European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies (Eur J Clin Chem Clin Biochem)

Publications in this journal

• Article: Clinical laboratory sciences versus laboratory medicine.

  X Fuentes-Arderiu
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):939-40.
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  Available from: nbn-resolving.de

  Article: Determination of HbA1c in the presence of haemoglobin variants: comparison of three HPLC techniques.

  M Carta, G Dall'Olio, G Soffiati
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  ABSTRACT: The presence of Hb variants may cause analytical interference in HbA1c values measured with the HPLC techniques currently used in Italian laboratories. In this study performance of a new HPLC system, (HA-8140, Menarini) was compared with two other traditional HPLC systems (HA-8121, Menarini and Diamat, Bio-Rad). The HA-8140 system detects the Hb variant possibly present in an independent peak. The integration area of such a peak is not computed in the calculation of the percentage value of HbA1c. In this manner the underestimation of values obtained with traditional HPLC systems is avoided.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):923-5.
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  Available from: nbn-resolving.de

  Article: The optimized use of gas chromatography-mass spectrometry and high performance liquid chromatography to analyse the serum bile acids of patients with metabolic cholestasis and peroxisomal disorders.

  F Courillon, M F Gerhardt, A Myara, F Rocchiccioli, F Trivin
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  ABSTRACT: We have measured the bile acids in human serum as methyl ester-trimethylsilyl ethers by gas chromatography-mass spectrometry (GC-MS) using an electron ionization procedure. The overall method was validated and the detection limit (0.4 mumol/l), linearity (2-30 mumol/l), intra-day and inter-day precision, accuracy and recovery (96.2% for nor-23-deoxycholic acid as internal standard) were measured. Serum C24-bile acids profiles from 43 cholestatic patients were measured by GC-MS and by HPLC. The results obtained with the two methods were well correlated and the criteria for selecting either HPLC or GC-MS identified. The serum C24- and C27-bile acids and C29 dicarboxylic bile acid profiles for patients with generalized peroxisomal deficiencies, like Zellweger syndrome (n = 5), neonatal adrenoleukodystrophy (n = 1), infantile Refsum disease (n = 2) and from a single peroxisomal deficiency (n = 1) were also measured by GC-MS.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):919-22.
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  Available from: nbn-resolving.de

  Article: The origins of the European Confederation of Laboratory Medicine (ECLM)

  R Dybkaer
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):937.
• Article: It is rational to measure serum lactate dehydrogenase isoenzyme-1 activity in patients with testicular germ cell tumours.

  F E von Eyben, P Hyltoft Petersen, O Blaabjerg, E Lindegaard Madsen
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):943.
• Article: "Ocean-to-Ocean Project": a transcontinental external quality assessment trial in clinical chemistry realized throughout Eurasia.

  J Kratochvíla, M Budina, H Baadenhuijsen, A Moshkin, E Legenstein, E Kaiser, B Friedecký, P Schneiderka, A Lapin
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  ABSTRACT: More than 800 diagnostic laboratories situated throughout the Eur-Asian continent--from the Pacific Coast up to the North Sea littoral--were involved in a common survey of External Quality Assessment (EQA). It consisted of the simultaneous measurement of up to 30 analytes of 'general' clinical chemistry using the same batch of control material. The laboratories were associated in four EQA institutions: SKZL (The Netherlands), OQUASTA (Austria), SEKK (Czech Republic) and BKKSystem (Community of Independent States). The results demonstrated the feasibility of such a large-scale survey and provided a realistic idea about the state-of-the-art of laboratory diagnosis in these countries: Besides some local specific problems, such as poor quality of water or the forced use of reagents and calibrators from different sources, there are general problems hindering an efficient process of 'harmonization' in laboratory medicine, namely, the high methodological dispersion especially in the case of enzymes and of some organic analytes. At the same time there is a potential necessity for more concentrated implementation of internal quality assessment into the routine work of laboratories.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):927-35.
• Article: More on inter-method variability of sodium and potassium measurements in patients sera and control materials.

  F Zoppi, E Guagnellini, A Manzoni
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):941-2.
• Article: Award of the Sarstedt Research Prize 1997.

  C Wagener, F Körber
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):945-6.
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  Available from: nbn-resolving.de

  Article: Concentration of the cross-linked carboxyterminal telopeptide of type I collagen in serum of young growing rats fed a low calcium and vitamin D-deficient diet.

  A R Bielaczyc, M Gołebiewska, A Citko, F Rogowski
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  ABSTRACT: Type I collagen is the main type found in mineralized bone. Specific radioimmunoassay for the cross-linked carboxyterminal telopeptide of type I collagen allows assessing the degradation of type I collagen in serum samples. The aim of the present investigation was to determine the concentration of cross-linked carboxyterminal telopeptide of type I collagen in serum of dietary calcium and vitamin D-deficient rats, a good model disease of decreased formation and mineralization of bone matrix and excessive bone resorption. The studies were carried out on 20 young growing Wistar rats. Serum concentration of the cross-linked carboxyterminal telopeptide of type I collagen was analyzed by the Rat Telopeptide [125I]ICTP Radioimmunoassay Kit obtained from Orion Diagnostica (Finland). The data obtained from biochemical analysis showed increased concentration of the cross-linked carboxyterminal telopeptide of type I collagen in the serum of rats fed a low calcium and vitamin D-deficient diet after 14 days of the experiment. At the end of the experiment (day 21), the concentration of carboxyterminal telopeptide of type I collagen in serum was still elevated in these animals. In conclusion, dietary calcium and vitamin D-deficiency in rats produces hypocalcaemia together with the increased concentration of the cross-linked carboxyterminal telopeptide of type I collagen in serum.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 01/1998; 35(12):915-8.
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  Available from: hu-berlin.de

  Article: Determination of plasma lipid hydroperoxides by an NADPH/NADP+ coupled enzyme reaction system. Evaluation of a method.

  C Ruiz, A Alegría, R Barberá, R Farré, M J Lagarda
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  ABSTRACT: Several techniques based on different principles have been proposed to measure lipid hydroperoxides. Enzymatic methods are sensitive and can be quite specific but they are subject to interference by inhibitors and not all are stoichiometric. The present work proposes some modifications of the Heath & Tappel (Anal Biochem 1976; 7:184-91) enzymatic method of determination of lipid hydroperoxides in order to standardize and automate it and to meet the analytical criteria required for a biological assay. The proposed new protocol and the automated assay give acceptable within-run and between-run precisions, with coefficients of variation of 3.34% and 5.80%, respectively, at the usual plasma lipid hydroperoxides content. The recovery is 98.7 +/- 4.89%, and the method is linear for a wide range of contents and sensitive (10 mumol/l) enough to measure the plasma lipid hydroperoxides content.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 12/1997; 35(12):893-8.
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  Available from: hu-berlin.de

  Article: Intra- and inter-individual biological variability data bank.

  M A Sebastián-Gámbaro, F J Lirón-Hernández, X Fuentes-Arderiu
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  ABSTRACT: Different results are usually observed when a quantity is measured in different specimens from the same individual obtained over a time span. For an individual, this variation is due to the imprecision of the measurement procedure, that is to say the metrological variability, as well as to the rhythmic and random fluctuations of the quantity value around a virtual homeostatic set point, that is to say the intra-individual biological variability. On the other hand, when studying the intra-individual biological variation of a quantity a mean value, the virtual homeostatic set point, is estimated for each individual participating in the study. The variation among these mean values is due to the inter-individual biological variability.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 12/1997; 35(11):845-52.
• Article: The first fully automated allergy analyser UniCAP: comparison with IMMULITE for allergy panel testing.

  G M Costongs, B M Bas
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  ABSTRACT: Automated immunoassay systems should be convenient to handle, flexible and give reliable results. To investigate the extent to which the UniCAP System met the above requirements, compared with the IMMULITE System, we compared the Phadiatop (UniCAP) and AlaTOP (IMMULITE) results of 110 patients with positive clinical diagnoses for inhalant allergy. In addition, we compared food screening test results of 103 patients with a clinical positive diagnosis for food, and 110 test results of controls with negative diagnosis for allergy. Phadiatop had a sensitivity of 96% and a specificity of 92%. AlaTOP had a sensitivity of 86% and a specificity of 94%. For food screening the results were: 75% sensitivity and 82% specificity for fx5 (UniCAP) and 63% sensitivity and 71% specificity for fp5 (IMMULITE). Furthermore, those samples for which the test results which were not in concordance with the clinical diagnosis were tested with the follow-up panel of the different screening tests. For the AlaTOP follow-up we had to use the DPC microplate System (Milenia), because single allergen testing is not yet possible on the IMMULITE System. With regard to sensitivity, the UniCAP specific inhalant allergen tests and the original Phadiatop results showed closer agreement with each other than did the Milenia specific allergen results with the AlaTOP. The specificity of the single inhalant allergen tests was the same for both systems. For food allergy testing the UniCAP System shows closer agreement between the screening and the follow-up results than does the IMMULITE. The hands on time for loading 44 samples was practically the same for both systems, but for the follow-up tests the Milenia System is used next to the IMMULITE. Therefore from a logistical point of view the UniCAP System is more convenient. From these results we conclude that both logistically and clinically UniCAP seems to meet our requirements better than the IMMULITE.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 12/1997; 35(11):885-8.
• Article: Reference intervals for serum thyrotropin, free thyroxine and free triiodothyronine in healthy adults in Finland, measured by an immunoautomate based on time-resolved fluorescence (AutoDELFIA).

  E Taimela, V Kairisto, P Koskinen, A Leino, K Irjala
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  ABSTRACT: We have established reference intervals for healthy adults of serum thyrotropin, free thyroxine and free triiodothyronine using the AutoDELFIA (Wallac, Finland) automatic measuring device. The determination of reference intervals in a proper manner is costly, and many laboratories adopt reference ranges from the literature rather than determining them alone. This is the first report on reference values in thyroidology where this automatic system based on time-resolved fluorescence has been used. The reference intervals for thyrotropin, free thyroxine and free triiodothyronine were 0.6-4.3 mIU/l, 9.6-17.1 pmol/l and 4.3-7.5 pmol/l, respectively.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 12/1997; 35(11):889-90.
• Article: Effect of an encapsulated anti-elastase compound on experimental gingival inflammation in the rat.

  F Guessous, A el Abbouyi, J P Giroud, J Meyer, M Roch-Arveiller
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  ABSTRACT: An animal (rat) model of gingival injury ("impaction") induced a gingival inflammatory reaction, which was characterized by a breakdown of gingival collagen and the elastic network, as well as a significant increase of gingival elastase. The present study was conducted to investigate whether ceramides, sphingolipids composed of sphingosine N-acyl-linked to fatty acids, a chemical structure with antielastase properties, could counteract the development of such an inflammatory process. The ceramides used in these experimental series were extracted from wheat and characterized. The main fatty acids were 16:0, 18:1, 18:2, and the sphingoid moiety was phytosphingosine. Inhibition of elastase by ceramides was demonstrated in vitro and the concentration necessary to inhibit 50% of elastase activity was 41 mg/l using the synthetic substrate methoxysuccinyl-alanine-alanine-proline-valine-p-nitroanilide (MeOSuc-AlaAlaProValpNA). However, this anti-elastase activity was not observed in vivo in our animal model of gingival inflammation. A glycosaminoglycan (Heparin), recognized as a potent inhibitor of elastase, was entrapped in ceramides. A local treatment of impacted gingivae by encapsulated heparin led to a dose-related decrease of the elastase level in gingival extracts. Encapsulation in ceramides potentiated the effect exerted by heparin alone. This inhibitory effect of encapsulated heparin on elastase suggested a vector effect of these amphipathic molecules.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 12/1997; 35(11):867-71.
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  Available from: nbn-resolving.de

  Article: Detection of the three Kunitz-type single domains of membrane-bound tissue factor pathway inhibitor (TFPI) by flow cytometry.

  C Tiemann, T Brinkmann, K Kleesiek
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  ABSTRACT: Tissue factor pathway inhibitor, a natural anticoagulant in the extrinsic pathway of blood coagulation, is associated with the endothelial membrane and presumed to be released by heparin. For flow cytometric detection of membrane-bound tissue factor pathway inhibitor we synthesized polyclonal monospecific antibodies directed against each of the three Kunitz-type domains. Antisera were obtained by immunisation of rabbits with synthetic oligopeptides representing the reactive site of each domain. Kunitz-domain delta 1: 26CAFKDDGPCKAIMKR41, domain delta 2: 101EDPGICRGYITR112 and domain delta 3: 192PADRGLCRANENR204. Different cell lines (chondrosarcoma, synovial sarcoma, synovial cells, leukaemic monocytes) and endothelial cells were investigated by flow cytometric analysis using these antibodies. The three tissue factor pathway inhibitor domains were detected on the surface of all cells by the corresponding antisera. Similar results were obtained by immuno-histochemical staining. Since domain delta 3 was recognised by the appropriate antibody, it would seem that this third domain is not the membrane binding site. To investigate the cellular tissue factor pathway inhibitor release, endothelial cells were cultivated with heparin. Protein resynthesis and translocation were inhibited by puromycin and monensin, respectively. After heparin incubation an increased tissue factor pathway inhibitor concentration was determined in the cell culture medium by a chromogenic substrate assay. However, the tissue factor pathway inhibitor density on the cell surface was not influenced by heparin, as shown by flow cytometry using the three tissue factor pathway inhibitor antisera. Our results suggest that functionally active tissue factor pathway inhibitor is not released from the cell surface. Therefore, the effect of heparin appears to be mediated by secretion of tissue factor pathway inhibitor from intracellular stores.
  European journal of clinical chemistry and clinical biochemistry: journal of the Forum of European Clinical Chemistry Societies 12/1997; 35(11):855-60.