Shanghai kou qiang yi xue = Shanghai journal of stomatology

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    ABSTRACT: To evaluate the bond strength of Vita OMEGA 900 low fusing porcelain fused with the goldplated Wirobond cobalt-chrome metalt ceramic alloy. Low fusing porcelain was fused with the cobalt-chromium alloy strips(group A) and the goldplated cobalt-chromium alloy strips(group B) respectively according to ISO9693 (A:8,B:10). 8 specimens of each group were submitted to three point bending test. Two more test pieces fused with gold plated cobalt-chromium alloys were made (group B'). One test piece of both group B and group B' were observed under scanning electron microscope (SEM) randomly. The data was analyzed with SPSS 16.0 software package. The bond strength (MPa) of group A and group B was 29.92±4.28 and 28.20±5.21, respectively (P>0.05), both higher than 25 MPa required by ISO9693. SEM showed that Vita OMEGA 900 low fusing porcelain and the goldplated Wirobond cobalt-chrome metalt ceramic alloy combined together closely without cracks. Much gold was fused to the cobalt-chrome alloy surface of breaking porcelain specimen after testing. Vita OMEGA 900 low fusing porcelain can match with the goldplated Wirobond cobalt-chrome metalt ceramic alloy. Supported by Foundation of Education Department of Jiangxi Province (GJJ10367).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):35-38.
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    ABSTRACT: To investigate the expression of gene DEK in oral squamous cell carcinoma, and explore the role of DEK in the development and metastasis of oral squamous cell carcinoma. Thirty-three specimens of oral squamous cell carcinoma (OSCC), 28 specimens of cervical lymph nodes metastasis, and 33 specimens of normal oral mucosal tissues adjacent to the cancerous lesions were examined. Polymerase chain reaction with reverse transcription (RT-PCR) and immunohistochemistry were used to detect mRNA and protein expression of DEK, respectively.The results were analysed with SPSS17.0 software package. RT-PCR and immunohistochemistry showed that the expression of DEK mRNA and DEK protein in metastatic lymph nodes (82.1%, 82.1%) and cancerous tissues (81.2%, 76.8%) of OSCC were significantly higher than in the adjacent tissues (27.3%, 18.2%). The difference between cancerous tissues and adjacent tissues was significant (P<0.05). DEK may play an important role in the occurrence and metastasis of OSCC.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):75-79.
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    ABSTRACT: To investigate the value of using con-beam computer tomography (CBCT) to evaluate bone volume in the edentulous area of posterior maxilla, and guide sinus floor elevation. Twenty eight patients who planed to receive dental implant in the posterior maxilla underwent CBCT scan. After analysis of residual bone height, 19 patients with 5-10 mm bone height below the sinus floor underwent transcrestal sinus floor elevation, while 7 patients with less than 5 mm bone height below the sinus floor underwent lateral window sinus floor elevation. The treatment outcomes were assessed postoperatively. CBCT detected bone deficiency in the posterior maxillary alveolar bone in 26 patients, mucus cyst of maxillary sinus in 1 patient and sinus septa in 2 patients. Nineteen patients underwent transcrestal sinus floor elevation, and 7 patients underwent lateral window sinus floor elevation. All patients completed final dental restorations, and the restorations achieved satisfactory function and esthetic. CBCT imaging plays an important role in determining bone quality in posterior maxilla and guiding sinus floor elevation. Supported by Social Undertakings Technological Innovation and Demonstration Program of Nantong City (HS2011035).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):71-74.
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    ABSTRACT: To evaluate the clinical outcome and osteogenesis of simultaneous endosseous implant placements with xenographic bone grafts in horizontal bone defect. Ten patients underwent simultaneous endosseous implant placements with xenographic bone grafts from January, 2010 to June, 2012. These patients were scanned by cone-beam computed tomography immediately, 3 months and 6 months after operation, respectively. The alveolar ridge thickness were measured in different sites, and the data with normal distribution was analyzed with SPSS17.0 software package. With increase of xenographic bone graft retention time, the difference of osteogenesis of xenographic bone grafts was significantly different (P<0.05). Cone-beam computed tomography can clearly determine the clinical outcome and osteogenesis of simultaneous endosseous implant placements with xenographic bone grafts in horizontal bone defect, which is characterized by good ossification. Supported by Innovative Science and Technology Program of Central Hospital of Xuzhou City (XZS2012071).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(20):66-70.
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    ABSTRACT: The purpose of this study was to evaluate the effect of sand-blasted and acid-etched titanium surface on MC3T3-E1 murine pre-osteoblast cell differentiation, and investigate the pathway of regulating osteogenic differentiation of MC3T3-E1 cells on sand-blasted and acid-etched titanium surface in order to elucidate the regulatory mechanisms of surface roughness on osteoblastic differentiation. The characteristic of PT polished titanium (PT), sand-blasted and acid-etched (SLA) titanium surface were examined by scanning electron microscopy (SEM). Real-time PCR was applied to detect the expression of osteogenic genes including Runx2, OSX, OCN and OPN of the MC3T3-E1 cells cultured on the 2 groups of substrates.ERK1/2 activities in MC3T3-T1 cells were measured by Western blot on SLA surface. The data was analyzed using SAS9.0 software package. The result of SEM observation showed that the PT surface was turned titanium surfaces with the mean peak to valley roughness (Ra) of 0.2 μm and the corresponding Ra value of SLA was 3.2 μm. The expression levels of Runx2, OSX, OPN and OCN were significantly higher and the cell proliferation was significantly lower on SLA surfaces than on PT surfaces (P<0.05). The expression levels of Runx2, OSX, OPN and OCN were up-regulated by the effect of SLA surface with PD98095. Compared with PT surface, ERK1/2 phosphorylation was continuously inhibited by SLA. Moreover, PT surfaces treated with PD98095 and SLA surfaces without PD98095 both demonstrated reduced ERK1/2 phosphorylation of the cells and the inhibitive effect of SLA surfaces was milder than that of PD98095. Surface roughness is an important factor that determines osteoblast behaviors. Surface roughness of titanium substrates seems to enhance the osteoblastic differentiation of MC3T3-E1 cells and the enhancing effect of surface roughness on cell differentiation may be mediated by suppressing the activity of ERK1/2 pathway.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):39-45.
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    ABSTRACT: To explore the electroencephalogram (EEG) changes after occlusal trauma in rats. Health Wistar rats were divided into 2 groups: traumatic occlusion group (1-day, 3-day, 7-day, 14-day, 28-day group) and control group. The electrodes were inserted in the hippocampus of anesthetized rats guided by stereotaxic apparatus of brain and then the hippocampal EEG were recorded during sleep. Data were analysed by SPSS 17.0 software package for ANOVA. The δ waves were increased and the frequency and amplitude of EEG were significantly reduced in 1-day traumatic group(P<0.05); The waves were decreased and the frequency and amplitude of EEG were significantly increased in 3-day traumatic group(P<0.05). There was no significant difference compared with the control group. The δ waves were decreased in 7-day and 14-day traumatic group and the frequency and amplitude of EEG were significantly increased (P<0.05). The indexes in the 28-day traumatic group returned to normal. CONCLUSIONS:Occlusal trauma can change the EEG of the rat during sleep. It mainly affects the deep sleep, and reduces sleep quality. Supported by Innovation Fund of Shandong University (2010GN048).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):26-29.
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    ABSTRACT: PURPOSE:To investigate the effects of injection of botulium toxin type A at trigger point for treatment of patients with primary trigeminal neuralgia. METHODS:Sixteen patients with primary Trigeminal Neuralgia were treated with injection of botulium toxin type A. Visual analog scores(VAS) at 1 week, 2 weeks, 1 month, 3months and 6 months after treatment and Barrow Neurological Institute (BNI) pain evaluation criteria were utilized to measure the degree of pain. The data was analyzed with SPSS 10.0 software package. The VAS score was 9.12±0.65 before botulium toxin type A injection while the scores were 2.8±1.36, 2.2±1.26, 1.3±1.45, 1.3±1.45 and 1.2±2.52 at 1 week, 2 weeks, 1 month,3 months and 6 months after treatment. There was significant difference in VAS compared with before treatment. VAS score was lower and stable at 1 month, 3 months and 6 months after treatment, but no significant difference was found at 1-week and 2-week after treatment. BNI evaluation results showed good therapeutic effect 1 week after treatment, while the best therapeutic effect was noted 1-3 months after treatment. 6 months later, 1 patient had recurrence and 11 patients had complete relief of pain. Botulium toxin type A injection is an effective way for treatment of patients with primary trigeminal neuralgia.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):117-119.
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    ABSTRACT: To evaluate the clinical effect of modified casting post with zirconia crown in restoration of anterior teeth. A total of 45 anterior teeth (32 patients) were treated with modified gold-alloy post-cores with zirconia crowns. All roots were greatly tilted to the labial side. Marginal integrity, anatomic form, surface, color and the gingival situation after restorations were assessed at 6 and 12 months with the California Dental Association (CDA) quality assessment system. The clinical outcome of 44 zirconia crowns achieved rank A (97.8%), while the gingival situation in one patient (2.2%) reached rank B. Modified gold-alloy post-core with zirconia crown contributes to the aesthetic effect of the anterior teeth which need to change the angle.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):83-86.
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    ABSTRACT: Nasal glioma, which is also known as nasal glial heterotopia, is a rare benign congenital lesion. A lot of explanations for the pathogenesis of this disease have already been provided. However, all of them lack theoretical basis. Nowadays, for nasal glioma, complete resection of the tumor is generally used in clinic treatment. CT examination or MRI is necessary for confirming the lesions and the relation between the tumor and intracranial part. This paper reported a neonatal nasal glioma case associated with congenital nasal deformity.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):126-128.
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    ABSTRACT: The aim of this study was to evaluate the clinical benefit of valacyclovir when performing full-mouth periodontal debridement in patients with advanced chronic periodontitis. Fifty-nine patients with advanced chronic periodontitis were randomly assigned into control-treatment group(n=29) and intensive-treatment group(n=30). All patients were given instructions of basic oral hygiene and a standard cycle of supragingival mechanical scaling and polishing. Patients in the intensive-treatment group received oral valacyclovir for 1 week, while patients in the control-treatment group received placebo. Thereafter, patients in both groups underwent full-mouth intensive removal of subgingival dental plaque biofilms with the use of scaling and root planing within 48 hours. Periodontal parameters were evaluated before treatment and 2 or 6 months after treatment. The data was statistically analyzed using SPSS17.0 software package. No significant difference in clinical parameters was noted before treatment. 2 and 6 months after treatment, the mean percentage reduction of sites with BOP and PD≥4 mm were significantly higher in the intensive-treatment group than in the control-treatment group (P<0.05). Similarly, patients in the intensive-treatment group had higher mean PD reduction than those in the control-treatment group 2 months (P<0.05) and 6 months after therapy (P<0.05). However, the mean values of CAL reduction were slightly and not significantly higher in the intensive-treatment group than in the control-treatment group after therapy. It may be concluded that valacyclovir significantly improves clinical results of full-mouth non-surgical periodontal debridement in advanced chronic periodontitis.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):103-106.
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    ABSTRACT: To investigate the temporal and special expressions and functions of perlecan in dental pulp of rats after injury. Specimens of one side of maxillary molars of 24 male SD rats were collected 1,3,5,7 days after injury, and the other side were used as controls. The specimens were decalcified and embedded in paraffin, sliced by longitudinal section and stained by immunohistochemistry. The staining of perlecan and β1 integrin were examined, and the average optical density (AOD) was compared. The measurement data were analyzed by ANOVA using SPSS 19.0 software package. The staining intensity of perlecan in dental pulp in the experimental group was significantly higher compared with that of the control group. The immunolocalization of perlecan in dental pulp was gradually progressed as the inflamed tissue was progressing after injury, and its AOD was significantly higher than that of control group (F=11.104,P>0.05), especially in the group of 1 d. The staining of β1 integrin was generally enhanced in the pulp 3 d after injury, and its AOD was significantly higher than that of control group (F=124.18,P>0.05). Perlecan may play an important role in tissue defense mechanism after injury through regulating angiogenesis and inflammatory cell infiltration. Supported by Science and Technology Research Project of Department of Education of Zhejiang Province (20051196) and Wenzhou City (Y20100015).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):15-19.
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    ABSTRACT: To study the effect of fluor protector against demineralization of deciduous teeth enamel surface in milk beverages, to certify the mechanism of anti-demineralization of fluor protector, and provide laboratory basis for clinical practice of fluor protector and the prevention of infants and young children. The enamel surfaces of 30 prepared deciduous teeth without caries were randomly divided into 3 groups. Group A was control group. Milk beverages were used in group B, and 0.1% fluor protector was used in group C. Each group included 10 specimens. All specimens were soaked in water tank at 37 degrees centigrade for 4 days, but specimens in groups B and C were soaked at intervals in 10mL yogurt drinks in water tank at 37 degrees centigrade, 9 minutes for one time and 3 times for one day. Scanning electron microscope (SEM) and spectrum analyzer were used to detect the changes in surface morphology of deciduous teeth enamel surface. The concentration values and the ratio of calcium/phosphorus were analyzed with SPSS11.5 software package. Enamel soaked in milk beverages had decreased Ca(2+) and P(3+) content significantly (P<0.01). Ca(2+) and P(3+) content in enamel in group C was significantly higher than in group B (P<0.01). Scanning electron microscopy showed enamel surface in group C had mild demineralization. Milk beverages can cause a strong acid demineralization in deciduous teeth enamel surface. The application of fluor protector in enamel surface can inhibit enamel demineralization in the milk beverages.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):51-57.
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    ABSTRACT: To investigate the relationship between impaction of maxillary anterior teeth and sagittal facial type and evaluate the dentofacial morphological characteristics of patients with maxillary teeth impaction. Totally 90 patients with maxillary anterior teeth impaction were divided into 3 groups (one incisor impaction, one canine impaction and two canines impaction), and their cephalometric films were measured and analyzed. They were further divided into Class I, II and III facial types according to ANB and the constituent ratio were calculated. SPSS 17.0 software package was applied for Student's t test and chi-square test. SNA, A'-Ptm' and L1-NB were smaller than the normal value in the 3 groups. Convexity, L1-MP, ANB and Wits appraisal were smaller while AB plane angle, U1-NA and U1-NA were greater than the normal value in groups of one and two canines impaction; S'-Ptm', L1-NB were smaller while U1-L1 was greater than normal value in group of two canines impaction; Among the 3 groups, ANB and Wits appraisal were the smallest while AB plane angle was the greatest in group of two canines impaction. The sagittal facial type of 90 patients was mainly Class I (50%), but Class III in group of two canines impaction increased to 40%. Impacted maxillary anterior teeth might result in short maxillary basal bone. One canine impaction has the greatest influence than one incisor impaction on sagittal position of jaws. Two canines impaction has the greatest impact on sagittal facial type and tends to be Class III facial type.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):99-102.
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    ABSTRACT: To investigate the effect of N-glycosylated E-cadherins on proliferation and invasiveness of tongue squamous cell carcinoma CAL 27 cells, and to study the effect of hypoglycosylation of E-cadherins on the stability of adherens junctions (AJs) mediated by E-cadherins. Human tongue squamous cell carcinoma CAL 27 cells were respectively transfected with the plasmids encoding either hypoglycosylated or wild-type E-cadherins with FLAG as tags. Western blot of FLAG was used to detect the expression of exogenous E-cadherins in the transfected cells after 48 hours. The cell proliferation counting, monolayer cell scratch wound healing and in vitro cell invasiveness assays were performed to evaluate the proliferation and invasiveness of cells. Immunoprecipitation experiments were used to assay the quantity of α-catenins, β-catenins, γ-catenins and vinculins linked with the cytosolic tails of E-cadherins. All statistical analysis were performed using SPSS 17.0 software package. The expression of exogenous E-cadherins was detected in transfected CAL 27 cells by Western blot. Cell proliferation counting assay revealed that hypoglycosylated E-cadherins significantly inhibited proliferation of CAL 27 cells, compared with wild-type E-cadherins. The monolayer cell scratch wound healing and in vitro cell invasiveness assays indicated that hypoglycosylated E-cadherins also significantly restrained both invasiveness of CAL 27 cells, compared with wild-type E-cadherins. The immunoprecipitation experiments demonstrated that hypoglycosylated E-cadherins exhibited an increased association with α-catenins, β-catenins, and γ-catenins and vinculins. Compared with wild-type E-cadherins, hypoglycosylated E-cadherins can significantly suppress proliferation and invasiveness of tongue squamous cell carcinoma CAL 27 cells, and the mechanism was that hypoglycosylated E-cadherins can induce more stable AJs than wild-type E-cadherin. Supported by Scientific Research Foundation for the Returned Oversea Chinese Scholars, State Ministry of Education of China and Shandong Provincial Award Foundation for Excellent Middle- and Young-Aged Scientists (BS2010YY021).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):1-6.
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    ABSTRACT: To study the feasibility of tissue engineered bone constructed with simvastatin carried by PLGA/CPC and bone marrow stromal cells (BMSCs) and screen the effective drug loading of simvastatin. Solvent casting-particle leaching technology combined with the phase separation process was used to prepare the different concentrations (simvastatin mass: 0.1, 0.5, 1 mg) of simvastatin carried by PLGA/CPC composite scaffold materials. Scanning electron microscopy was used to observe the porosity and drug release curve was drawn; Alizarin red staining and type I collagen staining were applied to observe the effect of osteogenic medium and simvastatin on the role of BMSCs to the osteogenetic differentiation. The induced passage 3 cells after dil staining were mixed with the composite scaffold material to a complex. Scanning electron microscopy and laser confocal microscope were used to observe the adhesion on the complex. CCK-8 and alkaline phosphatase (ALP) were applied to observe the proliferation and differentiation. SPSS 18.0 software package was used for statistical analysis. The scaffold porosity was more than 90% with an average aperture of 200-300 μm. The drug released slowly. There was no obvious interpretation. Type I collagen showed positive expression. Alizarin red staining proofed the formation of mineralization nodules in group which was induced with the conditional medium and simvastatin. 0.5 mg group showed cells adhered to the inner surface of the scaffold material and could significantly promote the proliferation and differentiation of cells. Combination of simvastatin and osteogenic medium can effectively promote the differentiation of BMSCs. Simvastatin carried by PLGA/CPC scaffold materials is an ideal tissue engineering scaffold material. PLGA/CPC scaffold containing 0.5 mg simvastatin can effectively promote the proliferation and differentiation of BMSCs. Supported by Natural Science Foundation of Jilin Province (201215052).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):7-14.
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    ABSTRACT: To assess the impact of individual nursing care on aged patients who underwent tooth extraction in outpatient clinic. One hundred aged patients who required tooth extraction were allocated into the experimental group (with individual nursing care before extraction) or the control group (blank control). The level of patients' anxiety was evaluated by HAMA scale before and after extraction. The pain state was evaluated by VRS scale after extraction. The date was analyzed for student's t test and ANONA by SPSS 16.0 software package. Both the anxious score and VRS score after extraction in the experimental group were significantly lower than that of the control group (P<0.05). For aged patients who underwent tooth extraction, individual nursing care can alleviate the level of their anxiety, which is helpful to improve their reliance and reduce complications.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):113-116.
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    ABSTRACT: The advanced dental local anesthesia technique of Single Tooth Anesthesia (STA) was widely used in the department of oral surgery in major dental schools in China. This technology had been developed to assist the dentist to provid enhanced pain relief with reduced injection pain and fewer adverse effects, and also help the patient to relax from fear. In our department, STA technology has been used in the clinical teaching, and widely accepted by the dentists, students and patients. This paper discussed the experiences and problems of the application of STA in clinical teaching. Supported by National Natural Science Foundation of China (8102054).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):107-109.
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    ABSTRACT: Porcelain veneer had gained more and more attention in dental clinical applications due to its advantages such as good esthetic effects and minor invasiveness. The reliable and consistent adhesive bonding were the key to success. The enamel which featured high mineralization and low moisture would be the ideal bonding part for porcelain veneer. This article was aimed to summarize the research progress regarding to those factors that might had effect on the bonding strength between the porcelain veneer and the enamel including the restoration types of resin adhesives and bonding surface preparations.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):120-125.
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    ABSTRACT: To compare the expression of CD(4+) T cells in mice periodontitis induced by two periodontal pathogen strains, and evaluate the role of CD(4+) T cells in periodontitis. Twelve C57BL/6 mice were divided into 3 groups at random including sham-infected, Porphyromonas gingivalis (P. gingivalis) W83-infected and P. gingivalis ATCC 33277-infected groups. All mice were sacrificed at the 4th week after the last infection. The analysis of alveolar bone resorption and the examination of histological staining were performed. The population of CD(4+) T cells in the gingivae and the cervical lymph nodes (CLNs) were analyzed by flow cytometry. SPSS 11.0 software package was used for statistical analysis. The levels of alveolar bone resorption were significantly high in both P. gingivalis W83-infected and P. gingivalis ATCC 33277-infected mice compared to sham-infected mice. Histological staining showed that the loss of periodontal attachment and osteoclasts-mediated alveolar bone resorption were found in both P. gingivalis W83-infected and P. gingivalis ATCC 33277-infected mice. Moreover, the periodontal destruction was severer in P. gingivalis W83-infected mice than in P. gingivalis ATCC 33277-infected mice. Flow cytometry showed that the percentage of CD(4+) T cells in the gingivae and CLNs in both P. gingivalis W83-infected and P. gingivalis ATCC 33277-infected mice were significantly higher than that in sham mice, and P. gingivalis W83-infected mice had a higher percentage of CD(4+) T cells compared to P. gingivalis ATCC 33277-infected mice. The population of CD(4+) T cells participates in the pathogenesis of periodontitis, and closely correlates with the periodontal destruction induced by periodontal pathogen infection. Supported by Higher School Specialized Research Fund for Doctoral Program(20112104110013) and Science and Technology Plan of Liaoning Province(2012225015).
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):20-25.
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    ABSTRACT: The purpose of this clinical evaluation was to assess the clinical effect of application of everStick Ortho glass fiber-reinforced composites for orthodontic lingual retainer. A total of 12 patients underwent orthodontic treatment using glass fiber-reinforced composites for fixed orthodontic lingual retainers. During a 24-month follow-up period after application of retainers, the condition of lingual retainers and the periodontal status were examined every 6 months. Gingival index and sulcus bleeding index were analyzed by nonparametric tests with SPSS 13.0 software package. During 24-month follow-up period, 11 orthodontic lingual retainers were stable without fracture while the periodontal tissue revealed no significant changes in all 12 patients. The application of everStick Ortho glass fiber-reinforced composites for orthodontic lingual retainer is a viable alternative to conventional retainer used in orthodontic treatment.
    Shanghai kou qiang yi xue = Shanghai journal of stomatology 02/2014; 23(1):80-82.