Journal of Liquid Chromatography &amp Related Technologies (J LIQ CHROMATOGR R T )

Publisher: Taylor & Francis

Description

The Journal of Liquid Chromatography & Related Technologies publishes an outstanding selection of critical, peer-reviewed papers dealing with analytical, preparative and process-scale liquid chromatography of all types and related technologies such as TLC; capillary electrophoresis; supercritical fluid extraction and chromatography; membrane separation technology; field-flow techniques; and others. Journal of Liquid Chromatography & Related Technologies also publishes special topical issues devoted to specific technologies and applications. Book reviews, software reviews, and a calendar of meetings, symposia, and expositions are also included.

  • Impact factor
    0.57
    Show impact factor history
     
    Impact factor
  • 5-year impact
    0.67
  • Cited half-life
    9.20
  • Immediacy index
    0.09
  • Eigenfactor
    0.00
  • Article influence
    0.15
  • Website
    Journal of Liquid Chromatography & Related Technologies website
  • Other titles
    Journal of liquid chromatography & related technologies, Journal of liquid chromatography and related technologies
  • ISSN
    1082-6076
  • OCLC
    32501604
  • Material type
    Periodical, Internet resource
  • Document type
    Journal / Magazine / Newspaper, Internet Resource

Publisher details

Taylor & Francis

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Restrictions
    • 12 month embargo for STM, Behavioural Science and Public Health Journals
    • 18 month embargo for SSH journals
  • Conditions
    • Some individual journals may have policies prohibiting pre-print archiving
    • Pre-print on authors own website, Institutional or Subject Repository
    • Post-print on authors own website, Institutional or Subject Repository
    • Publisher's version/PDF cannot be used
    • On a non-profit server
    • Published source must be acknowledged
    • Must link to publisher version
    • Set statements to accompany deposits (see policy)
    • Publisher will deposit to PMC on behalf of NIH authors.
    • STM: Science, Technology and Medicine
    • SSH: Social Science and Humanities
    • 'Taylor & Francis (Psychology Press)' is an imprint of 'Taylor & Francis'
  • Classification
    ​ yellow

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple, rapid, and reproducible method for the qualification of RADIX PAEONIAE ALBA raw material and SHENSHAO composite injection was developed. The fingerprint was established using HPLC techniques. Chromatographic fingerprint analysis was carried out on a ODS analytical column. Acetonitrile-phosphoric acid solution (pH 3.0) in gradient program was used as the mobile phase. The detecting wavelength was 230 nm for RADIX PAEONIAE ALBA and 210 nm for SHENSHAO composite injection, respectively. The standard fingerprint chromatograms and the similarity of 10 batches of samples were also established. It was demonstrated that the HPLC fingerprint was a powerful tool to characterize the quality of traditional Chinese medicines.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: A high performance liquid chromatography (HPLC) method was developed and validated for simultaneous quantification of nine major components including volatile and nonvolatile ingredients in POGOSTEMON CABLIN (Blanco) Benth. The analysis was performed on a Cosmosil 5 C18-AR-II column (4.6 mm − 250 mm, 5 mm) with 0.2% formic acid in water–acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R2 > 0.9991) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) ere less than 36.32 and 122.09 ng. The relative standard deviations (RSDs) for intra-day and inter-day repeatability were no more than 3.23% and 4.85%, respectively. The sample was stable for at least 48 hr. The spike recoveries of nine components were 96–106%. The established method was successfully applied to determine nine components in thirteen samples from different locations. The results showed that the newly developed HPLC-DAD method was sensitive, precise, and accurate and could be used for quality control of P. cablin.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: According to previous reports, the concentration of urinary proline (Pro), hydroxyproline (Hyp), and N-ethylglycine (Neg) varies in association with various diseases, especially, Neg as a tumor marker was recently found in association with bone metastasis cancers. We developed a low-cost, highly sensitive precolumn high–performance liquid chromatography (HPLC) method for simultaneous determination of Pro, Hyp, and Neg in urine of patients with cancers, bone metastasis cancers, and bone metastasis cancers that have appeared after radiotherapy and chemotherapy treatment. The analytes in the urine were labeled with 4–methoxybenzenesulfonyl fluoride (MOBS–F) at 40°C. The derivatives were separated on a reversed-phase column by gradient elution and monitored with ultraviolet (UV) detection at 232 nm. The detection limits for Pro, Hyp, and Neg were 6.0 pmol/injection, 4.0 pmol/injection, and 50 pmol/injection (S/N = 3), respectively. To some extent, Neg levels in their urine show there were relevance to their health conditions or therapy progress. We believe that the developed method may be a promising measure for providing a useful reference to diagnose the illness and monitor the results for bone metastasis cancers after radiotherapy and chemotherapy treatment.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: Next to water, tea is one of the most consumed beverages all around the world and fruit teas provide a dietary source of biologically active compounds. Many functional foodstuff of fruit origin, including red fruit tea, have entered the markets providing new developments in the domain of quality control and food security scientific research. For this reason, the goal of our study was to establish a method for authentication of 12 commercially available fruit teas (raspberry, strawberry, blueberry, rose hip, wild berry) using HPTLC fingerprinting. The HPTLC fingerprints of red fruit and tea extracts obtained in visible light and in fluorescence at 366 nm after the derivatization with Natural Product/Polyethylene glycol (NP/PEG) reagents indicated different characteristic colored zones allowing a clear differentiation between all teas, in terms of their type and producer. Also, a good correlation between the data from chromatograms and type of teas and the producers, respectively, was obtained.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: In this work the degradation of furfural in aqueous solution was investigated by means of photo catalysis of TiO2 nanoparticles with 25 nm crystallite sizes and surface area of 50 ± 10 m2 g−1, that was immobilized on a glass support by impregnation and calcination methods. The effects of amount of TiO2, irradiation intensity, and pH on efficiency of TiO2 nanoparticles were studied by HPLC-UV method. The optimum conditions are found as follows: pH = 7, 120 min of irradiation, and 125 W light intensity. In addition, the mechanism of photocatalytic degradation of furfural was studied by LC-Mass technique. According to the LC-MASS data we proposed three steps for furfural degradation including aldehyde group oxidation, photo-oxidation of aromatic ring and ring cleavage. Furfural photocatalytic reaction in 140min led to production of maleic and 2, 3-Dihydroxy-succinic (tartaric) acids as intermediates.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: We herein developed an ultra-performance liquid chromatography coupled with an electrospray ionization quadruple time-of-flight tandem mass spectrometry (UPLC/ESI-QTOF-MS) for the first time to characterize 12 iridoid glycosides from FRUCTUS LIGUSTRI LUCIDI (FLL), a commonly used traditional Chinese medicinal herb for the treatment of kidney-deficiency and osteoporosis. In this study, a rapid and effective method was established to analyze iridoid glycosides, and 12 iridoid glycosides were detected and identified by high accurate MS data using Masslynx software. The results reveal that the iridoid glycosides were subjected to deglycosylation, major fragmentation, McLafferty rearrangement and ring cleavage, successively. Salidroside was firstly found in FLL. We have demonstrated UPLC/ESI-QTOF-MS is feasible to rapidly and reliably characterize the iridoid glycosides in FLL.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: A simple gradient reversed-phase ultra-performance liquid chromatographic (RP-UPLC) method for the simultaneous determination of five pesticides, namely Monocrotophos, Thiram, Carbendazim, Carbaryl, and Imidacloprid has been developed. The aforementioned method involves sample preparation by QuEChERS method and quantification by ultra-performance liquid chromatography with the tunable dual wavelength detector. The mobile phase composition was varied to improve peak resolution and peak sensitivity. Choosing the match between the stationary phase and mobile phase composition, the developed RP-UPLC method not only can simplify the procedure but also significantly reduce the analysis time. The method presents an average recovery of more than 87.0% and 96.0%, in repeatability and intermediate precision conditions, respectively, with adequate precision (RSD from 0.8 to 20.5%), for all compounds. The method was applied to determine these pesticide residues in Mango and pomegranate samples.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: Chromatographic properties and chemical stability of 12 kinds of phenolic compounds Z. schinifolium were investigated and analyzed to use as an antioxidant substance in dentifrices. Twelve compounds were completely separated under 0.01% phosphoric acid-acetonitrile gradient condition at 20°C and detected by photodiode array detector. Each compound showed good linearity at optimized wavelength, and showed good precision and accuracy in dentifrices. Using this analytical method, phenolic compounds in Z. schinifolium fraction and dentifrices were successfully determined. Caffeic acid, quercetin, and xanthoxylin decomposed in aqueous solution, and other compounds were preserved over 94%. It was better to store phenolic compounds under weak acidic conditions (Z. schinifolium -containing dentifrice was manufactured and analyzed. In Z. schinifolium dentifrices, 44% of phenolic compounds remained after 2 mo.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014; 37(12).
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    ABSTRACT: Quantitative structure-retention relationships (QSRR) were developed for eleven 1,6-anhydrohexose and D-aldopentose derivatives using their retention data (RM0 values), obtained by normal phase (NP) thin-layer chromatography (TLC) and partition coefficients for n-octanol/water bi-phase system – logP. In order to select molecular descriptors that best describe the retention behavior of the investigated molecules, principal component analysis (PCA) was carried out, followed by hierarchical cluster analysis (HCA) and multiple linear regression (MLR). The best QSRR models were validated by leave-one-out technique and by the calculation of statistical parameters. Established mathematical models are meaningful and statistically significant and can successfully predict retention behavior of investigated 1,6-anhydrohexose and D-aldopentose derivatives.
    Journal of Liquid Chromatography &amp Related Technologies 07/2014;
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    ABSTRACT: The lipophilicity parameters of fourteen new antiproliferative 8-10-substituted 6H-quinobenzothiazines were determined theoretically using 9 computational methods and experimentally by reversed-phase thin-layer chromatography on the RP-18 silica plates with acetone-aqueous TRIS buffer as the mobile phase. The experimental R M values were linearly dependent on the concentration of acetone in the mobile phase and extrapolated to 0% of acetone that produced the lipophilicity parameter R M0. The parameter R M0 and specific hydrophobic surface area b were significantly intercorrelated showing congeneric classes of quinobenzothiazines. The parameter R M0 was correlated with molecular descriptors (molar mass, molar volume, and molar refractivity), predicted and tested biological activities, and was further transformed into parameter log PTLC by use of the calibration curve. The theoretical log P calcd values differed depending on the calculating programs and were compared with experimental log PTLCvalues showing different prediction powers of calculated programs. Tetracyclic 6H-quinobenzothiazine was as lipophilic as tricyclic 10H-phenothiazine. The determined parameters are discussed in the terms of the structure-lipophilicity relationships.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: Capillary electrophoresis (CE) has been recognized as a highly attractive separation technique for determining agrochemical residues in agricultural produces and environmental matrices due to its extremely high column efficiency, rapid analysis, and less reagent consumption. However, a small sample size and a short optical path length make the low concentration samples detected with an ultraviolet (UV) detector difficult or even impossible without sample preconcentration. The on-line concentrating techniques promise to extremely increase sample size without the changes of CE instrument and make the detection of agrochemical residues possible by CE. This review describes these on-line preconcentration techniques and comments their applications in the determination of agrochemical residues.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: N-butyl 3,5-di-(O-p-chlorobenzoyl)-D-2-deoxy-ribofuranoside is a very important medical intermediate, which is commonly constituted by two diastereomers (α- and β-anomers). In this paper the HPLC determination condition was studied for the first time. The Hypersil - SiO2(4.6 × 250 mm, 5 µm) column was used and the mobile phase was a mixture of hexane- isopropanol (98:2). The wavelength of determination was 240 nm. N-butyl 3,5-di-(O-p-chlorobenzoyl)-D-2-deoxy-ribofuranoside's diastereomers have been separated thoroughly with the HPLC determination condition. This method is accurate, simple, rapid, and suitable for laboratory and industry.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: In order to demonstrate physicochemical comparability of a recombinant protein medicament as a drug substance (Active Pharmaceutical Ingredient or API), such as Rituximab, one must first choose suitable orthogonal analytical methods that selectively provide information regarding the identity and heterogeneity of the molecule. The application of these methods in the investigation of process-derived variability of the reference molecule can yield valuable information to establish comparability criteria. This could become the basis to determine the critical physicochemical attributes that should be verified by relevant in-process controls and batch release criteria. In this paper we show the determination and analysis of relevant attributes from two different proposed biosimilars. We show differences in Reditux proposed Rituximab biosimilar, mainly in charge heterogeneity patterns, whereas no major differences were found for Kikuzubam as related to the Mabthera reference batches. The detected differences are an indication of the capability of several analytical methods to show relevant physicochemical attributes during comparability exercises.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: Using an amino column (Supelcosil LC-NH2) and different mixtures of acetonitrile-water, quantitative structure-retention relationship models are discussed. These models are based on computed molecular descriptors representing numerically structured features of some saccharides. The obtained results are underlining the lipophilicity/hydrophilicity balance, and how this is controlling the separation of the saccharides. The resulting models ensued are characterized by a highly predictive capacity evaluated through a series of representative statistical indices (R2, Q2, SDEC, SDEP, s, and F). Moreover, using genetic algorithms for selection of the most important descriptors in the model, one-, two-, and three-descriptor models were selected and the contribution of each one was explained. As the majority of the selected descriptors is highly related to the functional groups existing in the molecule and to the effects caused by their presence, the retention mechanism involved in the chromatographic process appears to be mainly controlled by polar interactions.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: A new chromatographic and densitometric method has been established for quantitative analysis of lumefantrine and artemether present together in combined tablet to treat malaria. The method employed TLC aluminum plate precoated with silica gel 60 F254 as the stationary phase. The solvent system consisted of toluene:ethyl acetate:acetic acid (2:7.5:0.5, v/v/v) as the mobile phase. Densitometric analysis was carried out in the reflectance mode at 269 nm for lumefantrine and 519 nm for artemether. The method is specific for analyte constituents examined and characterized by high sensitivity. The LOD and LOQ of lumefantrine were 7.32 and 26.15 ng; for artemether, 2.43 and 6.12 ng per band, respectively. Recovery from 98.40% to 99.45% for lumefantrine and 100.09% to 101.61% for artemether and linear range from 60 to 360 ng for lumefantrine and 10 to 60 ng for artemether per band. The method was characterized by good precision with RSD from 0.11 to 1.41.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: Triapine is a novel inhibitor of ribonucleotide reductase (RR). It can remove ferric ion from the catalytic di-iron-tyrosyl radical center of RR, inhibit de novo synthesis of deoxyribonucleotides, and impair the required ratio of DNA to cell mass during cell division and DNA repair. Recent studies indicate that triapine is particularly useful for enhancing the radiation-mediated cytotoxicity in cervical and colon cancers; hence, the interest of further clinical study of this compound is high. Due to its chelating property, quantitative measurement of triapine is an analytical challenge, which is affected by multi-factors that influence the complexation reaction between triapine and ferric ion. In this work, the effects of pH, buffer composition, and EDTA, as well as other metal ions on the spectral absorptivity and chromatographic retention of triapine have been investigated. The optimized conditions for analytical measurement of triapine have been applied to the development of an LC-UV method for quantitation of triapine, in which hydrazinecarbothioamide is used as an internal standard. Both triapine and the internal standard can be separated on a Waters Xterra RP18 column (2.1 × 150 mm, 5 µm particle size) by a mobile phase of 18.0% acetonitrile and 82.0% ammonium bicarbonate-EDTA buffer [i.e., 10.0 mM (NH4)HCO3 and 5.00 mM EDTA (v/v) at pH 8.5]. The detection of triapine and the internal standard is carried out by UV detector at wavelength of 360 nm. This method has a linear calibration range from 3.00 to 1.00 × 103 ng mL−1 with inter-assay precision (%CV) and accuracy (%RE) of 2–4% and −3–9%, respectively. This work examines the underlying factors affecting quantitative determination of triapine and provides an accurate and reliable method for quantitation of triapine.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: HPLC isolation and spectroscopic characterization studies, along with the examination of DPPH radical scavenging capacity, were performed for identifying the major coumarin constituents of the bark extract of Aesculus hippocastanum L. collected from Turkey. The isolation process was carried out using a semi-preparative RP-HPLC with diode array detector. The major phenolic components of bark extract were identified by means of analytic RP-HPLC. Isolated compounds were characterized when necessary, along with comparison of available spectroscopic database, by UV-vis, 1H, 1³C NMR, FTIR, and HR-MS techniques. Radical scavenging capacity was determined by spectrophotometric DPPH assay. The isolated compounds A and B, in accordance with the RP-HPLC elution profile, were identified as 6-(beta-D-glucopyranosyloxy)-7-hydroxy-2H-1-benzopyran-2-one and 6,7-dihydroxycoumarin, whereas the third isolate compound C was characterized as 7,8-dihydroxy-6-methoxycoumarin. Compounds A and B exhibited important spectroscopic features with the emission maxima of 515 nm and 445 nm, respectively. The crude extract from A. hippocastanum bark revealed a remarkably high DPPH radical scavenging capacity with the EC50 value of 11.94 ± 0.41 µg/mL. The isolated components A, B, and C also exhibited scavenging capacities with EC50 values of 260.9 ± 3.3, 4.83 ± 0.04, and 494.8 ± 16.4 (in µg/mL), in the order of respective RP- HPLC profile.
    Journal of Liquid Chromatography &amp Related Technologies 06/2014; 37(10).
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    ABSTRACT: This article focuses on the determination of cyanotoxin microcystin-LR using techniques such as solid-phase extraction (SPE) and HPLC-DAD for the detection and quantification of microcystin-LR in cyanobacterial blooms, which are frequently present in fee-fishing ponds and fish farms. Tests performed with SPE showed that this procedure was not necessary for all samples because there were cases in which no changes were obtained at the interfering peaks near the retention time of the studied analyte. However, because composition of such samples is very complex and varies greatly, the SPE must be promoted, although this process requires a lengthier analysis time frame and an increase in cost. The method was validated in accordance with the Brazilian National Health Surveillance Agency (ANVISA) and FDA. The limit of detection was set at 0.1 igmL ∼2 and the limit of quantification at 0.5 figmL-1. The analytical curve obtained, y = 21.849 x + 1.5888, with r = 0.9950, showed good linearity. Microcystin-LR was detected in more than half of the cyanobacterial blooms samples analyzed, being also quantified in two of them. This procedure showed the importance of analyzing microcystin-LR in cyanobacterial blooms in order to evaluate water quality and safety offish consumption.
    Journal of Liquid Chromatography &amp Related Technologies 05/2014; 37(9).
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    ABSTRACT: A simple method for the measurement of testosterone in male saliva samples using liquid chromatography coupled with a triple-quadrupole mass spectrometer was developed here. Testosterone and its d-3-labeled internal standard (IS) were extracted from 0.5 mL of the saliva sample after pretreatment with acetonitrile (ACN) + 1% formic acid (FA). After centrifugation, the supernatant was diluted and underwent a dispersive liquid–liquid microextraction (DLLME) treatment. The resulting sedimented phase was then dried under a gentle steam of nitrogen gas followed by a reconstitution step before liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis. Testosterone and its IS were ionized via electrospray in the positive ionization mode. Testosterone and the IS [M + H]+ parent ions (m/z 289.2 and 292.2, respectively) and the same product ions (m/z 97.1) were used for the ion transitions in the selected reaction monitoring (SRM) mode. The calibration curve was linear over the concentration range of 0.15 to 5.0 nM. Intra-day accuracy was in the range of 82% to 107%. Intra- and inter-day precision varied in the range of 1.9% to 8.7% and 7.0% to 17.5%, respectively. Finally, testosterone in male saliva samples taken from four healthy adult volunteers was successfully measured and quantified.
    Journal of Liquid Chromatography &amp Related Technologies 05/2014; 37(9).

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