Journal of Microbiology and Biotechnology (J Microbiol Biotechnol)

Publisher: Hanʼguk Sanŏp Misaengmul Hakhoe

Journal description

Current impact factor: 1.32

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 1.32
2012 Impact Factor 1.399
2011 Impact Factor 1.381
2010 Impact Factor 1.224
2008 Impact Factor 2.062
2007 Impact Factor 2.062
2006 Impact Factor 2.037
2005 Impact Factor 1.744
2004 Impact Factor 1.663
2003 Impact Factor 1.202
2002 Impact Factor 1.364
2001 Impact Factor 1.338
2000 Impact Factor 1.083
1999 Impact Factor 0.809
1998 Impact Factor 0.436
1997 Impact Factor 0.226

Impact factor over time

Impact factor

Additional details

5-year impact 1.47
Cited half-life 4.60
Immediacy index 0.12
Eigenfactor 0.01
Article influence 0.37
Website Journal of Microbiology and Biotechnology website
ISSN 1017-7825
OCLC 261226927
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publications in this journal

  • Journal of Microbiology and Biotechnology 06/2015; 25(6):910-917. DOI:10.4014/jmb.1411.11075
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    ABSTRACT: Rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonads were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU-67 and P. putida QAU-90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Some such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the PQQ dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized for their possible role in growth promotion was probed in vitro in lettuce while in vivo in rice, bean and tomato plants. The results showed significant differences (p≤0.05) in parameters such as: plant height, fresh weight and dry weight etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence on the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.
    Journal of Microbiology and Biotechnology 05/2015; 25(5).
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    Journal of Microbiology and Biotechnology 04/2015; 25(4):503-510. DOI:10.4014/jmb.1409.09035
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    ABSTRACT: The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression due to its single membrane and high capacity of secretion simplifying downstream processing of secretory proteins. During the last years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of Life Science, such as enzyme production, feed additives, food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as highly promising expression system illuminating strong, chemical- and temperature-inducible and other types of promoters, strategies for ribosome-binding-site utilization and the novel approach of signal peptide selection. Furthermore, we will outline main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we will introduce the latest discoveries of Tat related complexes' structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies improving the yield of intended productions. We expect it to promote strikingly future developments in the optimization and application of this bacterium.
    Journal of Microbiology and Biotechnology 03/2015; 25(7). DOI:10.4014/jmb.1501.01028
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    ABSTRACT: The endo-polygalacturonase gene (endo-pgaA) was cloned from DNA of Aspergillus niger SC323 using the cDNA synthesized by overlapping PCR, and successfully expressed in Saccharomyces cerevisiae EBY100 through fusing α-factor signal peptide of yeast. The full-length cDNA consists of 1113 bp and encodes a protein of 370 amino acids with a calculated molecular mass of 38.8 kDa. After induction by galactose for 48 h, the activity of recombinant endo-PgaA in culture supernatant can reach up to 1448.48 U/mg. The recombinant protein was purified to homogeneity by ammonium sulfate precipitation and gel filtration column chromatography and subsequently characterized. The optimal pH and temperature of the purified recombinant enzyme were 5.0 and 50 °C, respectively. The Michaelis-Menten constant (Km) and maximal velocity (Vmax) of the enzyme for pectin were 88.54 μmol/ml and 175.44 μmol/mg/min, respectively. The enzyme activity was enhanced by Ca(2+), Cu(2+), Na(+) and strongly inhibited by Pb(2+), Mn(2+). The pectin hydrolysates were mainly galacturonic acid and other oligo-galacturonates. Therefore, these characteristics suggest that the recombinant endo-PgaA may be of potential use in food and feed industry.
    Journal of Microbiology and Biotechnology 03/2015; 25(7). DOI:10.4014/jmb.1501.01024
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    ABSTRACT: Recently, we isolated HY253, a novel decahydrofluorene analog, with the molecular structure of 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol from the roots of Aralia continentalis, which is called Dokwhal, the traditional medicinal herb. Also, we reported previously its cytotoxic activity on cancer cell proliferation in human lung cancer A549 and cervical cancer HeLa cells. The current study was aimed to evaluate its detailed molecular mechanisms on cell cycle arrest and apoptotic induction in human hepatocellular carcinoma HepG2 cells. A flow cytometric analysis of HepG2 cells treated with 60 micrometer HY253 revealed appreciable cell cycle arrest at the G1 phase via inhibition of Rb phosphorylation and down-regulation of cyclin D1. Furthermore, using western blots, up-regulation of cyclin-dependent kinase inhibitors, such as p21(CIP1) and p27(KIP1), was associated with this G1 phase arrest. Moreover, TUNEL assay and immunoblottings revealed apoptotic induction in HepG2 cells treated with 100 micrometer HY253 for 24 h, which is associated with cytochrome c release from mitochondria, via down-regulation of anti-apoptotic Bcl-2 protein which in turn resulted in activation of caspase-9 and -3, and proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, we suggest that HY253 may be a potent chemotherapeutic hit compound for treating human liver cancer cells via up-regulation and activation of p53 gene.
    Journal of Microbiology and Biotechnology 02/2015; 25(3). DOI:10.4014/jmb.1501.01069
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    ABSTRACT: Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using cadA over-expressed Escherichia coli (E. coli) strain and various parameters were optimized for the whole-cell biocatalyst at high substrate concentrations resulting in pyridoxal-5'-phosphate (PLP) is critical factor for biotransformation. When 0.025mM PLP and 1.75M lysine in 500mM sodium acetate buffer were used (pH6), 91% of lysine consumption and about 80% conversion to cadaverine was successfully achieved.
    Journal of Microbiology and Biotechnology 02/2015; 25(7). DOI:10.4014/jmb.1412.12052
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    ABSTRACT: 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which is encoded by some bacteria, can reduce the amount of ethylene, a root elongation inhibitor, and stimulate growth of plants under various environmental stresses. The presence of ACC deaminase activity and the regulation of ACC in several rhizospheric bacteria have been reported. The nitrogen-fixing Pseudomonas stutzeri A1501 is capable of endophytic association with rice plants and promotes the growth of rice. However, the functional identification of ACC deaminase has not been performed. In this study, the proposed effect of ACC deaminase in P. stutzeri A1501 was investigated. Genome mining showed that P. stutzeri A1501 carries a single gene encoding ACC deaminase, designated acdS. The acdS mutant was devoid of ACC deaminase activity and was less resistant to NaCl and NiCl2 compared to the wild-type. Furthermore, inactivation of acdS greatly impaired its nitrogenase activity under salt stress conditions. It was also observed that mutation of the acdS gene led to loss of the ability to promote the growth of rice under salt or heavy metal stress. Taken together, this study illustrates the essential role of ACC deaminase, not only in enhancing salt or heavy metal tolerance of bacteria but also in improving the growth of plants, and provides a theoretical basis for studying the interaction between plant growth-promoting rhizobacteria and plant.
    Journal of Microbiology and Biotechnology 02/2015; 25(7). DOI:10.4014/jmb.1412.12053
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    ABSTRACT: Cadaverine (1, 5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, but there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using recombinant Escherichia 35 coli strain that overexpressing the E.coli MG1655 cadA gene and various parameters were 36 investigated for the whole-cell bioconversion of lysine to cadaverine. The high concentration of lysine resulting that the synthesis of pyridoxal-5'-phosphate (PLP) and it was found that a critical control factor for the biotransformation of lysine to cadaverine. When 0.025 mM PLP and 1.75 M lysine in 500 mM sodium acetate buffer (pH6) were used, 91% of consumption and about 80% conversion of lysine to cadaverine were successfully achieved.
    Journal of Microbiology and Biotechnology 02/2015;
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    ABSTRACT: The filamentous fungus Aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. To facilitate higher yields of proteins of interest, we constructed the pAsOP vector to express heterologous proteins in A. oryzae. pAsOP carries a selectable marker, pyrG, derived from Aspergillus nidulans, and a strong promoter and a terminator of the amyB gene derived from A. oryzae. pAsOP transformed A. oryzae efficiently via the PEG-CaCl2 mediated transformation method. As proof of concept, green fluorescent protein (GFP) was successfully expressed in A. oryzae transformed by pAsOP-GFP. Additionally, we identified a novel fungal α-amylase (PcAmy) gene from Penicillium sp. and cloned the gene into the vector. After transformation by pAsOP-PcAmy, the α-amylase PcAmy from Penicillium sp. was successfully expressed in a heterologous host system for the first time. The α-amylase activity in the A. oryzae transformant was increased by 62.3% compared to the untransformed A. oryzae control. The PcAmy protein produced in the system had an optimum pH at 5.0 and an optimum temperature at 30degrees C. As a cold-adapted enzyme, PcAmy shows potential value in industrial applications because of its high catalytic activity at low temperature. Furthermore, the expression vector reported in this study provides promising utility for further scientific research and biotechnological applications.
    Journal of Microbiology and Biotechnology 02/2015; 25(7). DOI:10.4014/jmb.1410.10022
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    ABSTRACT: VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). Percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1 indicating that VvpM-mediated cell death occurs through necroptosis. Appearance of autophagic vesicles and lipidated form of light chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, a multifarious actions of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.
    Journal of Microbiology and Biotechnology 02/2015; 25(2). DOI:10.4014/jmb.1501.01007