Asian Journal of Microbiology, Biotechnology and Environmental Sciences

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  • ISSN
    0972-3005

Publications in this journal

  • Asian Journal of Microbiology, Biotechnology and Environmental Sciences 09/2014;
  • Asian Journal of Microbiology, Biotechnology and Environmental Sciences 09/2014; 16(4):383-386.
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    ABSTRACT: Holoptelea integrifolia, a large spreading glabrous tree belonging to the family of ulmaceae is widely used in traditional medicine for treatment of cancer, gastritis, diabetes, wound healing and is known to possess many phytochemicals. The nature of phytochemicals present in the stem, leaf and roots were analyzed with different solvents using GC-MS analysis and evaluated for antimicrobial activity. The antimicrobial activity of these extracts were checked against a range of gram positive and gram negative pathogens using agar well diffusion method. The minimum inhibitory concentration and minimum bactericidal concentration was determined by two-fold broth dilution method and compared against the standard antibiotic chloramphenicol. The antimicrobial activities were comparable to those obtained with standard antibiotics. Root extracts had exhibited highest antimicrobial activity followed by stem and leaves. Acetone extracts of root and ethyl acetate extract of stem had exhibited good activity against the pathogens tested. The GC-MS analysis also revealed phenols, carboxylic acids, esters and other phytochemical components for Holoptelea integrifolia, which assured that it has high antimicrobial activity against pathogens. This study clearly demonstrates that Holoptelea integrifolia has good antimicrobial properties and the root has better antimicrobial properties than leaves and stem. All extracts except the aqueous extract had significant broad-spectrum inhibitory activity. Our study is the first to present the evidence of better antimicrobial properties of root over stem and leaves.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 03/2014; 16(1):145-154.
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    ABSTRACT: Meizotropis pellita (vern., Patwa) an endangered plant species found on the three sites in Patwadanger Nainital Uttarakhand. Meizotropis pellita is an angiospermic plant which belongs to family fabaceae. Most of the fabaceae family plants fix atmospheric nitrogen. Patwa belong to this family, so rhizobacterial spp. from its rhizospheric soil were isolated and characterized. This study focusses on the important role of Rhizobacteria associated with the Rhizospheric soil of Meizotropis pellita. By the biochemical properties of the Rhizobial isolates it is proved that these Rhizobial isolates have potent plant growth promoting abilities (PGPR). PGPR properties of Rhizobial isolates is based on their phosphate solubilisation property. All strain strain showed phosphate solubilisation property. Seven different rhizobacterial strains of bacteria were isolated from the rhizosphere of Patwa plant. Biochemical characterization was done for their identification, and molecular characterization authenticated that the isolated bacteria can fix atmospheric nitrogen and this may have PGPR activity. nif genes expresses and produce nitrogenase enzyme which is mainly responsible for nitrogen fixation. In this study we used PCR amplifications of nif genes present in the isolated bacterial strains. All seven isolate showed nif gene amplification. This is the first study associated with the Rhizospheric region of the M. pellita plant.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 16(2):301-306.
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    ABSTRACT: Abstract A Single bacterial colony was isolated by plating the aseptically separated crushed-cuticle sample of Penaeus monodon on an agar solidified LB medium, supplemented with 10 g/100 mL salt concentration. The bacterium was found to be gram positive, non-motile, aerobic and lactose non-fermenting through morphological and biochemical tests. The optimum culture condition of the isolate was at pH 7.5 with temperature of 300 C. The bacteria showed the ability of utilizing arabinose, sucrose, maltose, fructose as carbon sources and viable cell count was 489×107 CFU/mL. Isolated bacterium showed citrate metabolism ability, the ability of breaking down the amino acid tryptophan into indole and no catalase activity. The bacterium was resistant to amoxicillin, penicillin and vancomycin and the MIC value against penicillin was 100mg/mL. The bacterial colony was also specially characterized for CMCase production activity. The molecular weight of isolated bacterial genomic DNA was above 10 kb with not much smearing. High salt tolerance ability up to 12g/100mL medium was observed while culturing on different salt concentrations. Purpose of the study was to screen out a potential source of salt tolerant gene with CMCase activity.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 16(2):259-266.
  • Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 16(2):447-451.
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    ABSTRACT: Ethyl acetate and ethanolic extracts from the leaves of Justicia gendarusaLinn. (Acanthaceae) were investigated for their anthelmintic activity against adult Indian earthworm, Pheretima posthumaand nematode, Ascardia galli. Various concentrations (10, 20, 50 mg/mL) of each extract were tested in the bioassay, which involved determination of time of paralysis and time of death of the worms. Both the extracts exhibited significant anthelmintic activity at the highest concentration of 50 mg/mL. Piperazine citrate (15 mg/mL) and Albendazole (20 mg/mL) were used as standard references while 0.5 % carboxy methyl cellulose(CMC) in normal saline as control
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 15(4):725-728.
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    ABSTRACT: The present study aims to find out the efficiency of water hyacinth in accumulation and fast absorption of all heavy metals from the aqueous solution. This study infers that water hyacinth plays a vital role in phytoremediation. Eichhornia crassipes (water hyacinth) has been tested for removal of four important heavy metals chromium (Cr) lead (Pb) cadmium (Cd) and zinc (Zn) from metal solution. The plant was grown at four different 20 ppm metal solutions in laboratory condition. The result shows significant removal (upto 63%) of heavy metals during 10th day when compared to other different time point 1st day, 5th day, 8th day treated groups. Average physico chemical composition of the water was also analyzed at different time point 1st day, 5th day, 8th day and 10th day treated group showed a significant difference when compared to control and the 10th day treated group was highly significant. From our results we conclude Eichhornia crassipes has the efficiency to with stand all metal contamination of the selected heavy metals and it can be used for removal of heavy metals from metal polluted water bodies.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 09/2013; 16(1):115-120.
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    ABSTRACT: A bench scale batch fermentation study was carried out using the newly isolated lipase producing bacterial strains, Aneurinibacillus migulanus sp. SPSU B4, Pseudomonas sp. SPSU B3, and its mutant strain Pseudomonas sp. SPSU B3UVS30 using their respective optimal nutrient broth media containing emulsified oil and nitrogen sources. The fermentation process parameters were set as 30oC, 450 rpm, pH 8.0 for 24hr fermentation process. Broth samples (10mL) were withdrawn at an internal of 1hr from the respective broths separately and analyzed them for biomass produced and lipase activity, while the corresponding DO measurements were recorded from the control panel. In the results it was found that the fermentation of olive oil emulsified in the nutrient broth by Aneurinibacillu migulanus sp. SPSU B4, was of high metabolic rate producing the maximum growth associated lipase activity of 45U with its maximum growth of 3.9mg/mL by 12-14hrs of fermentation period. Similarly Pseudomonas sp. SPSU B3 produced the non-growth associated lipase to a maximum activity of 20U by selective and sequential consumption of available carbon sources (substrates) in the broth media, producing diauxic growth profile. On the other hand the mutant strain Pseudomonas sp. SPSU B3UVS30 produced the maximum biomass of 8.3mg/mL by 12hrs of fermentation period by simultaneous consumption of available substrates and produced mixed growth associated lipase to the extent of 40U. However, the DO levels in the fermentation broth of all the three strains, was found to be the limiting nutrient factor for the microbial growth and lipase production. So these novel lipase producing isolates Aneurinibacillu migulanus sp. SPSU B4, Pseudomonas sp. SPSU B3 and its mutant Pseudomonas sp. SPSU B3UVS30 may be preserved and made available to the scientific community for further exploration of the strains for their possible use in research, academics and industry.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 08/2013; 15(2):345-351.