Veterinární medicína (VET MED-CZECH )


Veterinarni Medicina is a veterinary journal from Czechoslovakia that highlights papers from veterinary researchers worldwide.

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  • Website
    Veterinarni Medicina website
  • Other titles
    Veterinární medicína (Online), Veterinary medicine
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    Document, Periodical, Internet resource
  • Document type
    Internet Resource, Computer File, Journal / Magazine / Newspaper

Publications in this journal

  • Veterinární medicína 06/2014; 44(7):205-2014.
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    ABSTRACT: e present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apop- tosis based on the detection of morphological changes. e neutrophils were isolated from mammary glands of five virgin heifers. e mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. e cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophil apoptosis and impaired cytoplasmic membrane integrity of neutrophils were already observed in the mammary gland lavages (11.9% and 0.8%, respectively). During the cultivation, a progressive increase in the number of apoptotic neutrophils in various stages of apoptosis - karyopyknosis, zeiosis and apoptotic bodies - was observed. Karyopyknotic neutrophils represented a dominant part of the apoptotic neutrophil population in the course of the whole cultivation. e most intensive increase was observed in zeiosis, whereas the levels of apoptotic bodies remained the same. After 4 hours of cultivation, 31.7% apoptotic neutrophils and 9.8% trypan blue positive neutrophils (i.e. Secondary necrotic cells) were found. e results of this work show that spontaneous apoptosis and secondary neutrophil necrosis must be taken into account during in vitro cultiva- tions of bovine mammary gland neutrophils.
    Veterinární medicína 06/2014;
  • Veterinární medicína 04/2014; 59(2):102-106.
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    ABSTRACT: The objective of this investigation was to study the histological structure of the gall bladder in the common quail (Coturnix coturnix). Samples were obtained from six male and six female healthy adult common quail. Tissue sections were stained with haematoxylin and eosin. Histological results showed that the overall histology of the gall bladder of the common quail is similar to that in the chukar partridge except for the goblet cells. No significant sex-based differences were found. The gall bladder was composed of tunica mucosa, tunica muscularis and tunica serosa or tunica adventitia. The tunica mucosa was mainly lined with simple columnar epithelium which was covered by a continuous striated border of microvilli. Goblet cells were observed among the epithelial cells. The tunica mucosa formed some folds which appeared to be regularly distributed over the whole gall bladder luminal surface and were mostly isometric. Deep invaginations of the surface epithelium were observed to have grown down into the underlying mesenchyme, showing a tubular gland-like appearance. The muscularis mucosa was absent. The thin lamina propria-submucosa contained loose connective tissue which consisted of numerous diffuse lymphatic tissues, but no glands were observed. The tunica muscularis consisted of a thin circular layer of smooth muscle fibres. The loose connective tissue of the tunica serosa or adventitia was made up of adipose tissues and blood vessels. It is concluded that the histological structures of the gall bladder of the common quail are similar to those of the chukar partridge except for the presence of goblet cells among the epithelial cells of the tunica mucosa.
    Veterinární medicína 01/2014; 59(5):261–264.

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