Journal of Microbiology and Biotechnology (J Microbiol Biotechnol)

Publisher: Hanʼguk Sanŏp Misaengmul Hakhoe

Journal description

Current impact factor: 1.32

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 1.32
2012 Impact Factor 1.399
2011 Impact Factor 1.381
2010 Impact Factor 1.224
2008 Impact Factor 2.062
2007 Impact Factor 2.062
2006 Impact Factor 2.037
2005 Impact Factor 1.744
2004 Impact Factor 1.663
2003 Impact Factor 1.202
2002 Impact Factor 1.364
2001 Impact Factor 1.338
2000 Impact Factor 1.083
1999 Impact Factor 0.809
1998 Impact Factor 0.436
1997 Impact Factor 0.226

Impact factor over time

Impact factor
Year

Additional details

5-year impact 1.47
Cited half-life 4.60
Immediacy index 0.12
Eigenfactor 0.01
Article influence 0.37
Website Journal of Microbiology and Biotechnology website
ISSN 1017-7825
OCLC 261226927
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Microalgae have been considered as the most promising candidate for future generation of biofuel. UV irradiation and chemicals are two important mutagenic methods for the enhancement of algal biofuel. The effect of ultraviolet-A radiation and EMS on the biomass and fattyacid content and profile of a dominant green alga Chlorella minutissima MCC 5 have been generate the mutants showing high lipid production. Fluroscent staining by for lipid offer a rapid and expensive tool to measure the neutral lipid content of the mutant cell has been screened by Nilered stain. A statically increase of 53% and 46% increase in the lipid productivity was observed in the EMS and UV treated mutated species than the wild species. The mutant shows higher content of stearic acid (C 18:0), arachidic acid (C 24:0), myristic acid (C 14:0), palmitic acid (C 16:0) in UV exposed cells and myristic acid (C 14:0), palmitic acid (C 16:0), oleic acid (C 18: 1) and linolelaidic acid (C 18: 2) in EMS treated cells. Moreover, the yield of oleic acid (C 18:1) was increased in EMS treated mutants. In addition that the present study also shows that the biomass accumulation was suspended faster in mutated species. Thus, UV and EMS mutant may be appropriate method in microalgae breeding and large scale production of biofuel.
    Journal of Microbiology and Biotechnology 05/2015;
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    ABSTRACT: Rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonads were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU-67 and P. putida QAU-90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Some such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the PQQ dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized for their possible role in growth promotion was probed in vitro in lettuce while in vivo in rice, bean and tomato plants. The results showed significant differences (p≤0.05) in parameters such as: plant height, fresh weight and dry weight etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence on the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.
    Journal of Microbiology and Biotechnology 05/2015; 25(5).
  • Journal of Microbiology and Biotechnology 04/2015; 25(4):503-510. DOI:10.4014/jmb.1409.09035
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    ABSTRACT: Recently, we isolated HY253, a novel decahydrofluorene analog, with the molecular structure of 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol from the roots of Aralia continentalis, which is called Dokwhal, the traditional medicinal herb. Also, we reported previously its cytotoxic activity on cancer cell proliferation in human lung cancer A549 and cervical cancer HeLa cells. The current study was aimed to evaluate its detailed molecular mechanisms on cell cycle arrest and apoptotic induction in human hepatocellular carcinoma HepG2 cells. A flow cytometric analysis of HepG2 cells treated with 60 micrometer HY253 revealed appreciable cell cycle arrest at the G1 phase via inhibition of Rb phosphorylation and down-regulation of cyclin D1. Furthermore, using western blots, up-regulation of cyclin-dependent kinase inhibitors, such as p21(CIP1) and p27(KIP1), was associated with this G1 phase arrest. Moreover, TUNEL assay and immunoblottings revealed apoptotic induction in HepG2 cells treated with 100 micrometer HY253 for 24 h, which is associated with cytochrome c release from mitochondria, via down-regulation of anti-apoptotic Bcl-2 protein which in turn resulted in activation of caspase-9 and -3, and proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, we suggest that HY253 may be a potent chemotherapeutic hit compound for treating human liver cancer cells via up-regulation and activation of p53 gene.
    Journal of Microbiology and Biotechnology 02/2015; 25(3). DOI:10.4014/jmb.1501.01069
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    ABSTRACT: Cadaverine (1, 5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, but there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using recombinant Escherichia 35 coli strain that overexpressing the E.coli MG1655 cadA gene and various parameters were 36 investigated for the whole-cell bioconversion of lysine to cadaverine. The high concentration of lysine resulting that the synthesis of pyridoxal-5'-phosphate (PLP) and it was found that a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, 91% of consumption and about 80% conversion of lysine to cadaverine were successfully achieved.
    Journal of Microbiology and Biotechnology 02/2015;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cadaverine (1, 5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, but there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using recombinant Escherichia 35 coli strain that overexpressing the E.coli MG1655 cadA gene and various parameters were 36 investigated for the whole-cell bioconversion of lysine to cadaverine. The high concentration of lysine resulting that the synthesis of pyridoxal-5'-phosphate (PLP) and it was found that a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, 91% of consumption and about 80% conversion of lysine to cadaverine were successfully achieved.
    Journal of Microbiology and Biotechnology 02/2015;
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    ABSTRACT: VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). Percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1 indicating that VvpM-mediated cell death occurs through necroptosis. Appearance of autophagic vesicles and lipidated form of light chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, a multifarious actions of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.
    Journal of Microbiology and Biotechnology 02/2015; 25(2). DOI:10.4014/jmb.1501.01007
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    ABSTRACT: Lactobacillus species have been shown to enhance intestinal epithelial barrier function, modulate host immune responses, and suppress the growth of pathogenic bacteria, yeasts, molds, and viruses. Thus, lactobacilli have been used as probiotics for treating various diseases, including intestinal disorders, and as biological preservatives in the food and agricultural industry. However, the molecular mechanisms used by lactobacilli to suppress pathogenic bacterial infections have been poorly characterized. We previously isolated Lactobacillus plantarum JSA22 from buckwheat sokseongjang, a traditional Korean fermented soybean food, and possessed high enzymatic, fibrinolytic, and broad-spectrum antimicrobial activity against food-borne pathogens. In this study, we investigated the effects of L. plantarum JSA22 on the growth of Salmonella Typhimurium and S. Typhimurium-induced cytotoxicity by stimulating the host immune response in intestinal epithelial cells. The results showed that co-incubation of S. Typhimurium and L. plantarum JSA22 with intestinal epithelial cells suppressed S. Typhimurium infection, S. Typhimurium-induced NF-κB activation, IL-8 production, and lowered phosphorylation of both Akt and p38. These data indicated that L. plantarum JSA22 has probiotic properties, and can inhibit S. Typhimurium infection of intestinal epithelial cells. Our findings can be used to develop therapeutic and prophylactic agents against pathogenic bacteria.
    Journal of Microbiology and Biotechnology 01/2015; 25(4). DOI:10.4014/jmb.1501.01006
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    ABSTRACT: The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase (ADH) of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry, as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35°C, 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using recombinant Corynebacterium glutamicum-based biocatalyst.
    Journal of Microbiology and Biotechnology 01/2015; 25(4). DOI:10.4014/jmb.1501.01001
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    ABSTRACT: In the course of screening for novel cell cycle inhibitors and apoptotic inducers, CR389 elucidated as 5-(1H-benzoimidazol-2-yl)-1H-pyridin-2-one was generated as a new hit compound. Flow cytometric analysis and Western blots of PA-1 cells treated with 40 μΜ of CR389 revealed an appreciable cell cycle arrest at the G2/M phase through direct inhibition of CDK1 complex. In addition, the activation of p53 via phosphorylation at Ser15 and the subsequent up-regulation of p21(CIP1) showed that CR389 also induces p53-dependent-p21(CIP1)-mediated cell cycle arrest. Furthermore, the apoptotic induction in 40 μΜ of CR389-treated PA-1 cells is associated with the release of cytochrome c from the mitochondria through up-regulation of the pro-apoptotic Bax protein, which results in the activation of procaspase-9 and -3, and the cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, CR389 seems to have multiple mechanisms of anti-proliferative activity through p53-mediated pathways against human ovarian cancer cells. Therefore, we conclude that CR389 is a candidate therapeutic agent for the treatment of human ovarian cancer via the activation of p53.
    Journal of Microbiology and Biotechnology 01/2015; 25(3). DOI:10.4014/jmb.1412.12080
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    ABSTRACT: Bacillus subtilis HK176 with high fibrinolytic activity was isolated from cheonggukjang, a Korean fermented soyfood. A gene, aprE176, encoding the major fibrinolytic enzyme was cloned from B. subtilis HK176 and overexpressed in E. coli BL21(DE3) using plasmid pET26b(+). The specific activity of purified AprE176 was 216.8 +/- 5.4 plasmin unit/mg protein and the optimum pH and temperature were pH 8.0 and 40 degrees C, respectively. Error-prone PCR was performed for aprE176, and the PCR products were introduced into E. coli BL21(DE3) after ligation with pET26b(+). Mutants showing enhanced fibrinolytic activities were screened first using skim-milk plates and then fibrin plates. Among the mutants, M179 showed the highest activity on a fibrin plate and it had one amino acid substitution (A176T). The specific activity of M179 was 2.2-fold higher than that of the wild-type enzyme, but the catalytic efficiency (k(cat)/K-m) of M179 was not different from the wild-type enzyme owing to reduced substrate affinity. Interestingly, M179 showed increased thermostability. M179 retained 36% of activity after 5 h at 45 degrees C, whereas AprE176 retained only 11%. Molecular modeling analysis suggested that the 176th residue of M179, threonine, was located near the cation-binding site compared with the wild type. This probably caused tight binding of M179 with Ca2+, which increased the thermostability of M179.
    Journal of Microbiology and Biotechnology 01/2015; 25(1):89-97. DOI:10.4014/jmb.1409.09087
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    ABSTRACT: 4-O-methylhonokiol (MH), a bioactive compound derived from Magnolia officinalis, is known to exhibit anti-tumor effects in various cancer cells. However, the precise mechanism of its anti-cancer activity in cervical cancer cells has not yet been studied. In this study, we demonstrated that MH induces apoptosis in SiHa cervical cancer cells by enhancing peroxisome proliferator-activated receptor-gamma (PPARγ) activation followed by inhibition of the PI3K/Akt pathway and intrinsic pathway induction. MH upregulated PPARγ and PTEN expression levels while it decreased p-Akt in MH-induced apoptotic process, thereby supporting the fact that MH is a PPARγ activator. Additionally, MH decreased the expression of Bcl-2 and Bcl-XL, inducing intrinsic pathway in MH-treated SiHa cells. Furthermore, MH treatment led to the activation of caspase-3/caspase-9 and proteolytic cleavage of poly ADP ribose polymerase (PARP). The expression levels of Fas (CD95) and E6/E7 oncogenes were not altered by MH treatment. Taken together, MH activates PPARγ/PTEN expressions and induces apoptosis via the suppression of the PI3K/Akt pathway and mitochondria-dependent pathways in SiHa cells. These findings suggest that MH has a potential for development as a therapeutic agent for human cervical cancer.
    Journal of Microbiology and Biotechnology 01/2015; 25(3). DOI:10.4014/jmb.1411.11073
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    ABSTRACT: Use of graphene as a next-generation biomaterial is increasing, particularly with the ever-increasing demand for its use in biomedical applications. Graphene, a new class of one atom thick nanosheets, is a true two-dimensional honeycomb network nanomaterial that has attracted interest in various scientific fields and has rapidly become the most widely studied carbon-based material. Since its discovery in 2004, many reports have explored the potential of graphene because of its unique mechanical, optical, thermal, electronic, and magnetic properties. Graphene-related materials, such as graphene oxide and reduced graphene oxide, have been studied extensively in the biotechnology arena due to their capability for multivalent functionalization and efficient loading of various biomolecules on their surfaces. This review provides a brief summary of the current progress in graphene and graphene oxide biological research and synthesizes recent findings to spark novel applications in biomedicine. Although graphene will continue to provide a fertile ground for new applications, the opportunities and challenges in this rapidly growing area are discussed together with the versatility of these multifaceted materials.
    Journal of Microbiology and Biotechnology 01/2015; 25(2). DOI:10.4014/jmb.1412.12045
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    ABSTRACT: H9 is an ethanol extract prepared from nine traditional/medicinal herbs. This study was focused on the anticancer effect of H9 in non-small cell lung cancer cells. The effects of H9 on cell viability, apoptosis, mitochondrial membrane potential (MMP; ΔΨm), and apoptosis-related protein expressions were investigated in A549 human lung cancer cells. In this study, H9-induced apoptosis was confirmed by propidium iodide (PI) staining, expression levels of mRNA were determined by reverse transcriptase polymerase chain reaction (RT-PCR), protein expression levels were checked by western blot, and MMP (ΔΨm) was measured by JC-1 staining. Our results indicated that H9 decreased the viability of A549 cells and induced cell morphological changes in a dose-dependent manner. H9 also altered expression levels of molecules involved in intrinsic signaling pathway. H9 inhibited Bcl-xl expression, whereas Bax expression was enhanced and cytochrome C was released. Furthermore, H9 treatment led to the activation of caspase-3/caspase-9 and proteolytic cleavage of poly (ADP-ribose) polymerase (PARP); the MMP was collapsed by H9. However, expressions of extrinsic pathway molecules such as Fas/FasL, TRAIL/TRAIL-R, DR5, and Fas associated death receptor (FADD) were downregulated by H9. These results indicated that H9 inhibited proliferation and induced apoptosis by activating intrinsic pathways but not extrinsic pathways in human lung cancer cells. Our results suggest that H9 can be used as a modulating agent in non-small cell lung cancer (NSCLC).
    Journal of Microbiology and Biotechnology 01/2015; 25(3). DOI:10.4014/jmb.1412.12074
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    ABSTRACT: Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured in NMS medium with supply of 7:3 air/methane ratio at 30 °C. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM respectively, for an efficient accumulation of methanol. A 40 mM of sodium formate as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/L·h, titer of 0.393 g methanol/L, and conversion of 73.8 % (mol methanol/mol methane) were obtained at the optimized batch condition.
    Journal of Microbiology and Biotechnology 01/2015; 25(3). DOI:10.4014/jmb.1412.12007
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    ABSTRACT: The interactive inhibitory effects of pH and chloride on the catalysis of laccase from Trametes versicolor were investigated by studying the alteration of inhibition characteristics of sodium chloride at different pHs for the oxidation of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid). At pH 3.0, the addition of sodium chloride (50 mM) brought about a 40-fold increase in K-m(aPP) and a 4-fold decrease in V-max(app). As the pH increased to 7.0, the inhibitory effects of sodium chloride became significantly weakened. The mixed-inhibition mechanism was successfully used to quantitatively estimate the competitive and uncompetitive inhibition strengths by chloride at two different pHs (pH 3.0 and 6.0). At pH 3.0, the competitive inhibition constant, K-i, was 0.35 mM, whereas the uncompetitive inhibition constant, K-i', was 18.1 mM, indicating that the major cause of the laccase inhibition by chloride is due to the competitive inhibition step. At a higher pH of 6.0, where the inhibition of the laccase by hydroxide ions takes effect, the inhibition of the laccase by chloride diminished to a great extent, showing increased values of both the competitive inhibition constant (K-i = 23.7 mM) and uncompetitive inhibition constant (K-i' = 324 mM). These kinetic results evidenced that the hydroxide anion and chloride share a common mechanism to inhibit the laccase activity.
    Journal of Microbiology and Biotechnology 12/2014; 24(12):1673-1678. DOI:10.4014/jmb.1408.08012