JPC - Journal of Planar Chromatography - Modern TLC (JPC-J PLANAR CHROMAT)

Publisher: Akadémiai Kiadó

Journal description

Journal of Planar Chromatography- Modern TLC is an international journal devoted exclusively to the publication of research papers on analytical and preparative planar chromatography. The journal covers all fields of planar chromatogeaphy, on all kinds of stationary phases (paper, layer, gel) and with various modes of migration of the mobile phase (capillary action of forced flow).

Current impact factor: 0.67

Impact Factor Rankings

2015 Impact Factor Available summer 2016
2013 / 2014 Impact Factor 0.67
2012 Impact Factor 0.955
2011 Impact Factor 0.767
2010 Impact Factor 1.247
2009 Impact Factor 0.662
2008 Impact Factor 0.982
2006 Impact Factor 1.153
2005 Impact Factor 0.667
2004 Impact Factor 0.824
2003 Impact Factor 0.879
2002 Impact Factor 1.047
2001 Impact Factor 0.555
2000 Impact Factor 1.118
1999 Impact Factor 1.03
1998 Impact Factor 1.321
1997 Impact Factor 0.643
1996 Impact Factor 1.096
1995 Impact Factor 0.759
1994 Impact Factor 1.394

Impact factor over time

Impact factor

Additional details

5-year impact 0.79
Cited half-life 6.90
Immediacy index 0.16
Eigenfactor 0.00
Article influence 0.19
Other titles JPC
ISSN 0933-4173
OCLC 160109042
Material type Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Akadémiai Kiadó

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Authors own final version only can be archived
    • Publisher's version/PDF cannot be used
    • On author's personal website or institutional repository or any repository mandated by Author's funding body
    • Published source must be acknowledged
    • Must state that the file is not the final published version of the paper
    • Must link to publisher version([DOI of the Article without brackets])
    • Articles in some journals can be made Open Access on payment of additional charge
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Imidacloprid is a member of chloronicotinyl neonicotinoid compounds. It is the most important systemic insecticide and has a wide diversity of uses: in agriculture, on turfs, on pets, and for household pests. During the last decade, Forensic Science Laboratories of Maharashtra State, India, detected a large number of human poisoning cases with imidacloprid. Since a large number of biological samples were received for toxicological analysis, thin-layer chromatography (TLC) was the method of choice. This study reports that cobalt thiocyanate was found to be a selective and sensitive spray reagent for imidacloprid in routine analysis by TLC. Imidacloprid reacts with cobalt thiocyanate which gives intense blue-colored compound. The cobalt thiocyanate reagent does not react with the organochlorine insecticides, organophosphorus insecticides, and pyrethroids. Visceral constituents (amino acids, peptides, proteins, etc.) do not interfere.
    JPC - Journal of Planar Chromatography - Modern TLC 10/2015; 28(5):352-353. DOI:10.1556/1006.2015.28.5.2
  • [Show abstract] [Hide abstract]
    ABSTRACT: Despite the fact that many researchers have developed different methods for the determination and detection of bile acid sequestrant, the analytical instruments are still quite costly, and expertise is usually required. In order to avoid the above-mentioned problems, simple, rapid, effective, and inexpensive methods to evaluate bile acid-binding are essential. The purpose of this study was to develop a simple and rapid thin-layer chromatography (TLC)-image analysis method to quantitatively determine the bile acid-binding activity of ten edible ethanolic plant extracts. The method was carried out by using TLC silica gel 60 F254 plate with the mobile phase composed of chloroform, methanol, and acetic acid (7:2:1 v/v). The chromatograms were analyzed with image analysis software (ImageJ). The method provided good linearity, accuracy, reproducibility, and selectivity for the determination of bile acid-binding activity. Litsea petiolata extract was found to show the most potent bile acid-binding activity compared to other extracts. Moreover, some extracts exhibited selective binding ability to bile acid; for example, extracts of Careya sphaerica and Polygonum odoratum could selectively bind rather to taurocholic acid than glycodeoxycholic acid. This study shows a simple method for the evaluation of potential edible plants to be used as cholesterol-reducing agents due to their bile acid-binding activity.
    JPC - Journal of Planar Chromatography - Modern TLC 10/2015; 28(5):380-385. DOI:10.1556/1006.2015.28.5.6
  • [Show abstract] [Hide abstract]
    ABSTRACT: Dyslipidemia, which may be manifested by elevation of the total cholesterol concentration in the blood, is one of the most common medical conditions that affect individuals. This work presents a simple, fast, and precise high-performance thin-layer chromatography (HPTLC) technique for the multicomponent analysis of rosuvastatin (Ros) and fenofibrate (Fen), Ros and ezetimibe (Eze), and Ros and aspirin (Asp) in three different pharmaceutical combos prescribed for the treatment of dyslipidemia. Techniques were performed on silica gel 60 F254 TLC precoated aluminum plates with a simple mobile phase combination of toluene-acetone-acetic acid in the ratio of 6:2:0.2 v/v and produced well-resolved compact spot for all titled drugs. The linear regression statistics has shown a good linear relationship over a concentration range of 500-4000 ng spot1 for all cited drugs with a good correlation coefficient of 0.9999, and densitometric detection was carried out in the absorbance mode at 254 nm. The sensitivity of the developed technique was assessed in terms of the limit of quantification and the limit of detection. The stability study disclosed that all four drugs were stable at room temperature up to 12 h. The cited drugs were validated as per International Conference on Harmonization (ICH) guidelines. The technique was found to be specific and selective since no extra peak was recorded alongside with the main peak. The robustness study, recovery study, and the percentage of the assay of the formulations were found within the limit as per ICH guidelines. Marketable tablets were also analyzed for five different brands by the developed methodology with no interference detected from the excipients in the formulations. The cost-effectiveness, sensitivity, and selectivity of the proposed technique recommend its applicability for the routine quality-control analysis of any of the named drugs in their pharmaceutical preparations.
    JPC - Journal of Planar Chromatography - Modern TLC 10/2015; 28(5):354-361. DOI:10.1556/1006.2015.28.5.3
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new spectrodensitometric method was developed for the simultaneous determination of a quaternary mixture of antihyperlipidemics containing niacin, atorvastatin, and bezafibrate with either ezetimibe or simvastatin. Densitometric analysis was carried out using high-performance thin-layer chromatographic (HPTLC) silica gel 60G F254 plates as the stationary phase. The plates were developed with benzene-ACN-n-butanol (7:2:1, v/v) + 1.50%, v/v, glacial HOAC in absorbance mode at 242 nm. The retention factors of niacin, atorvastatin, bezafibrate, ezetimibe, and simvastatin were 0.17, 0.38, 0.51, 0.65, and 0.66, respectively. The method was validated according to the United States Pharmacopeia and National Formulary (USP 31-NF 26) and the International Conference on Harmonization (ICH) guidelines. Linearity ranges of all studied drugs were found to be in the range of 15-650 ng band1 with correlation coefficient values of 0.9975 or more. Limits of detection and quantitation were 5-50 and 15-150 ng band1, respectively. Upon applying polynomial regression to the same concentration ranges of standard solutions of all investigated drugs as well as spiked bezafibrate samples to rabbit plasma, correlation coefficient values had greatly improved. The proposed method was successfully applied for the simultaneous determination of the studied antihyperlipidemic drugs in plasma and in their pharmaceutical formulations. The developed method was utilized to study the pharmacokinetic behavior of bezafibrate and its drug-drug interaction with atorvastatin in rabbit males. This study has proven the increased myotoxicity risk upon coadministration of atorvastatin with bezafibrate.
    JPC - Journal of Planar Chromatography - Modern TLC 10/2015; 28(5):362-372. DOI:10.1556/1006.2015.28.5.4
  • [Show abstract] [Hide abstract]
    ABSTRACT: An efficient and fast microwave-Assisted extraction (MAE) technique was developed for extracting trigonelline as an indicative biomarker for the quality control of Trigonella foenum-graecum seeds. The MAE procedure was optimized and compared with other conventional extraction techniques. The optimal conditions of MAE were 50% methanol as solvent, solid-liquid ratio 1:20 g mL1, irradiation power 40%, and two extraction cycles, 3 min each. The proposed extraction technique produced a maximum yield of 7.89 mg g1 trigonelline in 6 min which was 1.28 and 2.20 times more efficient than 3 h of heat reflux and 15 h of maceration extraction, respectively. Furthermore, rapid high-performance thin-layer chromatographic and high-performance liquid chromatographic methods for the quantification of trigonelline were established and validated. The methods were found to be simple, sensitive, precise, accurate, and specific for the estimation of trigonelline in T. foenum-graecum seeds extract and overcame disadvantages of previously published methods.
    JPC - Journal of Planar Chromatography - Modern TLC 10/2015; 28(5):373-379. DOI:10.1556/1006.2015.28.5.5

  • JPC - Journal of Planar Chromatography - Modern TLC 10/2015; 28(5):391-394. DOI:10.1556/1006.2015.28.5.8
  • [Show abstract] [Hide abstract]
    ABSTRACT: Simple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)-densitometric methods were developed for the simultaneous determination of carbinoxamine (CAR), pholcodine (PHL), and ephedrine (EPH) in antitussive syrup. In method A, reversed-phase (RP)-HPLC analysis was performed on an Inertsil CN-3 column (250 mm × 4.6 mm, 5 μm), using a mobile phase consisting of acetonitrile-distilled water (pH 3.5) using orthophosphoric acid in the ratio 70:30 (v/v) and flow rate of 1.5 mL min-1. Quantitation was achieved with ultraviolet (UV) detection at 220 nm. In method B, TLC analysis was carried out on an aluminum-backed sheet of silica gel 60 F254 layer using chloroform- propanol-ammonia (6:4:0.1, v/v) as the mobile. Quantification was carried out with UV detection at 245 nm. The validation of the proposed methods was applied according to the International Conference on Harmonization (ICH) guidelines. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial dosage form.
    JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):307-315. DOI:10.1556/1006.2015.28.4.7
  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple, rapid, and effective high-performance thin-layer chromatographic method has been developed for the analysis and quantitative determination of ellagic acid, gallic acid, and methyl gallate in the galls of Quercus infectoria Olivier. Analysis was performed on silica gel G F254 TLC plates with toluene-ethyl acetate-formic acid (6:4.5:2, volume ratio) as the mobile phase. Densitometric detection was performed at 300 nm. The method was validated for linearity, precision, stability, recovery, and robustness. The limits of detection (LOD) and quantification (LOQ) were determined. The calibration curves showed a good linearity for ellagic acid, gallic acid, and methyl gallate with correlation coefficients of 0.9995, 0.9997, and 0.9996, respectively. The LOD and LOQ values were in the nanogram range for all three compounds. The average recovery was >96%. The amounts of ellagic acid, gallic acid, and methyl gallate in the galls of Q. infectoria were determined by using calibration graphs. The developed method is simple, with good precision, stability, recovery, and robustness. This method can satisfy the requirements for quantitative determination and provides a new and effective way for the quality monitoring and evaluation of galls of Q. infectoria.
    JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):300-306. DOI:10.1556/1006.2015.28.4.6
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of astragaloside IV and formononetin in Radix Astragali. Samples were employed to degrease the materials by petroleum ether (boiling point: 60-90C) and extracted by methanol, and then were alkalized and extracted with n-butanol saturated with water. Separation was achieved on HPTLC plates using petroleum ether (boiling point: 60-90C) and n-butanol saturated with water-glacial acetic acid as the mobile phase, the results of which were compared with HPLC. The well-resolved peaks for astragaloside IV and formononetin were observed at Rf values 0.43 ± 0.02 and 0.75 ± 0.02, respectively. The calibration curves were found linear with a wide range of concentration 1.01-10.10 μg μL-1 with good correlation coefficient for astragaloside IV and formononetin. The method was validated for linearity, precision, reproducibility, accuracy, and limits of detection and quantification. This simple, rapid, sensitive, economic, and reliable HPTLC method is suitable for the routine quantitative analysis and quality control of traditional Chinese medicines (TCMs) such as Radix Astragali, which can be applied for the quality control of saponins and flavonoids in other plants or extracts.
    JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):268-273. DOI:10.1556/1006.2015.28.4.1

  • JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):333-336. DOI:10.1556/1006.2015.28.4.10
  • [Show abstract] [Hide abstract]
    ABSTRACT: Context: Bacoside A, a triterpenoid saponin, is a major constituent isolated from Bacopa monnieri (L.) Wettst. (Scrophulariaceae), used as a memory enhancer. Bacoside A and B are active ingredients in Bacopa herb and have antioxidant and hepatoprotective activities. Objective: A new rapid, simple, and economical high-performance thin-layer chromatographic (HPTLC) method was developed and validated for densitometric quantitative analysis of bacoside A in powdered leaves from different geographical regions of India. Materials and methods: An amount of 10 mg mL-1 methanol extract of powdered leaves from different geographic regions was used for sample application on precoated silica gel 60 F254 aluminum sheets. Standard bacoside A (1 mg mL-1) was used for calibration curve. HPTLC separation was performed on percolated silica gel aluminum plate 60 F254 (20 cm × 10 cm with 0.2 mm thickness) as a stationary phase using ethyl acetate-methanol-water (4:1:1) as the mobile phase. Quantification was achieved by densitometric analysis at 598 nm over the concentration range of 500-4000 ng band-1. Result: Compact and well-resolved bands for bacoside A from powdered leaves of different geographic regions were found at retardation factor (Rf) 0.53 ± 0.02. The linear regression analysis data for calibration curve showed good linear relationship with regression coefficient r2 = 0.9996 and r2 = 0.99810 with respect to peak area and peak height. The method was validated for precision, recovery, and robustness as per the International Conference on Harmonization (ICH) guidelines. Variation in quantitative analysis of bacoside A in powdered leaves sample from different geographic regions was found by HPTLC method. Discussion and conclusion: The highest and lowest content of bacoside A in powdered leaves sample from Jammu and Kerala regions, respectively. The variety of B. monnieri in Jammu is superior to other regions of India. The proposed developed HPTLC method can be applied for the quantitative determination of bacoside A in powdered leaves of plant and its formulation.
    JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):287-293. DOI:10.1556/1006.2015.28.4.4
  • [Show abstract] [Hide abstract]
    ABSTRACT: The isolation and characterization of bioactive compounds from medicinal plants is usually a significant challenge in phytochemical analysis because of the natural chemical complexity of plant extracts. However, there exists a need for analytical tools which can quantitatively separate and characterize the components from these biosources with greater chromatographic selectivity and lesser analytical run times that facilitate the evaluation with enhanced separation profiles. Hyphenation of thin-layer chromatography (TLC/HPTLC) with mass spectrometry (MS) is an alternative for screening herbal extracts because of its rapid analysis and ability to aid structural characterization with powerful analytical capacity. The aim of the present study was to develop a sophisticated analytical method which utilizes HPTLC-MS coupling for the chromatographic profiling and evaluation of the therapeutically important genus Piper (Piperaceae). In this study, six marker compounds, namely, trichostachine, piperine, 4,5-dihydropiperlonguminine, guineensine, pellitorine, and sesamin were analyzed and quantified in extracts of Piper nigrum L. and compared with those of Piper longum L. and Piper chaba Hunter. All the samples tested showed similar phytochemical profiles, but the contents of the active ingredients varied. Additionally, HPTLC-MS further allowed confirming the identification of the constituents in the analyzed samples with greater chromatographic selectivity where HPTLC facilitated a selective chromatographic resolution, while MS offered an efficient characterization of the target compounds in one analytical run. The study finds a potential utility in adopting HPTLC-MS as a rapid and high throughput method for the efficient quantification and identification of marker compounds from medicinal plants.
    JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):280-286. DOI:10.1556/1006.2015.28.4.3
  • [Show abstract] [Hide abstract]
    ABSTRACT: A validated, sensitive, and highly selective stability-indicating high-performance thin-layer chromatographic (HPTLC) method has been adopted for the quantitative determination of pyridostigmine bromide in the presence of its alkaline-induced degradation product and in pharmaceutical formulations. 3-hydroxy-N-methyl pyridinium bromide (3-OH NMP) is the metabolite, impurity, and alkaline-induced degradation product of pyridostigmine bromide (PB). Pyridostigmine bromide and its alkaline-induced degradation product were separated on silica gel HPTLC F254 plates using methanol–ethyl acetate–triethyl amine–glacial acetic acid (9:1:0.5:0.05 by volume) as the developing system followed by scanning of the separated bands at 270 nm over a concentration range of 2–10 μg band−1 with mean percentage recoveries of 99.84% (SD 1.384). The proposed method was successfully applied to the analysis of pyridostigmine bromide both in bulk powder and in pharmaceutical formulation without interference from other dosage form additives. The results obtained by the proposed method were statistically compared with those obtained by the reported HPLC method with no significant difference regarding both accuracy and precision, indicating the ability of the proposed method to be reliable and suitable for routine analysis of a drug product.
    JPC - Journal of Planar Chromatography - Modern TLC 08/2015; 28(4):316–322. DOI:10.1556/1006.2015.28.4.8