Journal of Microbiology and Biotechnology (J Microbiol Biotechnol)

Publisher: Hanʼguk Sanŏp Misaengmul Hakhoe

Journal description

Current impact factor: 1.53

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2014 Impact Factor 1.525
2013 Impact Factor 1.32
2012 Impact Factor 1.399
2011 Impact Factor 1.381
2010 Impact Factor 1.224
2008 Impact Factor 2.062
2007 Impact Factor 2.062
2006 Impact Factor 2.037
2005 Impact Factor 1.744
2004 Impact Factor 1.663
2003 Impact Factor 1.202
2002 Impact Factor 1.364
2001 Impact Factor 1.338
2000 Impact Factor 1.083
1999 Impact Factor 0.809
1998 Impact Factor 0.436
1997 Impact Factor 0.226

Impact factor over time

Impact factor

Additional details

5-year impact 1.54
Cited half-life 5.50
Immediacy index 0.22
Eigenfactor 0.01
Article influence 0.37
Website Journal of Microbiology and Biotechnology website
ISSN 1017-7825
OCLC 261226927
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publications in this journal

  • Journal of Microbiology and Biotechnology 09/2015; 25(9):1460-1466. DOI:10.4014/jmb.1502.02034
  • Journal of Microbiology and Biotechnology 09/2015; 25(9):1401-1409. DOI:10.4014/jmb.1502.02029
  • Journal of Microbiology and Biotechnology 09/2015; 25(9):1568-1577. DOI:10.4014/jmb.1501.01077
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    ABSTRACT: A total of 646 strains, including green algae and diatoms, were isolated from 220 samples to screen microalgae with high lipid productivity (LP). The samples were obtained from nine habitats in Northern Xinjiang, China in June 2013. This study initially identified eight lipidrich strains, namely, Desmodesmus intermedius XJ-498, D. intermedius XJ-145, D. intermedius XJ- 99, Monoraphidium pusillum XJ-489, M. dybowskii XJ-435, M. dybowskii XJ-151, Mychonastes homosphaera XJ-488, and Podohedriella falcata XJ-176, based on 18S rDNA sequencing. The strains were cultured in a photobioreactor for the same period. Results showed that the specific growth rate (day-1) of M. pusillum XJ-489 was the highest (1.14 ± 0.06), and the biomass concentration (g/l) of D. intermedius XJ-99 was the highest (2.84 ± 0.3). Futhermore, the lipid content (%) of M. dybowskii XJ-151 was the highest (33.5 ± 4.38), and the lipid productivity (mg l-1 day-1) of My. homosphaera XJ-488 was the highest (86.41 ± 9.04). C16 to C18 accounted for 86% to 98% of the total lipid, and the biodiesel qualities of the selected algae corresponded to international standards. This study suggests that My. homosphaera XJ-488, D. intermedius XJ-99, and M. dybowskii XJ-151 are the most potential strains for biodiesel production among all the isolated strains. © 2015 by The Korean Society for Microbiology and Biotechnology.
    Journal of Microbiology and Biotechnology 06/2015; 25(6):910-917. DOI:10.4014/jmb.1411.11075
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    ABSTRACT: Rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonads were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU-67 and P. putida QAU-90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Some such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the PQQ dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized for their possible role in growth promotion was probed in vitro in lettuce while in vivo in rice, bean and tomato plants. The results showed significant differences (p≤0.05) in parameters such as: plant height, fresh weight and dry weight etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence on the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.
    Journal of Microbiology and Biotechnology 05/2015; 25(5).
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    ABSTRACT: The iLOV protein belongs to a family of blue-light photoreceptor proteins containing a lightoxygen- voltage sensing domain with a noncovalently bound flavin mononucleotide (FMN) as its chromophore. Owing to advantages such as its small size, oxygen-independent nature, and pH stability, iLOV is an ideal candidate over other reporter fluorescent proteins such as GFP and DsRed. Here, for the first time, we describe the feasibility of applying LOV domain-based fluorescent iLOV as a metal sensor by measuring the fluorescence quenching of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the inherent copper sensing property of the iLOV protein and identified the possible amino acids responsible for metal binding. The fluorescence quenching upon exposure to Cu2++ was highly sensitive and exhibited reversibility upon the addition of the metal chelator EDTA. The copper binding constant was found to be 4.72 ± 0.84 μM. In addition, Cu2+-bound iLOV showed high fluorescence quenching at near physiological pH. Further computational analysis yielded a better insight into understanding the possible amino acids responsible for Cu2++ binding with the iLOV protein. © 2015 by The Korean Society for Microbiology and Biotechnology.
    Journal of Microbiology and Biotechnology 04/2015; 25(4):503-510. DOI:10.4014/jmb.1409.09035
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    ABSTRACT: Tuberculosis (TB) is an infectious disease transmitted by aerosol droplets characterized by forming granulomatous lesions. Although the number of people infected in the population is high, the vast majority does not exhibit symptoms of active disease and only 5 - 10% develops the disease after a latent period that can vary from weeks to years. The bases of the immune response for this resistance are unknown, but it depends on a complex interaction between the environment, the agent and the host. The analysis of cellular components of M. tuberculosis shows important host-pathogen interactions, metabolic pathways, virulence mechanisms and mechanisms of adaptation to the environment. However the M. tuberculosis proteome still remains largely uncharacterized in terms of virulence and pathogenesis. Here we summarize some of the major proteomic studies performed to scrutinize all the mycobacterial components.
    Journal of Microbiology and Biotechnology 03/2015; 25(8). DOI:10.4014/jmb.1502.02008
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    ABSTRACT: The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression due to its single membrane and high capacity of secretion simplifying downstream processing of secretory proteins. During the last years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of Life Science, such as enzyme production, feed additives, food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as highly promising expression system illuminating strong, chemical- and temperature-inducible and other types of promoters, strategies for ribosome-binding-site utilization and the novel approach of signal peptide selection. Furthermore, we will outline main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we will introduce the latest discoveries of Tat related complexes' structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies improving the yield of intended productions. We expect it to promote strikingly future developments in the optimization and application of this bacterium.
    Journal of Microbiology and Biotechnology 03/2015; 25(7). DOI:10.4014/jmb.1501.01028
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    ABSTRACT: The endo-polygalacturonase gene (endo-pgaA) was cloned from DNA of Aspergillus niger SC323 using the cDNA synthesized by overlapping PCR, and successfully expressed in Saccharomyces cerevisiae EBY100 through fusing α-factor signal peptide of yeast. The full-length cDNA consists of 1113 bp and encodes a protein of 370 amino acids with a calculated molecular mass of 38.8 kDa. After induction by galactose for 48 h, the activity of recombinant endo-PgaA in culture supernatant can reach up to 1448.48 U/mg. The recombinant protein was purified to homogeneity by ammonium sulfate precipitation and gel filtration column chromatography and subsequently characterized. The optimal pH and temperature of the purified recombinant enzyme were 5.0 and 50 °C, respectively. The Michaelis-Menten constant (Km) and maximal velocity (Vmax) of the enzyme for pectin were 88.54 μmol/ml and 175.44 μmol/mg/min, respectively. The enzyme activity was enhanced by Ca(2+), Cu(2+), Na(+) and strongly inhibited by Pb(2+), Mn(2+). The pectin hydrolysates were mainly galacturonic acid and other oligo-galacturonates. Therefore, these characteristics suggest that the recombinant endo-PgaA may be of potential use in food and feed industry.
    Journal of Microbiology and Biotechnology 03/2015; 25(7). DOI:10.4014/jmb.1501.01024
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    ABSTRACT: Recently, we isolated HY253, a novel decahydrofluorene analog, with the molecular structure of 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol from the roots of Aralia continentalis, which is called Dokwhal, the traditional medicinal herb. Also, we reported previously its cytotoxic activity on cancer cell proliferation in human lung cancer A549 and cervical cancer HeLa cells. The current study was aimed to evaluate its detailed molecular mechanisms on cell cycle arrest and apoptotic induction in human hepatocellular carcinoma HepG2 cells. A flow cytometric analysis of HepG2 cells treated with 60 micrometer HY253 revealed appreciable cell cycle arrest at the G1 phase via inhibition of Rb phosphorylation and down-regulation of cyclin D1. Furthermore, using western blots, up-regulation of cyclin-dependent kinase inhibitors, such as p21(CIP1) and p27(KIP1), was associated with this G1 phase arrest. Moreover, TUNEL assay and immunoblottings revealed apoptotic induction in HepG2 cells treated with 100 micrometer HY253 for 24 h, which is associated with cytochrome c release from mitochondria, via down-regulation of anti-apoptotic Bcl-2 protein which in turn resulted in activation of caspase-9 and -3, and proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, we suggest that HY253 may be a potent chemotherapeutic hit compound for treating human liver cancer cells via up-regulation and activation of p53 gene.
    Journal of Microbiology and Biotechnology 02/2015; 25(3). DOI:10.4014/jmb.1501.01069
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    ABSTRACT: Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using cadA over-expressed Escherichia coli (E. coli) strain and various parameters were optimized for the whole-cell biocatalyst at high substrate concentrations resulting in pyridoxal-5'-phosphate (PLP) is critical factor for biotransformation. When 0.025mM PLP and 1.75M lysine in 500mM sodium acetate buffer were used (pH6), 91% of lysine consumption and about 80% conversion to cadaverine was successfully achieved.
    Journal of Microbiology and Biotechnology 02/2015; 25(7). DOI:10.4014/jmb.1412.12052
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    ABSTRACT: 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which is encoded by some bacteria, can reduce the amount of ethylene, a root elongation inhibitor, and stimulate growth of plants under various environmental stresses. The presence of ACC deaminase activity and the regulation of ACC in several rhizospheric bacteria have been reported. The nitrogen-fixing Pseudomonas stutzeri A1501 is capable of endophytic association with rice plants and promotes the growth of rice. However, the functional identification of ACC deaminase has not been performed. In this study, the proposed effect of ACC deaminase in P. stutzeri A1501 was investigated. Genome mining showed that P. stutzeri A1501 carries a single gene encoding ACC deaminase, designated acdS. The acdS mutant was devoid of ACC deaminase activity and was less resistant to NaCl and NiCl2 compared to the wild-type. Furthermore, inactivation of acdS greatly impaired its nitrogenase activity under salt stress conditions. It was also observed that mutation of the acdS gene led to loss of the ability to promote the growth of rice under salt or heavy metal stress. Taken together, this study illustrates the essential role of ACC deaminase, not only in enhancing salt or heavy metal tolerance of bacteria but also in improving the growth of plants, and provides a theoretical basis for studying the interaction between plant growth-promoting rhizobacteria and plant.
    Journal of Microbiology and Biotechnology 02/2015; 25(7). DOI:10.4014/jmb.1412.12053