. Digestibility was determined with least precision based on the enzymatic in vitro procedure. Values of OMD based on the in vitro incubation in buffered rumen fluid displayed the smallest error among the laboratory methods. The NIRS calibration developed with the forage maize samples of this study, suggested that NIRS has a great potential to predict iNDF and OMD of forage maize accurately and precisely. It was concluded that NIRS predictions of OMD can be a promising tool for evaluating performance of forage maize hybrids at high latitudes if NIRS calibrations of iNDF including forage maize samples are available.
    Journal of Animal and Feed Sciences 09/2014; 23:269-278.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%–2.69%) and intermediate precision (1.36%–4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry.
    Journal of Animal and Feed Sciences 06/2014; 23:185–189.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%–2.69%) and intermediate precision (1.36%–4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry.
    Journal of Animal and Feed Sciences 06/2014; 23:185–189.