Pakistan Journal of Biological Sciences (Pakistan J Biol Sci )

Publisher: Asian Network for Scientific Information


Pakistan Journal of Biological Sciences is an international official journal of Asian Network for Scientific Information publishing original articles, reviews and short communications of a high scientific and ethical standard in biological sciences. Subjects covered include: animal science, bacteriology, biological chemistry, forestry, genetics and molecular, biology, immunology and microbiology, plant and soil science, pharmacology and toxicology, virology, zoology and fisheries.

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    Pakistan Journal of Biological Sciences website
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    Periodical, Internet resource
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    Journal / Magazine / Newspaper, Internet Resource

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: In order to study caudal fin rot with emphasis on Aeromonas sp. and Pseudomonas sp. in Salmo trutta caspius from the salmonids propagation and breeding center of Shahid Bahonar of kelardasht region, One hundred and eighty brood stocks having fin damage symptoms were chosen. Two bacterial samples from each fish were cultured on Aeromonas and Pseudomonas specific media. To identify isolated bacterial colonies by API20E diagnostic system, samples obtained from bacterial cultures 18 to 24 h were prepared and were inoculated into the cupules of test strips. At the end of incubation, after addition of reagents if they are necessary, the results recorded on the results sheets and were analyzed by Apiwe software. The results of API20E diagnostic systems showed that Aeromonas genus bacteria including Aeromonas hydrophila with 100% frequency while the bacterial genus of Pseudomonas including Pseudomonas fluorescens, Pseudomonas aeruginosa and Pseudomonas putida with 36. 84, 31.57 and 21.05% frequency, respectively. Also 10.25% of Pseudomonas samples were unidentified.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):434-8.
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    ABSTRACT: There is a growing interest in the screening of antihyperlipidemic activity and the present study deals with the screening of a polyherbomineral formulation (AF-LIP) which possess many important ingredients reported to have antihyperlipidemic potency. Acute antihyperlipidemic activity was evaluated by using Triton WR-1339 (100 mg kg(-1)) and chronic, induced by high fat diet. Total Cholesterol (TC), triglycerides (TG), Low Density Lipoproteins (LDL), High Density Lipoproteins (HDL) and Very Low Density Lipoproteins (VLDL) were examined in addition to HMG-CoA reductase enzyme activity and fecal cholesterol excretion. In Triton WR-1339 (acute model) at the dose of 400 mg kg(-1), AF-LIP significantly lowered TC, TG, very Low Density Lipoproteins Cholesterol (VLDL-C), Low Density Lipoproteins Cholesterol (LDL-C) levels with simultaneous increase in High Density Lipoproteins Cholesterol (HDL-C) levels (p < 0.01) at 6 and 24 h. Also there was significant reduction in TC and LDL-C levels at 48 h at the dose of 400 mg kg(-1). In chronic model also at the dose of 100, 200 and 400 mg kg(-1), AF-LIP significantly reduced (p < 0.001) TC and LDL-C levels with increase in HDL-C levels. TG and VLDL-C levels were also not much affected. HMG-CoA reductase enzyme activity when estimated was not much decreased. Also AF-LIP showed significant reduction in atherogenic index (p < 0.01) with significant increase in HDL/TC ratio (p < 0.01). Fecal cholesterol excretion was significantly enhanced (p < 0.01) in all the test doses of AF-LIP. AF-LIP may be beneficial for the treatment of atherosclerosis, since atherosclerosis is one of the secondary complications of hyperlipidemia.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):346-55.
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    ABSTRACT: This study describes the development and validation of dissolution tests for sustained release Dextromethorphan hydrobromide tablets using an HPLC method. Chromatographic separation was achieved on a C18 column utilizing 0.5% triethylamine (pH 7.5) and acetonitrile in the ratio of 50:50. The detection wavelength was 280 nm. The method was validated and response was found to be linear in the drug concentration range of 10-80 microg mL(-1). The suitable conditions were clearly decided after testing sink conditions, dissolution medium and agitation intensity. The most excellent dissolution conditions tested, for the Dextromethorphan hydrobromide was applied to appraise the dissolution profiles. The method was validated and response was found to be linear in the drug concentration range of 10-80 microg mL(-1). The method was established to have sufficient intermediate precision as similar separation was achieved on another instrument handled by different operators. Mean Recovery was 101.82%. Intra precisions for three different concentrations were 1.23, 1.10 0.72 and 1.57, 1.69, 0.95 and inter run precisions were % RSD 0.83, 1.36 and 1.57%, respectively. The method was successfully applied for dissolution study of the developed Dextromethorphan hydrobromide tablets.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):429-33.
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    ABSTRACT: The present study was designed to measure the monthly and seasonal variation in the lipid and carbohydrate content of Schizothorax esocinus. Total lipid content of the fish was measured by Folch's method and the carbohydrate content was evaluated by Dubois method. The lipid content of Schizothorax esocinus varied seasonally, with maxima and minima in a year. The highest lipid content (0.45 g g(-1)) was measured in the July and the lowest lipid content (0.25 g g(-1)) in November. Evaluating the proximate composition of fish is important aspect in fish nutrition. The carbohydrate does not vary so much with a slight increase in spring (0.18%) and a slight decrease in autumn season (0.10%) with an average value of 0.14%. Total protein profile was also carried out in different organs of the fish by using SDS PAGE. The fish is a good source of lipid, carbohydrate and proteins, required for the balanced diet of human consumption.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):447-50.
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    ABSTRACT: Wood Specific Gravity (SG) is a measure of the amount of structural material a tree species allocates to support and strength. In the present study, specific gravity varied among the five different woods at three different sites from 0.40 in Populus nigra at site III (Shopian) to 0.80 in Parrotiopsis jacquemontiana at site II (Surasyar). Among the three different sites, specific gravity varied from 0.73 to 0.80 in Parroptiosis jacquemontiana; in Robinia pseudoacacia it varied from 0.71 to 0.79; in Salix alba, it varied from 0.42 to 0.48; In Populus nigra it varied from 0.40 to 0.48 and in Juglans regia it varied from 0.59 to 0.66. On the basis of the specific gravity variation patterns these woods were categorized as light (Salix alba, Populus nigra) moderately heavy (Juglans regia) and moderately heavy to heavy (Robinia pseudoacacia, Parrotiopsis jacquemontiana) which predicts their properties like strength, dimensional stability with moisture content change, ability to retain paint, fiber yield per unit volume, suitability for making particleboard and related wood composite materials and suitability as a raw material for making paper.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):395-401.
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    ABSTRACT: This study aimed to assess the association of Tumor Necrosis Factor (TNF-alpha) Locus-308 variant, TNF-alpha and Interleukin (IL-10) Cytokine with the risk of silicosis and its progress in Indonesian cement factory workers, There is an urgent need to explore the determining factors other than exposure since silicosis is chronic progressive and life threatening but remains found, though much industrial hygiene effort has been made. This study population was 6,069 workers registered during 31 December 1990 to 31 December 2003. First, prospective study with Nested Case Control design was conducted on 336 workers in 2003, ten years later the progression of silicosis was assessed in 2013. The result showed proportion of the genetic variation on TNF-alpha on Locus -308 in Indonesia was significantly (p = 0.02) higher on silicosis (13.45%) than nonsilicosis (5.45%) but lower than silicosis in Africa and US miners, since susceptibility loci might vary in different ethnic groups. The sign and symptoms remained as simple silicosis after ten years; The TNF-alpha:IL-10 ratio > 1 was a risk factor in silicosis; the ratio of TNF-alpha:IL-10 > 1 caused a rapid decline of lung function compare to ratio < 1, the decline was chronic progressive during ten years yet not causing significant dyspnea among the cases. Further studies with enlarged sample size are needed. The study concluded, the genetic variation on TNF-alpha gene locus -308 was a risk factor of silicosis in Indonesia cement factory. Its role is indirect, but through mechanism of controlling the blood Cytokine level ratio of TNF-alpha toward IL-10.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):419-23.
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    ABSTRACT: Diabetes is the most common endocrine disease and its prevalence is reaching epidemic proportion worldwide. In 2002, WHO Expert Committee on diabetes mellitus recommended an urgent and further evaluation of the folkloric methods of managing the disease. In response to this recommendation, several medicinal plants are currently being investigated for their hypoglycaemic activity and one of such plants is Tamarindus indica. Tamarindus indica is a slow growing tree that is resistant to strong winds and perennial. The stem-bark extract of the plant is used locally for the management of diabetes. The stem-bark extract of Tamarindus indica L. was investigated for its hypoglycemic action on experimentally induced hyperglycaemic Wistar rats using a single dose of alloxan monohydrate (150 mg kg(-1) IP). The oral LD50 of the extract was found to be greater than 5,000 mg kg(-1). Phytochemical screening revealed the presence of carbohydrates, glycosides, saponins, flavonoids, cardiac glycosides, tannins, alkaloids and triterpenes. The 1000 mg kg(-1) dose of the extract lowered the blood glucose level significantly (p < 0.05) at the 4th, 8th and 16th h. The 500 mg kg(-1) lowered the BGL significantly (p < 0.05) throughout the study. In the oral glucose load method the 1000 mg kg(-1) dose of the extract significantly (p < 0.05) lowered elevated blood glucose at the 3rd and 5th. The 500 mg kg(-1) lowered the blood glucose from the 1st to the 5th, while the 250 mg kg(-1) also lowered the blood glucose level but only significantly at the 5th h. The extract is practically non toxic when administered orally. The stem-bark extract of Tamarindus indica Linn significantly lowered elevated Blood Glucose concentration (BGL) in the experimental animal models, while the crude extract was able to prevent an elevation in BGL when used in the oral glucose load model.
    Pakistan Journal of Biological Sciences 02/2014; 17(3):414-8.