Current Eye Research (CURR EYE RES )

Publisher: Taylor & Francis

Description

The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and minireviews, all of high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.

Impact factor 1.66

  • Hide impact factor history
     
    Impact factor
  • 5-year impact
    1.70
  • Cited half-life
    9.80
  • Immediacy index
    0.30
  • Eigenfactor
    0.01
  • Article influence
    0.52
  • Website
    Current Eye Research website
  • Other titles
    Current eye research
  • ISSN
    0271-3683
  • OCLC
    6629649
  • Material type
    Periodical, Internet resource
  • Document type
    Journal / Magazine / Newspaper, Internet Resource

Publisher details

Taylor & Francis

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Some individual journals may have policies prohibiting pre-print archiving
    • On author's personal website or departmental website immediately
    • On institutional repository or subject-based repository after either 12 months embargo for STM, Behavioural Science and Public Health Journals or 18 months embargo for SSH journals
    • Publisher's version/PDF cannot be used
    • On a non-profit server
    • Published source must be acknowledged
    • Must link to publisher version
    • Set statements to accompany deposits (see policy)
    • The publisher will deposit in on behalf of authors to a designated institutional repository including PubMed Central, where a deposit agreement exists with the repository
    • STM: Science, Technology and Medicine
    • SSH: Social Science and Humanities
    • Publisher last contacted on 25/03/2014
    • 'Taylor & Francis (Psychology Press)' is an imprint of 'Taylor & Francis'
  • Classification
    ​ green

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: A predominant function of Müller cells is to regulate glutamate levels, but these cells are compromised in oxygen-induced retinopathy. The aim of this study was to investigate the role of pigment epithelium-derived factor (PEDF) in regulating glutamate levels in retina under hypoxia. Materials and methods: One-week-old C57BL/6J mice were exposed to 75% oxygen for 5 days and then kept in room air for another 5 days to establish the oxygen-induced retinopathy (OIR) mouse model. Mice received intravitreous injections of 2 μg PEDF or vehicle on postnatal (P)12 and P14, respectively. Antibody against interleukin-1Beta (IL-1β) (IL-1ab) was used to neutralize the activity of IL-1β, mice received intravitreous injections of 500 ng IL-1ab or vehicle on P12 and P14, respectively, too. At P17, the mice were euthanized and their eyes were enucleated. The expression levels of IL-1β, glutamine synthetase (GS) and l-glutamate/l-aspartate transporter (GLAST) in retinas with different treatments were detected. In addition, wild-type C57BL/6J mice received intravitreous injections of IL-1β or PEDF. After 24 h, the expression of GS and GLAST in the retinas was also detected. Furthermore, high-performance liquid chromatography (HPLC) was performed to determine the glutamate concentrations in retinas with different treatments. Results: The expression of IL-1β and levels of glutamate were increased in retinas with OIR, while the expression of GS and GLAST was decreased. Administration of PEDF ameliorated the characteristic changes in retinas of OIR mice. And neutralization of IL-1β by administration of IL-1ab increased GS and GLAST expression in retinas with OIR. Moreover, the effects of IL-1β on GS and GLAST expression and unbalanced glutamate levels were inhibited after receiving intravitreous injections of PEDF in retinas of normal mice. Conclusions: These results suggested that PEDF might up-regulate GS and GLAST expression and decrease glutamate levels by suppressing the role of IL-1β as an anti-inflammatory factor under hypoxia, and these functions may underlie the neuroprotective effects of PEDF.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: To evaluate the feasibility of retinal vein bypass surgery for induced branch retinal-vein occlusion (BRVO) in the living porcine eye. Methods: Fifteen minipigs were used in the study. Seven days before vascular surgery, hyaluronidase and plasmin were intravitreally injected for induction of posterior vitreous detachment. Aspirin and warfarin were oral administered daily starting 5 d prior to vascular surgery for anti-coagulation. The minipigs were anethetized with an intraperitoneal injection of 300 mg/kg chloral hydrate for intravitreal injection procedure and vascular surgery. Temporary keratoprosthesis vitrectomy was performed, and intraoperative video fluorescein angiography (VFA) was possible. The central and posterior vitreous was removed together with the posterior hyaloid membrane to facilitate vascular maneuvers. BRVO was induced by bipolar diathermy on the vein at the main vein's first branching. Polyimide tubes (50.8-μm internal diameter and 7.6-μm wall thickness) were used as artificial vessels. Vascular manipulation was performed in a bimanual manner. Both end of a prepared tubing was inserted into venous lumen by puncturing and catheterization, and the vein bypass bridging the occlusion was created. Then, the patency of the bypass graft was assessed by intraoperative VFA. Results: The retinal vein bypass surgery was surgically accomplished in 33% (5/15) of the eyes, and the immediate graft patency was confirmed by intraoperative VFA only in one eye. We observed and recorded fluorescein flow from the branch vein to the main vein through the bypass graft which bridging the occlusive vein segment. Conclusions: We demonstrated the feasibility of retinal vein bypass for induced BRVO in the living porcine eye, and the immediate graft patency was successfully evaluated by intraoperative VFA. Despite the potential, there are still some significant hurdles in vivo retinal vein bypass surgery, and modification of both surgical instruments and maneuvers is needed for further study.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: To identify the molecular basis and clinical phenotype in three Chinese families with hereditary cataracts. Methods: Detailed family history and clinical data were recorded. The phenotypes were documented using slit-lamp photography. Candidate genes sequencing was performed to screen out the disease causing mutation. Bioinformatics analysis was performed to predict the function of mutant genes. Results: The phenotypes of the families were identified as nuclear cataract in Family 1, pulverulent cataract in Family 2, and nuclear cataract in Family 3. Direct sequencing revealed transversions of C > T at c.218 (p. S73F) in GJA8 in Family 1, A > C at c.125 (p. E42A) in GJA3 in Family 2, and C > T at c.268 (p. L90F) in GJA3 in Family 3. These mutations co-segregated with all affected individuals in the family and were not found in unaffected family members nor in the 100 unrelated controls. Bioinformatics analysis indicated that S73F in GJA8, E42A and L90F in GJA3 are highly conserved. S73F in GJA8, E42A and L90F in GJA3 could possibly be damaging predicted by PolyPhen-2, with score of 0.858, 1.000, 1.000, respectively. Conclusions: This study identified three mutations in three Chinese families with hereditary cataracts. Of the three mutations, two were novel (c.125 A > C in GJA3 and c.268 C > T in GJA3), one was previously reported (c.218 C > T in GJA8).
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: Ab interno glaucoma surgery, such as trabecular aspiration or ab interno trabeculotomy, has been introduced as an alternative, non-filtering procedure to lower intraocular pressure in glaucoma patients. The purpose of the present study was to assess the feasibility of intraoperative spectral domain optical coherence tomography (iOCT) for improving ab interno glaucoma surgery. Materials and Methods: Intraoperative optical coherence tomography was performed to visualize the anterior chamber angle, the aspiration canula during trabecular aspiration, and the trabectome device during ab interno trabeculotomy. A commercially available 840-nm iOCT was used in a clinical setting, a 1310-nm spectral-domain OCT in an experimental setting. Results: In the clinical setting using the 840-nm iOCT, visualization of the aspiration canula and the trabectome device was possible, however, with rather limited visibility of the chamber angle and the trabecular meshwork. In the experimental setting, the 1310-nm OCT enabled excellent visualization of the chamber angle and detailed evaluation of the instrument-trabecular meshwork relation. Conclusions: Intraoperative use of optical coherence tomography may be helpful for ab interno glaucoma surgery. Adjustments of the commercially available iOCT, including longer wavelengths and oblique scanning options, are necessary for the clinical use.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Our eyes are, both literally and figuratively, windows to the world, and ophthalmic approaches offer a tremendous space for conducting research to learn more. Male/female differences in ocular health and disease are prevalent but we know far too little about root causes to design and implement diagnostic, preventive, and treatment strategies to address sex- and gender-based disparities in eye health. Herein, we discuss several ophthalmic diseases and other conditions with ocular manifestations, with a focus upon those that disproportionately affect women. Because the vast majority of biomedical research in this area comes from studies of mixed-gender populations, or of male-predominant populations, there is a pressing need for sex- and/or gender-based research at various points along the basic to clinical biomedical research continuum. Moreover, the multitude of factors that affect eye health call for a balanced look at the influence of biology, culture, and societal contributors. As clinicians, we owe our patients the best care for their needs, and that care must be derived from research that shows what is effective, for whom, and under what conditions.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this paper is to review male–female differences in the incidence and prevalence of diabetes and diabetic retinopathy. These differences will be established primarily through results from our present research and a review of related literature. Previously, we have demonstrated that neuroretinal dysfunction can be used to predict the location of future retinopathy up to three years before it is manifest. Our current research suggests that, for type 2 diabetes, the normal differences in neuroretinal function between nondiabetic males and females under 50 years of age are altered in patients with type 2 diabetes. Furthermore, local neuroretinal function in type 2 diabetes is more abnormal in adult males compared with adult females. The literature also suggests that there are male–female differences in the occurrence of diabetes. In adolescence, the incidence of type 1 diabetes is greater in males, whereas in type 2 diabetes, the incidence is greater in females. This excess of females in type 2 diabetes shifts to a more equal incidence between the two sexes in adults. In addition, advanced retinopathy in type 1 diabetes appears to be more common in males, and the presence and severity of diabetic retinopathy at the time of diagnosis in type 2 diabetes appears to be more associated with male sex. Although the reasons for male–female differences identified in this review are unknown, sex appears to be a significant factor in certain aspects of diabetes incidence and diabetic retinopathy.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: To compare intravitreal bevacizumab (IVB) and steroid activity in an experimental uveitis (EU) model. Materials and methods: EU was induced by intravitreal injection of bovine serum albumin (BSA) at baseline. Rabbits were randomly divided into three groups (n = 8) according to treatment with a single dose of IVB, steroid or saline on day 3. Aqueous humor (AH) samples were collected periodically (baseline and days 1, 3, 10 and 33). Rabbits were sacrified and eyes were enucleated for histopathology on day 33. AH vascular endothelial growth factor (VEGF) levels and histopathological changes were subjected to statistical analysis. Results: VEGF levels increased significantly after BSA injection (day 1) when compared with baseline in all three groups. Mean VEGF levels were 794.46 pg/mL, 777.91 pg/mL and 872 pg/mL for IVB, steroid and control groups, respectively (p = 0.002, p = 0.019 and p = 0.014). Seven days after treatment (day 10), VEGF levels decreased significantly in both treatment groups. Mean VEGF levels were 0 pg/mL, 292 pg/mL and 872 pg/mL, repectively (p < 0.001, p = 0.016 and p = 0.12). On day 33, the VEGF levels and inflammation had returned to baseline levels in both treatment groups, whereas no decrease in inflammation was observed in the control group. The histopathological evaluation revealed a mild congestion in ciliary tissue with a normal retina and choroidea in IVB group, a mild congestion in ciliary tissue with moderate inflammation in the posterior vitreus and a normal retina in steroid group and a severe inflammation in ciliary tissue with moderate inflammation in the retina in control group. Conclusions: These findings suggest that IVB may be used as an alternative to steroid treatment to reduce the acute effects of inflammation in patients with uveitis in whom intraocular steroids are contraindicated.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose/Aim of the Study: Photoreceptor degeneration is normally accompanied by reactive gliosis and gene expression changes in Müller (glial) cells. The signaling pathway involved inducing these changes in Müller cells is not known. It has been proposed that endothelin2 (EDN2) released by degenerating photoreceptors might induce gliotic changes in Müller cells. In the present study, we directly tested the hypothesis by determining whether treatment of Müller cell cultures with EDN2 results in upregulation of genes known to be expressed in activated Müller cells in vivo. Materials and Methods: Experiments were carried using an established rat Müller cell line (rMC-1), and gene expression was assessed by qRT-PCR. Results: We observed that EDN2 treatment upregulated transcripts for glial fibrillary acidic protein (Gfap), Serpina3n and endothelin receptor B (EdnrB), three genes associated with reactive gliosis in Müller cells. Ciliary neurotrophic factor (CNTF) treatment similarly led to induction of Gfap, Serpina3n and EdnrB transcripts, whereas glutamate treatment had no significant effect. Conclusions: The finding supports a role for EDN2 as a signaling agent between photoreceptors and Müller cells.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: It is essential to assess the yield of routine histopathological examination of pterygium specimens excised from clinically benign pterygium. The purpose of this study was to determine the rate of histopathologically identified ocular surface squamous neoplasia (OSSN) in clinically benign pterygium specimens. Materials and Methods: This retrospective consecutive study consisted of patients from the cornea services of the ophthalmology departments of Meir Medical Center, Kfar Sava, and Rabin Medical Center, Petah Tikva, Israel. Included patients were those with clinically benign pterygium who underwent pterygium excision between January 2002 and December 2011. The pterygium specimens were sent for histopathological examination and the pathology reports were reviewed for evidence of OSSN. Results: Overall, pathology reports of 682 eyes of 585 sequential patients were included in the study. There was a male predominance of cases (59%). Patients' age ranged from 16 to 87 years with an average age of 56 years. Six hundred and forty-three cases were primary (94%) and 39 cases were recurrent (6%). Conclusions: Cases of clinically benign pterygium diagnosed and excised by an experienced corneal expert are less likely to harbor OSSN. The yield of routine histopathological examination of such specimens may be low.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: Eliminating contamination by corneal stromal cells is critical when preparing cultured human corneal endothelial cells (CECs) transplantation. We investigated markers for the purification of cultured human CECs and markers for excluding cultured human corneal stromal myofibroblasts (CSMFs) from cultured human CECs. Materials and methods: CECs and CSMFs were obtained from human donor corneas by culturing separately in serum-containing medium. Candidate markers of CECs and CSMFs were screened with microarray analysis in the fourth passaged CECs and CSMFs. Then, selected factors were evaluated in reverse transcription polymerase chain reaction (RT-PCR), western blot, immunocytochemistry, and flow cytometry to investigate differential markers for each cell. Results: Among the genes identified by microarray analysis, cultured human CECs, but not CSMFs, expressed integrin alpha 3 (ITGA3 and CD49c) protein according to immunocytochemistry and western blotting. Iroquois homeobox 2 (IRX2) gene was a marker that distinguished CSMFs from cultured human CECs by RT-PCR. The IRX2 gene can be used as a marker of CSMFs contaminating cultured CECs. Conclusion: These molecules could be important markers for the production of highly purified cultured CECs for regenerative medicine.
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background: To evaluate the effects of selective α1A-adrenoceptor antagonist tamsulosin hydrochloride on choroidal thickness using enhanced depth imaging spectral-domain optical coherence tomography (EDI-OCT). Methods: This is a prospective observational study including 29 eyes of 29 patients with newly diagnosed benign prostatic hyperplasia. Choroidal thickness and retrobulbar ocular blood flow measurements were performed at baseline and after 3 months of treatment. Results were analyzed by the masked observer. Results: The mean subfoveal choroidal thickness (275.8–291.9 µm) and thicknesses 750 µm nasal (257.9–270.4 µm) and 750 µm temporal (262.4–277.0 µm) to the fovea were significantly increased after 3 months of treatment (p < 0.001). No statistically significant change was found in retrobulbar ocular blood flow. Conclusions: Tamsulosin causes a significant increase in EDI-OCT-based choroidal thickness measurements. This increase might be associated with choroidal vasodilation in consequence of blockade of sympathetic α1A-adrenoceptors, which is critical for the maintenance of vascular tone and resistance in the choroidal vascular architecture. This should be kept in mind when choroidal disease and its response to treatment are followed by EDI-OCT imaging. Read More: http://informahealthcare.com/doi/abs/10.3109/02713683.2014.971935
    Current Eye Research 12/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: Diabetes mellitus (DM) is characterized by high blood sugar levels over a prolonged period. Long term complications include but not limited heart disease, stroke, kidney failure, and ocular damage. An estimated 382 million people are diagnosed with Type 2 DM accounting for 90% of the cases. Common corneal dysfunctions associated with DM result in impaired vision due to decreased wound healing, corneal edema, and altered epithelial basement membrane. Lipids play a fundamental role in tissue metabolism and disease states. We attempt to determine the role of sphingolipids (SPL) in human Type I and Type II diabetic corneas. Materials and Methods: Cadaver corneas from healthy (non-diabetic/no ocular trauma), Type I (T1DM), and Type II diabetic (T2DM) donors were obtained and processed for lipidomics using LC-MS/MS. Results: Our data show significant differences in the SPL composition between control, T1DM and T2DM corneas. Both T1DM and T2DM showed a 10-folddownregulation of sphingomyelin(SM), 5-fold up regulation of Ceramides (Cer) and 2-fold upregulation of monohexosylceramides (MHC). Differences were also seen in total amounts of SPL where Cer was increased by approximately 3 fold in both T1DM and T2DM where SM decreased by 50% in both T1DM and T2DM when compared to healthy controls. No differences were seen in MHC amounts. Conclusions: Overall, our data indicate major differences in SPL distribution in human diabetic corneas. Information on the sphingolipids role in cornea, corneal cell physiology, and diseases are very limitedwhich highlights the importance of these findings.
    Current Eye Research 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: To examine the associations of various parameters of obesity including adiposity with intraocular pressure (IOP) using nationally representative data of South Korean adults. Material and methods: This cross-sectional study analyzed the data from the 2008-2010 Korea National Health and Nutrition Examination Survey. A total of 15,271 subjects (6600 men and 8671 women) participated. Body mass index (BMI), waist circumference (WC), total body fat mass, and total and regional body fat percentage were measured as parameters of obesity. Results: IOP showed positive linear associations with BMI, WC, total fat mass, and total and regional body fat percentages in men, and with BMI, WC, total fat mass, and trunk fat percentage in women after adjusting for confounding variables. Men with higher BMI, WC, total fat mass, and total and regional body fat percentages exhibited increasing trends in odd ratios for having IOP ≥ 18 mmHg after adjusting for all confounding factors (p for trend <0.001 for BMI and total fat mass; p for trend = 0.038 for WC; 0.003 for total body fat percentage; 0.002 for trunk fat percentage; 0.004 for leg fat percentage). However, only BMI showed a significantly increasing trend in the risk of IOP ≥18 mmHg in women. Conclusions: In addition to BMI, WC and total fat mass, total and regional body fat percentage in men and trunk fat percentage in women are positively associated with IOP. Increased BMI, WC, and total and regional body fat are positively associated with a risk of higher IOP (IOP ≥18 mmHg), especially in Korean men.
    Current Eye Research 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose/aim of study: Drug delivery to the ocular posterior segment is of importance, but it is a challenge in the treatment of irreversible blindness disease, such as age-related macular degeneration. Although some methods (i.e. intraocular injection, sustained release by polymer and iontophoresis) have been applied, some technical drawbacks, such as slow rate and damage to the eye, need to be overcome for wide use. Materials and methods: In this study, the feasibility of high-intensity focused ultrasound (HIFU) to enhance the transsclera drug delivery was tested for the first time. One-hundred HIFU pulses with the driving frequency of 1.1 MHz, acoustic power of 105.6 W, pulse duration of 10-50 ms and pulse repetition frequency of 1 Hz were delivered to the fresh ex vivo porcine sclera specimen. Results: In comparison to the passive diffusion (control), 50-ms HIFU can increase the penetration depth by 2.0 folds (501.7 ± 126.4 µm versus 252.4 ± 29.2 µm) using bicinchoninic acid assay and Rhodamine 6 G fluorescence intensity by 3.1 folds (22.4 ± 12.3 versus 7.1 ± 4.1) and coverage area by 2.6 folds (40.4 ± 9.1% versus 15.8 ± 2.9%). No morphological changes on the sonicated sclera samples were found using a surface electron microscope. Conclusions: In summary, pulsed-HIFU may be an effective modality in the transsclera drug delivery with a high transporting rate and depth. In vivo studies are necessary to further evaluate its performance, including the drug penetration and its possible side effects.
    Current Eye Research 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: The purpose of the present study was to investigate the profile of posterior precortical vitreous pocket (PPVP) in children using spectral-domain optical coherence tomography (OCT) and to compare the profile with that of adults. Materials and methods: In this retrospective comparative study, the height at the fovea and the horizontal diameter of PPVP in healthy children and adults in the upright position were measured based on OCT (Spectralis, Heidelberg Engineering GmbH, Heidelberg, Germany) images. PPVP diameter was divided into nasal length and temporal length based on the center of the fovea. The proportion of temporal length to the entire horizontal diameter was calculated as temporal length/horizontal diameter of PPVP. The association of age with the height and the proportion of temporal length to the entire horizontal diameter were evaluated in each group. The two values were also compared between the two groups. Results: Sixty-three eyes of 63 children (mean age: 6.9 ± 1.8 years) and 44 eyes of 44 young adults (mean age: 32.1 ± 5.3 years) were included. The height of PPVP at the fovea was 355.3 ± 80.3 µm in children and 367.8 ± 75.6 µm in adults. The proportion of temporal length to the entire horizontal diameter was 0.45 ± 0.05 and 0.50 ± 0.03. The proportion was significantly greater in adults than in children (p < 0.001), whereas the height (p = 0.349) was not different between the two groups. In children, there was a positive association between the age and the proportion of temporal length to the entire horizontal diameter (p = 0.001). Conclusion: The results suggest a progressive temporal extension of PPVP during childhood.
    Current Eye Research 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Purpose: To evaluate laser speckle flowgraphy (LSFG) measurements of the optic nerve head (ONH) blood flow (BF) response to hyperoxia. Methods: This prospective study included 30 eyes of 30 healthy volunteers (mean age: 28.5 ± 4.0, male:female = 13:17). The testing protocol had three phases: in the baseline phase, subjects breathed room air; in the hyperoxic phase, they breathed pure oxygen (6 L/min) for 15 min; and in the recovery phase, they were room air for 15 min. LSFG measurements of mean blur rate (MBR), which represents ONH BF, were taken every minute. The MBR ratio in the hyperoxic and recovery phases was calculated with reference to this baseline. Clinical parameters, including systemic blood pressure, pulse rate, and saturation of pulse-oximetry oxygen (SpO2), were measured every 5 min. Results: SpO2 increased significantly during hyperoxia (97.3 ± 1.1% to 99.3 ± 0.7%, p < 0.001). While clinical parameters were similar in hyperoxia and baseline, MBR decreased significantly after 2 min of hyperoxia (90.8 ± 12.6%, p = 0.02), stayed steady throughout hyperoxia (mean: 89.5 ± 10.8%, p range: <0.001-0.04) compared with baseline, and returned to baseline 1 min after recovery (93.7 ± 10.3%, p = 0.25). A linear regression using a third-order polynomial fitting curve analysis revealed that the time to reach minimum MBR was 7.78 min (adjusted R(2 )= 0.87). Conclusion: LSFG could effectively assess ONH BF changes during hyperoxia.
    Current Eye Research 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Objective: To investigate whether Ginkgo biloba extract retards selenite-induced cataractogenesis in Wistar rat pups. Methods: On postpartum day eight, Group I rat pups (n = 12) received an intraperitoneal injection of physiological saline. Groups II and III rat pups (n = 12) received a subcutaneous injection of sodium selenite. Group III also received an intraperitoneal injection of G. biloba extract once daily on postpartum days 9-14. Both eyes of each pup were examined from postpartum day 16 up to day 30. After sacrifice, encapsulated pup lens were analyzed for mean activities of catalase, superoxide dismutase, glutathione peroxidase (GPx), glutathione S-transferase and glutathione reductase. In addition, the mean concentrations of reduced glutathione (GSH) and malondialdehyde were analyzed in samples of lens and serum. Results: Dense lenticular opacification occurs 100% in Group II, but only minimal opacification occurs in three pups of Group III (25%), no opacification in 75% of Group III, none in Group I. Compared with Groups I and III, Group II rat showed lower lenticular antioxidant enzyme activity, lower level of GSH, and higher level of malondidehyde (mean ± standard deviation SD, p < 0.05 respectively). Conclusions: The treatment with G. biloba extract is effective against oxidative stress - a crucial factor of cataractogenesis in rat pups, possibly by preventing depletion of antioxidant enzymes and by inhibiting lipid peroxidation.
    Current Eye Research 11/2014;