Biosensors and Bioelectronics (BIOSENS BIOELECTRON)

Publications in this journal

• Article: Nanotechnology in glucose monitoring: Advances and challenges in the last 10 years

  Viviana Scognamiglio
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  ABSTRACT: In the last decades, a wide multitude of research activity has been focused on the development of biosensors for glucose monitoring, devoted to overcome the challenges associated with smart analytical performances with commercial implications. Crucial issues still nowadays elude biosensors to enter the market, such as sensitivity, stability, miniaturisation, continuous and in situ monitoring in a complex matrix. A noteworthy tendency of biosensor technology is likely to push towards nanotechnology, which allows to reduce dimensions at the nanoscale, consenting the construction of arrays for high throughput analysis with the integration of microfluidics, and enhancing the performance of the biological components by using new nanomaterials. This review aims to highlight current trends in biosensors for glucose monitoring based on nanotechnology, reporting widespread representative examples of the recent approaches for nanobiosensors over the past 10 years. Progress in nanotechnology for the development of biosensing systems for blood glucose level monitoring will be discussed, in view of their design and construction on the bases of the new materials offered by nanotechnology.
  Biosensors and Bioelectronics 09/2013; 47.
• Article: Bio-Microfluidic platform for gold nanoprobe based DNA detection – application to Mycobacterium tuberculosis

  Iwona Bernacka-Wojcik, Paulo Lopes, Ana Catarina Vaz, Bruno Veigas, Pawel Jerzy Wojcik, Pedro Simões, David Barata, Elvira Fortunato, Pedro Viana Baptista, Hugo Águas, Rodrigo Martins
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  ABSTRACT: We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high- aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 μl on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
  Biosensors and Bioelectronics 03/2013;
• Article: LSI-based amperometric sensor for real-time monitoring of embryoid bodies

  Mustafa Şen, Kosuke Ino, Kumi Y. Inoue, Toshiharu Arai, Taku Nishijo, Ryota Kunikata, Atsushi Suda, Hitoshi Shiku, Tomokazu Matsue
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  ABSTRACT: A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. Real-time monitoring of the EB ALP activity for 3.5 h indicated decrease in the ALP activity after 2 h in a glucose-free buffer solution. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3 h. The chip is expected to be useful for the evaluation of 3D tissues.
  Biosensors and Bioelectronics 03/2013;
• Article: Mutual enhancement of the current density and the coulombic efficiency for a bioanode by entrapping bi-enzymes with Os-complex modified electrodeposition paints

  Minling Shao, Muhammad Nadeem Zafar, Christoph Sygmund, Dmitrii A. Guschin, Roland Ludwig, Clemens K. Peterbauer, Wolfgang Schuhmann, Lo Gorton
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  ABSTRACT: A bioanode with high current density and coulombic efficiency was developed by co-immobilization of pyranose dehydrogenase from Agaricus meleagris (AmPDH) with the dehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophiles (recDHCtCDH) expressed recombinantly in Escherichia coli. The two enzymes were entrapped in Os-complex modified electrodeposition polymers (Os-EDPs) with specifically adapted redox potential by means of chemical co-deposition. AmPDH oxidizes glucose at both the C2 and C3 positions whereas recDHCtCDH oxidizes glucose only at the C1 position. Electrochemical measurements reveal that maximally 6 electrons can be harvested from one glucose molecule at the two-enzyme anode via a cascade reaction, as AmPDH oxidizes the products formed from of the recDHCtCDH catalyzed substrate oxidation and vice versa. Furthermore, a significant increase in current density can be obtained by combining AmPDH and recDHCtCDH in a single modified electrode. We propose the use of this bioanode in biofuel cells with increased current density and coulombic efficiency. Keywords Cellobiose dehydrogenase (CDH); Coulombic efficiency; Pyranose dehydrogenase (PDH); Os-complex modified polymer; Redox polymer; Electron transfer
  Biosensors and Bioelectronics 02/2013; 40(1):308-314.
• Article: Heart type fatty acid binding protein:Structure,function and biosensing applications for early detection of myocardial infarction

  Ankana Kakoti, PranabGoswami
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  ABSTRACT: Heart type fatty acid binding protein(HFABP) as an early marker of cardiac injury holds a promising future with studies indicating surpassing performance as compared to myoglobin. As a plasma marker, this cytoplasmic protein owing to its small size( �15 kDa) and water solubility, appears readily in the blood-stream following cardiomyocyte damage,reachingpeaklevelswithin6hofsymptomonset.Low plasma levelsofHFABPascomparedtotissuelevelsindicatethatminuteamountsoftheproteinwhen releasedduringmyocardialinfarctionleadstoagreaterproportionalrise.Theseparametersofkinetic releasemakeitanidealcandidateforrapidassessmentofacutemyocardialinfarction(AMI).Theneed for developmentofrapidimmunoassaysandimmunotestssoastouseHFABPasanearlymarkerfor AMI exclusionistremendous.Inthepresentreview,weoutlinethevariousimmunoassaysand immunosensorsdevelopedsofarforthedetectionofHFABPinbuffer,plasmaorwholeblood.The principlesbehindthedetectiontechniquesalongwiththeirperformanceparameterscomparedto standardELISAtechniquesareelucidated.
  Biosensors and Bioelectronics 01/2013; 43:400-411.
• Article: Registration of the protein with compact disk

  Ivanov YD, Pleshakova TO, Krohin NV, Kaysheva AL, Usanov SA, Archakov AI
  Biosensors and Bioelectronics 01/2013;
• Article: Specific DNA detection using antibody mediated fluorescence quenching.

  Frank Sellrie, Elmara Graser, Christine Lenz, Timo Hillebrand, Jörg A.Schenk
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  ABSTRACT: First homogenous immunoassay for sequence-specific nucleic acid detection is developed. The assay bases on our finding that a fluorophore inserted into a DNA probe instead of one of the internal nucleotides may get protected from fluorescence quenching caused by an anti-fluorophore antibody, if the probe is hybridized with the target sequence. This ensures a positive signal in the antibody presence. The assay enables quantitative detection and may have potential for development of homogenous high-throughput platforms.
  Biosensors and Bioelectronics 01/2013; 42:512-515.
• Article: Smart Plastic Antibody Material (SPAM) tailored on disposable screen printed electrodes for protein recognition: application to Myoglobin detection

  Felismina T.C. Moreira, Sanjiv Sharma, Rosa A.F. Dutra, Anthony E.G. Cass, João P.C. Noronha, M.Goreti F. Sales
  Biosensors and Bioelectronics 01/2013;
• Article: High-speed integrated optical logic based on the protein bacteriorhodopsin

  A. Mathesz, L. Fábián, S. Valkai, D. Alexandre, P. V. S. Marques, P. Ormos, E. K. Wolff, A. Dér
  Biosensors and Bioelectronics 01/2013; 46:48.
• Article: Gold surface supported spherical liposome–gold nano-particle nano-composite for label free DNAsensing

  M. Bhuvana a, J.ShankaraNarayanan a, V.Dharuman a, W.Teng b, J.H.Hahn b, K.Jayakumar a
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  ABSTRACT: Immobilization of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) liposome–gold nanoparticle (DOPE–AuNP) nano-composite covalently on 3-mercaptopropionic acid (MPA) on gold surface is demonstrated for the first time for electrochemical label free DNA sensing. Spherical nature of the DOPE on the MPA monolayer is confirmed by the appearance of sigmoidal voltammetric profile, characteristic behavior of linear diffusion, for the MPA–DOPE in presence of [Fe(CN)6]3�/4� and [Ru(NH3)6]3þ redox probes. The DOPE liposome vesicle fusion is prevented by electroless deposition of AuNP on the hydrophilic amine head groups of the DOPE. Immobilization of single stranded DNA (ssDNA) is made via simple gold–thiol linkage for DNA hybridization sensing in the presence of [Fe(CN)6]3�/4�. The sensor discriminates the hybridized (complementary target hybridized), unhybridized (non-complementary target hybridized) and single base mismatch target hybridized surfaces sensitively and selectively without signal amplification. The lowest target DNA concentration detected is 0.1�10�12 M. Cyclic voltammetry (CV), electrochemical impedance (EIS), differential pulse voltammetry (DPV) and quartz crystal microbalance (QCM) techniques are used for DNA sensing on DOPE–AuNP nano-composite. Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS) and Ultraviolet– Visible (UV) spectroscopic techniques are used to understand the interactions between the DOPE, AuNP and ssDNA. The results indicate the presence of an intact and well defined spherical DOPE–AuNP nanocomposite on the gold surface. The method could be applied for fabrication of the surface based liposome–AuNP–DNA composite for cell transfection studies at reduced reagents and costs.
  Biosensors and Bioelectronics 10/2012; 41:802.
• Article: Chitosan encapsulated quantum dots platform for leukemia detection. Sharma A, Pandey CM, Chatterjee T, Malhotra BD, etal. Biosensors Bioelectron 2012 Oct 38(1)-107-13

  Sharma A, Pabdey CM, Chatterjee T, Malhotra BD
  Biosensors and Bioelectronics 10/2012;
• Article: Fabricationofhydrogenperoxidebiosensorbasedon Ni dopedSnO2 nanoparticles

  N. Lavanya, S. Radhakrishnan, C. Sekar
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  ABSTRACT: Ni dopedSnO2 nanoparticles(0–5wt%)havebeenpreparedbyasimplemicrowaveirradiation (2.45 GHz)method.PowderX-raydiffraction(XRD)andtransmissionelectronmicroscopy(TEM) studiesconfirmedtheformationofrutilestructurewithspacegroup(P42/mnm) andnanocrystalline natureoftheproductswithsphericalmorphology.Directelectrochemistryofhorseradishperoxidase (HRP)/nano-SnO2 compositehasbeenstudied.Theimmobilizedenzymeretaineditsbioactivity, exhibitedasurfaceconfined,reversibleone-protonandone-electrontransferreaction,andhadgood stability,activityandafastheterogeneouselectrontransferrate.Asignificantenzymeloading (3.374�10�10 mol cm�2) hasbeenobtainedonnano-NidopedSnO2 as comparedtothebareglassy carbon(GC)andnano-SnO2 modifiedsurfaces.ThisHRP/nano-Ni–SnO2 film hasbeenusedforsensitive detectionofH2O2 by differentialpulsevoltammetry(DPV),whichexhibitedawiderlinearityrange from 1.0�10�7 to 3.0�10�4 M (R¼0.9897)withadetectionlimitof43nM.TheapparentMichaelis– Mentenconstant(KM app) ofHRPonthenano-Ni–SnO2 was estimatedas0.221mM.Thisexcellent performanceofthefabricatedbiosensorisattributedtolargesurface-to-volumeratioandNidoping into SnO2 whichfacilitatethedirectelectrontransferbetweentheredoxenzymeandthesurfaceof electrode
  Biosensors and Bioelectronics 04/2012; 36(2012):41-47.
• Article: Wang, J.; Zhang, P.; Li, C. M.; Li, Y. F.; Huang, C. Z., A Highly Selective and Colorimetric Assay of Lysine by Molecular-Driven Gold Nanorods Assembly. Biosens. Bioelectron. 2012, 34 (1), 197-201.

  Wang, Zhang, Li, C. M, Y. F, Huang, C. Z
  Biosensors and Bioelectronics 02/2012; 34(1-2):197-201.