Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk (Fiziol Z Im M Sechenova )

Publisher: Rossijskaja Akademija Nauk


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    Sechenov physiological journal, Fiziologic̆eskij z̆urnal
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Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: In pathogenesis of the traumatic disease of the spinal cord, two mechanisms of the injuries of its neuronal apparatus are defined: primary (necrosis) and secondary (apoptosis). In the work a participation of a number of internal causes in the progression of apoptosis in injury of the spinal cord and peripheral nerve trunks, the role of those remains little-studied up to date, is discussed.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 02/2014; 100(2):145-60.
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    ABSTRACT: The run of trained rats until exhaustion affected changes of red-ox balance in red blood cells (RBC) and deteriorated the acid stability of RBC. The oral administration of quercetin, 20 mg/kg, caused expansion of oxidative stress in RBC and a significant decrease in RBC acid stability. Pro-oxidant activity of quercetin could contribute accelerated intravascular hemolysis. It was accompanied by a significant reticulocytosis compared with a control group of rats no fed on antioxidant but exposed to run. Green tea extract (GTE) oral administration, 12 mg/kg, resulted in preventing oxidative stress in RBC, increasing in acid stability of RBC and reducing reticulocytosis. Thus, prooxidant activity of quercetin and the high antioxidant efficacy of GTE are indicative of the advantages of the complex preparations containing several antioxidant compounds. Such complex preparations may counteract an oxidative stress and save mechanical stability of erythrocytes under exhausted run.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1223-32.
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    ABSTRACT: White rat males (Wistar) were cooled in the water (9-10 °C) to the stop breathing (at rectal temperature 14.7 ± 0.5°, brain 16.0 ± 0.3 °C). After the removal of water animals were injected 0.5% solution disodium salt ethylenediaminetetraacetic acid (Na2EDTA) intravenously, which reduced the concentration of Ca2+ in the blood. Breathing rats were resumed after 4-8 min after administration Na2EDTA. Then one group of rats was cooled to the temperature of the body 12.2 ± 0.7 (brain 14.9 ± 0.3 °C). At such a low temperatures the breath of rats was long-term (2-3 hours) supported at the level of 12 ± 3 cycle/min. The second group of rats was not additionally cooled after the resumption of breathing. The same dose of Na2EDTA caused the rise of the respiratory rate to a higher level--29 ± 4 cycles/min at a temperature in the rectum and the brain 15-16°. Apparently, the process of accumulation of Ca2+ in the cytoplasm of the respiratory center's cells was slowed after the Na2EDTA injection to hypothermal rats in the bloodstream. It is assumed that the Na2EDTA injection under the hypothermia conditions was activated the cell's protection mechanisms from cold damage. The obtained results and the literature data allowed to conclude that nonhibernators are able to maintain basic life functions under significant reduction in the body temperature.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1214-22.
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    ABSTRACT: The research was carried out in 60 days old posterity of female rats which suffer chronic alcohol-induced liver injury. In this work we studied motivational element of sexual behavior under conditions of "barrier" experiment. Intensity of sexual motivational behavior was estimated by the change of activity near the barrier, under which there was a receptive oestrous female. The appearance of sexual activity with the animals under experiment was estimated in the experiment of direct interaction of sexual partners. It was found, that "alcoholic" animals have the symptoms of malfunction of sexual motivation formation. The experiment shows that males which had mothers with chronic alcohol-induced hepatobiliary system injury have the deviations of sexual behavior display. These deviations show the weak form of sexual behavior induction of "alcoholic" males that can be the result of nervous system disorders.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1181-90.
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    ABSTRACT: In the present work we have studied the effects of p53 on the proliferation and differentiation of neural progenitor cells (NPC) in mouse hippocampal organotypic culture. To study the role of p53 the selective p53 inhibitor pifithrin-alpha (PFT) and activator tenovin 1 (TEN) were used in the experiments. Obtained data demonstrated that the injections of PFT did not affect on the amount of phospho-H3 positive cells in the subgranular zone of hippocampus. This data revealed that p53 inhibition does not change the proliferation level of the NPC. In opposite, at the TEN treatments we observed increased of the proliferation activity. Analysis of Pim-1 and Phb 1, which regulate cell cycle progression, demonstrated that p53 activation led to increased level of Pim-1 as well as the proliferation. Thus, our data correlate with published ones and proposed that Pim-1 positively regulates NPC cell cycle progression. In opposite to Pim-1, Phb 1 has anti-proliferative action. Our obtained data demonstrated that TEN diminished Phb 1 expression. Primarily PFT injections led to the increasing Phbl level, but then dramatically decreased it that accompanied with unchanged proliferation level. In other words, increased proliferation level after TEN treatments, which we observed, can be partly depend from the inhibition of anti-proliferative activity of Phb. In our study we demonstrated that both TEN and in a greater degree PFT stimulates neuronal differentiation by activation of CRMP-2 expression, but do not affect on gliogenesis. Thus, obtained data revealed that p53 is an important factor of neuronal differentiation and, probably, p53 action is mediated by cell cycle regulator protein such as Pim-1 and Phbl.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1160-74.
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    ABSTRACT: The effect of insulin on contractility of directly stimulated skeletal muscles was studied in experiments in isolated preparations of rat fast, extensor digitorum longus (m. EDL), slow, soleus (m. SOL) and mixed, diaphragm muscles. In addition (diaphragm only) characteristics of extracellularly recorded muscle fiber action potentials (APs) were evaluated before and after addition of insulin to a bath solution. Insulin (0.5-10 nM) decreased muscle twitch force. This negative inotropic effect of insulin was dose-dependent, with m. SOL appearing to be more sensitive to insulin than either m. EDL or diaphragm. Insulin did not affect strength of isotonic KCL- or caffeine-induced muscle contractures, but decreased second and increased first and third phases of extracellularly recorded muscle fiber APs. The analysis of the data obtained in this study and the data from the literature suggests changes in electrogenesis of the muscle fiber's t-tubular plasma membrane as a key element of the negative inotropic effect of the hormone on contractility of mammalian skeletal musculature. Possible mechanisms of such putative changes are discussed.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1200-13.
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    ABSTRACT: Features of the existential organization of a bioelectric activity of a brain at posterity from rats with a lateral injury of the brain were investigated. The EEG analysis revealed in one month animals significantly increased the proportion of advanced and lagging decrease in the activity of the right frontal derivation towards the right occipital region. Change between structural ties in terms of the rats were correlated with the severity of behavioral disorders in the test "open field" (OF). The most significant change in the existential organization of a bioelectrical activity (the reduction of synchronous communications between the frontal derivations) recorded in rats with severe conduct disorder compared with animals of the same groups, in which the behavior was not significantly different from the norm. The violation of the behavior in the test OF was accompanied by a decrease in synchronous activity between the left frontal and right occipital region or the decline in the anticipatory activity in the right frontal region in relation to the right occipital region. By maintaining the integrity of the behavior of these parameters were increased in comparison with an intact control.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1149-59.
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    ABSTRACT: The work is dedicated to the study of the influence of separate rhythmogenic areas on spontaneous activity of the remained areas in the non-pregnant rat myometrium. The main characteristics of pacemaker activity (spike frequency, spike amplitude, duration of burst discharges, summarized period of the active state) of each active region were analyzed under the conditions blocking the influence of others. Parameters of the ovarian horn area did not undergo significant changes unlike the cervical horn area and uterine corpus. The greatest suppression of spike activity parameters was shown in the uterine corpus. It was also revealed that certain activity characteristics (the amplitude of the spikes, duration of burst discharges, summarized period of the active state) are more subjected to change than other parameters. It is concluded that the ovarian horn area may have a controlling role in the interaction of asynchronous pacemaker activities of the rhythmogenic areas in the uterus.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1191-9.
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    ABSTRACT: The inhabitancy in Far North increases a probability of development of coronary heart disease, acute myocardial infarction and arterial hypertension. Catecholamines and NO deficiency play a substantial role in the development of cold hypertension, but these are not involved in acclimatization cardiac hypertrophy. Data of in vivo experiments indicate in favor of an involvement of α-adrenergic receptors (ARs) in the mechanism of an appearance of cold hypertension. Cold acclimatization promotes an elevation of β3-AR density and decrease in β1-AR and β2-AR quantity on sarcolemma of cardiomyocytes. Experimental data indicate the important role of aldosterone and angiotensin II in the development of acclimatization hypertension. Catecholamines, aldosterone and angiotensin II are not involved in cold hypertrophy of heart. Experimental data demonstrate the important role of endothelin ETA receptor in the formation of cold hypertrophy of heart and cardiofibrosis. Thyroid hormones play substantial role in the development of cold hypertension and cardiac hypertrophy.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1113-24.
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    ABSTRACT: Recently, it was shown that pacemaker f/HCN channel might be permeable not only for Na+ and K+, but also for calcium ions. Pacemaker channel HCN2 was expressed in ovarian cell culture of Chinese hamster. In the patch clamp experiments the calcium current with low amplitude 0.06±-0.87 nA and open probability 0.48% (±3.02 at -110 mV, n = 6, Ca2+ 2 mmol) was measured. HCN2-Ca2+ current significantly activated with cyclic adenosinemonophosphate by increasing of open probability and inhibited by specific If-blocker ivabradine. The calcium ion current was also shown in f-channels of neonatal rats ventriculocytes. Thus, the calcium ions conduction through HCN2 and native f-channel at physiological concentrations of extracellular fluid and physiological membrane potentials of cardiomyocytes was demonstrated.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1175-80.
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    ABSTRACT: Recent investigations have widened the knowledge concerning the brain derived neurotrophic factor (BDNF). The review concentrates on the data of literature and original data of authors on BDNF effects on genetically and epigenetically defined behavioral disorders. Specifically it describes the implication of the brain serotonergic system and the role of genotype in the neuroprotective effects of BDNF. The review provides evidence that: 1) genes encoding key elements of the brain serotonergic system (tryptophan hydroxylase-2, 5-HT1A and 5-HT2A receptors) are implicated in the effect ofBDNF; 2) acute central administration of BDNF produced long-term ameliorative effects on some animal models of genetically defined behavior disorders; 3) BDNF decreased behavioral disorders induced by prenatal stress and ethanol exposure and may play an important role in the treatment of epigenetically defined pathological behavior.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2013; 99(10):1125-37.
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    ABSTRACT: In the present work we investigated the effects of p53 inhibition by pifithrin-alpha (PFT) in vitro and in vivo on functioning vasopressinergic magnocellular neurones of rat hypothalamus. In vivo treatments with PFT were done by intra-hypothalamic microinjections or by intra peritoneal injections. In in vitro experiments hypothalamic slices containing supraoptic nuclei and intact pituitary were incubated with or without PFT. In all experiments we observed accumulation of vasopressin (VP) in the cell perikarya after PFT injections, however expression of VP mRNA was not changed. Analysis of VP content in the posterior pituitary demonstrated that amount of VP was significantly decreased after PFT treatments. Additionally, long-term inhibition of p53 in experiments with intra-hypothalamic injections of PFT resulted in an increased diuresis rate. The obtained results demonstrated that in all experiments PFT treatments inhibited VP anterograde transport from the cells of supraoptic nuclei. Moreover, analysis of MEK/ERK activities revealed that phosphorylation levels of MEK1/2 and ERK1/2 were decreased after PFT treatments. Our findings provide new evidences that p53 could be involved in the control of VP secretion from hypothalamo-hypophyseal system and that this action probably can be mediated by ERK signalling pathway.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 08/2013; 99(8):901-16.
  • Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 08/2013; 99(8):1002-4.
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    ABSTRACT: The effect of bacterial lipopolysaccharide (LPS) from E. coli on the viability of the cells of PC12 neuronal cell line was studied. LPS of 0111:B4 serotype was shown to have the most toxic effect on PC12 cells in the medium not containing serum, LPS of 055:B5 serotype was also found to be toxic. But the toxic effect of LPS of 0127:B8 serotype was not revealed in such medium. Preincubation of PC12 cells with GM1 and GD1a gangliosides at concentrations 50 or 100 μM diminishes significantly the death of the cells and the formation of reactive oxygen species induced by LPS of 0111:B4 and 055:B5 serotypes, but these gangliosides at nanomolar concentrations are not active. It was shown that the protective effect of GM1 and GD1a does not depend on the presence of the inhibitor of Trk receptor tyrosine kinase in the medium, their effect is similar to the effect of methyl-beta-cyclodextrin, which is known to disturb cell membrane raft structure. The data obtained suggest that the alteration of structural organization of lipid rafts in PC12 cell plasma membranes due to the incorporation of exogenous gangliosides in them prevents or diminishes the translocation and TLR4 receptor into rafts and their activation induced by LPS, it results in pronounced diminution of toxic effect of LPS on cells.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 07/2013;
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    ABSTRACT: In the experiments on the isolated perfused rat heart the effects of liposomes, containing different concentrations (0.25 and 0.1 mg/mL) of emoxipine, on coronary flow restoration after total normothermic ischemia and reperfusion were studied. The coronary flow, levels of nitrates and nitrites in the outflowing perfusate from heart and level of free radical processes were assessed, The obtained results showed that 0.1 mg/mL liposomal emoxipine provide with stronger increase coronary flow during reperfusion mostly due to the increase concentration of endothelial nitric oxide compare with treatments at 0.25 mg/mL.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 06/2013; 99(6):756-62.
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    ABSTRACT: Enzyme AMPK is a part of the family of serine/threonine specific protein kinases. AMPK plays important role in the transfer extracellular signals through phosphorylation of multiple substrates in different metabolic reactions of skeletal muscles. AMPK is geterotrimetric complex, consisting of the catalytic subunit (AMPKalpha) and two regulatory subunits (AMPKbeta and AMPKgamma), which are encoded by seven different high-homologous genes (alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3). AMPK regulates skeletal muscle metabolism through phosphorylation of various enzymes such as carbohydrate, lipid and protein metabolism, as well as factors of transcription and initiation. The AMPK expression occurs in response to a changing metabolic requests muscle cells and it leads to increased energy metabolism. The data of recent studies suggest the important role of AMPK in the regulation of intracellular metabolism and point to the need to study the molecular mechanisms involved in the regulation of gene expression in skeletal muscle.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 06/2013; 99(6):657-73.
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    ABSTRACT: It was demonstrated in experiments on rats, that increase of immobilization stress duration causes more prolonged activation of lipid peroxidation, however significant differences of both malonic dialdehyde and acylhydroperoxides content were not established in early period after stress in rats, which were exposed to the immobilization of different duration. It was shown, that increase of superoxiddismutase activity is observed in early period of 3-hour and 6-hour stress, but it was decreased in 12-hour immobilization. Catalase activity was decreased in 6-hour and 12-hour stress. These results confirm the literature data about dependence between the expression of stress manifestations and the strength and duration of stress factor action.
    Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 06/2013; 99(6):751-5.