Journal of AOAC International (J AOAC INT)

Publisher: AOAC International, AOAC International

Journal description

The Journal of AOAC International publishes fully refereed contributed papers in the fields of chemical and biological analysis: on original research on new techniques and applications, collaborative studies, authentic data of composition, studies leading to method development, meeting symposia, newly adopted AOAC approved methods and invited reviews.

Current impact factor: 1.39

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 1.385
2012 Impact Factor 1.233
2011 Impact Factor 1.199
2010 Impact Factor 1.229
2009 Impact Factor 1.216
2008 Impact Factor 1.122
2007 Impact Factor 1.549
2006 Impact Factor 1.352
2005 Impact Factor 1.046
2004 Impact Factor 1.147
2003 Impact Factor 1.037
2002 Impact Factor 0.907
2001 Impact Factor 1.33
2000 Impact Factor 1.066
1999 Impact Factor 0.95
1998 Impact Factor 0.948
1997 Impact Factor 1.138
1996 Impact Factor 1.256
1995 Impact Factor 0.797
1994 Impact Factor 0.762

Impact factor over time

Impact factor

Additional details

5-year impact 1.32
Cited half-life 8.10
Immediacy index 0.19
Eigenfactor 0.01
Article influence 0.30
Website Journal of AOAC (Association of Official Analytical Chemists) International website
Other titles Journal of AOAC International
ISSN 1060-3271
OCLC 24929715
Material type Periodical, Internet resource
Document type Journal / Magazine / Newspaper, Internet Resource

Publisher details

AOAC International

  • Pre-print
    • Author cannot archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Conditions
    • The publisher will deposit in PubMed Central on behalf of NIH authors
  • Classification
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Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The applicability of direct bioautography, the combination of planar chromatography with antimicrobial assay, is demonstrated with special emphasis on its recent developments such as BioArena and the use of genetically modified luminescent bacteria. Its methodological advancement is put into a historical perspective. In comparison with other commonly used antimicrobial susceptibility tests, the main advantage of direct bioautography resides in its simplicity, rapidity, and ability to detect separated individual matrix components exhibiting antimicrobial activity in situ. It is confirmed with examples that high-throughput direct bioautography is suitable as a biomonitoring-screening system for bioassay-guided isolation.
    Journal of AOAC International 07/2015; 98(4):850-6. DOI:10.5740/jaoacint.SGE1-Moricz
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    ABSTRACT: Antioxidant properties of selected herbs were investigated using electron paramagnetic resonance (EPR) spectroscopy. This was possible by measuring changes in the intensity of the EPR spectrum that resulted from the interaction of the stable radical 1,1-diphenyl-2-picrylhydrazyl with antioxidants found in herbal samples. Moreover, the total phenolic content (TPC) was measured using UV-Vis spectroscopy. The values of trolox equivalent antioxidant capacity were in the range of 10.95 to 505.95 μmol Trolox/1 g of dry weight of sample. TPC values were in the range of 3.38 to 63.13 mg of gallic acid/1 g of dry weight. The results showed that all of the investigated herbs exhibit antioxidant properties. A positive and significant correlation between antioxidant activity and total phenolic content was observed. The studied herbs could be a good source of natural antioxidants.
    Journal of AOAC International 07/2015; 98(4):862-5. DOI:10.5740/jaoacint.SGE3-Bartoszek
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    ABSTRACT: The 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes combines isothermal amplification and bioluminescence to detect Listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. The 3M MDA Listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook 8.09 (2011) Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products and Environmental Samples for deli turkey, and the AOAC Official Method of Analysis(SM) 993.12 Listeria monocytogenes in Milk and Dairy Products for full-fat (4% milk fat) cottage cheese following the current AOAC guidelines. A total of 16 laboratories located in the continental United States and Canada participated in this collaborative study. For deli turkey, 125 g test portions were evaluated using heat-stressed cells by each method. For full-fat cottage cheese, 25 g test portions were evaluated using nonheat-stressed cells. Each matrix had three inoculation levels: an uninoculated control level (0 CFU/test portion), and two levels artificially contaminated with L. monocytogenes, a low inoculum level (0.2-2 CFU/test portion) and a high inoculum level (2-5 CFU/test portion). In total, 1584 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD) model. Results obtained for the low inoculum level full-fat cottage cheese test portions produced a difference in cross-laboratory POD (dLPOD) value of -0.08 with a 95% confidence interval (CI) of (-0.20, 0.05). For the low-level deli turkey test portions, a dLPOD value of -0.02 with a 95% CI of (-0.14, 0.11) was obtained.
    Journal of AOAC International 07/2015; 98(4):980-92. DOI:10.5740/jaoacint.15-031
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    ABSTRACT: Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA(®) Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol-water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.
    Journal of AOAC International 07/2015; 98(4):939-45. DOI:10.5740/jaoacint.14-211
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    ABSTRACT: A comparison was made of measurements of neutral detergent fiber concentrations obtained with AOAC Method 2002.04 and modified methods using pressurized environments or direct use of industrial heat-stable α-amylase in samples of forage (n = 37), concentrate (n = 30), and ruminant feces (n = 39). The following method modifications were tested: AOAC Method 2002.04 with replacement of the reflux apparatus with an autoclave or Ankom(220®) extractor and F57 filter bags, and AOAC Method 2002.04 with replacement of the standardization procedures for α-amylase by a single addition of industrial α-amylase [250 μL of Termamyl 2X 240 Kilo Novo Units (KNU)-T/g] prior to heating the neutral detergent solution. For the feces and forage samples, the results obtained with the modified methods with an autoclave or modification of α-amylase use were similar to those obtained using AOAC Method 2002.04, but the use of the Ankom(220) extractor resulted in overestimated values. For the concentrate samples, the modified methods using an autoclave or Ankom(220) extractor resulted in positive systematic errors. However, the method using industrial α-amylase resulted in systematic error and slope bias despite that the obtained values were close to those obtained with AOAC Method 2002.04.
    Journal of AOAC International 07/2015; 98(4):883-9. DOI:10.5740/jaoacint.14-156
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    ABSTRACT: Pomegranate fruit (Punica granatum L.) is a source of numerous phenolic compounds, and it contains flavonoids such as anthocyanins, anthocyanidins, cyanidins, catechins and other complexes of flavonoids, ellagitannins, and hydrolyzed tannins. Pomegranate juice shows antioxidant, antiproliferative, and anti-atherosclerotic properties. The antioxidant capacity (TEAC) of the pomegranate juices was measured using electron paramagnetic resonance (EPR) spectroscopy and 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)) as a source of free radicals, and the total phenolic (TP) content was measured using UV-Vis spectroscopy. All the examined pomegranate juices exhibited relatively high antioxidant properties. The TEAC values determined by means of EPR spectroscopy using Trolox (TE) as a free radical scavenger were in the range of 463.12 to 1911.91 μmol TE/100 mL juice. The TP content measured by the Folin-Ciocalteu method, using gallic acid (GA) as a free radical scavenger, widely varied in the investigated pomegranate juice samples and ranged from 1673.62 to 5263.87 mg GA/1 L juice. The strongest antioxidant properties were observed with the fresh pomegranate juices obtained from the fruits originating from Israel, Lebanon, and Azerbaijan. Correlation analysis of numerical data obtained by means of EPR spectroscopy (TEAC) and UV-Vis spectroscopy (TP) gave correlation coefficient (r) = 0.90 and determination coefficient (r(2)) = 0.81 (P <0.05).
    Journal of AOAC International 07/2015; 98(4):866-70. DOI:10.5740/jaoacint.SGE4-Kozik
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    ABSTRACT: A simple, sensitive, and rapid HPLC method was developed to analyze bakuchiol and two furocoumarins (psoralen and angelicin) simultaneously in bakuchiol extracts from Psoralea corylifolia seeds. The analysis was performed within 30 min on a phenyl-hexyl column using gradient elution with a mobile phase composed of water and methanol with UV detection at 260 nm for bakuchiol and 246 nm for psoralen and angelicin. The method was validated with respect to linearity (r(2) > 0.99 for all components), accuracy (>95% for all components), and precision (<2% RSD for both interday and intraday). Sensitivity of impurity detection in the sample was achieved as low as 0.36 and 0.31 μg/mL for psoralen and angelicin, respectively. Therefore, the method is suitable for QC of P. corylifolia extracts and bakuchiol related samples.
    Journal of AOAC International 07/2015; 98(4):902-6. DOI:10.5740/jaoacint.14-228
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    ABSTRACT: An LC/MS method has been developed for the simultaneous quantification of five flavonoids, i.e., mosloflavone, negletein, gardenin B, 5-methoxy-6,7-methylenedioxyflavone, and 5,6,7-trimethoxyflavone, in different ultrasound assisted solvent extracts of Actinocarya tibetica. The chromatographic separation was achieved on a Chromolith Speed ROD RP-18e column with gradient elution using methanol and 0.1% formic acid in water. The calibration curves of all five analytes showed good linearity (R(2) > 0.991). Accuracy and precision were within the required limits. The developed method could serve as an effective method for QC of A. tibetica. The investigated compounds were determined simultaneously for the first time in A. tibetica or any other plant.
    Journal of AOAC International 07/2015; 98(4):907-12. DOI:10.5740/jaoacint.14-270
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    ABSTRACT: The method detection limit (MDL, 99% chance of detecting a positive result in a single replicate), as per the United States Code of Federal Regulations, was determined for a protocol using an ultrafiltration based automated waterborne pathogen concentration device. Bacillus anthracis Sterne strain spores were seeded at low levels into 100 L reagent water samples. Suspect colonies were confirmed through morphological, chemical, and genetic tests. Samples of 100 L (n = 14) of reagent water were seeded with five B. anthracis CFUs each. To confirm the estimated detection limit, a second set (n = 19) of 100 L reagent water samples were seeded at a higher level (7 CFUs). The second estimate of the MDL could not be pooled with the first, due to significant difference in variance. A third trial (n = 7) seeded with 10 CFUs produced an estimate of the MDL that could be pooled with the higher previous estimate. Another trial consisting of eight 100 L samples of tap water were seeded with approximately 7 CFUs. Recovery in these samples was not significantly different from the pooled MDL. Theoretically a concentration of 4.6 spores/100 L would be required for detection 95% of the time, based on a Poisson distribution. The calculated pooled MDL, based on experimental data was approximately 6 B. anthracis CFU/100 L (95% confidence interval 4.8 to 8.4). Detection at this level was achieved in municipal water samples.
    Journal of AOAC International 07/2015; 98(4):1003-12. DOI:10.5740/jaoacint.12-461
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    ABSTRACT: A rapid, simple, and sensitive dispersive liquid-liquid microextraction procedure followed by HPLC-UV was applied to determine the benzoate and sorbate in foods. The method was optimized for some variables including extraction solvent type and volume, dispersing solvent type and volume, and the effects of salt and pH. Optimum conditions were determined as follows: sample volume, 5 mL; extraction solvent (chloroform) volume, 250 μL; disperser solvent (acetone) volume, 1.2 mL; NaCl amount, 0.75 g/5 mL at pH 4. Sixty samples were analyzed, including 15 doogh, 15 fruit juice, 15 cookie, and 15 tomato paste; benzoic acid was detected in 57 samples (95%) at levels up to 448.1 μg/mL and sorbic acid in 31 samples (51.6%) at levels up to 1369 μg/mL. Under the optimum experimental conditions, the LOD and LOQ were determined as 0.1 and 0.5 μg/mL for benzoate and 0.08 and 0.3 μg/mL for sorbate, respectively. The results showed that these preservatives are commonly used at high levels in yogurt drinks (dooghs) and cookies. Also, the concentration of benzoic acid that was detected in the tomato paste and fruit juice samples was low but may affect children and sensitive persons.
    Journal of AOAC International 07/2015; 98(4):962-70. DOI:10.5740/jaoacint.14-260
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    ABSTRACT: Two novel spectrophotometric determination procedures based on retention of Allura Red onto Amberlite XAD-1180 and XAD-16 resins for its preconcentration, purification, and separation were developed. Analytical parameters of the methods including pH, eluent type, sample volume, and sample and eluent flow rates, were investigated and optimized. Interference effects of some cations, anions, and widely used food dyes were also investigated. Detection limits of the two methods were found to be 1.2 and 1.5 μg/L for XAD-1180 and XAD-16 columns, respectively, under optimum conditions. Linear calibration curve ranges of the methods were 0.4-8.0 and 0.5-6.0 μg/mL of Allura Red for XAD-1180 and XAD-16 resins, respectively. Preconcentration factors were found as 80 for both the XAD-1180 and XAD-16 columns using maximum sample volume and minimum eluent volume. RSDs of the methods were below 6% throughout all experiments. All absorbance measurements were performed at 506 nm. Validations of the methods were performed comparatively with determination of the Allura Red contents of some foodstuff, pharmaceutical, and energy drink samples. Allura Red concentrations in investigated solid and liquid samples ranged from 298 to 501 μg/g and 53.8 to 508 μg/mL, respectively. Satisfactory results were obtained from the real samples analysis. Allura Red contents of samples were determined to be highly similar using the two extraction methods. Comparisons of the methods were performed by analysis of Allura Red contents of the real samples. In addition to analytical parameters, adsorption isotherm studies were performed for the two kinds of Amberlite resins. It was observed that developed methods fit the linear form of the Freundlich adsorption isotherm model. All of the experimental results suggested that the developed SPE procedures are suitable for separation, preconcentration, and determination of Allura Red in solid and liquid matrixes.
    Journal of AOAC International 07/2015; 98(4):946-52. DOI:10.5740/jaoacint.14-222
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    ABSTRACT: Aeromonas spp. are opportunistic pathogens causing a broad spectrum of human illnesses like gastroenteritis, chronic diarrhea, wound infections, peritonitis, urinary tract infections, and septicemia. Their ability to grow in foods stored in a refrigerator poses a substantial threat for human consumption. We investigated the prevalence of Aeromonas from commercial food products across Riyadh, Saudi Arabia. A total of 250 samples were randomly collected and processed for the isolation and identification of Aeromonas by morphological and biochemical means and for their identification by PCR. A total of 102 strains of Aeromonas were isolated, including 47% from raw meat samples, 34% from raw fish samples, and 18.6% from milk and dairy products; 56.8% were identified as A. hydrophila and 43.1% as A. sobria. Antibiotic susceptibility tests done revealed 100% sensitivity to chloramphenicol, colistin, ciprofloxacin, and nitrofurantoin. 16S rDNA PCR revealed the presence of the 953 bp fragment in all the strains. The present investigation suggested the occurrences of A. sobria and A. hydrophila in human consumable stored and refrigerated foods.
    Journal of AOAC International 07/2015; 98(4):927-9. DOI:10.5740/jaoacint.14-257
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    ABSTRACT: A single-laboratory validation (SLV) is presented for the simultaneous determination of 10 ultratrace elements (UTEs) including aluminum (Al), arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), mercury (Hg), molybdenum (Mo), lead (Pb), selenium (Se), and tin (Sn) in infant formulas, adult nutritionals, and milk based products by inductively coupled plasma (ICP)/MS after acidic pressure digestion. This robust and routine multielemental method is based on several official methods with modifications of sample preparation using either microwave digestion or high pressure ashing and of analytical conditions using ICP/MS with collision cell technology. This SLV fulfills AOAC method performance criteria in terms of linearity, specificity, sensitivity, precision, and accuracy and fully answers most international regulation limits for trace contaminants and/or recommended nutrient levels established for 10 UTEs in targeted matrixes.
    Journal of AOAC International 07/2015; 98(4):953-61. DOI:10.5740/jaoacint.14-276
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    ABSTRACT: The 5-day sodium carbonate-ammonium nitrate extraction assay (5-day method) has been recognized by the American Association of Plant Food Control Officials as a validated test method to identify fertilizers or beneficial substances that provide plant-available silicon (Si). The test method used the molybdenum blue colorimetric assay to quantify percentage Si; however, laboratories may use inductively coupled plasma optical emission spectroscopy (ICP-OES) for elemental analysis. To examine the use of either colorimetric or ICP-OES methods for Si determination, the 5-day method was performed on the following Si-containing compounds; wollastonite, sand, biochar, and a basic oven furnace (BOF) slag. Grow-out studies using Zinnia elegans were also performed using varying rates of the wollastonite, biochar, and BOF slag. Our results show using the 5-day method, wollastonite had the highest extracted amounts of silicic acid (H4SiO4) at 4% followed by biochar (2%), BOF slag (1%), and sand (0%). Extraction values calculated using either the molybdenum blue colorimetric assay or ICP-OES for detection of the H4SiO4 had a significant correlation, supporting the application of either detection method for this type of analysis. However, when extracted values were compared to amounts of Si taken up by the plants, the 5-day method overestimated both wollastonite and biochar. While this method is a valid indicator test for determining a soluble Si source, other plant species and methods should be perused to potentially provide more quantitative analyses for plant-available Si content of all materials.
    Journal of AOAC International 07/2015; 98(4):890-5. DOI:10.5740/jaoacint.14-205
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    ABSTRACT: Matricaria recutita L. (chamomile) and Achillea millefolium L. (yarrow) are very common herbs growing in meadows, pathways, crop fields, and home gardens. Preparations from these plants, e.g., infusions or alcohol extracts, are widely used as remedies. Both chamomile and yarrow have anti-inflammatory, analgesic, antimicrobial, and antioxidant properties. Most microbiological assays used today give information only on activity of whole extracts and do not provide information on the composition and activity of individual components. This problem can be solved by using TLC with direct microbiological detection, i.e., TLC-direct bioautography (TLC-DB), followed by LC/MS of active fractions. The aim of our study was chemical and microbiological screening of plant components of chamomile and yarrow tinctures using derivatization reagents and TLC-DB against eight bacterial strains: Staphylococcus epidermidis, S. aureus, methicillin-resistant S. aureus, Escherichia coli, Pseudomonas syringae pv. maculicola, Xanthomonas campestis pv. vesicatoria, Aliivibrio fischeri, and Bacillus subtilis. The identity of compounds exhibiting the widest range of activity (apigenin and α-linolenic acid) was confirmed by LC/MS.
    Journal of AOAC International 07/2015; 98(4):857-61. DOI:10.5740/jaoacint.SGE2-Choma