Journal of parasitic diseases Impact Factor & Information

Publisher: Springer Verlag

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ISSN 0975-0703

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Springer Verlag

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Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Giardiasis is a prevailing intestinal disease in children. This study aimed to determine molecular prevalence of Giardia intestinalis in children attending Cairo University Pediatrics Hospitals, using copro-PCR assays, conventional methods and to evaluate diagnostic effectiveness of used tests. 229 fecal samples were collected from children suffering from gastrointestinal symptoms and examined for Giardia by microscopy, Immuno-chromatographic test (ICT), copro-DNA using two PCR assays targeting tpi [nested-PCR (nPCR)] and 18S [conventional-PCR (cPCR)] genes. Out of 229 samples assessed, Giardia was diagnosed in 13.9, 17, 17.9, 4.8 % of cases using microscopy, ICT, nPCR (tpi) and cPCR (18S), respectively. Nominating both PCR assays as composite reference standard, microscopy and ICT were of reliable specificity (100 and 96.9 %) and accuracy (95.6 and 93.6 %) but of limited sensitivity (78.6 and 76.2 %). Kappa agreement showed, there was substantial agreement of ICT (0.776) and almost perfect agreement of microscopy (0.839) with PCR assays. Giardia showed a molecular prevalence of 18.3 % (42/229). ICT assay for Giardia surpassed microscopy but both couldn’t be used as a consistent single detection method due to their lowered sensitivities. nPCR targeting tpi is a reliable diagnostic test aiding to determine true prevalence of Giardia.
    Journal of parasitic diseases 09/2015; DOI:10.1007/s12639-015-0714-9
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    ABSTRACT: A total of 193 sera samples, along with tissues (lung, heart, and brain) collected from 136 sheep and 57 goats from the Corporation slaughter house, Madras Veterinary College teaching hospital, and private mutton shops from Chennai were tested for Toxoplasma gondii. All the sera samples were tested using modified direct agglutination test (MAT). Of the 193 sera samples, 57 (29.5 per cent) had a minimum titre of 1:20, with 30.14 per cent (41/136) of sheep and 28.07 per cent (16/57) of goats being seropositive. Tissue samples from all 193 animals, when subjected to B1 based PCR to detect T. gondii DNA, showed 3.67 per cent and 3.50 per cent to be positive in sheep and goats, respectively. In the present investigation B1 based PCR detected T. gondii in low numbers, possibly due to limitation of the sample size. The presence of T. gondii in tissues of sheep and goats slaughtered for human consumption in Chennai indicates the role of these food animals as potential sources of infection to human.
    Journal of parasitic diseases 09/2015; " (DOI: 10.1007/s12639-015-0713-x). DOI:10.1007/s12639-015-0713-x
  • Journal of parasitic diseases 06/2015; 39(2):309.
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    ABSTRACT: Epipenaeon ingens occurs most commonly in Southeast Coast of India parasitized the penaeid shrimp. The present study provides the prevalence, morphological and molecular characterisation of E. ingens collected from the host Metapenaeopsis stridulans. In the present study highlights the significance of high prevalence and genetic diversity of E. ingens in M. stridulans. Intra specific analysis also revealed that E. ingens species detected in the present study can be easily distinguished from the other genospecies of bopyrids. Further application of this molecular tool to investigate the genetic variability among bopyrids detected in different hosts may facilitate our understanding of the significance of genetic diversity in relation to the infestation of bopyrid species in south coast of India.
    Journal of parasitic diseases 03/2015; Online First(Online First):Online First. DOI:10.1007/s12639-015-0675-z
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    ABSTRACT: In the present study Ryukyua globosa parasitic isopod on the marine fishes of the Parangipettai coast are reported. R. globosa was found attached in the gill chamber of Sardinella gibbosa. The attachment position of Ryukyua species differs from that of other member of this genus. Until now, R. globosa was only reported on Amblygaster sirm and A.leiogaster. S.gibbosa is a new host record for R. globosa. R. globosa, branchial parasites, already known from Japan and is now recorded from the Southeast coast of India.
    Journal of parasitic diseases 03/2015; Online First(Online First):Online First. DOI:10.1007/s12639-015-0672-2
  • Journal of parasitic diseases 01/2015;
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    ABSTRACT: Two species of Cymothoidae (Nerocila loveni and Nerocila sundaica) were collected on the host fish Pomadasys maculatus from the Nagapattinam coast. Pomadasys maculatus is a new host for these parasitic isopods not previously recorded in the world. Nerocila loveni was mainly attached on the caudal peduncle of the fish and N. sundaica was collected from the pectoral fin and the body of the host. For N. loveni and N. sundaica, the prevalence reached 28.70 and 26.08 % respectively. One parasite was collected per host fish.
    Journal of parasitic diseases 11/2014; Online First. DOI:10.1007/s12639-014-0616-2
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    ABSTRACT: This study was conducted from August 2013 to January 2014. Host fishes were collected from the Nagapattinam Coast, India. During the sampling period, 242 fishes were infested out of 1440 specimens examined from nine different species of marine fishes. A total of 267 parasitic isopods belonging to nine cymothoid species viz., Anilocra dimidiata (Bleeker 1857), Catoessa boscii (Bleeker 1857), Cymothoa indica (Schioedte and Meinert 1884), Joryma sawayah (Bowman and Tareen 1983), Nerocila arres (Bowman and Tareen 1983), N.loveni (Bovallius 1887) N. phaiopleura (Bleeker 1857), N. serra (Schioedte and Meinert 1881) and N.sundaica (Bleeker 1857) were collected. The Nerocila species were attached to the pectoral fin, the caudal peduncle and different regions of the body surface of the host fishes. The specimen belonging to the species Catoessa boscii, Cymothoa indica and Joryma sawayah were collected from the mouth and the branchial cavity of the infected fishes. Anilocra dimidiata was only found on the body surface of the host fish. The overall prevalence reached 16.80 %. A maximum prevalence was observed in C. boscii parasitizing Carangoides malabaricus (26.81 %) and a minimum prevalence in N. sundaica parasitizing Terapon puta (P = 7.31 %). The mean intensity ranged from 1 to 1.3.
    Journal of parasitic diseases 11/2014; DOI:10.1007/s12639-014-0610-8