Cell Biochemistry and Biophysics (CELL BIOCHEM BIOPHYS )

Publisher: Springer Verlag

Description

Cell Biochemistry and Biophysics is a laboratory-oriented journal devoted to illuminating the biophysical mechanisms controlling cell functions. Formerly Cell Biophysics, the journal publishes state-of-the-art basic, applied, and clinical research in all areas of cellular biochemistry, biophysics,and physiology, but favors studies focusing on the mechanistic aspects.

Impact factor 2.38

  • Hide impact factor history
     
    Impact factor
  • 5-year impact
    2.70
  • Cited half-life
    5.60
  • Immediacy index
    0.45
  • Eigenfactor
    0.01
  • Article influence
    0.92
  • Website
    Cell Biochemistry and Biophysics website
  • Other titles
    Cell biochemistry and biophysics
  • ISSN
    1085-9195
  • OCLC
    33449553
  • Material type
    Periodical, Internet resource
  • Document type
    Journal / Magazine / Newspaper, Internet Resource

Publisher details

Springer Verlag

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Author's pre-print on pre-print servers such as arXiv.org
    • Author's post-print on author's personal website immediately
    • Author's post-print on any open access repository after 12 months after publication
    • Publisher's version/PDF cannot be used
    • Published source must be acknowledged
    • Must link to publisher version
    • Set phrase to accompany link to published version (see policy)
    • Articles in some journals can be made Open Access on payment of additional charge
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The effects of extremely high frequency electromagnetic irradiation and antibiotics on Escherichia coli can create new opportunities for applications in different areas-medicine, agriculture, and food industry. Previously was shown that irradiated bacterial sensitivity against antibiotics was changed. In this work, it was presented the results that irradiation of antibiotics and then adding into growth medium was more effective compared with non-irradiated antibiotics bactericidal action. The selected antibiotics (tetracycline, kanamycin, chloramphenicol, and ceftriaxone) were from different groups. Antibiotics irradiation was performed with low intensity 53 GHz frequency during 1 h. The E. coli growth properties-lag-phase duration and specific growth rate-were markedly changed. Enhanced bacterial sensitivity to irradiated antibiotics is similar to the effects of antibiotics of higher concentrations.
    Cell Biochemistry and Biophysics 01/2015; 71(1):419-424.
  • [Show abstract] [Hide abstract]
    ABSTRACT: In order to elucidate the role of epigenetic alterations in the development of cutaneous squamous cell carcinoma (SCC), we analyzed both gene-specific promoter hypermethylation and repetitive sequence hypomethylation in cutaneous SCC as well as normal skin tissue samples. We showed that methylation of DAPK1 and CDH13 was associated with cutaneous SCC. While methylation frequency of DAPK1 was increased from sun-protected normal skin, sun-exposed normal skin, perilesional to lesional tissues, methylation of CDH13 was almost exclusively detected in cutaneous SCC tissues. Further, methylation of DAPK1 and CDH13 was neither correlated with the presence of HPV nor with the presence of p53 mutations in lesional skin tissues. Finally, we detected trend of reduced methylation level of repetitive sequences from sun-protected, sun-exposed normal skin samples to perilesional, and lesional tissues from SCC patients. We conclude that both gene-specific hypermethylation and repetitive sequence hypomethylation are present in cutaneous SCC tissue samples; these epigenetic changes might represent an independent pathway in the development of cutaneous SCC.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to compare the analgesic efficacy of the ultrasound-guided block of femoral nerve or fascia iliaca compartment in patients who underwent patella fracture surgery. Fifty patients were blinded and randomized into groups treated with continuous fascia iliaca compartment block (CFICB) (n = 25) or continuous femoral nerve block (CFNB) (n = 25) after patella fracture surgery. Analgesic effects of the two methods were assessed and compared. Patients from the two groups showed no significant difference in visible analog scales at rest and during movement, fentanyl consumption, nausea, and vomiting. The time of catheter insertion was significantly shorter in carrying out CFICB compared to that in performing CFNB (8.3 ± 1.4 vs 14.5 ± 3.0 min). Three of the 25 patients in CFNB group experienced dysesthesia of anterior of the thigh, a complication which was not observed in CFICB-treated patients. CFICB and CFNB were equally effective in relieving pain after the patella fracture surgery. However, compared to CFNB, CFICB was found to be safer and easier to perform.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cyclooxygenase (COX), also known as prostaglandin endoperoxide synthase, catalyzes the conversion of arachidonic acid to prostanoids. There are two different isoforms of COX, referred to as COX-1 and COX-2. Overexpression of COX-2 has been demonstrated in various neoplasms. In this study, we plan to utilize COX-2 in understanding the difference of squamous cell carcinoma and keratoacanthoma which have many similarities in both morphological and histological features. The objective of this study is to study the expression of COX-2 in squamous cell carcinoma and keratoacanthoma and to discuss its clinical significance. The expression of COX-2 in 55 cases of skin tumors (including 30 specimens of squamous cell carcinoma, 25 specimens of keratoacanthoma) and 20 normal skin tissues was detected by immunohistochemical technique. The positive expression of COX-2 was found in 73.3 % (22/30) of squamous cell carcinoma and 12 % (3/25) of keratoacanthoma cases. The positive expression rate of COX-2 in 55 skin tumors (45.5 %) was significantly higher than that in normal skin tissues (5 %) (χ 2 = 10.598 %, P COX-2 in squamous cell carcinoma (73.3 %) was significantly higher than that in keratoacanthoma (12 %) (χ 2 = 20.69, P COX-2 overexpression may play a potential role in the pathogenesis of skin tumors. The positive expression rate of COX-2 is associated with the malignant degree of the tumor, and also it may help differentiating squamous cell carcinoma from keratoacanthoma.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The McMurray test is used to evaluate patients with meniscal tears of the knee. Analysis of the sensitivity and specificity of McMurray test and the correlation between McMurray sign and postoperative outcomes are helpful to screen the candidates for arthroscopic meniscectomy. The present study compares the results of McMurray test with arthroscopic examination in patients with knee osteoarthritis. We studied 149 patients diagnosed as meniscal lesion with osteoarthritis by radiology and had arthroscopic surgery. Our data show that positive McMurray sign implies good postoperative outcomes for the patients with meniscal tear associated with osteoarthritis. For patients with osteoarthritis, those whose positive McMurray signs are in line with their radiological findings indicate they are eligible candidates for arthroscopic meniscectomy. Our study suggests that McMurray sign can be used as an indication for both symptomatic meniscal tear and postoperative outcomes.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular block and treated with calcium channel blockers were retrospectively assessed. The patients had varying degrees of atrioventricular block (confirmed by Holter ECG) and myocardial ischemia-like ST–T changes. Two patients were II type I AVB, two patients II type II AVB, and the remaining one patient was III AVB. All patients were all diagnosed with right coronary artery spasm by coronary angiography. The patients were treated with calcium channel blockers. No patient reported recurrence of chest pain or chest discomfort. On Holter ECG monitoring, no significant myocardial ischemia or atrioventricular block was seen. In conclusion, calcium channel blockers are effective and safe in the treatment of atrioventricular block caused by coronary spasm.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The objective of the study is to evaluate the clinical significance of screening for fetal spina bifida aperta by ultrasound examination and intracranial translucency (IT) measurement at 11–13+6 weeks of gestation. About 1,479 women at 11–13+6 weeks of gestation in our hospital in 2012 were included as observation group, and 1,608 women at 11–13+6 weeks of gestation without IT measurement in 2011 was included as controls. Detection rates of fetal spina bifida aperta in two groups were compared. The translucency thickness between the brain stem and choroid plexus and crown-rump length (CRL) in mid-sagittal view of the fetal face was measured, and translucency thickness and CRL in fetuses with spina bifida and healthy ones were compared. Detection rate of fetal spina bifida aperta in observation group was significantly higher than that in control group (six cases in observation group and one case in control group, p = 0.046). IT thickness was significantly lower in fetuses with spina bifida aperta (0.01 ± 1.25 mm) than that in healthy ones (1.73 ± 0.32 mm) (p r = 0.702, p r = 0.001, p = 0.081). Ultrasound examination with IT measurement at 11–13+6 weeks of gestation can be used to screen for fetal spina bifida aperta, and the reduction of IT thickness is an indicator of spina bifida aperta.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Mesenchymal stem cells (MSCs) have been shown to be able to inhibit cancer cells growth. In this study, we investigate the role and the molecular mechanism of hypoxia-inducible factor 1-alpha (HIF-1α) in inhibition of cancer cell proliferation by human MSCs through depletion and overexpression of HIF-1α in human MSCs. We found that the cell culture medium from HIF-1α-depleted Z3 cells significantly promotes breast cancer MCF-7 cell proliferation and colony formation. The expression of p21 is increased in MCF-7 cells, but p53 level remains unchanged. In contrast, the cultured medium from HIF-1α-overexpressed Z3 cells dramatically inhibits MCF-7 cell proliferation and colony formation. The expression of p21 is inhibited in MCF-7 cells, but p53 does not change. We conclude HIF-1α promotes inhibitory effect of human MCSs on breast cancer cell proliferation and colony formation. This process is tightly correlated with cell cycle protein p21 level in cancer cells.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this study is to compare the effectiveness of olanzapine combined with ondansetron or ondansetron alone in preventing chemotherapy-induced nausea and vomiting (CINV) of non-small cell lung cancer (NSCLC). A total of 84 NSCLC patients were equally randomized into intervention group and control group. Both groups were intravenously administered with ondansetron 8 mg 30 min before chemotherapy. In the intervention group, olanzapin 10 mg was orally administered for 8 days, beginning from the first morning of chemotherapy. The antiemetic effectiveness was evaluated in the first chemotherapy cycle. The incidence of acute vomiting was 33.33 % (14/42) and 54.76 % (23/42) in the intervention group and control group (p p NSCLC patients, particularly for the delayed type.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Sorcin, a soluble resistance-related calcium-binding protein, belongs to the small penta-EF-hand family. Recent study reported that upregulation of sorcin correlated with metastasis and poor prognosis of colorectal cancer (CRC). In the present study, we explored the regulatory role of sorcin in CRC metastasis. To investigate the role of sorcin in CRC metastasis, sorcin overexpressed with empty vector as control in CRC cell line (HCT116). The effect of sorcin overexpression on cell migration and invasion was evaluated via wound healing and transwell assay, respectively. Sorcin-induced changes in EMT process were evaluated by estern blot. Furthermore, the role of PI3K/Akt in the regulatory effect of sorcin on cell migration and invasion, and EMT process was explored by suppressing Akt activity in sorcin-overexpressed HCT116 cells. Sorcin overexpression in HCT116 cells resulted in a significant increase in cell migration and invasion. Sorcin overexpression also markedly promoted the EMT process. More importantly, our results revealed that sorcin stimulated EMT process through activating PI3K/Akt signaling. In summary, this study indicated that the promoting effect of sorcin on CRC metastasis was, at least in part, through PI3K/Akt signaling. The findings in this study highlight the effectiveness and therapeutic potential to utilize sorcin-targeted strategies in the treatment of CRC.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study describes clinical experience and curative effect of radiophotographically controlled nasointestinal intubation in the treatment of small intestine arrangement in recurrent postoperative adhesive ileus. A 300-cm-long nasointestinal tube was installed into the jejunum through the nasal cavity under radiophotographic observation in 25 patients with recurrent postoperative adhesive ileus. The tube was advanced into the ileum by enterokinesia to relieve adhesive ileus and conduct small bowel arrangement. Duration of tube installation was 13 ± 11 min (range of 9–36 min). The success rate was 88 %. In 3 patients, assistance by a gastroscope was required. Twenty-one out of 25 patients were cured, making the cure rate of 84 %. The time for intestinal arrangement was 18.5 ± 3 days. The patients were followed up for 6 months to 2 years; there was 1 case of recurrence, while other patients recovered smoothly with no recurrence. A non-surgical nasointestinal intubation under radiophotography is a simple, safe, and effective management with few complications and beneficial outcome in recurrent postoperative adhesive ileus.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Although aggressive therapeutic regimen has been applied in the treatment of Glioblastoma (GBM), the prognosis of patients with GBM remains poor. Preclinical studies have demonstrated the efficacy of Suntinib in GBM both in vitro and in vivo. In this study, we showed that the cytotoxicity was enhanced by transfection with miR-145 mimic. In addition, we suggested that the enhanced cytotoxicity of Sunitinib by miR-145 mimic was mediated by inhibition of both P-gp and Bcrp.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Neuroendocrine tumors are composed by a heterogeneous group of tumors with a wide range of morphologic, functional, and behavioral characteristics. These tumors are generally slow growing and behave in an indolent fashion. However, they have the potential to spread, especially for poorly differentiated neuroendocrine carcinomas, and when they do, they can be very aggressive, with high propensity for distant metastases, and difficult to treat with current modalities. As poorly differentiated neuroendocrine carcinomas rarely occur in extrapulmonary sites, to the best of our knowledge, this is the first case report of a poorly differentiated duodenal neuroendocrine carcinoma with diffuse liver and bone metastases as the primary manifestation. Despite receiving a trial of chemotherapeutic regimen, the patient had progressive intrahepatic cholestasis and died of subacute hepatic failure.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: To explore the clinical effect of internal fixation treatment of intra-articular calcaneal fractures with titanium plate, we used open reduction and internal fixation with titanium plate to 48 treated feet from 42 patients with intra-articular calcaneal fractures. The efficacy of surgical treatment was evaluated based on assessment of pain, function, and line of force aspects according to the American Orthopedic Foot and Ankle Society scoring system. Our data show that internal fixation with titanium plate is an effective treatment for calcaneal fractures. It provides satisfactory reduction, reliable fixation, and early rehabilitation.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Osteosarcoma is the most common type of solid bone cancer, which is the second leading cause of cancer-related death. Hypoxia is an ordinary phenomenon in solid tumor tissues and can induce cell apoptosis but the specific molecular mechanism remains unclear. In this study, we explored the effect and the molecular mechanism of Transglutaminase 2 (TG2) on cell apoptosis in osteosarcoma U2OS cells under hypoxia. We found the enzymatic activity of TG2 is significantly increased and the expression of TG2 is remarkably up-regulated under hypoxia condition. Cell apoptotic rate is markedly increased upon knockdown of TG2 by siRNA under hypoxia. We further investigated the mechanism of cell apoptosis and found Bax protein is significantly increased after depletion of TG2 under hypoxia. Moreover, our data also show that cytochrome C (Cyt C) is significantly increased in cytoplasm and markedly decreased in mitochondria of U2OS cells after depletion of TG2 under hypoxia. Our results suggest that TG2 can inhibit tumor cell apoptosis through down-regulation of Bax and prevention of release Cyt C from mitochondria into cytoplasm.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study was set to explore the role of P2X2 and P2X5 as the important molecules in sensory afferent of bladder in female overactive bladder (OAB) patients with the bladder hyperesthesia. Sixty-eight OAB patients admitted in Southwest Hospital affiliated to the Third Military Medical University during September, 2011–December, 2012 were selected and included in the experimental group (OAB group) and 30 healthy volunteers during the same period were included as the control group. We recorded voiding diary and urodynamic results, and immunohistochemistry analysis was used to detect P2X2 and P2X5 receptor in interstitial cell of Caja (ICC) in bladder tissue of female OAB patients and healthy volunteers, to tentatively explore the effect of P2X2 and P2X5 in bladder hyperesthesia. Urodynamic study has important diagnostic value in the diagnosis and differential diagnosis of OAB. P2X2 receptor was significantly up-regulated in bladder ICC in OAB group. The blockage of P2X2 receptor could significantly inhibit the contraction of bladder muscle strips, decrease the bladder pressure and the electric discharge of pelvic nerve. PET and urodynamic study showed that micturition desire sense in PAG area of pons in OAB patients was significantly increased compared with the control group. The up-regulation of P2X2 in ICC is an important factor to cause bladder hyperesthesia in OAB patients. PET and urodynamic study indicate that the bladder-originated nervous impulses are important cause of OAB. This study provides a basis for the study of P2X2 receptor in ICC in bladder hyperesthesia of OAB patients.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Koumine is an alkaloid separated from traditional Chinese herb Gelsemium elegans. In this study, anticancer activity and underlying mechanisms were investigated with an extract using human breast cancer cells. The survival rate was reduced in a concentration- and time-dependent manner as assessed by MTT assay. After incubation for 48 h, typical apoptotic morphological changes were observed by Hoechst 33258 dye assay. Flow cytometry result revealed that the treatment obviously induced G2/M arrest and apoptosis in MCF-7 cells. Furthermore, Western blotting demonstrated the down-regulation of protein expression of Bcl-2, whereas Bax and caspase-3 expressions were up-regulated. Therefore, we propose that koumine has the potential to be a future breast cancer chemotherapeutic agent.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hepatocellular carcinoma (HCC) is one of the most aggressive tumors in humans. The typical therapeutic strategies include a combination of chemotherapy, radiotherapy, and surgery, whereas the survival rate of patients is very poor. Ginsenoside Rh2 has been reported to have therapeutic effects on some tumors, but its effect on HCC has not been extensively evaluated. Here, we show that ginsenoside Rh2 can effectively inhibit the proliferation and cell survival of HCC cells in vitro and in a mouse model. Moreover, the inhibition of the tumor growth appears to result from combined effects on decreased tumor cell proliferation and cell viability. Further analyses suggest that ginsenoside Rh2 may have its anti-tumor effect through inhibition of epidermal growth factor receptor (EGFR) signaling pathway. Recombinant EGFR was given together with ginsenoside Rh2 to the tumor cells, which completely blocked the anti-tumor effect of ginsenoside Rh2. Our data also show that miR-491 is up-regulated in SMMC-7721 cells after Rh2 treatment. There is a negative correlation between EGFR and miR-491 levels in SMMC-7721 cells and miR-491 directly targeted EGFR at translational level. Our data not only reveal an anti-tumor effect of ginsenoside Rh2 but also demonstrate that this effect may function via activation and inhibition of EGFR signaling in HCC cells. The results suggest miR-491 can be a promising regulatory factor in EGFR signal transduction.
    Cell Biochemistry and Biophysics 01/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Detection of 2,4,6-trinitrotoluene (TNT) has been extensively studied since it is a common explosive filling for landmines, posing significant threats to the environment and human safety. The rapid advances in synthetic biology give new hope to detect such toxic and hazardous compounds in a more sensitive and safe way. Biosensor construction anticipates finding sensing elements able to detect TNT. As TNT can induce some physiological responses in E. coli, it may be useful to define the sensing elements from E. coli to detect TNT. An E. coli MG1655 genomic promoter library containing nearly 5,400 elements was constructed. Five elements, yadG, yqgC, aspC, recE, and topA, displayed high sensing specificity to TNT and its indicator compounds 1,3-DNB and 2,4-DNT. Based on this, a whole cell biosensor was constructed using E. coli, in which green fluorescent protein was positioned downstream of the five sensing elements via genetic fusion. The threshold value, detection time, EC200 value, and other aspects of five sensing elements were determined and the minimum responding concentration to TNT was 4.75 mg/L. According to the synthetic biology, the five sensing elements enriched the reservoir of TNT-sensing elements, and provided a more applicable toolkit to be applied in genetic routes and live systems of biosensors in future.
    Cell Biochemistry and Biophysics 01/2015;