Biochemical and Biophysical Research Communications (Biochem Biophys Res Comm)

Publisher: Elsevier

Journal description

Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination (six weeks) of timely and significant experimental results in the diverse fields of biological research. Frequent publication (36 issues per year) ensures a steady stream of information. The development of the "Breakthroughs and Views" section brings the minireview format to the journal. In addition, the editors have expanded the journalís scope. Research Areas now include: Biochemistry Cell Biology; Developmental Biology; Immunology; Neurobiology; Biophysics; Molecular Biology; Plant Biology.

Current impact factor: 2.28

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 2.281
2012 Impact Factor 2.406
2011 Impact Factor 2.484
2010 Impact Factor 2.595
2009 Impact Factor 2.548
2008 Impact Factor 2.648
2007 Impact Factor 2.749
2006 Impact Factor 2.855
2005 Impact Factor 3
2004 Impact Factor 2.904
2003 Impact Factor 2.836
2002 Impact Factor 2.935
2001 Impact Factor 2.946
2000 Impact Factor 3.055
1999 Impact Factor 3.161
1998 Impact Factor 2.78
1997 Impact Factor 2.671
1996 Impact Factor 2.872
1995 Impact Factor 3.179
1994 Impact Factor 3.4
1993 Impact Factor 3.312
1992 Impact Factor 3.583

Impact factor over time

Impact factor

Additional details

5-year impact 2.50
Cited half-life 8.50
Immediacy index 0.40
Eigenfactor 0.14
Article influence 0.78
Website Biochemical and Biophysical Research Communications website
Other titles Biochemical and biophysical research communications (Online), Biochemical and biophysical research communications
ISSN 1090-2104
OCLC 35247010
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details


  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Pre-print allowed on any website or open access repository
    • Voluntary deposit by author of authors post-print allowed on authors' personal website, or institutions open scholarly website including Institutional Repository, without embargo, where there is not a policy or mandate
    • Deposit due to Funding Body, Institutional and Governmental policy or mandate only allowed where separate agreement between repository and the publisher exists.
    • Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months .
    • Set statement to accompany deposit
    • Published source must be acknowledged
    • Must link to journal home page or articles' DOI
    • Publisher's version/PDF cannot be used
    • Articles in some journals can be made Open Access on payment of additional charge
    • NIH Authors articles will be submitted to PubMed Central after 12 months
    • Publisher last contacted on 18/10/2013
  • Classification
    ​ green

Publications in this journal

  • Cathryn L. Haigh, Simon C. Drew
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    ABSTRACT: The protein misfolding cyclic amplification (PMCA) technique has become a widely-adopted method for amplifying minute amounts of the infectious conformer of the prion protein (PrP). PMCA involves repeated cycles of 20 kHz sonication and incubation, during which the infectious conformer seeds the conversion of normally folded protein by a templating interaction. Recently, it has proved possible to create an infectious PrP conformer without the need for an infectious seed, by including RNA and the phospholipid POPG as essential cofactors during PMCA. The mechanism underpinning this de novo prion formation remains unknown. In this study, we first establish by spin trapping methods that cavitation bubbles formed during PMCA provide a radical-rich environment. Using a substrate preparation comparable to that employed in studies of de novo prion formation, we demonstrate by immuno-spin trapping that PrP- and RNA-centered radicals are generated during sonication, in addition to PrP-RNA cross-links. We further show that serial PMCA produces protease-resistant PrP that is oxidatively modified. We suggest a unique confluence of structural (membrane-mimetic hydrophobic/hydrophilic bubble interface) and chemical (ROS) effects underlie the phenomenon of de novo prion formation by PMCA, and that these effects have meaningful biological counterparts of possible relevance to spontaneous prion formation in vivo.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.048
  • Wonchung Lim, Joonwoo Park, YongHee Lee, Jiyong Hong, YoungJoo Lee
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    ABSTRACT: Subglutinol A is an immunosuppressive α-pyrone diterpenoid isolated from Fusarium subglutinans that exhibits osteogenic activity. Several non-steroid mycotoxins isolated from various strains of Fusarium fungi exhibit female steroid hormone activities. In this study, we characterized the estrogenic activity of subglutinol A (1). Subglutinol A blocked the 17β-estradiol-induced activation of reporter plasmids and endogenous estrogen-responsive target genes in a dose-dependent manner and efficiently destabilized ER proteins as shown using the estrogen receptor antagonist ICI 182,780. Subglutinol A also displaced the specific binding of [3H]17β-estradiol from ER in MCF-7 whole-cell ligand binding assays. These data demonstrate the potential of subglutinol A as an ER antagonist though its competition with 17β-estradiol for direct ER association.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.053
  • Masaya Kato, Ryuta Muromoto, Sumihito Togi, Masashi Iwakami, Yuichi Kitai, Kon Shigeyuki, Kenji Oritani, Dr. Tadashi Matsuda
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    ABSTRACT: The promyelocytic leukemia protein PML acts as a tumor suppressor by forming transcription-regulatory complexes with a variety of repressor proteins. In the present study, we found that endogenous PML suppresses interleukin (IL)-6-induced gene expression as well as phosphorylation and transcriptional activation of STAT3 in hepatoma cells. We also found that PML-mediated suppression of IL-6-induced STAT3 activation by disrupting interactions between STAT3 and HDAC3. These results indicate that PML modulates IL-6-induced STAT3 activation and hepatoma cell growth by interacting with HDAC3.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.040
  • Tong Chen, Yu-wei Wu, Hui Lu, Yuan Guo, Zhi-hui Tang
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    ABSTRACT: Human adipose-derived stem cells (hASCs) are multipotent progenitor cells with multi-lineage differentiation potential including osteogenesis and adipogenesis. While significant progress has been made in understanding the transcriptional control of hASC fate, little is known about how hASC differentiation is regulated by the autocrine loop. The most abundant adipocytokine secreted by adipocytes, adiponectin (APN) plays a pivotal role in glucose metabolism and energy homeostasis. Growing evidence suggests a positive association between APN and bone formation yet little is known regarding the direct effects of APN on hASC osteogenesis. Therefore, this study was designed to investigate the varied osteogenic effects and regulatory mechanisms of APN in the osteogenic commitment of hASCs. We found that APN enhanced the expression of osteoblast-related genes in hASCs, such as osteocalcin, alkaline phosphatase, and runt-related transcription factor-2 (Runx2, also known as CBFa1), in a dose- and time-dependent manner. This was further confirmed by the higher expression levels of alkaline phosphatase and increased formation of mineralization nodules, along with the absence of inhibition of cell proliferation. Importantly, APN at 1 μg/ml was the optimal concentration, resulting in maximum deposition of calcium nodules, and was significant superior to bone morphogenetic protein 2. Mechanistically, we found for the first time that APN increased nuclear translocation of the leucine zipper motif (APPL)-1 as well as AMP-activated protein kinase (AMPK) phosphorylation, which were reversed by pretreatment with APPL1 siRNA. Our results indicate that APN promotes the osteogenic differentiation of hASCs by activating APPL1-AMPK signaling, suggesting that manipulation of APN is a novel therapeutic target for controlling hASC fate.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.03.168
  • Wataru Shoji, Yusuke Suenaga, Yoshiki Kaneko, Rafiqul Islam, Jennifer Alagu, Sana Yokoi, Masaki Nio, Akira Nakagawara
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    ABSTRACT: NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas.
    Biochemical and Biophysical Research Communications 04/2015; 13. DOI:10.1016/j.bbrc.2015.04.050
  • Hiroko Chiue, Tatsuko Yamazoye, Sueo Matsumura
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    ABSTRACT: Previous studies have shown that fibrous collagen undergoes intermolecular cross-linking at multiple sites of the elongated triple-helical regions among adjacent juxtaposed collagen molecules on incubation with a very high concentration of reducing sugar such as 200 mM ribose, and the similarity of the changes in its physicochemical properties to that of senescent collagen aged in vivo has been emphasized. In the present study, however, it was found that when incubated with less than 30 mM ribose, fibrous collagen underwent intermolecular cross-linking primarily between the telopeptide region of a collagen molecule and the triple-helical region of another adjacent collagen molecule, and intermolecular cross-linking between the triple-helical regions of adjacent collagen molecules was very small. Physiological significance of the previous studies thus needs to be reevaluated.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.011
  • Manabu Murakami, Takashi Suzuki, Tsai-Wen Wu, Kenji Kuwasako, Eiki Takahashi, Hiroyuki Watanabe, Agnieszka M. Murakami, Ichiro Miyoshi, Teruyuki Yanagisawa, Hironobu Sasano, Kyoichi Ono, Takayoshi Ohba
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    ABSTRACT: Genetic analyses have revealed an important association between P/Q-type calcium channel activities and hereditary neurological disorders. The P/Q-type channels are composed principally of heterologous multimeric subunits including CaV2.1 and CaVβ4. Of these, the β4 subunit is thought to play a significant role in channel physiology, because a mouse line mutant in that subunit (the lethargic mouse: lh) exhibits a severe ataxic phenotype. The aim of the present study was to elucidate the physiological importance of the β4 subunit.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.03.112
  • Shunsuke Tamaru, Yosuke Mizuno, Hideno Tochigi, Takeshi Kajihara, Yasushi Okazaki, Ryugo Okagaki, Yoshimasa Kamei, Osamu Ishihara, Atsuo Itakura
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    ABSTRACT: The expression of numerous microRNAs (miRNAs) in the trophoblasts changes under low oxygen conditions. However, little is known regarding the regulation of the trophoblast invasion by miRNAs under low oxygen conditions. The aim of this study was to identify those miRNAs and their target genes associated with the trophoblast invasion under low oxygen conditions. Culturing the extravillous trophoblast (EVT) cell line, HTR-8/SVneo, at 2% oxygen as compared to 20% oxygen suppressed trophoblast invasion that correlated with increased expression of microRNA-135b (miR-135b) and decreased expression of the its predicted target gene CXCL12. Overexpression of miR-135b suppressed CXCL12 mRNA expression and invasion of HTR-8/SVneo cells. Adding a neutralizing antibody against CXCL12 to the culture medium suppressed HTR-8/SVneo cell invasion. Reporter assays showed that the 3′UTR sequence of CXCL12 was directly targeted by miR-135b. Our results suggest that miR-135b and CXCL12 play important roles in modulating the EVT invasion under low oxygen conditions.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.055
  • Shoin Tei, Hiroshige T. Ishii, Hiroaki Mitsuhashi, Shoichi Ishiura
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    ABSTRACT: CHRNA1 encodes the α subunit of nicotinic acetylcholine receptors (nAChRs) and is expressed at the neuromuscular junction. Moreover, it is one of the causative genes of Congenital Myasthenic Syndromes (CMS). CHRNA1 undergoes alternative splicing to produce two splice variants: P3A(-), without exon P3A, and P3A(+), with the exon P3A. Only P3A(-) forms functional nAChR. Aberrant alternative splicing caused by intronic or exonic point mutations in patients leads to an extraordinary increase in P3A(+) and a concomitant decrease in P3A(-). Consequently this resulted in a shortage of functional receptors. Aiming to restore the imbalance between the two splice products, antisense oligonucleotides (AONs) were employed to induce exon P3A skipping. Three AON sequences were designed to sterically block the putative binding sequences for splicing factors necessary for exon recognition. Herein, we show that AON complementary to the 5’ splice site of the exon was the most effective at exon skipping of the minigene with causative mutations, as well as endogenous wild-type CHRNA1. We conclude that single administration of the AON against the 5’ splice site is a promising therapeutic approach for patients based on the dose-dependent effect of the AON and the additive effect of combined AONs. This conclusion is favorable to patients with inherited diseases of uncertain etiology that arise from aberrant splicing leading to a subsequent loss of functional translation products because our findings encourage the option of AON treatment as a therapeutic for these prospectively identified diseases.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.035
  • Yong-Soon Park, Swarnalee Dutta, Mina Ann, Jos M. Raaijmakers, Kyungseok Park
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    ABSTRACT: Volatile organic compounds (VOCs) from plant growth-promoting rhizobacteria (PGPR) play key roles in modulating plant growth and induced systemic resistance (ISR) to pathogens. Despite their significance, the physiological functions of the specific VOCs produced by Pseudomonas fluorescens SS101 (Pf.SS101) have not been precisely elucidated. The effects of Pf.SS101 and its VOCs on augmentation of plant growth promotion were investigated in vitro and in planta. Significant growth promotion was observed in plants exposed Pf.SS101 under both conditions, suggesting its VOCs play a key role in promoting plant growth. Solid-phase micro-extraction (SPME) and a gas chromatography-mass spectrophotometer (GC-MS) system were used to characterize the VOCs emitted by Pf.SS101 and 11 different compounds were detected in samples inoculated this bacterium, including 13-Tetradecadien-1-ol, 2-butanone and 2-Methyl-n-1-tridecene. Application of these compounds resulted in enhanced plant growth. This study suggests that Pf.SS101 promotes the growth of plants via the release of VOCs including 13-Tetradecadien-1-ol, 2-butanone and 2-Methyl-n-1-tridecene, thus increasing understanding of the role of VOCs in plant-bacterial inter-communication.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.039
  • Xianhui Hou, Xiaogang Niu
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    ABSTRACT: The inflammasome is a key component of the innate immune system providing the initial defense against invading organisms. Failure of inflammasome formation is the main reason for many innate and acquired immune diseases. Cytosolic protein absent in melanoma 2 (AIM2) has been reported to play an essential role in double-stranded DNA (dsDNA) sensing and inflammasome formation in response to viruses or bacteria infection. The N-terminal pyrin domain (PYD) of AIM2 interacts with the ASC PYD domain, and then recruits downstream proteins to assemble the AIM2 inflammasome. The molecular mechanisms of PYD mediated signaling remain elusive as limited structural information on PYD family. Herein, we characterized the solution structure of mouse AIM2 PYD domain by NMR spectroscopy, and compared it with the crystal structures of its two human homologues. The comparison shows mAIM2 PYD adopts a unique α2-α3 helix conformation distinct from its human homologues, but similar to the pyrin domain of human NLRP10/PYNOD, which belongs to another family. In addition, the aggregation of mAIM2 PYD domain, with the increased salt concentration, reveals that both the charge surface and hydrophobic interaction play important roles in the self-association of mAIM2 PYD.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.046
  • Prakash C. Joshi, Krishna Dubey, Michael F. Aldersley, Meaghen Sausville
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    ABSTRACT: Catalysis by montmorillonites clay minerals is regarded as a feasible mechanism for the abiotic production and polymerization of key biomolecules on early Earth. We have investigated a montmorillonite-catalyzed reaction of the 5´-phosphorimidazolide of nucleosides as a model to probe prebiotic synthesis of RNA-type oligomers. Here we show that this model is specific for the generation of RNA oligomers despite deoxy-mononucleotides adsorbing equally well onto the montmorillonite catalytic surfaces. Optimum catalytic activity was observed over a range of pH (6-9) and salinity (1±0.2M NaCl). When the weathering steps of early Earth that generated catalytic montmorillonite were modified to meet Martian soil conditions, the catalytic activity remained intact without altering the surface layer charge. Additionally, the formation of oligomers up to tetramer was detected using as little as 0.1 mg of Na+-montmorillonite, suggesting that the catalytic activity of a Martian clay return sample can be investigated with sub-milligram scale samples.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.044
  • Elida Adalgisa Neri, Camila Nogueira Alves Bezerra, Gabriella Duarte Queiroz-Leite, Juliano Zequini Polidoro, Nancy Amaral Rebouças
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    ABSTRACT: The main transport mechanism of reabsorption of sodium bicarbonate and fluid in the renal proximal tubules involves Na+/H+ exchanger 3 (NHE3), which is acutely and chronically downregulated by parathyroid hormone (PTH). Although PTH is known to exert an inhibitory effect on NHE3 expression and transcription, the molecular mechanisms involved remain unclear. Here, we demonstrated that, in opossum kidney proximal tubule (OKP) cells, PTH-induced inhibition of Nhe3 gene promoter occurs even in the core promoter that controls expression of the reporter gene. We found that inhibition of the protein kinase A (PKA) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathways transformed PTH from an inhibitor of promoter activity into an activator of that same activity, as did point mutations in the EGR1, Sp1, and Sp3 binding consensus elements in the promoter. In nuclear extracts of PTH-treated OKP cells, we also observed increased expression of EGR1 mRNA and of some Sp3 isoforms. Electrophoretic mobility shift assay showed a supershift of the −61 to −42-bp probe with an anti-EGR1 antibody in PTH-treated cells, suggesting that EGR1 binding is relevant for the inhibitory activity of PTH. We conclude that PTH-induced inhibition of NHE3 transcription is related to higher EGR1 expression; to EGR1 binding to the proximal and core promoters; and to PKA and JAK/STAT pathway activation. This mechanism might be responsible, at least in part, for lower NHE3 expression and sodium reabsorption in renal proximal tubules in the presence of high PTH levels.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.049
  • Rei Narikawa, Keiji Fushimi, Ni-Ni Win, Masahiko Ikeuchi
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    ABSTRACT: Cyanobacteriochromes (CBCRs) are diverse photoreceptors that are found only from cyanobacteria and cover wide range of light qualities. CBCRs are divided into two types regarding the chromophore species they contain: phycocyanobilin (PCB) and phycoviolobilin. Red/green-type CBCRs are widely distributed subfamily among the PCB-binding CBCRs and photoconvert between a red-absorbing thermostable form and a green-absorbing metastable form. Our recent study discovered that a red/green-type CBCR, AM1_1557g2, from a cyanobacterium Acaryochloris marina covalently binds not only PCB but also biliverdin (BV). BV-binding AM1_1557g2 photoconverts between a far-red absorbing form and an orange-absorbing form. We report, herein, that another red/green-type CBCR, AM1_1870g3, from the cyanobacterium A. marina also bound both PCB and BV. PCB- and BV-binding ones showed red/green and far-red/orange reversible photoconversions, respectively. Unexpectedly, absorbing wavelengths are 10-20 nm red-shifted compared with those of AM1_1557g2. These red-shifted characteristics may be useful for optogenetic light switches that work in various organisms.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.045
  • Eun Hyuk Jang, Seong Ah Park, Young Min Chi, Ki Seog Lee
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    ABSTRACT: Succinic semialdehyde dehydrogenases (SSADHs) are ubiquitous enzymes that catalyze the oxidation of succinic semialdehyde (SSA) to succinic acid in the presence of NAD(P)+, and play an important role in the cellular mechanisms including the detoxification of accumulated SSA or the survival in conditions of limited nutrients. Here, we report the inhibitory properties and two crystal structures of SSADH from Streptococcus pyogenes (SpSSADH) in a binary (ES) complex with SSA as the substrate and a ternary (ESS) complex with the substrate SSA and the inhibitory SSA, at 2.4 Å resolution for both structures. Analysis of the kinetic inhibitory parameters revealed significant substrate inhibition in the presence of NADP+ at concentrations of SSA higher than 0.02 mM, which exhibited complete uncompetitive substrate inhibition with the inhibition constant (Ki) value of 0.10 ± 0.02 mM. In ES-complex of SpSSADH, the SSA showed a tightly bound bent form nearby the catalytic residues, which may be caused by reduction of the cavity volume for substrate binding, compared with other SSADHs. Moreover, structural comparison of ESS-complex with a binary complex with NADP+ of SpSSADH indicated that the substrate inhibition was induced by the binding of inhibitory SSA in the cofactor-binding site, instead of NADP+. Our results provide first structure-based molecular insights into the substrate inhibition mechanism of SpSSADH as the Gram-positive bacterial SSADH.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.047
  • Sangho Lee, Minjung Kim, Wonchung Lim, Taeyoung Kim, Chounghun Kang
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    ABSTRACT: Strenuous exercise is known to cause excessive ROS generation and inflammation. However, the mechanisms responsible for the regulation of mitochondrial integrity in the senescent muscle during high-intensity exercise (HE) are not well studied. Here, we show that HE suppresses up-regulation of mitochondrial function despite increase in mitochondrial copy number, following excessive ROS production, proinflammatory cytokines and NFκB activation. Moreover, HE in the old group resulted in the decreasing of both fusion (Mfn2) and fission (Drp1) proteins that may contribute to alteration of mitochondrial morphology. This study suggests that strenuous exercise does not reverse age-related mitochondrial damage and dysfunction by the increased ROS and inflammation.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.038
  • Na Rae Han, Hyun Lee, Song Baek, Jung Im Yun, Kyu Hyun Park, Seung Tae Lee
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    ABSTRACT: Although episomal vectors are commonly transported into cells by electroporation, a number of electroporation-derived problems have led to the search for alternative transfection protocols, such as the use of transfection reagents, which are inexpensive and easy to handle. Polyplex-mediated transport of episomal vectors into the cytoplasm has been conducted successfully in immortalized cell lines, but no report exists of successful transfection of primary cells using this method. Accordingly, we sought to optimize the conditions for polyplex-mediated transfection for effective delivery of episomal vectors into the cytoplasm of primary mouse embryonic fibroblasts. Episomal vectors were complexed with the commercially available transfection reagents Lipofectamine 2000, FuGEND HD and jetPEI. The ratio of transfection reagent to episomal vectors was varied, and the subsequent transfection efficiency and cytotoxicity of the complexes were analyzed using flow cytometry and trypan blue exclusion assay, respectively. No cytotoxicity and the highest transfection yield were observed when the ratio of transfection reagent to episomal vector was 4 (v/wt) in the cases of Lipofectamine 2000 and FuGENE HD, and 2 in the case of jetPEI. Of the three transfection reagents tested, jetPEI showed the highest transfection efficiency without any cytotoxicity. Thus, we confirmed that the transfection reagent jetPEI could be used to effectively deliver episomal vectors into primary cells without electroporation.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.037
  • Michael I. Koukourakis, Dimitra Kalamida, Achilleas Mitrakas, Stamatia Pouliliou, Sofia Kalamida, Efthimios Sivridis, Alexandra Giatromanolaki
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    ABSTRACT: Radiotherapy is an equivalent alternative or complement to radical prostatectomy, with high therapeutic efficacy. High risk patients, however, experience high relapse rates, so that research on radio-sensitization is the most evident route to improve curability of this common disease.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.014
  • Xiangbo Wang, Lu Zhang, Nianhua Ding, Xinghui Yang, Jin Zhang, Jiang He, Zhi Li, Lun-Quan Sun
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    ABSTRACT: Epigenetic inactivation of genes plays a critical role in many important human diseases, especially in cancer. A core mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA, which is catalyzed by DNA methyltransferases (DNMTs). The inhibition of DNMTs may lead to demethylation and expression of the silenced tumor suppressor genes. Although DNMT inhibitors are currently being developed as potential anticancer agents, only limited success is achieved due to substantial toxicity. Here, we utilized a multiplex selection system to generate efficient RNA-cleaving DNAzymes targeting DNMT1. The lead molecule from the selection was shown to possess efficient kinetic profiles and high efficiency in inhibiting the enzyme activity. Transfection of the DNAzyme caused significant down-regulation of DNMT1 expression and reactivation of p16 gene, resulting in reduced cell proliferation of bladder cancers. This study provides an alternative for targeting DNMTs for potential cancer therapy.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.033
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    ABSTRACT: Chronic inflammation is one of the remarkable etiologic factors for various human ailments including cancer. The well known hypothesis is that persistent inflammation in colon can increase the risk of colorectal cancer (CRC). In this study, a pharmacological evaluation of carvacrol, a phenolic monoterpene constituent of essential oils produced from aromatic plant Oreganum vulgarea sp. on colitis associated colon cancer (CACC) induced by 1,2 Dimethylhydrazine (DMH) and dextran sodium sulphate (DSS) in male Fischer 344 rat model was studied. F344 rats were given three subcutaneous injection of DMH (40 mg/kg body wt) in the first week and were given free access to drinking water containing 1% DSS for next one week followed by 7-14 days of water as three cycles. Carvacrol was administrated before and after tumor induction at a concentration of 50mg/kg body weight (o. p). Carvacrol treated groups promotes the endogenous antioxidant system and suppress the inflammation in DMH/DSS induced animals. An increased antioxidant status and restoration of histological lesions in the inflamed colonic mucosa was observed in carvacrol treated rats. This effect was confirmed biochemically by reducing free-radical accumulation and suppressing expression of pro-inflammatory mediators. In this study, Carvacrol significantly increased the anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) glutathione (GSH) levels and reduced lipid peroxides (LPO), myeloperoxidase (MPO) and nitric oxide (NO) as compared to DMH/DSS induced rats. These dramatic changes facilitate the suppression of pro-inflammatory mediators such as inducible nitric oxide synthase (iNOS), and interleukin-1 beta (IL-1β) in CACC induced rats. Taken together, these findings suggest that Carvacrol may play a beneficial role in DMH/DSS induced experimental rat model and serve as an excellent dietary antioxidant as well as anti-inflammatory agent. It may represent novel therapeutic interventions against colon cancer triggered by chronic inflammation. Copyright © 2015. Published by Elsevier Inc.
    Biochemical and Biophysical Research Communications 04/2015; DOI:10.1016/j.bbrc.2015.04.030