World Journal of Microbiology and Biotechnology (WORLD J MICROB BIOT)

Publisher: Springer Verlag

Journal description

World Journal of Microbiology & Biotechnology publishes independently refereed research papers short communications technical communications and review articles on all aspects of applied microbiology and biotechnology including virology. The Journal seeks to provide a forum for research work directed towards microbiological and biotechnological solutions to global problems such as agriculture and food supplies and environmental issues including pollution waste management metal recovery bioleaching biological control agents etc. However it is recognized that many global issues for example improving crop productivity and public health have more acute consequences in the developing world than elsewhere. The Journal therefore aims to emphasize the role of biotechnological advances for and from the developing world whilst encouraging contributions from all scientists who have an interest in tackling these global problems. The editors also encourage contributions on aspects of education in microbiology and biotechnology and invite papers or reviews commenting on the social issues attendant with biotechnological applications. The Journal also publishes from time to time special review issues in which a topic of current interest is reviewed in depth by a group of invited scientists usually under the special editionship of a key leader in the area.

Current impact factor: 1.35

Impact Factor Rankings

2015 Impact Factor Available summer 2015
2013 / 2014 Impact Factor 1.353
2012 Impact Factor 1.262
2011 Impact Factor 1.532
2010 Impact Factor 1.214
2009 Impact Factor 1.082
2008 Impact Factor 0.945
2006 Impact Factor 0.471
2005 Impact Factor 0.634
2004 Impact Factor 0.478
2003 Impact Factor 0.516
2002 Impact Factor 0.498
2001 Impact Factor 0.445
2000 Impact Factor 0.538
1999 Impact Factor 0.57
1998 Impact Factor 0.598
1997 Impact Factor 0.746
1996 Impact Factor 0.608
1995 Impact Factor 0.483
1994 Impact Factor 0.382
1993 Impact Factor 0.226
1992 Impact Factor 0.385

Impact factor over time

Impact factor

Additional details

5-year impact 1.55
Cited half-life 6.00
Immediacy index 0.26
Eigenfactor 0.01
Article influence 0.36
Website World Journal of Microbiology and Biotechnology website
Other titles World journal of microbiology & biotechnology (Online), World journal of microbiology and biotechnology
ISSN 0959-3993
OCLC 37775874
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Springer Verlag

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    • Author's post-print on any open access repository after 12 months after publication
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    • Must link to publisher version
    • Set phrase to accompany link to published version (see policy)
    • Articles in some journals can be made Open Access on payment of additional charge
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: To investigate the temporal variation of the sediment prokaryote communities and their relation with the rapid increase of algae population in Taihu, a shallow eutrophic freshwater Lake, water and surface sediments were sampled from seven sites in different stages of algal bloom. The physicochemical characterization revealed positive correlations among the nutrient (N, P) parameters in the water and sediments, as well as TN/TP ratio 30.79 in average in water and 0.13 in sediments, demonstrating that P content was the limit factor for bloom in Taihu and sediment was an important nutrient resource for the water body. T-RFLP analysis of 16S rRNA genes revealed a diversity decrease of sediment prokaryotic communities along the bloom. The bacterial communities in sediments were more sensitive and shaped by the temporal changes, while archaea were more sensitive to the trophic level. The pyrosequencing data showed clear spatial and temporal changes in diversity of sediment bacteria. Betaproteobacteria was the most abundant group in all the samples, following by Delta-, Gama- and Alpha-proteobacteria, Acidobacteria, Chlorobi, Chloroflexi etc. At the genus level, Thiobacillus and Sulfuricurvum were the most dominant groups in the sediments, and the increase of Thiobacillus abundance in February might be used as bioindicator for the subsequent bloom. In addition, NO3 (-)-N was evidenced to be the main factor to regulate the bacterial community structure in the sediments. These results offered some novel and important data for the evaluation and predict the algal bloom in Taihu and can be reference for other shallow fresh water lakes.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1842-1
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    ABSTRACT: The water potential of rhizospheric soil is a key parameter that determines the availability of water, oxygen, and nutrients to plants and microbes. Recent global warming trends and erratic precipitation patterns have resulted in the emergence of drought as a major constraint of agricultural productivity. Though several strategies are being evaluated to address this issue, a novel approach is the utilization of microbes for alleviation of drought stress effects in crops. Citricoccus zhacaiensis B-4 is an osmotolerant actinobacterium isolated from banana rhizosphere on mannitol supplemented medium (-2.92 MPa osmotic potential). This isolate expressed plant growth promotion traits viz, IAA, GA3 production, phosphate, zinc solubilization, ACC deaminase activity and ammonia production under PEG induced osmotic stress and non-stress conditions. Under in vitro osmotic conditions, biopriming with the actinobacterium improved the percent germination, seedling vigour and germination rate of onion seeds (cv. Arka Kalyan) at osmotic potentials up to -0.8 MPa. Considering its novelty, osmotolerance and plant growth promoting traits, biopriming with C. zhacaiensis is suggested as a viable option for the promotion of onion seed germination under drought stressed environments.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1837-y
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    ABSTRACT: Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. Such demands can be fulfilled by a brief method which enables confirmation of strain identity. This communication describes one step identification method for two common Trichoderma species; T. citrinoviride and T. reesei, based on identification of polymorphic region in the nucleotide sequence of translation elongation factor 1 alpha. A unique forward primer and common reverse primer resulted in 153 and 139 bp amplicon for T. citrinoviride and T. reesei, respectively. Simplification was further introduced by using mycelium as template for PCR amplification. Method described in this communication allows rapid, one step identification of two Trichoderma species.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1839-9
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    ABSTRACT: Melamine belongs to the s-triazine family, and industrially used as raw product in many ways all over the world. Melamine has been reported for human harmful effects and detected from some crops, soil and water. To remove melamine from the polluted environment, the efficient melamine-mineralizing microorganisms have been needed. We newly isolated three melamine-degrading bacteria from the same upland soil sample using soil-charcoal perfusion method. These bacteria were classified as Arthrobacter sp. MCO, Arthrobacter sp. CSP and Microbacterium sp. ZEL by 16S rRNA genes sequencing analysis. Both Arthrobacter species completely degraded melamine within 2 days, and consumed melamine as a sole nitrogen source. Both strains also grew in cyanuric acid as sole nitrogen source, and released small quantities of ammonium ions. These strains are the first identified bacteria that can mineralize both melamine and cyanuric acid as sole initial nitrogen source in Arthrobacter sp. Although ammeline and ammelide intermediates were detected, these strains possess none of the known genes encoding melamine degrading enzymes. Since the Arthrobacter strains also degraded melamine in a high pH liquid medium, they present as potential bioremediation agents in melamine-polluted environments.
    World Journal of Microbiology and Biotechnology 03/2015; 31(5). DOI:10.1007/s11274-015-1832-3
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    ABSTRACT: A new strain of Bacillus coagulans CGMCC 9551, which has a broad range of antibacterial activities against six main pathogenic bacteria including Escherichia coli O8, Staphylococcus aureus, Salmonella enterica subsp. enterica serovar enteritidis, Streptococcus suis, Listeria monocytogenes and Pasteurella multocida, was isolated from healthy piglet feces. In adhesion assay, the isolate exhibited a stronger adhesion to pig intestinal mucus than that of B. subtilis JT143 and L. acidophilus LY24 respectively isolated from BioPlus(®)2B and FloraFIT(®) Probiotics (P < 0.05). The adhesion activity reached 44.5 ± 3.2, 48.9 ± 2.6, 42.6 ± 3.3 and 37.6 ± 2.4 % to jejunum, ileum, transverse colon and sigmoid colon, separately. The survival rate of B. coagulans CGMCC 9551 was reduced by only 20 % at 4 h exposure under 0.9 % w/v bile salt. The strain was fully resistant to pH 2 for 2 h with 90.1 ± 3.5 % survival and susceptible to 15 antibiotics commonly used in veterinary medicine. Additionally, the bacteria showed amylase, protease and cellulase activities. The safety assessment demonstrated the lack of toxicity potential in B. coagulans CGMCC 9551 by ligated rabbit ileal loop assay, acute and subchronic toxicity test. These results implied that that the new strain of B. coagulans CGMCC 9951 isolated from healthy piglet feces has promising probiotic characteristics and offers desirable opportunities for its successful commercialization as one excellent candidate probiotic.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1838-x
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    ABSTRACT: The cereal-pathogenic Fusarium culmorum (W.G. Smith), causal agent of various blights and rot diseases, is considered as a chronic fungus of economic concern worldwide including North African countries such as Algeria. This pathogen produces a wide range of mycotoxins, amongst which the type B-trichothecene deoxynivalenol (DON). In addition to its acute and chronic side effects in livestock and humans, DON is believed to play a determinant role in the pathogenesis toward Triticeae. However, regardless its significant occurrence and impact, little is known about trichothecenes-producing ability of F. culmorum infecting cereals in Algeria. The PCR assay based on Tri genes of 12 F. culmorum strains (designated Fc1-Fc12), which were recovered from several cropping areas of North Algeria, revealed their trichothecenes-producing ability with 3-AcDON genotype. The molecular prediction was confirmed by HPLC analysis. All strains were able to produce the toxin at detectable levels. Strains Fc1 and Fc12 were the highest producers of this mycotoxin with 220 and 230 µg g(-1), respectively. The evaluation of pathogenic ability of strains through a barley infesting experiment exhibited the significant disease impact of most strains. Significant correlation between the DON-producing ability of strains and the increase in both disease severity (r = 0.88, P = 0.05) and disease occurrence (r = 0.70, P = 0.05) was observed. Chemotyping of F. culmorum isolates and evaluation of their pathogenic ability are reported for the first time for isolates from Algeria, and highlights the important potential of F. culmorum to contaminate cultivated cereal with DON trichothecenes.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1841-2
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    ABSTRACT: The richness, phylogeny and composition of archaeal community associated with the roots of common reed (Phragmites australis) growing in the Beijing Cuihu Wetland, China was investigated using a 16S rDNA library. In total, 235 individual sequences were collected, and a phylogenetic analysis revealed that 69.4 and 11.5 % of clones were affiliated with the Euryarchaeota and the Crenarchaeota, respectively. In Euryarchaeota, the archaeal community was dominated by species in following genera: Methanobacterium in the order Methanobacteriales (60.7 %); Methanoregula and Methanospirillum in the order Methanomicrobiales (20.2 %), and Methanomethylovorans, Methanosarcina and Methanosaeta in the order Methanosarcinales (17.2 %). Of 27 sequences assigned to uncultured Crenarchaeota, 22 were grouped into Group 1.3, and five grouped into Group 1.1b. Hence, the archaeal communities associated with reed roots are largely involved in methane production, and, to a lesser extent, in ammonia oxidization. Quantification of the archaeal amoA gene indicated that ammonia oxidizing archaea were more numerous in the rhizosphere soil than in the root tissue or surrounding water. A total of 19.1 % of the sequences were unclassified, suggesting that many unidentified archaea are probably involved in the reed wetland ecosystem.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1836-z
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    ABSTRACT: 16S rRNA Crenarchaeota and Thermoplasmata sequences retrieved from 22 anaerobic digesters were analysed. 4.8 and 0.53 % of archaeal sequences were simultaneously affiliated to these lineages. A core of 2 operational taxonomic units (OTUs) representing 0.6 to -33.6 % of all archaeal sequences were defined for the Crenarchaeotes and identified to already known but not yet cultivable organisms in almost half of the digesters sampled. For the Thermoplasmata, apparently less abundant with 0.7 to -4.7 % of the archaeal sequences, 3 OTUs were identified. We showed here that Crenarchaeotes coexist with methanogens and are particularly abundant when Arch I lineage (also called WSA2 by Hugenholtz) is dominant in digesters. Moreover, Thermoplasmata were detected when Crenarchaeota were present. Interactions between methanogens, Crenarchaeotea and Thermoplamata were thus discussed.
    World Journal of Microbiology and Biotechnology 03/2015; DOI:10.1007/s11274-015-1834-1
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    ABSTRACT: Nitrogen causes the frequent occurrence of harmful algal blooms and possible microcystin production. The effects of ammonia and alanine (Ala) on the growth and microcystin production of Microcystis aeruginosa were investigated using an isotope tracer ((15)N). The results indicated that Ala was directly used by M. aeruginosa and contributed to biomass formation amounting to 2.1 × 10(7) cells mL(-1) on day 48, compared with only 6.2 × 10(6) cells mL(-1) from ammonia alone. Microcystin-LR production with Ala was less than that of ammonia, which peaked at 50.2 fg cell(-1) on day 6. Liquid chromatographic analysis with tandem mass spectrometry of (15)N-microcystin-LR suggested that (15)N from ammonia was probably synthesized into the arginine residue. By contrast, (15)N from Ala was assimilated into the Ala, leucine, the iso-linked (2R,3S)-3-methylaspartic acid, arginine, and certain unusual C20 amino acid residues. The results represent the forward steps in the determination of the nitrogen forms that fuel toxin production and blooms.
    World Journal of Microbiology and Biotechnology 03/2015; 31(5). DOI:10.1007/s11274-015-1829-y
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    ABSTRACT: Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum.
    World Journal of Microbiology and Biotechnology 02/2015; 31(4). DOI:10.1007/s11274-014-1781-2
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    ABSTRACT: A structured heterotrophic-iron (II) induction (HII) strategy was proposed to enhance lipid accumulation in oleaginous Chlorella protothecoides. C. protothecoides subjected to heterotrophic-iron (II) induction achieved a favorable lipid accumulation up to 62 % and a maximum lipid productivity of 820.17 mg/day, representing 2.78-fold and 3.64-fold increase respectively over heterotrophic cultivation alone. HII-induced cells produced significantly elevated levels of 16:0, 18:1(Δ9), and 18:2(Δ9,12) fatty acids (over 90 %). The lipid contents and plant lipid-like fatty acid compositions exhibit the potential of HII-induced C. protothecoides as biodiesel feedstock. Furthermore, 31 altered proteins in HII-induced algal cells were successfully identified. These differentially expressed proteins were assigned into nine molecular function categories, including carbohydrate metabolism, lipid biosynthesis, Calvin cycle, cellular respiration, photosynthesis, energy and transport, protein biosynthesis, regulate and defense, and unclassified. Analysis using the Kyoto encyclopedia of genes and genomes and gene ontology annotation showed that malic enzyme, acyltransferase, and ACP were key metabolic checkpoints found to modulate lipid accumulation in C. protothecoides. The results provided possible applications of HII cultivation strategy in other microalgal species and new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity.
    World Journal of Microbiology and Biotechnology 02/2015; 31(5). DOI:10.1007/s11274-015-1831-4
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    ABSTRACT: The purposes of this study were to isolate and evaluate the interaction between mineral-weathering bacteria and silicate minerals (feldspar and biotite). A mineral-weathering bacterium was isolated from weathered rocks and identified as Rhizobium tropici Q34 based on 16S rRNA gene sequence analysis. Si and K concentrations were increased by 1.3- to 4.0-fold and 1.1- to 1.7-fold in the live bacterium-inoculated cultures compared with the controls respectively. Significant increases in the productions of tartaric and succinic acids and extracellular polysaccharides by strain Q34 were observed in cultures with minerals. Furthermore, significantly more tartaric acid and polysaccharide productions by strain Q34 were obtained in the presence of feldspar, while better growth and more citric acid production of strain Q34 were observed in the presence of biotite. Mineral dissolution experiments showed that the organic acids and polysaccharides produced by strain Q34 were also capable of promoting the release of Si and K from the minerals. The results showed that the growth and metabolite production of strain Q34 were enhanced in the presence of the minerals and different mineral exerted distinct impacts on the growth and metabolite production. The bio-weathering process is probably a synergistic action of organic acids and extracellular polysaccharides produced by the bacterium.
    World Journal of Microbiology and Biotechnology 02/2015; 31(5). DOI:10.1007/s11274-015-1827-0
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    ABSTRACT: The microbial community composition in three soil fractions (bulk soil, rhizosphere and rhizoplane) of the root-soil system of a thistle, Cirsium arvense, and of a tufted hair grass, Deschampsia caespitosa, was investigated. The two spontaneous wild plant species were predominant in two Italian lands contaminated since centuries by arsenic and at present show high levels of arsenic (from 215 to 12,500 mg kg(-1)). In order to better understand how the rhizobacterial ecosystem responds to a long-term arsenic contamination in term of composition and functioning, culture-independent techniques (DAPI counts, fluorescence in situ hybridization and denaturing gradient gel electrophoresis analysis) along with cultivation-based methods were applied. Microbial community structure was qualitatively similar in the two root-soil systems, but some quantitative differences were observed. Bacteria of the α-, β-, and γ-subclasses of the Proteobacteria were dominant in all fractions, while the subdominant groups (Cytophagaceae, gram-positive spore-forming, and filamentous bacteria) were significantly more abundant in the root-soil system of D. caespitosa. As regards to arsenic resistant strains, Firmicutes, Actinobacteria, Enterobacteria and γ-Proteobacteria were isolated from soil system of both plants. Our results suggest that the response to a high level of arsenic contamination governed the rhizosphere microbial community structure together with the soil structure and the plant host type effects. Data from this study can provide better understanding of complex bacterial communities in metal-polluted soils, as well as useful information of indigenous bacterial strains with potential application to soil remediation.
    World Journal of Microbiology and Biotechnology 02/2015; DOI:10.1007/s11274-015-1826-1
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    ABSTRACT: Pichia anomala (Wickerhamomyces anomalus) WRL-076 was discovered by a visual screening bioassay for its antagonism against Aspergillus flavus. The yeast was shown to significantly inhibit aflatoxin production and the growth of A. flavus. P. anomala is a potential biocontrol agent for reduction of aflatoxin in the food chain. Maintaining the viability of biocontrol agents in formulated products is a great challenge for commercial applications. Four media, NYG, NYGS, NYGT and NYGST are described which support good growth of yeast cells and were tested as storage formulations. Post growth supplement of 5 % trehalose to NYGST resulted in 83 % viable yeast cells after 12 months in cold storage. Intracellular sorbitol and trehalose concentrations were determined by HPLC analysis at the beginning of the storage and at the end of 12 month. Correlation of cell viability to both trehalose and sorbitol suggested a synergistic effect. Bonferroni (Dunn) t Test, Tukey's Studentized Range (HSD) Test and Duncan's Multiple Range Test, all showed that yeast cell viability in samples with both intracellular trehalose and sorbitol were significantly higher than those with either or none, at a 95 % confidence level. DiBAC4(5) and CFDA-AM were used as the membrane integrity fluorescent stains to create a two-color vital staining scheme with red and green fluorescence, respectively. Yeast cells stored in formulations NYG and NYGS with no detectable trehalose, displayed mostly red fluorescence. Yeast cells in NYGST+5T showed mostly green fluorescence.
    World Journal of Microbiology and Biotechnology 02/2015; 31(5). DOI:10.1007/s11274-015-1824-3
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    ABSTRACT: The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III PKS (SoRppA) gene and its adjacent gene for cytochrome P450 158A2 (SoCYP158A2) of this gene cluster were cloned by PCR screening through a fosmid genomic library of S. olivaceus FXJ 7.023. Tandem expression of SoRppA and SoCYP158A2 in Escherichia coli strain BL21 (DE3) plysS resulted in obvious biosynthesis of phenol and indole, while heterologous expression of SoRppA or SoCYP158A2 alone did not. The engineered strain sorppAcyp158a2BL21 showed tolerance to phenol concentration up to 0.75 g/L. Continuous biosynthesis of phenol and indole by the immobilized engineered strain on macroporousresin was achieved, and the productivities of phenol and indole in extractant-free culture in 102 h were 0.08 and 1.525 g/L/h, respectively, with the highest production reached 0.67 and 14.48 g/L, respectively. These results suggest that the engineered strain and immobilized continuous fermentation process may provide potential for "green" production of phenol and indole.
    World Journal of Microbiology and Biotechnology 02/2015; 31(4). DOI:10.1007/s11274-015-1825-2
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    ABSTRACT: Haloferax mediterranei has potential for economical industrial-scale production of polyhydroxyalkanoate (PHA) as it can utilize cheap carbon sources, has capacity for nonsterile cultivation and allows simple product recovery. Molasses-based Indian distilleries are converting themselves to cereal-based distilleries. Waste stillage (14 l) of rice-based ethanol industry was used for the production of PHA by H. mediterranei in the simple plug-flow reactor configuration of the activated sludge process. Cells utilized stillage and accumulated 63 ± 3 % PHA of dry cell weight and produced 13.12 ± 0.05 g PHA/l. The product yield coefficient was 0.27 while 0.14 g/l h volumetric productivity was reached. Simultaneous lowering of 5-day biochemical oxygen demand and chemical oxygen demand values of stillage by 82 % was attained. The biopolymer was characterized as poly-3-(hydroxybutyrate-co-17.9 mol%-hydroxyvalerate) (PHBV). Directional properties of decanoic acid jointly with temperature-dependent water solubility in decanoic acid were employed for two-step desalination of the spent stillage medium in a cylindrical baffled-tank with an immersed heater and a stirrer holding axial and radial impellers. 99.3 % of the medium salts were recovered and re-used for PHA production. The cost of PHBV was estimated as US$2.05/kg when the annual production was simulated as 1890 tons. Desalination contributed maximally to the overall cost. Technology and cost-analysis demonstrate that PHA production integrated with ethanol manufacture is feasible in India. This study could be the basis for construction of a pilot plant.
    World Journal of Microbiology and Biotechnology 02/2015; DOI:10.1007/s11274-015-1823-4
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    ABSTRACT: A strain of marine bacterium Bacillus firmus YBf-10 with nematicidal activity was originally isolated by our group. In the present study, the systemic nematicidal activity and biocontrol efficacy in pot experiment of B. firmus YBf-10 were investigated. Our results showed that YBf-10 exhibits systemic nematicidal activity against Meloidogyne incognita, including lethal activity, inhibition of egg hatch and motility. Pot experiment suggested that soil drenching with YBf-10 efficiently reduced damage of M. incognita to tomato plants, such as reduction of galls, egg masses on roots, and final nematode population in soil; and moreover, YBf-10 significantly promoted host plant growth. In addition, our results also indicated that the systemic nematicidal activity is likely attributed to the secondary metabolites produced by YBf-10. The obtained results of the current study confirmed that B. firmus YBf-10 is a promising nematicidal agent, and has great potential in plant-parasitic nematicidal management.
    World Journal of Microbiology and Biotechnology 02/2015; 31(4). DOI:10.1007/s11274-015-1820-7
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    ABSTRACT: There was no direct correlation in substrate specificity between the metabolism of Pseudomonas stutzeri DEH138 and its corresponding dehalogenase. Dehalogenase substrates that could be dehalogenated might not be degraded by DEH138 or vice versa. Basing on this, different approaches to enhance L-2-haloacid dehalogenase (L-DEX) production in DEH138 via the combination of non-halogenated compounds with different inducers were applied. The optimum approach to obtain more L-DEX from DEH138 was the combination of DL-lactate and DL-2-chlorobutyrate, with 5.7-fold greater production and 11.7-fold greater productivity of the enzyme after optimization.
    World Journal of Microbiology and Biotechnology 02/2015; 31(4). DOI:10.1007/s11274-015-1817-2