Asian Journal of Microbiology, Biotechnology and Environmental Sciences


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    ABSTRACT: Endophytic bacteria are novel sources of beneficial compounds such as plant growth regulators and antibiotics. Nine bacterial isolates were obtained from sago palm tissue culture. Selected candidates were those colonies observed to have originated from the base of the explants. Eight of the isolates were Gram- negative bacilli (SEB 001, 002, 003, 004, 005, 006, 007, and 008) and one (SEB 206) was Gram-positive coccus. All eight isolates demonstrated the ability to ferment lactose and/ or sucrose, and were positive in catalase, citrate and VP tests. The isolates were negative for endospore formation, indole production, and methyl red test. Only one isolate grew in mannitol salt agar. Amplification of 16s rDNA sequences using the universal primers 16F27 and 16R1542 identified the isolates as Enterobacter ludwigii (SEBs 001, 003, and 006), Pantoea dispersa (SEBs 002 and 004), and Klebsiella variicola (SEBs 005, 007, and 008). Morphological and biochemical properties of the test isolates, as well as phylogenetic analysis, support the deduced identities. All isolates inhibited the growth of E. coli in the disc diffusion assays with SB 006 obtaining the highest mean zone of inhibition (12 mm). In the resazurin test, SEB 007 and SEB 008 showed promising antibiotic activities against E. coli, Aspergillus niger and Fusarium oxysporum. This study revealed that E. ludwigii and K. variicola isolated from sago palm possess antibiotic properties.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 12/2014; 16(4):777-786.
  • Asian Journal of Microbiology, Biotechnology and Environmental Sciences 09/2014;
  • Asian Journal of Microbiology, Biotechnology and Environmental Sciences 09/2014; 16(4):383-386.
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    ABSTRACT: Holoptelea integrifolia, a large spreading glabrous tree belonging to the family of ulmaceae is widely used in traditional medicine for treatment of cancer, gastritis, diabetes, wound healing and is known to possess many phytochemicals. The nature of phytochemicals present in the stem, leaf and roots were analyzed with different solvents using GC-MS analysis and evaluated for antimicrobial activity. The antimicrobial activity of these extracts were checked against a range of gram positive and gram negative pathogens using agar well diffusion method. The minimum inhibitory concentration and minimum bactericidal concentration was determined by two-fold broth dilution method and compared against the standard antibiotic chloramphenicol. The antimicrobial activities were comparable to those obtained with standard antibiotics. Root extracts had exhibited highest antimicrobial activity followed by stem and leaves. Acetone extracts of root and ethyl acetate extract of stem had exhibited good activity against the pathogens tested. The GC-MS analysis also revealed phenols, carboxylic acids, esters and other phytochemical components for Holoptelea integrifolia, which assured that it has high antimicrobial activity against pathogens. This study clearly demonstrates that Holoptelea integrifolia has good antimicrobial properties and the root has better antimicrobial properties than leaves and stem. All extracts except the aqueous extract had significant broad-spectrum inhibitory activity. Our study is the first to present the evidence of better antimicrobial properties of root over stem and leaves.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 03/2014; 16(1):145-154.
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    ABSTRACT: Abstract A Single bacterial colony was isolated by plating the aseptically separated crushed-cuticle sample of Penaeus monodon on an agar solidified LB medium, supplemented with 10 g/100 mL salt concentration. The bacterium was found to be gram positive, non-motile, aerobic and lactose non-fermenting through morphological and biochemical tests. The optimum culture condition of the isolate was at pH 7.5 with temperature of 300 C. The bacteria showed the ability of utilizing arabinose, sucrose, maltose, fructose as carbon sources and viable cell count was 489×107 CFU/mL. Isolated bacterium showed citrate metabolism ability, the ability of breaking down the amino acid tryptophan into indole and no catalase activity. The bacterium was resistant to amoxicillin, penicillin and vancomycin and the MIC value against penicillin was 100mg/mL. The bacterial colony was also specially characterized for CMCase production activity. The molecular weight of isolated bacterial genomic DNA was above 10 kb with not much smearing. High salt tolerance ability up to 12g/100mL medium was observed while culturing on different salt concentrations. Purpose of the study was to screen out a potential source of salt tolerant gene with CMCase activity.
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 16(2):259-266.
  • Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 16(2):447-451.
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    ABSTRACT: Ethyl acetate and ethanolic extracts from the leaves of Justicia gendarusaLinn. (Acanthaceae) were investigated for their anthelmintic activity against adult Indian earthworm, Pheretima posthumaand nematode, Ascardia galli. Various concentrations (10, 20, 50 mg/mL) of each extract were tested in the bioassay, which involved determination of time of paralysis and time of death of the worms. Both the extracts exhibited significant anthelmintic activity at the highest concentration of 50 mg/mL. Piperazine citrate (15 mg/mL) and Albendazole (20 mg/mL) were used as standard references while 0.5 % carboxy methyl cellulose(CMC) in normal saline as control
    Asian Journal of Microbiology, Biotechnology and Environmental Sciences 01/2014; 15(4):725-728.